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The map label for this gene is hpcB [H]

Identifier: 192290255

GI number: 192290255

Start: 1996630

End: 1997481

Strand: Reverse

Name: hpcB [H]

Synonym: Rpal_1859

Alternate gene names: 192290255

Gene position: 1997481-1996630 (Counterclockwise)

Preceding gene: 192290256

Following gene: 192290254

Centisome position: 34.77

GC content: 62.91

Gene sequence:

>852_bases
ATGGGCAAGCTGGTACTGGCGGCCAAAGTCAGTCACGTCCCCTCACTGATGCTGTCGGAGACCTCCGAAAGTCCGCTACG
TCAGGCACGGGCGGGCGCCGTCGCGGCGCTACGCGAACTTGGACGTCGCGCAAAAGAGCGCGAGGTCACCACCTTTGTGG
TGTTCGACACCCACTGGCTGTCGAACTTCGGCTATCACATCAACGCCAATGCGCAGCACCGCGGCTCGTTCACCAGCCAC
GAAGCGCCGCACATGATCCAGGACCTGCGCTACGACCTGCCGGGCGATCCGGCCTTAGCGCAGGCGATCGCCGCCGAGGC
ACAGAGTCACGGATTGAAGGTCCTGGCGCATCAGGTGCCGACGCTCGGGCTTGAATACGGCACGATCGTGCCGATGCACT
ATCTCAATCCCGACGGCTGGGCGAAGGTCGTCTCGATTGCTTCGCCACTGTTCACTTCGATCGAGGAGAGCCGCGCACTC
GGTGAAGCCACCCGCCACGCGATCGATGCCTCCGACGAACGCGTCGCGATCCTTGCTTCGGGCTCGCTGTCGCACCGGCT
GTGGCCGAACAAGGATCTCGGTCCCGATGCCTGGACCACCATCGCCAGTGAGTTCAATCGTCAGGTCGACCTGCGGGTGC
TGCAGCTTTGGCAGGAGCGGCGTTACCGCGAATTCGCCGCGATGCTCCCGGACTACGCGGTCAAGTGCAACGGCGAAGGC
GGCATGGCCGATACCGTGATGCTGTTCGCAGCGCTCGGGTGGGACGATTACACCGGCGAAGCCGAGCCATTGTGCGATTA
TTTCCCCTCCAGCGGCTCGGGCCAAGTCAATGTCGAATTTCATGTGAGTTAA

Upstream 100 bases:

>100_bases
ATGGGCGGTGACCAAGAAGCCGCCGCGCCACACCGCGTGGCTGCACCAGAAGGAATTCGCCGACGCCGAATAGCACCAGC
ACGACTCGGGAGTTGCACCG

Downstream 100 bases:

>100_bases
TTTGCACGTGTATTCAGCGCTCAGCACCGCCCCGGCGCCTTGACAAACCGCCTCGTCCCCGGCGGAATCCGCTATAATTA
CGCCGCTACTCAAGCCGCGG

Product: 3,4-dihydroxyphenylacetate 2,3-dioxygenase

Products: NA

Alternate protein names: Homoprotocatechuate 2,3-dioxygenase; HPC dioxygenase [H]

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MGKLVLAAKVSHVPSLMLSETSESPLRQARAGAVAALRELGRRAKEREVTTFVVFDTHWLSNFGYHINANAQHRGSFTSH
EAPHMIQDLRYDLPGDPALAQAIAAEAQSHGLKVLAHQVPTLGLEYGTIVPMHYLNPDGWAKVVSIASPLFTSIEESRAL
GEATRHAIDASDERVAILASGSLSHRLWPNKDLGPDAWTTIASEFNRQVDLRVLQLWQERRYREFAAMLPDYAVKCNGEG
GMADTVMLFAALGWDDYTGEAEPLCDYFPSSGSGQVNVEFHVS

Sequences:

>Translated_283_residues
MGKLVLAAKVSHVPSLMLSETSESPLRQARAGAVAALRELGRRAKEREVTTFVVFDTHWLSNFGYHINANAQHRGSFTSH
EAPHMIQDLRYDLPGDPALAQAIAAEAQSHGLKVLAHQVPTLGLEYGTIVPMHYLNPDGWAKVVSIASPLFTSIEESRAL
GEATRHAIDASDERVAILASGSLSHRLWPNKDLGPDAWTTIASEFNRQVDLRVLQLWQERRYREFAAMLPDYAVKCNGEG
GMADTVMLFAALGWDDYTGEAEPLCDYFPSSGSGQVNVEFHVS
>Mature_282_residues
GKLVLAAKVSHVPSLMLSETSESPLRQARAGAVAALRELGRRAKEREVTTFVVFDTHWLSNFGYHINANAQHRGSFTSHE
APHMIQDLRYDLPGDPALAQAIAAEAQSHGLKVLAHQVPTLGLEYGTIVPMHYLNPDGWAKVVSIASPLFTSIEESRALG
EATRHAIDASDERVAILASGSLSHRLWPNKDLGPDAWTTIASEFNRQVDLRVLQLWQERRYREFAAMLPDYAVKCNGEGG
MADTVMLFAALGWDDYTGEAEPLCDYFPSSGSGQVNVEFHVS

Specific function: Transforms homoprotocatechuic acid (HPC) into 5- carboxymethyl-2-hydroxy-muconic semialdehyde (CHMS) [H]

COG id: COG3384

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011984
- InterPro:   IPR004183 [H]

Pfam domain/function: PF02900 LigB [H]

EC number: =1.13.11.15 [H]

Molecular weight: Translated: 31110; Mature: 30979

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKLVLAAKVSHVPSLMLSETSESPLRQARAGAVAALRELGRRAKEREVTTFVVFDTHWL
CCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECHHH
SNFGYHINANAQHRGSFTSHEAPHMIQDLRYDLPGDPALAQAIAAEAQSHGLKVLAHQVP
HCCCEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHCC
TLGLEYGTIVPMHYLNPDGWAKVVSIASPLFTSIEESRALGEATRHAIDASDERVAILAS
CCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEC
GSLSHRLWPNKDLGPDAWTTIASEFNRQVDLRVLQLWQERRYREFAAMLPDYAVKCNGEG
CCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
GMADTVMLFAALGWDDYTGEAEPLCDYFPSSGSGQVNVEFHVS
CCHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCEEEEEEEEC
>Mature Secondary Structure 
GKLVLAAKVSHVPSLMLSETSESPLRQARAGAVAALRELGRRAKEREVTTFVVFDTHWL
CCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECHHH
SNFGYHINANAQHRGSFTSHEAPHMIQDLRYDLPGDPALAQAIAAEAQSHGLKVLAHQVP
HCCCEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHCC
TLGLEYGTIVPMHYLNPDGWAKVVSIASPLFTSIEESRALGEATRHAIDASDERVAILAS
CCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEC
GSLSHRLWPNKDLGPDAWTTIASEFNRQVDLRVLQLWQERRYREFAAMLPDYAVKCNGEG
CCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
GMADTVMLFAALGWDDYTGEAEPLCDYFPSSGSGQVNVEFHVS
CCHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2261999 [H]