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The map label for this gene is gatA [H]

Identifier: 192290050

GI number: 192290050

Start: 1770388

End: 1772193

Strand: Reverse

Name: gatA [H]

Synonym: Rpal_1651

Alternate gene names: 192290050

Gene position: 1772193-1770388 (Counterclockwise)

Preceding gene: 192290051

Following gene: 192290048

Centisome position: 30.85

GC content: 68.88

Gene sequence:

>1806_bases
ATGAGCCAATTCGAAACCATCAGTGAAATCGTCGCCGCGCATCGCGCCGGCACCACCACCCCCGCGCAGACGATCGCGCG
CTGCTACCAGCGCATCCGCGCCTATGCCGATCCGGCACTGTTTATCACCCTGCGCGATGAGGCCGATGCGATCGCCGAGG
CGGTCGCGGTGGCGGCGCGCGACCCGTCGCTGCCGCTGTACGGCGTGCCGGTCGCAGTGAAGGACAATATCGACGTTGCC
GGCCTGACGACCACCGCCGCCTGCCCGGCATTTGCATATCAGCCGGCCCACGACGCCACCGCGGTCGCCAAGCTGCGCGC
CGCCGGCGCGATCGTGATCGGCAAGACCAATCTCGATCAGTTCGCCACTGGTCTCGTCGGCGTGCGCTCGCCTTACGGCG
TCCCCCGCAACGCGATGCGCAGCGACCTCGTCCCCGGCGGATCGAGCTCCGGCTCGGCGGTCGCGGTCGGTGCCGGGCTG
GTGCCGCTGTCGCTCGGCACCGATACGGCGGGCTCGGGCCGGGTGCCGGCGATGCTCAACAACATCGTTGGGCTGAAGCC
GAGCCTCGGGATGATTTCGACCACAGGTGTCGTGCCGGCGTGCCGCACGCTCGACTGCGTCTCGATCTTCGCATTGACGA
CCGATGACGCGATGACCGCGCTGCGGGTGATGACGGGGCCAGATGCCGAAGATCCGTTCTCGCGGGAACGGCCGGTGGCG
GCAGTCACCGCGATCCCGAAGCGCGTGCGGCTCGGCGTGCCGCCGCAAGATCAATTGCAGTTCTTCGGCGATGATCTCGC
GGCGCGTGGTTATGAGGAAGCGGTCGAGCGCTGGCGAAAGCTCGGTGCGGAGCTGGTCGAGATCGATGTCGAACCGCTCT
ACGAGACGGCGCGGCTGCTGTACGAAGGGCCATGGGTGGCGGAGCGCTATCTCACCATCCGGGAGTTGCTGGAGACCCAG
CCGGACGCGGTGCACCCGGTGACGCGGCAGATCACCCTCGCCGGTGCCAAGCTGTCCGCGGCAGACACCTTCGCGGCGCT
GTACCGGCTGCAGGCGTTACGTAAGATCGCCGAGCACAGCTTCGCCGGCATCGACGCGCTGGTGCTGCCGACCGCGCCGA
CCGCCTACACGGTCGAACAGGTGCTGACCGATCCGATCACGCTGAACAGCCGGCTCGGCACCTACACCAACTTCGTCAAC
CTGCTGGACCTGTGCGGCCTCGCGCTGCCGGCGTCGATCCGCAGCGACGGCATTCCGTTCGGCATCACCCTGCTGGCGCC
TGCCGGCCGCGATGCCGAACTTGCCGGCCTCGGCCGCGTGTTCCACGCCGACACCGCGCTGCCGATGGGCGCCAGCGGCC
AGCCCCAGCCGCCGCTCGCCGAAGTCACTGGCGGTGATGCGCCGGGTGAGATCGCGATCGCGGTGGTCGGAGCGCATCTT
TCCGGTATGCCGTTGAACCGCGAGCTGACCGCACTCGGTGGCCGACTTCTGTCGGCCACCGCCACAGCGCCGGACTACAA
ACTCTATGCGCTGAAAGGCACCGTGCCGCCGAAGCCCGGCCTGCTGCGCGTGGCGCCGGGTAGTGGTTCTGCGATCGCTG
TAGAAGTGTGGGCGCTGTCGCCGGCCGCATTCGGCTCGTTCGTCGCGGCAATCCCATCGCCGCTGTCGATCGGTACGCTG
ACGCTCGCCGACGGCACCGCGGTGAAGGGCTTCCTCACCGAGCCGGCGGCGATCGAAGGCGCCCGCGATATCTCGCATTT
CGGCGGCTGGCGCGCCTACATGGCGGAGCTGGCGGCGACCGGCTGA

Upstream 100 bases:

>100_bases
TGATCGCGATCCTGCGCGGCGACCGCGACGGCGCCGCCAAAGCGATGCGCCATCACATCGATCTGGTGCGCGACGAATAC
GAAATCTACGCGGTGTCGGT

Downstream 100 bases:

>100_bases
ACTGTTGCCGCGATCAGCGGAAGAACACTTCCTGATTGCGGCGCTCGAGCTCGGTCGAATAGGTCCGCAGCACGTGCGCC
TCGCTCACGGTGCCGATCGC

Product: allophanate hydrolase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 601; Mature: 600

Protein sequence:

>601_residues
MSQFETISEIVAAHRAGTTTPAQTIARCYQRIRAYADPALFITLRDEADAIAEAVAVAARDPSLPLYGVPVAVKDNIDVA
GLTTTAACPAFAYQPAHDATAVAKLRAAGAIVIGKTNLDQFATGLVGVRSPYGVPRNAMRSDLVPGGSSSGSAVAVGAGL
VPLSLGTDTAGSGRVPAMLNNIVGLKPSLGMISTTGVVPACRTLDCVSIFALTTDDAMTALRVMTGPDAEDPFSRERPVA
AVTAIPKRVRLGVPPQDQLQFFGDDLAARGYEEAVERWRKLGAELVEIDVEPLYETARLLYEGPWVAERYLTIRELLETQ
PDAVHPVTRQITLAGAKLSAADTFAALYRLQALRKIAEHSFAGIDALVLPTAPTAYTVEQVLTDPITLNSRLGTYTNFVN
LLDLCGLALPASIRSDGIPFGITLLAPAGRDAELAGLGRVFHADTALPMGASGQPQPPLAEVTGGDAPGEIAIAVVGAHL
SGMPLNRELTALGGRLLSATATAPDYKLYALKGTVPPKPGLLRVAPGSGSAIAVEVWALSPAAFGSFVAAIPSPLSIGTL
TLADGTAVKGFLTEPAAIEGARDISHFGGWRAYMAELAATG

Sequences:

>Translated_601_residues
MSQFETISEIVAAHRAGTTTPAQTIARCYQRIRAYADPALFITLRDEADAIAEAVAVAARDPSLPLYGVPVAVKDNIDVA
GLTTTAACPAFAYQPAHDATAVAKLRAAGAIVIGKTNLDQFATGLVGVRSPYGVPRNAMRSDLVPGGSSSGSAVAVGAGL
VPLSLGTDTAGSGRVPAMLNNIVGLKPSLGMISTTGVVPACRTLDCVSIFALTTDDAMTALRVMTGPDAEDPFSRERPVA
AVTAIPKRVRLGVPPQDQLQFFGDDLAARGYEEAVERWRKLGAELVEIDVEPLYETARLLYEGPWVAERYLTIRELLETQ
PDAVHPVTRQITLAGAKLSAADTFAALYRLQALRKIAEHSFAGIDALVLPTAPTAYTVEQVLTDPITLNSRLGTYTNFVN
LLDLCGLALPASIRSDGIPFGITLLAPAGRDAELAGLGRVFHADTALPMGASGQPQPPLAEVTGGDAPGEIAIAVVGAHL
SGMPLNRELTALGGRLLSATATAPDYKLYALKGTVPPKPGLLRVAPGSGSAIAVEVWALSPAAFGSFVAAIPSPLSIGTL
TLADGTAVKGFLTEPAAIEGARDISHFGGWRAYMAELAATG
>Mature_600_residues
SQFETISEIVAAHRAGTTTPAQTIARCYQRIRAYADPALFITLRDEADAIAEAVAVAARDPSLPLYGVPVAVKDNIDVAG
LTTTAACPAFAYQPAHDATAVAKLRAAGAIVIGKTNLDQFATGLVGVRSPYGVPRNAMRSDLVPGGSSSGSAVAVGAGLV
PLSLGTDTAGSGRVPAMLNNIVGLKPSLGMISTTGVVPACRTLDCVSIFALTTDDAMTALRVMTGPDAEDPFSRERPVAA
VTAIPKRVRLGVPPQDQLQFFGDDLAARGYEEAVERWRKLGAELVEIDVEPLYETARLLYEGPWVAERYLTIRELLETQP
DAVHPVTRQITLAGAKLSAADTFAALYRLQALRKIAEHSFAGIDALVLPTAPTAYTVEQVLTDPITLNSRLGTYTNFVNL
LDLCGLALPASIRSDGIPFGITLLAPAGRDAELAGLGRVFHADTALPMGASGQPQPPLAEVTGGDAPGEIAIAVVGAHLS
GMPLNRELTALGGRLLSATATAPDYKLYALKGTVPPKPGLLRVAPGSGSAIAVEVWALSPAAFGSFVAAIPSPLSIGTLT
LADGTAVKGFLTEPAAIEGARDISHFGGWRAYMAELAATG

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=501, Percent_Identity=26.1477045908184, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI195972892, Length=228, Percent_Identity=28.5087719298246, Blast_Score=81, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17543272, Length=443, Percent_Identity=27.5395033860045, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17537465, Length=461, Percent_Identity=24.5119305856833, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17556264, Length=289, Percent_Identity=27.3356401384083, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI71990152, Length=318, Percent_Identity=28.3018867924528, Blast_Score=82, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17538252, Length=173, Percent_Identity=31.2138728323699, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319685, Length=550, Percent_Identity=45.0909090909091, Blast_Score=430, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6323950, Length=232, Percent_Identity=32.3275862068966, Blast_Score=90, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24648113, Length=298, Percent_Identity=33.5570469798658, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24652985, Length=445, Percent_Identity=23.8202247191011, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19922090, Length=445, Percent_Identity=23.8202247191011, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24652981, Length=445, Percent_Identity=23.8202247191011, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24652983, Length=445, Percent_Identity=23.8202247191011, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI45550774, Length=188, Percent_Identity=30.3191489361702, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24648435, Length=188, Percent_Identity=30.3191489361702, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24648437, Length=188, Percent_Identity=30.3191489361702, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24648441, Length=191, Percent_Identity=29.3193717277487, Blast_Score=67, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24648439, Length=191, Percent_Identity=29.3193717277487, Blast_Score=67, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 62397; Mature: 62266

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQFETISEIVAAHRAGTTTPAQTIARCYQRIRAYADPALFITLRDEADAIAEAVAVAAR
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHCC
DPSLPLYGVPVAVKDNIDVAGLTTTAACPAFAYQPAHDATAVAKLRAAGAIVIGKTNLDQ
CCCCCEEECCEEEECCCCEECCCHHHCCCCCCCCCCCCHHHHHHHHHCCEEEEECCCHHH
FATGLVGVRSPYGVPRNAMRSDLVPGGSSSGSAVAVGAGLVPLSLGTDTAGSGRVPAMLN
HHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCEEEEECCEEEEECCCCCCCCCCCHHHHH
NIVGLKPSLGMISTTGVVPACRTLDCVSIFALTTDDAMTALRVMTGPDAEDPFSRERPVA
HHHCCCCCCCCEECCCCCCCHHHHCEEEEEEECCCHHHHHHHEECCCCCCCCCCCCCCCC
AVTAIPKRVRLGVPPQDQLQFFGDDLAARGYEEAVERWRKLGAELVEIDVEPLYETARLL
HHHHCCHHEECCCCCHHHHHHHCCHHHHCCHHHHHHHHHHCCCEEEEECCHHHHHHHHHH
YEGPWVAERYLTIRELLETQPDAVHPVTRQITLAGAKLSAADTFAALYRLQALRKIAEHS
HCCCCHHHHHHHHHHHHHCCCCCCCCCHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
FAGIDALVLPTAPTAYTVEQVLTDPITLNSRLGTYTNFVNLLDLCGLALPASIRSDGIPF
CCCCCEEEECCCCCHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCE
GITLLAPAGRDAELAGLGRVFHADTALPMGASGQPQPPLAEVTGGDAPGEIAIAVVGAHL
EEEEECCCCCCCHHHHCCHHEECCCCCCCCCCCCCCCCCHHCCCCCCCCCEEEEEEECCC
SGMPLNRELTALGGRLLSATATAPDYKLYALKGTVPPKPGLLRVAPGSGSAIAVEVWALS
CCCCCCCHHHHHCCCCEEEECCCCCEEEEEEECCCCCCCCEEEECCCCCCEEEEEEEECC
PAAFGSFVAAIPSPLSIGTLTLADGTAVKGFLTEPAAIEGARDISHFGGWRAYMAELAAT
HHHHHHHHHHCCCCCCCCEEEECCCCEEEEECCCCHHHCCCHHHHHHCHHHHHHHHHHCC
G
C
>Mature Secondary Structure 
SQFETISEIVAAHRAGTTTPAQTIARCYQRIRAYADPALFITLRDEADAIAEAVAVAAR
CHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHCC
DPSLPLYGVPVAVKDNIDVAGLTTTAACPAFAYQPAHDATAVAKLRAAGAIVIGKTNLDQ
CCCCCEEECCEEEECCCCEECCCHHHCCCCCCCCCCCCHHHHHHHHHCCEEEEECCCHHH
FATGLVGVRSPYGVPRNAMRSDLVPGGSSSGSAVAVGAGLVPLSLGTDTAGSGRVPAMLN
HHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCEEEEECCEEEEECCCCCCCCCCCHHHHH
NIVGLKPSLGMISTTGVVPACRTLDCVSIFALTTDDAMTALRVMTGPDAEDPFSRERPVA
HHHCCCCCCCCEECCCCCCCHHHHCEEEEEEECCCHHHHHHHEECCCCCCCCCCCCCCCC
AVTAIPKRVRLGVPPQDQLQFFGDDLAARGYEEAVERWRKLGAELVEIDVEPLYETARLL
HHHHCCHHEECCCCCHHHHHHHCCHHHHCCHHHHHHHHHHCCCEEEEECCHHHHHHHHHH
YEGPWVAERYLTIRELLETQPDAVHPVTRQITLAGAKLSAADTFAALYRLQALRKIAEHS
HCCCCHHHHHHHHHHHHHCCCCCCCCCHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
FAGIDALVLPTAPTAYTVEQVLTDPITLNSRLGTYTNFVNLLDLCGLALPASIRSDGIPF
CCCCCEEEECCCCCHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCE
GITLLAPAGRDAELAGLGRVFHADTALPMGASGQPQPPLAEVTGGDAPGEIAIAVVGAHL
EEEEECCCCCCCHHHHCCHHEECCCCCCCCCCCCCCCCCHHCCCCCCCCCEEEEEEECCC
SGMPLNRELTALGGRLLSATATAPDYKLYALKGTVPPKPGLLRVAPGSGSAIAVEVWALS
CCCCCCCHHHHHCCCCEEEECCCCCEEEEEEECCCCCCCCEEEECCCCCCEEEEEEEECC
PAAFGSFVAAIPSPLSIGTLTLADGTAVKGFLTEPAAIEGARDISHFGGWRAYMAELAAT
HHHHHHHHHHCCCCCCCCEEEECCCCEEEEECCCCHHHCCCHHHHHHCHHHHHHHHHHCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12566566 [H]