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The map label for this gene is mcbR [H]

Identifier: 192290051

GI number: 192290051

Start: 1772190

End: 1772936

Strand: Reverse

Name: mcbR [H]

Synonym: Rpal_1652

Alternate gene names: 192290051

Gene position: 1772936-1772190 (Counterclockwise)

Preceding gene: 192290052

Following gene: 192290050

Centisome position: 30.87

GC content: 65.33

Gene sequence:

>747_bases
ATGAGCTTCGATGATGCGCTGCCATCACGCCGAACCAAGTCACCTCTCGTGCCGCCGGGAGTGATCACTCCGAACACGCG
GATGGTGCGGGCCGCGCCCTCTCCAATCAAGATCACCCGCGCCGAGGAGCTGCGGCGCCAGCTTGCCGACGAGATCGTGT
GCGGCACGCTGCGCCCCGGCGCAGCACTCGACGAAACCGACATCGCACAGCGTTTCAAGGTCTCTCGCACGCCGGTGCGC
GAGGCACTGCGGCAGCTCGTCGCCAGTGGCCTCGTCGAGTCGCGGCCGCATCACGGCGCGGTGGTGGCGCAGCCGTCATT
CGAACGGCTGACTGGTATGTTCGAAGCGATGTCGGAGCTCGAAGCACTGTGTGCGAGCTTTGCGGCCGAGCGGATGACTC
CGCTGGAGCGCCGGACGCTGGAGACGATTCACGAAGAGCTGCGGATGCTCAGCTCGCAGGGCAATCCGGAACGCTTCCAC
GACGTCAACGAGCACTTTCACAACGCGATCTATGCGGGGGCACACAATGACTACATCGCCGAGATCACGCTGGCGACGCG
GGTGCGGGTGCAGCCGTTCCGCCGTGCCCAGTTCCGCAATCTCGGACGGCTGGCGAAGTCGCATGCCGAGCACGATCGCG
TGGTGATCGCGATCCTGCGCGGCGACCGCGACGGCGCCGCCAAAGCGATGCGCCATCACATCGATCTGGTGCGCGACGAA
TACGAAATCTACGCGGTGTCGGTATGA

Upstream 100 bases:

>100_bases
GCAAGGTCGGACGGCAGATGCAGACCTGGGTGCGGTTTGCCGAAGGCTGGCGCATCGTCGCGGCCCATGTCAGCATGATC
GACGAACCGCGGGCGTCGCC

Downstream 100 bases:

>100_bases
GCCAATTCGAAACCATCAGTGAAATCGTCGCCGCGCATCGCGCCGGCACCACCACCCCCGCGCAGACGATCGCGCGCTGC
TACCAGCGCATCCGCGCCTA

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MSFDDALPSRRTKSPLVPPGVITPNTRMVRAAPSPIKITRAEELRRQLADEIVCGTLRPGAALDETDIAQRFKVSRTPVR
EALRQLVASGLVESRPHHGAVVAQPSFERLTGMFEAMSELEALCASFAAERMTPLERRTLETIHEELRMLSSQGNPERFH
DVNEHFHNAIYAGAHNDYIAEITLATRVRVQPFRRAQFRNLGRLAKSHAEHDRVVIAILRGDRDGAAKAMRHHIDLVRDE
YEIYAVSV

Sequences:

>Translated_248_residues
MSFDDALPSRRTKSPLVPPGVITPNTRMVRAAPSPIKITRAEELRRQLADEIVCGTLRPGAALDETDIAQRFKVSRTPVR
EALRQLVASGLVESRPHHGAVVAQPSFERLTGMFEAMSELEALCASFAAERMTPLERRTLETIHEELRMLSSQGNPERFH
DVNEHFHNAIYAGAHNDYIAEITLATRVRVQPFRRAQFRNLGRLAKSHAEHDRVVIAILRGDRDGAAKAMRHHIDLVRDE
YEIYAVSV
>Mature_247_residues
SFDDALPSRRTKSPLVPPGVITPNTRMVRAAPSPIKITRAEELRRQLADEIVCGTLRPGAALDETDIAQRFKVSRTPVRE
ALRQLVASGLVESRPHHGAVVAQPSFERLTGMFEAMSELEALCASFAAERMTPLERRTLETIHEELRMLSSQGNPERFHD
VNEHFHNAIYAGAHNDYIAEITLATRVRVQPFRRAQFRNLGRLAKSHAEHDRVVIAILRGDRDGAAKAMRHHIDLVRDEY
EIYAVSV

Specific function: Important for biofilm formation. Represses expression of mcbA by binding to its promoter region, which prevents colanic acid overproduction and mucoidy [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081914, Length=206, Percent_Identity=26.6990291262136, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI87082149, Length=198, Percent_Identity=28.2828282828283, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1787821, Length=193, Percent_Identity=24.8704663212435, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 27914; Mature: 27783

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFDDALPSRRTKSPLVPPGVITPNTRMVRAAPSPIKITRAEELRRQLADEIVCGTLRPG
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEECHHHHHHHHHHHHHHCCCCCC
AALDETDIAQRFKVSRTPVREALRQLVASGLVESRPHHGAVVAQPSFERLTGMFEAMSEL
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHH
EALCASFAAERMTPLERRTLETIHEELRMLSSQGNPERFHDVNEHFHNAIYAGAHNDYIA
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEECCCCCCEEE
EITLATRVRVQPFRRAQFRNLGRLAKSHAEHDRVVIAILRGDRDGAAKAMRHHIDLVRDE
EEEHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCC
YEIYAVSV
EEEEEEEC
>Mature Secondary Structure 
SFDDALPSRRTKSPLVPPGVITPNTRMVRAAPSPIKITRAEELRRQLADEIVCGTLRPG
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEECHHHHHHHHHHHHHHCCCCCC
AALDETDIAQRFKVSRTPVREALRQLVASGLVESRPHHGAVVAQPSFERLTGMFEAMSEL
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHH
EALCASFAAERMTPLERRTLETIHEELRMLSSQGNPERFHDVNEHFHNAIYAGAHNDYIA
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEECCCCCCEEE
EITLATRVRVQPFRRAQFRNLGRLAKSHAEHDRVVIAILRGDRDGAAKAMRHHIDLVRDE
EEEHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCC
YEIYAVSV
EEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]