Click here to switch to the map view.

The map label for this gene is puuD [H]

Identifier: 192290048

GI number: 192290048

Start: 1767511

End: 1768314

Strand: Reverse

Name: puuD [H]

Synonym: Rpal_1649

Alternate gene names: 192290048

Gene position: 1768314-1767511 (Counterclockwise)

Preceding gene: 192290050

Following gene: 192290046

Centisome position: 30.79

GC content: 67.41

Gene sequence:

>804_bases
ATGACTGGCAGAGCGGTGATCGGCGTGATCGGCAACGCCCATCTCGTCGATAATCGGTTTGCCGTCCAGCATGTGGGCGA
GCGCAACCTGCGCGCGGTGGCCGAGGTTGCCGGCGCGCTGCCGCTGATGTTCGGCGGCTCGTCAGAGTTCGCCCAGATCG
AGGACCTGCTGGAGGTGGTCGACGGCATCCTGCTCACCGGCGGCCGGGCCAACGTGCATCCGACCTACTTCAAGACCGAG
CCCAATCCGCGCCACGAGCCCTACGACCAGCCGCGCGACGAGCTGGCGCTGGCGCTCGTGCGTACCTGCGTCGAGCGCGG
TGTACCGCTGTTCGGGATCTGCCGCGGCTTTCAGGAGATGAACGTCGCCTATGGCGGCTCGCTGCATCCGGAGATTCGCG
AGCTGCCGGGGCGGATGAACCACCGCATGCCGCGGCTGGAGAATGGCGAGATCCATCCCGATCCGACCGTTGTGTTCGCC
GATCGCCACGACGTGCAGCTTACGTCCGATGGCGTGTTCGCACGCCTGCTCGGCCGCGATCTGATCCGGGTGAATTCGTT
GCATGGCCAGGGCATTCTTGAGCCCGGCCCGCGCATTGTGATCGAAGGCGTCGCCGAGGACGGCACCATCGAGGCGATCC
GCATTGCCGATGCCCCCGGCTTCGCGCTCGGCGTGCAGTGGCACGCCGAATACGATCCGCAGGTCAATCCGATCAACCGC
GCGCTGTTTGTCGCCTTCGGCGAAGCGGTCTCCGCCTATCGCACGGCGCGGCAGCGGCCGGCGGCGTTGCGGCGGTCGGG
CTGA

Upstream 100 bases:

>100_bases
GATCGCCAGTGCAGAGCTGGCAACCAATTAAGGCAGCAGCGTCGGGCTCGATTTTCGCCGTTTTGGCTGTATGGTCCGAG
GGTTGTCAGGGAGGAACAGG

Downstream 100 bases:

>100_bases
CACAAACTGTTAGTTCGTTCCTGAGCTACGTCATGGCCGGGCTTGTCCCGGCCATCCACGTCTTTGTTGCGACCTCGTGG
ATGCCCGGCACAAAGGCCGG

Product: peptidase C26

Products: NA

Alternate protein names: Gamma-Glu-GABA hydrolase [H]

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MTGRAVIGVIGNAHLVDNRFAVQHVGERNLRAVAEVAGALPLMFGGSSEFAQIEDLLEVVDGILLTGGRANVHPTYFKTE
PNPRHEPYDQPRDELALALVRTCVERGVPLFGICRGFQEMNVAYGGSLHPEIRELPGRMNHRMPRLENGEIHPDPTVVFA
DRHDVQLTSDGVFARLLGRDLIRVNSLHGQGILEPGPRIVIEGVAEDGTIEAIRIADAPGFALGVQWHAEYDPQVNPINR
ALFVAFGEAVSAYRTARQRPAALRRSG

Sequences:

>Translated_267_residues
MTGRAVIGVIGNAHLVDNRFAVQHVGERNLRAVAEVAGALPLMFGGSSEFAQIEDLLEVVDGILLTGGRANVHPTYFKTE
PNPRHEPYDQPRDELALALVRTCVERGVPLFGICRGFQEMNVAYGGSLHPEIRELPGRMNHRMPRLENGEIHPDPTVVFA
DRHDVQLTSDGVFARLLGRDLIRVNSLHGQGILEPGPRIVIEGVAEDGTIEAIRIADAPGFALGVQWHAEYDPQVNPINR
ALFVAFGEAVSAYRTARQRPAALRRSG
>Mature_266_residues
TGRAVIGVIGNAHLVDNRFAVQHVGERNLRAVAEVAGALPLMFGGSSEFAQIEDLLEVVDGILLTGGRANVHPTYFKTEP
NPRHEPYDQPRDELALALVRTCVERGVPLFGICRGFQEMNVAYGGSLHPEIRELPGRMNHRMPRLENGEIHPDPTVVFAD
RHDVQLTSDGVFARLLGRDLIRVNSLHGQGILEPGPRIVIEGVAEDGTIEAIRIADAPGFALGVQWHAEYDPQVNPINRA
LFVAFGEAVSAYRTARQRPAALRRSG

Specific function: Involved in the breakdown of putrescine via hydrolysis of the gamma-glutamyl linkage of gamma-glutamyl-gamma- aminobutyrate [H]

COG id: COG2071

COG function: function code R; Predicted glutamine amidotransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87081871, Length=260, Percent_Identity=36.9230769230769, Blast_Score=136, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR011697 [H]

Pfam domain/function: PF07722 Peptidase_C26 [H]

EC number: =3.5.1.94 [H]

Molecular weight: Translated: 29225; Mature: 29093

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGRAVIGVIGNAHLVDNRFAVQHVGERNLRAVAEVAGALPLMFGGSSEFAQIEDLLEVV
CCCCEEEEEECCCEEECCHHHHHHCCCHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHH
DGILLTGGRANVHPTYFKTEPNPRHEPYDQPRDELALALVRTCVERGVPLFGICRGFQEM
HHHEEECCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
NVAYGGSLHPEIRELPGRMNHRMPRLENGEIHPDPTVVFADRHDVQLTSDGVFARLLGRD
HHHCCCCCCHHHHHCCCHHHHCCCCCCCCCCCCCCEEEEECCCCEEECCCCHHHHHHHHH
LIRVNSLHGQGILEPGPRIVIEGVAEDGTIEAIRIADAPGFALGVQWHAEYDPQVNPINR
HHEEECCCCCCCCCCCCEEEEEECCCCCCEEEEEEECCCCEEEEEEEECCCCCCCCHHHH
ALFVAFGEAVSAYRTARQRPAALRRSG
HHHHHHHHHHHHHHHHHHCCHHHHCCC
>Mature Secondary Structure 
TGRAVIGVIGNAHLVDNRFAVQHVGERNLRAVAEVAGALPLMFGGSSEFAQIEDLLEVV
CCCEEEEEECCCEEECCHHHHHHCCCHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHH
DGILLTGGRANVHPTYFKTEPNPRHEPYDQPRDELALALVRTCVERGVPLFGICRGFQEM
HHHEEECCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
NVAYGGSLHPEIRELPGRMNHRMPRLENGEIHPDPTVVFADRHDVQLTSDGVFARLLGRD
HHHCCCCCCHHHHHCCCHHHHCCCCCCCCCCCCCCEEEEECCCCEEECCCCHHHHHHHHH
LIRVNSLHGQGILEPGPRIVIEGVAEDGTIEAIRIADAPGFALGVQWHAEYDPQVNPINR
HHEEECCCCCCCCCCCCEEEEEECCCCCCEEEEEEECCCCEEEEEEEECCCCCCCCHHHH
ALFVAFGEAVSAYRTARQRPAALRRSG
HHHHHHHHHHHHHHHHHHCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]