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The map label for this gene is menE [H]

Identifier: 192289845

GI number: 192289845

Start: 1515193

End: 1516704

Strand: Reverse

Name: menE [H]

Synonym: Rpal_1435

Alternate gene names: 192289845

Gene position: 1516704-1515193 (Counterclockwise)

Preceding gene: 192289851

Following gene: 192289841

Centisome position: 26.4

GC content: 66.2

Gene sequence:

>1512_bases
ATGATGGCGCTTCACGACTTCACGCTAGCCGACGTCTATCGCCGCAACGCGGCGCTGTTTCCCGATCGCACGGCTTTCGT
GGTCGATGGCGTCCGCCTCACCCACCGCGACTATCTGGCACGGGCCGAGCGGCTGGCCAGCGGCCTGCTCCGCGACAGCG
TGCACACCGGCGACCGGGTGGCGATTCTGTCGCAGAATTGCATCGAGATGATCGAGCTGATCGGCGCGGTGGCGTTGATC
GGCGCGATCCTGCTGCCCGTGAACTATCGGCTCAACGCCGACGAGATCGCCTTCGTGCTCGGCGACGGCGCCCCCAGCGT
GGTGGTGGCCGGCACCGATTATCGCGACATCGTTGCCGGGGTGCTGCCTTCGCTCGGCGGCGTCAAGAAAGCCTACGCGA
TCGGCGACGGTAGCGGACCGTTTGCGCCGTTCAAGGATCTCGCCTCGGACACGCCGTTCAGCGCCCCGGAGTTCGGCGCT
GCCGACGGCTTCGTCATCATCCACACCGCCGCGGTCGGCGGCCGGCCGCGCGGCGCGCTGATCTCGCAGGGCAATCTCTT
GATCGCGCAAAGCTCGCTGGTCGACGCATGGCGACTGACCGAAGCCGACGTCAATCTCGGCATGCTACCGCTGTTTCACG
TCACCGGGCTCGGCCTGATGCTGACCCTGCAACAGGCCGGCGGTGCGAGTGTGATCGCCGCGAAGTTCGACCCAGCGCAG
GCCGCACGCGACATCGAAGCTCACAAGGTGACGGTGATGGCCGAGTTCGCGCCGATGCTCGGCAACATTCTCGATCAGGC
CGAGCCGGCGCAGCTGGCCAGCCTGCGCGCCGTGACCGGGCTCGACACGCCGGAGACGATCGAGCGGTTCGAGGCCACCT
GCCCCAATGCGACGTTCTGGGCAACGTTCGGCCAATCAGAAACCTCGGGGCTCTCAACTTTTGCGCCCTATCGTGACCGG
CCGAAATCCGCCGGGCGCCCCCTGTTCTGGCGCACCGTCGCGGTGGTCGATGCCGAAGATCGCCCGCTGCCGCCGGGCGA
AGTCGGCGAGATCGTGCTGCGCGGCCCGACCGTATTCAAAGGCTACTGGAACAATGCGGCCGCCACCCAGCATGCGTTCC
GCAATGGTTGGCACCATACCGGGGACATGGGCCGATTCGATGCCGACGGCTATTTGTTCTACGCCGGCCGCGCGCCCGAA
AAGGAGCTGATCAAGACCGGCGGCGAGAACGTCTATCCGGCCGAGGTCGAAGGCGCACTGAAGCAGCATCCAGCGATCGC
CGACGCGGTGGTGATCGGCGTGCCAGATCCGCAATGGAGCGAAGCGATCAAGGCGGTGTGCGTATGCAAACCAGGCGAAA
GCATCGCCGCCGACGCCCTCGCCGAATTCGTCGCATCGCTGATCGCGCGCTACAAGAAGCCAAAGCACGTGGTGTTCGTC
GAGGAGCTGCCGAAGGATGCGAAGGGTGCTATCGATCGGGCCGCAGTGAAGACAGCGCACGGGCAGGCCTGA

Upstream 100 bases:

>100_bases
GATGTTCGCTACAAAGCAAGGCGCGAACGGCCGGCTGAACCGGCCGGCGCAACAATCCAGGCCGACCGCACCATGCGACC
TCGGCTGTACCAGGGGGGAC

Downstream 100 bases:

>100_bases
CGCCGGCCTGCGCGCTCACTCACTGCAGCGAAAACCGCACCGGCACGGTGAAGCTGAGCGACGATTGGGTGATCTCGCTC
GGGAACGGCGGCAGTGGCTG

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MMALHDFTLADVYRRNAALFPDRTAFVVDGVRLTHRDYLARAERLASGLLRDSVHTGDRVAILSQNCIEMIELIGAVALI
GAILLPVNYRLNADEIAFVLGDGAPSVVVAGTDYRDIVAGVLPSLGGVKKAYAIGDGSGPFAPFKDLASDTPFSAPEFGA
ADGFVIIHTAAVGGRPRGALISQGNLLIAQSSLVDAWRLTEADVNLGMLPLFHVTGLGLMLTLQQAGGASVIAAKFDPAQ
AARDIEAHKVTVMAEFAPMLGNILDQAEPAQLASLRAVTGLDTPETIERFEATCPNATFWATFGQSETSGLSTFAPYRDR
PKSAGRPLFWRTVAVVDAEDRPLPPGEVGEIVLRGPTVFKGYWNNAAATQHAFRNGWHHTGDMGRFDADGYLFYAGRAPE
KELIKTGGENVYPAEVEGALKQHPAIADAVVIGVPDPQWSEAIKAVCVCKPGESIAADALAEFVASLIARYKKPKHVVFV
EELPKDAKGAIDRAAVKTAHGQA

Sequences:

>Translated_503_residues
MMALHDFTLADVYRRNAALFPDRTAFVVDGVRLTHRDYLARAERLASGLLRDSVHTGDRVAILSQNCIEMIELIGAVALI
GAILLPVNYRLNADEIAFVLGDGAPSVVVAGTDYRDIVAGVLPSLGGVKKAYAIGDGSGPFAPFKDLASDTPFSAPEFGA
ADGFVIIHTAAVGGRPRGALISQGNLLIAQSSLVDAWRLTEADVNLGMLPLFHVTGLGLMLTLQQAGGASVIAAKFDPAQ
AARDIEAHKVTVMAEFAPMLGNILDQAEPAQLASLRAVTGLDTPETIERFEATCPNATFWATFGQSETSGLSTFAPYRDR
PKSAGRPLFWRTVAVVDAEDRPLPPGEVGEIVLRGPTVFKGYWNNAAATQHAFRNGWHHTGDMGRFDADGYLFYAGRAPE
KELIKTGGENVYPAEVEGALKQHPAIADAVVIGVPDPQWSEAIKAVCVCKPGESIAADALAEFVASLIARYKKPKHVVFV
EELPKDAKGAIDRAAVKTAHGQA
>Mature_503_residues
MMALHDFTLADVYRRNAALFPDRTAFVVDGVRLTHRDYLARAERLASGLLRDSVHTGDRVAILSQNCIEMIELIGAVALI
GAILLPVNYRLNADEIAFVLGDGAPSVVVAGTDYRDIVAGVLPSLGGVKKAYAIGDGSGPFAPFKDLASDTPFSAPEFGA
ADGFVIIHTAAVGGRPRGALISQGNLLIAQSSLVDAWRLTEADVNLGMLPLFHVTGLGLMLTLQQAGGASVIAAKFDPAQ
AARDIEAHKVTVMAEFAPMLGNILDQAEPAQLASLRAVTGLDTPETIERFEATCPNATFWATFGQSETSGLSTFAPYRDR
PKSAGRPLFWRTVAVVDAEDRPLPPGEVGEIVLRGPTVFKGYWNNAAATQHAFRNGWHHTGDMGRFDADGYLFYAGRAPE
KELIKTGGENVYPAEVEGALKQHPAIADAVVIGVPDPQWSEAIKAVCVCKPGESIAADALAEFVASLIARYKKPKHVVFV
EELPKDAKGAIDRAAVKTAHGQA

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=542, Percent_Identity=29.1512915129151, Blast_Score=178, Evalue=1e-44,
Organism=Homo sapiens, GI187761343, Length=542, Percent_Identity=29.1512915129151, Blast_Score=178, Evalue=1e-44,
Organism=Homo sapiens, GI156151445, Length=543, Percent_Identity=25.7826887661142, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI122937307, Length=494, Percent_Identity=26.5182186234818, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI58082049, Length=479, Percent_Identity=27.9749478079332, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI157311624, Length=493, Percent_Identity=27.789046653144, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI157311622, Length=493, Percent_Identity=27.789046653144, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI42544132, Length=478, Percent_Identity=24.4769874476987, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI38505220, Length=487, Percent_Identity=25.2566735112936, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI115511026, Length=503, Percent_Identity=25.4473161033797, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI28416953, Length=525, Percent_Identity=25.5238095238095, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI27477105, Length=181, Percent_Identity=39.7790055248619, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI13375727, Length=544, Percent_Identity=21.875, Blast_Score=76, Evalue=7e-14,
Organism=Escherichia coli, GI221142682, Length=510, Percent_Identity=29.6078431372549, Blast_Score=192, Evalue=6e-50,
Organism=Escherichia coli, GI145693145, Length=518, Percent_Identity=28.1853281853282, Blast_Score=169, Evalue=5e-43,
Organism=Escherichia coli, GI1788107, Length=531, Percent_Identity=25.6120527306968, Blast_Score=159, Evalue=4e-40,
Organism=Escherichia coli, GI1786810, Length=524, Percent_Identity=27.0992366412214, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI1790505, Length=533, Percent_Identity=24.953095684803, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788595, Length=502, Percent_Identity=24.5019920318725, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1786801, Length=449, Percent_Identity=22.71714922049, Blast_Score=70, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17558820, Length=497, Percent_Identity=28.7726358148893, Blast_Score=175, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI71994703, Length=545, Percent_Identity=25.3211009174312, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71994694, Length=545, Percent_Identity=25.3211009174312, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI71994690, Length=545, Percent_Identity=25.3211009174312, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI32563687, Length=496, Percent_Identity=25.8064516129032, Blast_Score=141, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI17559526, Length=354, Percent_Identity=30.225988700565, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17531443, Length=435, Percent_Identity=26.8965517241379, Blast_Score=134, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI17557194, Length=528, Percent_Identity=24.8106060606061, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17560308, Length=559, Percent_Identity=24.865831842576, Blast_Score=124, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI17560140, Length=514, Percent_Identity=24.9027237354086, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17538037, Length=504, Percent_Identity=24.8015873015873, Blast_Score=118, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI71996755, Length=510, Percent_Identity=24.1176470588235, Blast_Score=102, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI71985884, Length=204, Percent_Identity=32.843137254902, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6319699, Length=360, Percent_Identity=30.5555555555556, Blast_Score=178, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6319264, Length=552, Percent_Identity=23.1884057971014, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6323182, Length=553, Percent_Identity=24.0506329113924, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21355181, Length=500, Percent_Identity=27.6, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI18859661, Length=364, Percent_Identity=30.4945054945055, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24581924, Length=534, Percent_Identity=25.2808988764045, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24648253, Length=515, Percent_Identity=24.6601941747573, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24648255, Length=515, Percent_Identity=24.6601941747573, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24656500, Length=527, Percent_Identity=25.6166982922201, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24653035, Length=512, Percent_Identity=23.828125, Blast_Score=118, Evalue=9e-27,
Organism=Drosophila melanogaster, GI21356947, Length=505, Percent_Identity=24.1584158415842, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI161076582, Length=515, Percent_Identity=24.2718446601942, Blast_Score=115, Evalue=1e-25,
Organism=Drosophila melanogaster, GI281365686, Length=353, Percent_Identity=26.0623229461756, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI19922652, Length=523, Percent_Identity=24.6653919694073, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24648257, Length=515, Percent_Identity=25.0485436893204, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21356441, Length=501, Percent_Identity=23.75249500998, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24648260, Length=487, Percent_Identity=24.0246406570842, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI20130357, Length=483, Percent_Identity=23.8095238095238, Blast_Score=106, Evalue=5e-23,
Organism=Drosophila melanogaster, GI21358303, Length=501, Percent_Identity=22.9540918163673, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24583465, Length=516, Percent_Identity=23.8372093023256, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24583463, Length=516, Percent_Identity=23.8372093023256, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI281364798, Length=516, Percent_Identity=23.8372093023256, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI19921316, Length=554, Percent_Identity=24.0072202166065, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI62472339, Length=532, Percent_Identity=23.4962406015038, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24667955, Length=565, Percent_Identity=23.3628318584071, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24667959, Length=388, Percent_Identity=23.9690721649485, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17933690, Length=153, Percent_Identity=31.3725490196078, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 53544; Mature: 53544

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMALHDFTLADVYRRNAALFPDRTAFVVDGVRLTHRDYLARAERLASGLLRDSVHTGDRV
CCEECCHHHHHHHHCCCCCCCCCCEEEECCEEEHHHHHHHHHHHHHHHHHHHHCCCCCEE
AILSQNCIEMIELIGAVALIGAILLPVNYRLNADEIAFVLGDGAPSVVVAGTDYRDIVAG
EEHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCEEEEEECCCCEEEEECCCHHHHHHH
VLPSLGGVKKAYAIGDGSGPFAPFKDLASDTPFSAPEFGAADGFVIIHTAAVGGRPRGAL
HHHCCCCCCEEEEECCCCCCCCCHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCE
ISQGNLLIAQSSLVDAWRLTEADVNLGMLPLFHVTGLGLMLTLQQAGGASVIAAKFDPAQ
EECCCEEEEEHHHHHHHHEECCCCCCCCHHHHHHHCCCEEEEEECCCCCEEEEEECCHHH
AARDIEAHKVTVMAEFAPMLGNILDQAEPAQLASLRAVTGLDTPETIERFEATCPNATFW
HHHCCHHHEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEE
ATFGQSETSGLSTFAPYRDRPKSAGRPLFWRTVAVVDAEDRPLPPGEVGEIVLRGPTVFK
EECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCEEEECCEEEE
GYWNNAAATQHAFRNGWHHTGDMGRFDADGYLFYAGRAPEKELIKTGGENVYPAEVEGAL
CCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHCCCCCCCCHHHHHHH
KQHPAIADAVVIGVPDPQWSEAIKAVCVCKPGESIAADALAEFVASLIARYKKPKHVVFV
HHCCCCCCEEEEECCCCCHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEH
EELPKDAKGAIDRAAVKTAHGQA
HHCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MMALHDFTLADVYRRNAALFPDRTAFVVDGVRLTHRDYLARAERLASGLLRDSVHTGDRV
CCEECCHHHHHHHHCCCCCCCCCCEEEECCEEEHHHHHHHHHHHHHHHHHHHHCCCCCEE
AILSQNCIEMIELIGAVALIGAILLPVNYRLNADEIAFVLGDGAPSVVVAGTDYRDIVAG
EEHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCEEEEEECCCCEEEEECCCHHHHHHH
VLPSLGGVKKAYAIGDGSGPFAPFKDLASDTPFSAPEFGAADGFVIIHTAAVGGRPRGAL
HHHCCCCCCEEEEECCCCCCCCCHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCE
ISQGNLLIAQSSLVDAWRLTEADVNLGMLPLFHVTGLGLMLTLQQAGGASVIAAKFDPAQ
EECCCEEEEEHHHHHHHHEECCCCCCCCHHHHHHHCCCEEEEEECCCCCEEEEEECCHHH
AARDIEAHKVTVMAEFAPMLGNILDQAEPAQLASLRAVTGLDTPETIERFEATCPNATFW
HHHCCHHHEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEE
ATFGQSETSGLSTFAPYRDRPKSAGRPLFWRTVAVVDAEDRPLPPGEVGEIVLRGPTVFK
EECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCEEEECCEEEE
GYWNNAAATQHAFRNGWHHTGDMGRFDADGYLFYAGRAPEKELIKTGGENVYPAEVEGAL
CCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHCCCCCCCCHHHHHHH
KQHPAIADAVVIGVPDPQWSEAIKAVCVCKPGESIAADALAEFVASLIARYKKPKHVVFV
HHCCCCCCEEEEECCCCCHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEH
EELPKDAKGAIDRAAVKTAHGQA
HHCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA