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The map label for this gene is menB [C]

Identifier: 192289841

GI number: 192289841

Start: 1511889

End: 1512737

Strand: Reverse

Name: menB [C]

Synonym: Rpal_1431

Alternate gene names: 192289841

Gene position: 1512737-1511889 (Counterclockwise)

Preceding gene: 192289845

Following gene: 192289840

Centisome position: 26.34

GC content: 63.96

Gene sequence:

>849_bases
ATGAGCTCCTACGAGACGATCCTGGTCGAGCGACCGGATCCGGCGGTCGCACGAATCGTGATGAACCGTCCCGACGCGCG
CAACGCGCAAAATCTGCAGATGACCTACGACCTCAACGCCGCGTTCGACGACGCGGTGCAGGACGACAGCGTCAAGGTGA
TTATCCTGGCCGGTGCCGGTCCGCACTTCTCCGCCGGCCACGATCTGCGCGCGACCACCAAGAATGAAGCCGGTATCGAC
TTTCCGCCGGTCGGCGCCTGGGGCGGGTTTCGCGAGCCGGGCGCGCACGGACGGATGGCGCGCGAGCAGGAGATCTATCT
GCAGATCACCCGGCGGTGGCGCAATCTCGCCAAGCCGACCATCGCCGAGGTGCACGGCAAGTGCATCGCCGGCGGGCTGA
TGCTGGCCTGGGCCTGCGATCTGATCATCGCCGCCGACTCGGCCGAGTTCTGCGATCCGGTGGTGACAATGGGCGTGTGC
GGCGTCGAGTGGTTCGTGCATCCCTGGGAGCTCGGCCCACGCAAGGCCAAGGAGTTGCTCTTCACCGCCGACAGCTGGAG
CGCGCAAGAAGCCCATCAGCTCGGCATGGTCAATCACGTGGTGCCTGCCACCGAACTATCCGCTTTCGCCATGGCGCTTG
CGCAACGTATCGCCGCCAAGCCGAGCTTTGCACTGAAGATGACCAAGGAAGCGGTCAACCGCTCGGTGGATATCCAGGGG
CAGCCGGCCGCGATCGATCAGGCGTTCGCGCTGCATCAGCTTTGTCACGCTCACAATCTGCAGGAGTTTGGAATGATCGT
CGATCCGTCGGGACTGCATCCGTCGGTGCGCAAGCAAGTAAAGGTGTAA

Upstream 100 bases:

>100_bases
GAGCCGCAGAGATGCCGTTTGCAGTCGCGCGCGCGACCTGCCAAACTCCGCGAAACACAACCGGCGCCACAAGCCGAGAC
AATAAGAAACGGGAGGATGG

Downstream 100 bases:

>100_bases
GCGATGGATCTCACGCCCAGTGACGAACAACGGCTGCTGCGCGAAAGCGCCGACCGCTTCATCGCCGAGACCTACACGAC
GAAGCTGCGCGAGCAGAACG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MSSYETILVERPDPAVARIVMNRPDARNAQNLQMTYDLNAAFDDAVQDDSVKVIILAGAGPHFSAGHDLRATTKNEAGID
FPPVGAWGGFREPGAHGRMAREQEIYLQITRRWRNLAKPTIAEVHGKCIAGGLMLAWACDLIIAADSAEFCDPVVTMGVC
GVEWFVHPWELGPRKAKELLFTADSWSAQEAHQLGMVNHVVPATELSAFAMALAQRIAAKPSFALKMTKEAVNRSVDIQG
QPAAIDQAFALHQLCHAHNLQEFGMIVDPSGLHPSVRKQVKV

Sequences:

>Translated_282_residues
MSSYETILVERPDPAVARIVMNRPDARNAQNLQMTYDLNAAFDDAVQDDSVKVIILAGAGPHFSAGHDLRATTKNEAGID
FPPVGAWGGFREPGAHGRMAREQEIYLQITRRWRNLAKPTIAEVHGKCIAGGLMLAWACDLIIAADSAEFCDPVVTMGVC
GVEWFVHPWELGPRKAKELLFTADSWSAQEAHQLGMVNHVVPATELSAFAMALAQRIAAKPSFALKMTKEAVNRSVDIQG
QPAAIDQAFALHQLCHAHNLQEFGMIVDPSGLHPSVRKQVKV
>Mature_281_residues
SSYETILVERPDPAVARIVMNRPDARNAQNLQMTYDLNAAFDDAVQDDSVKVIILAGAGPHFSAGHDLRATTKNEAGIDF
PPVGAWGGFREPGAHGRMAREQEIYLQITRRWRNLAKPTIAEVHGKCIAGGLMLAWACDLIIAADSAEFCDPVVTMGVCG
VEWFVHPWELGPRKAKELLFTADSWSAQEAHQLGMVNHVVPATELSAFAMALAQRIAAKPSFALKMTKEAVNRSVDIQGQ
PAAIDQAFALHQLCHAHNLQEFGMIVDPSGLHPSVRKQVKV

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=216, Percent_Identity=32.8703703703704, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI194097323, Length=247, Percent_Identity=28.7449392712551, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI45643119, Length=235, Percent_Identity=28.0851063829787, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI260275230, Length=235, Percent_Identity=28.0851063829787, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI260274832, Length=235, Percent_Identity=28.0851063829787, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI70995211, Length=234, Percent_Identity=31.6239316239316, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1788597, Length=241, Percent_Identity=31.1203319502075, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1787660, Length=224, Percent_Identity=32.1428571428571, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI221142681, Length=222, Percent_Identity=34.2342342342342, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1787659, Length=242, Percent_Identity=27.6859504132231, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI87082183, Length=220, Percent_Identity=31.3636363636364, Blast_Score=82, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17554946, Length=241, Percent_Identity=30.2904564315353, Blast_Score=97, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI25145438, Length=223, Percent_Identity=29.5964125560538, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=218, Percent_Identity=27.9816513761468, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17540714, Length=267, Percent_Identity=26.9662921348315, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17549921, Length=249, Percent_Identity=26.5060240963855, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17536985, Length=236, Percent_Identity=27.1186440677966, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17560910, Length=241, Percent_Identity=26.1410788381743, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17540306, Length=209, Percent_Identity=28.2296650717703, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17534483, Length=242, Percent_Identity=26.4462809917355, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI20129971, Length=229, Percent_Identity=29.2576419213974, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24653477, Length=229, Percent_Identity=29.2576419213974, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI19920382, Length=247, Percent_Identity=29.9595141700405, Blast_Score=95, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24653139, Length=266, Percent_Identity=29.3233082706767, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21357171, Length=244, Percent_Identity=29.9180327868852, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24654903, Length=232, Percent_Identity=24.5689655172414, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 30795; Mature: 30663

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSYETILVERPDPAVARIVMNRPDARNAQNLQMTYDLNAAFDDAVQDDSVKVIILAGAG
CCCCCEEEEECCCHHHHHHEECCCCCCCCCCEEEEEECCCHHHCCCCCCCEEEEEEECCC
PHFSAGHDLRATTKNEAGIDFPPVGAWGGFREPGAHGRMAREQEIYLQITRRWRNLAKPT
CCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCH
IAEVHGKCIAGGLMLAWACDLIIAADSAEFCDPVVTMGVCGVEWFVHPWELGPRKAKELL
HHHHCCHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHE
FTADSWSAQEAHQLGMVNHVVPATELSAFAMALAQRIAAKPSFALKMTKEAVNRSVDIQG
EECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEECCCC
QPAAIDQAFALHQLCHAHNLQEFGMIVDPSGLHPSVRKQVKV
CCHHHHHHHHHHHHHHHCCHHHCCEEECCCCCCHHHHHHCCC
>Mature Secondary Structure 
SSYETILVERPDPAVARIVMNRPDARNAQNLQMTYDLNAAFDDAVQDDSVKVIILAGAG
CCCCEEEEECCCHHHHHHEECCCCCCCCCCEEEEEECCCHHHCCCCCCCEEEEEEECCC
PHFSAGHDLRATTKNEAGIDFPPVGAWGGFREPGAHGRMAREQEIYLQITRRWRNLAKPT
CCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCH
IAEVHGKCIAGGLMLAWACDLIIAADSAEFCDPVVTMGVCGVEWFVHPWELGPRKAKELL
HHHHCCHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHE
FTADSWSAQEAHQLGMVNHVVPATELSAFAMALAQRIAAKPSFALKMTKEAVNRSVDIQG
EECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEECCCC
QPAAIDQAFALHQLCHAHNLQEFGMIVDPSGLHPSVRKQVKV
CCHHHHHHHHHHHHHHHCCHHHCCEEECCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA