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The map label for this gene is pleD [H]

Identifier: 192289851

GI number: 192289851

Start: 1521062

End: 1521988

Strand: Reverse

Name: pleD [H]

Synonym: Rpal_1441

Alternate gene names: 192289851

Gene position: 1521988-1521062 (Counterclockwise)

Preceding gene: 192289852

Following gene: 192289845

Centisome position: 26.5

GC content: 67.1

Gene sequence:

>927_bases
ATGAACAATGACCTGAAGAAGCGCGATATCGCTCTGGTCGTCGACGACTCTCCGGAGACGTTGAGGATGCTGACCGACGC
CCTCGACGGCGCCGGCATGACCGTGATGGTCGCGCTCGACGGCGCCGCAGCGATGCGGATCGTCGAACAGATCACGCCCG
ACATCATCCTGCTCGATGCGGTGATGCCGGGCATGGACGGCTTCGAAACCTGCCGGCGGCTGAAGCGCAGCCCGGGACTA
TCCGACGTGCCGGTGATCTTCATGACCGGCCTGTCGGAGTCCGAGCACATCGTGCGCGGGCTGGAGGCCGGCGGCGTCGA
CTACGTCACCAAGCCGATTGCGATCGTGGAGATGCTGGCGCGGATTCGCGTGCATCTGAACAATGCCCGCGTCAGCCAGA
GCGCGCGCGCTGCGCTGGATGTGTCGGGACGTTTCCTGCTCGCGGTCGACCGGCTCGGCAAGGTGCTGTGGGCGACACCG
CAGGCGCAGAAGCTGCTCGCCGCCGACGGCGCGCCGGAGGACGGCCTGCGGCTCGACTTGCCGAAGCCGTGGCTCGAATG
GCTGGAGCAGGTGCACACCGGCCTCGCCCAGCCGAAGGTGCCGATGAAGTCCTCGAGGCTGCGCAACGACGAGCTGCGGC
TGCAATACATGGGCAAGCTCGGCCCCAATGAATTCCTGCTGCGGCTGGGACGCGACACCTCGGCCGAAGCGCCGACGCAG
TTCTCCGCCGAGTTCGGCCTCACCAGCCGCGAAAGCGAAGTGCTGGCGTGGCTCAGCAAGGGCAAGACCAACCGCGACAT
CGCGCAGATCCTGGGGCTCAGCCCGCGCACCGTCGACAAGCACCTCGAGCAGATCTACGCCAAGCTCGGCGTCGAAAACC
GCACCGCCGCCGCGGCGATCGCCACCAATGCGAGCCGGCGCGACTAA

Upstream 100 bases:

>100_bases
GATTATCCGGAGACCTGGGCGTTCGTGTCGCAGATGCGAACGATGGTGGAGCAGTTCGACCTCGATACCTACATGAAGAC
GCTGAACACGCTGTATCGCC

Downstream 100 bases:

>100_bases
CTGTTTGTCTCCGTGCACCCGAGCCGGCCCGCGCCTGCTTCGATCTCGGCATTGAGCTCGCCGCCGGCAAGCACGACGAT
CGCCGTCATCCACATCCAGA

Product: LuxR family transcriptional regulator

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MNNDLKKRDIALVVDDSPETLRMLTDALDGAGMTVMVALDGAAAMRIVEQITPDIILLDAVMPGMDGFETCRRLKRSPGL
SDVPVIFMTGLSESEHIVRGLEAGGVDYVTKPIAIVEMLARIRVHLNNARVSQSARAALDVSGRFLLAVDRLGKVLWATP
QAQKLLAADGAPEDGLRLDLPKPWLEWLEQVHTGLAQPKVPMKSSRLRNDELRLQYMGKLGPNEFLLRLGRDTSAEAPTQ
FSAEFGLTSRESEVLAWLSKGKTNRDIAQILGLSPRTVDKHLEQIYAKLGVENRTAAAAIATNASRRD

Sequences:

>Translated_308_residues
MNNDLKKRDIALVVDDSPETLRMLTDALDGAGMTVMVALDGAAAMRIVEQITPDIILLDAVMPGMDGFETCRRLKRSPGL
SDVPVIFMTGLSESEHIVRGLEAGGVDYVTKPIAIVEMLARIRVHLNNARVSQSARAALDVSGRFLLAVDRLGKVLWATP
QAQKLLAADGAPEDGLRLDLPKPWLEWLEQVHTGLAQPKVPMKSSRLRNDELRLQYMGKLGPNEFLLRLGRDTSAEAPTQ
FSAEFGLTSRESEVLAWLSKGKTNRDIAQILGLSPRTVDKHLEQIYAKLGVENRTAAAAIATNASRRD
>Mature_308_residues
MNNDLKKRDIALVVDDSPETLRMLTDALDGAGMTVMVALDGAAAMRIVEQITPDIILLDAVMPGMDGFETCRRLKRSPGL
SDVPVIFMTGLSESEHIVRGLEAGGVDYVTKPIAIVEMLARIRVHLNNARVSQSARAALDVSGRFLLAVDRLGKVLWATP
QAQKLLAADGAPEDGLRLDLPKPWLEWLEQVHTGLAQPKVPMKSSRLRNDELRLQYMGKLGPNEFLLRLGRDTSAEAPTQ
FSAEFGLTSRESEVLAWLSKGKTNRDIAQILGLSPRTVDKHLEQIYAKLGVENRTAAAAIATNASRRD

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1788394, Length=161, Percent_Identity=33.5403726708075, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1786599, Length=112, Percent_Identity=38.3928571428571, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790299, Length=131, Percent_Identity=35.8778625954198, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786784, Length=115, Percent_Identity=35.6521739130435, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788550, Length=107, Percent_Identity=35.5140186915888, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI87082012, Length=132, Percent_Identity=31.0606060606061, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1786911, Length=115, Percent_Identity=38.2608695652174, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790860, Length=125, Percent_Identity=33.6, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI145693157, Length=104, Percent_Identity=37.5, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787229, Length=112, Percent_Identity=34.8214285714286, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1789809, Length=140, Percent_Identity=32.1428571428571, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1790863, Length=152, Percent_Identity=28.9473684210526, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 33725; Mature: 33725

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNDLKKRDIALVVDDSPETLRMLTDALDGAGMTVMVALDGAAAMRIVEQITPDIILLDA
CCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHCCCEEEEEH
VMPGMDGFETCRRLKRSPGLSDVPVIFMTGLSESEHIVRGLEAGGVDYVTKPIAIVEMLA
HCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHH
RIRVHLNNARVSQSARAALDVSGRFLLAVDRLGKVLWATPQAQKLLAADGAPEDGLRLDL
HHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHEECCCHHHHHHHCCCCCCCCCEECC
PKPWLEWLEQVHTGLAQPKVPMKSSRLRNDELRLQYMGKLGPNEFLLRLGRDTSAEAPTQ
CHHHHHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCC
FSAEFGLTSRESEVLAWLSKGKTNRDIAQILGLSPRTVDKHLEQIYAKLGVENRTAAAAI
CHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHH
ATNASRRD
HCCCCCCC
>Mature Secondary Structure
MNNDLKKRDIALVVDDSPETLRMLTDALDGAGMTVMVALDGAAAMRIVEQITPDIILLDA
CCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHCCCEEEEEH
VMPGMDGFETCRRLKRSPGLSDVPVIFMTGLSESEHIVRGLEAGGVDYVTKPIAIVEMLA
HCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHH
RIRVHLNNARVSQSARAALDVSGRFLLAVDRLGKVLWATPQAQKLLAADGAPEDGLRLDL
HHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHEECCCHHHHHHHCCCCCCCCCEECC
PKPWLEWLEQVHTGLAQPKVPMKSSRLRNDELRLQYMGKLGPNEFLLRLGRDTSAEAPTQ
CHHHHHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCC
FSAEFGLTSRESEVLAWLSKGKTNRDIAQILGLSPRTVDKHLEQIYAKLGVENRTAAAAI
CHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHH
ATNASRRD
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA