Click here to switch to the map view.

The map label for this gene is paaG [C]

Identifier: 192288915

GI number: 192288915

Start: 523686

End: 524471

Strand: Reverse

Name: paaG [C]

Synonym: Rpal_0485

Alternate gene names: 192288915

Gene position: 524471-523686 (Counterclockwise)

Preceding gene: 192288916

Following gene: 192288914

Centisome position: 9.13

GC content: 66.92

Gene sequence:

>786_bases
ATGACCGACATCGTCCTGCAAGATCTCGACCGCGGCCTGCTCACCATCACGATGAACCGTCCGGATCGGCGCAACGCGCT
CAATCAGGACATGATCCGCGGCTTGGTGGAGGCGGCGCATCGCGCCAAGGACGATCCGGAAGTCCGCGCGGTGTTGTTGA
AGGGCGGCGGTACATTCTGCGTCGGCGGCGACGTCAAGTCGATGGCGGCTGCGGGTGCTCCGCCGCCGCTGGAACAGAAG
GTGGCGAACCTCCGCCGCGGCATGGAAGTGTCACGTCTGCTGCATCAGATGCCCAAGCCGGTGGTGGCGCAGATCGACGG
CGCAGCCGCTGGCGCCGGGCTGTCGATGGCGCTCGCCTGCGATCTGCGGGTCGCAGGTGCTTCGGCCAAGATCACCACTG
CGTTCGCCAAGGTCGGCCTCTCCGGCGATTTCGGCGGCACGTACTTCCTGACTCAGCTGATCGGCGCCGCGAAGGCGCGG
GAACTATATCTCACCTCGCCGGTGCTGACCGCCGAGCAGGCGCAGGCGCTCGGCATCGTCAGCCGCGTGGTGCCCGATGC
CGAGGTCGAATCCGCCACGCGCGAGCTGGCGCTGTCGTTGGCACAGGGCCCGACCGTGACGCTCGGATACATCAAGACCA
ACATCAACAACGCCGAAACCCTGTCGCTCGAAGCCTGCTTCGACGCCGAGGCGCTACATCACTCGCGCTGCGCTGAGACT
GCCGACCACAAGGAAGCCGCTGCCGCCTTCGTCGAGAAGCGCGCGCCGGTGTTCCAGGGCCGCTGA

Upstream 100 bases:

>100_bases
CCTCGCAACGACAGAACGCTCATGGTTCGAGACGGCGCTGGCGCCTCCTCACCATGGGTGCCCATGCCTGGAGCGCAACG
AATAACAACAAGGACCCGCC

Downstream 100 bases:

>100_bases
CGATGGCGCCGTTCCTTCGCCTGCGCCAGATCTGCCTCGTCACTGAGCCGATCGAGCCCGCCGCCTCGCGCATTGCGACG
ATCATGGGACTCAACATCTG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MTDIVLQDLDRGLLTITMNRPDRRNALNQDMIRGLVEAAHRAKDDPEVRAVLLKGGGTFCVGGDVKSMAAAGAPPPLEQK
VANLRRGMEVSRLLHQMPKPVVAQIDGAAAGAGLSMALACDLRVAGASAKITTAFAKVGLSGDFGGTYFLTQLIGAAKAR
ELYLTSPVLTAEQAQALGIVSRVVPDAEVESATRELALSLAQGPTVTLGYIKTNINNAETLSLEACFDAEALHHSRCAET
ADHKEAAAAFVEKRAPVFQGR

Sequences:

>Translated_261_residues
MTDIVLQDLDRGLLTITMNRPDRRNALNQDMIRGLVEAAHRAKDDPEVRAVLLKGGGTFCVGGDVKSMAAAGAPPPLEQK
VANLRRGMEVSRLLHQMPKPVVAQIDGAAAGAGLSMALACDLRVAGASAKITTAFAKVGLSGDFGGTYFLTQLIGAAKAR
ELYLTSPVLTAEQAQALGIVSRVVPDAEVESATRELALSLAQGPTVTLGYIKTNINNAETLSLEACFDAEALHHSRCAET
ADHKEAAAAFVEKRAPVFQGR
>Mature_260_residues
TDIVLQDLDRGLLTITMNRPDRRNALNQDMIRGLVEAAHRAKDDPEVRAVLLKGGGTFCVGGDVKSMAAAGAPPPLEQKV
ANLRRGMEVSRLLHQMPKPVVAQIDGAAAGAGLSMALACDLRVAGASAKITTAFAKVGLSGDFGGTYFLTQLIGAAKARE
LYLTSPVLTAEQAQALGIVSRVVPDAEVESATRELALSLAQGPTVTLGYIKTNINNAETLSLEACFDAEALHHSRCAETA
DHKEAAAAFVEKRAPVFQGR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI4502327, Length=268, Percent_Identity=32.4626865671642, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI194097323, Length=246, Percent_Identity=31.3008130081301, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI70995211, Length=188, Percent_Identity=32.9787234042553, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI31542718, Length=256, Percent_Identity=25.78125, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI260274832, Length=220, Percent_Identity=28.1818181818182, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI45643119, Length=220, Percent_Identity=28.1818181818182, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI260275230, Length=220, Percent_Identity=28.1818181818182, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI213417737, Length=246, Percent_Identity=26.4227642276423, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI157694516, Length=246, Percent_Identity=26.4227642276423, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI37594471, Length=219, Percent_Identity=28.7671232876712, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI37594469, Length=219, Percent_Identity=28.7671232876712, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI68989263, Length=171, Percent_Identity=31.5789473684211, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI25453481, Length=185, Percent_Identity=29.7297297297297, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI51538573, Length=185, Percent_Identity=29.7297297297297, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI4757966, Length=185, Percent_Identity=29.7297297297297, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI51538581, Length=185, Percent_Identity=29.7297297297297, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI4757968, Length=185, Percent_Identity=29.1891891891892, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI49355787, Length=185, Percent_Identity=29.1891891891892, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1787660, Length=264, Percent_Identity=37.5, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1787659, Length=263, Percent_Identity=32.319391634981, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1788597, Length=262, Percent_Identity=29.7709923664122, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI221142681, Length=255, Percent_Identity=30.1960784313725, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI87082183, Length=258, Percent_Identity=25.1937984496124, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1788682, Length=188, Percent_Identity=31.9148936170213, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1790281, Length=182, Percent_Identity=27.4725274725275, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17540714, Length=210, Percent_Identity=33.8095238095238, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI25145438, Length=244, Percent_Identity=28.6885245901639, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17554946, Length=254, Percent_Identity=29.1338582677165, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17535521, Length=255, Percent_Identity=27.4509803921569, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17560910, Length=200, Percent_Identity=29, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17536985, Length=218, Percent_Identity=27.0642201834862, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17534483, Length=185, Percent_Identity=28.6486486486486, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17549921, Length=196, Percent_Identity=27.5510204081633, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25144157, Length=187, Percent_Identity=29.4117647058824, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25144160, Length=187, Percent_Identity=29.4117647058824, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17558304, Length=166, Percent_Identity=31.9277108433735, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=201, Percent_Identity=25.3731343283582, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24653139, Length=269, Percent_Identity=32.7137546468402, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI20129971, Length=244, Percent_Identity=30.327868852459, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24653477, Length=244, Percent_Identity=30.327868852459, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI21357171, Length=236, Percent_Identity=31.3559322033898, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19922422, Length=267, Percent_Identity=26.5917602996255, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24650670, Length=205, Percent_Identity=30.2439024390244, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28571730, Length=142, Percent_Identity=32.3943661971831, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI28571729, Length=142, Percent_Identity=32.3943661971831, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24654903, Length=201, Percent_Identity=28.3582089552239, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19920382, Length=188, Percent_Identity=27.1276595744681, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27486; Mature: 27355

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDIVLQDLDRGLLTITMNRPDRRNALNQDMIRGLVEAAHRAKDDPEVRAVLLKGGGTFC
CCCHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEE
VGGDVKSMAAAGAPPPLEQKVANLRRGMEVSRLLHQMPKPVVAQIDGAAAGAGLSMALAC
ECCCHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCEEECCCCCCCCCCEEEEEE
DLRVAGASAKITTAFAKVGLSGDFGGTYFLTQLIGAAKARELYLTSPVLTAEQAQALGIV
EEEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCEEEECCCCCCHHHHHHHHHH
SRVVPDAEVESATRELALSLAQGPTVTLGYIKTNINNAETLSLEACFDAEALHHSRCAET
HHHCCCHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEEEHHHHCHHHHHHHHHHHH
ADHKEAAAAFVEKRAPVFQGR
HHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TDIVLQDLDRGLLTITMNRPDRRNALNQDMIRGLVEAAHRAKDDPEVRAVLLKGGGTFC
CCHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEE
VGGDVKSMAAAGAPPPLEQKVANLRRGMEVSRLLHQMPKPVVAQIDGAAAGAGLSMALAC
ECCCHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCEEECCCCCCCCCCEEEEEE
DLRVAGASAKITTAFAKVGLSGDFGGTYFLTQLIGAAKARELYLTSPVLTAEQAQALGIV
EEEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCEEEECCCCCCHHHHHHHHHH
SRVVPDAEVESATRELALSLAQGPTVTLGYIKTNINNAETLSLEACFDAEALHHSRCAET
HHHCCCHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEEEHHHHCHHHHHHHHHHHH
ADHKEAAAAFVEKRAPVFQGR
HHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA