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The map label for this gene is miaB [H]

Identifier: 192288883

GI number: 192288883

Start: 487356

End: 488747

Strand: Reverse

Name: miaB [H]

Synonym: Rpal_0452

Alternate gene names: 192288883

Gene position: 488747-487356 (Counterclockwise)

Preceding gene: 192288884

Following gene: 192288882

Centisome position: 8.51

GC content: 65.37

Gene sequence:

>1392_bases
ATGTCTCCGCCGCGCAAGCTGCACATCAAGTCATACGGCTGTCAGATGAATGTCTACGATGCTCAGCGCATGGTGGATGC
GCTGGCGCCGGAAGGCTTTGTCGAGACCGCGAACGTGGATGACGCCGACCTGGTGATCCTGAACACCTGCCACATCCGCG
AAAAAGCGTCCGAGAAGGTCTATTCCGAGCTCGGCCGGCTGCGCGTCGCGCGCGACGAAGCCGCAAACCATGGCCGGCGG
ATGCAGATCGCGGTCGCCGGATGCGTAGCGCAGGCGGAGGGCGAGGAAATCATCCGCCGCGCGCCAGTGGTCGACGTCGT
GGTCGGGCCGCAGAGCTATCACAACCTGCCGCAATTGCTGGCAAAGGCCGAGCAGCACGGCCGAGCGCTGGAGACCGAGT
TCCCGATCGAGGACAAGTTTGGCGTGTTGCCGCAGCCGGCGCCGGACGCGATCCGTGCCCGCGGCATTTCGGCGTTCGTG
ACTGTCCAGGAAGGCTGCGACAAATTCTGCACCTTCTGCGTCGTGCCCTATACCCGCGGCGCCGAGATGTCGCGCCCGGT
GGTGGCGATCGTCGAGGACGTCAAACGGCTCGCCGAGAACGGGGTCCGCGAGGTCACGCTGATCGGCCAGAACGTCAATG
CCTATCACGGCGACGGGCCGGACGGGCGCGCCTGGTCGCTCGGGCGCCTGGTGCGGCGCCTTGCCGAAATTCCCGGTATC
GTCCGGCTGCGCTACTCGACCAGCCATCCAAACGACGTCGACGATGATCTCTTGGCCGCGCACCGCGATCTGCCGGCGCT
GATGCCGTTCGTGCATCTGCCGGTGCAGTCCGGCTCGGACCGGATCTTGGCGGCGATGAACCGCAAGCACACCGCCGACG
ATTATCGCCGCGTGATCGACCGGTTCCGGTCTGCCTCCGAGACGATCGCATTCTCGTCCGACTTCATCGTCGGTTTCCCG
GGTGAAACCGAGCGCGATTTTTCCGCAACTCTCGCGCTTGTCGCACAAATCGGATACGCTGGGGCGTATTCGTTCAAATA
CTCCCCGCGGCCCGGCACGCCCGCGGCGGACATGGTGGAGATGGTGCCTGCAGCGGTCATGGACGAGCGGTTGGAGCAGC
TCCAGCAATTGATCGACCAGCAGCAATCGGCCTTCAACAAGGCTGCGATCGGCCGGACCGTGGAGGTCCTGTTCGAGCGC
GCCGGCCGCAAGCCCGGTCAGATCGTCGGCCGCACCGCTTATCTGCAGCCCGCCCATGTGATGGCGCCCGACAGCATCAT
CGGAAAGGTGCTGCCGGTCCGCGTCGATAGCCTCGAACGCTACAGTCTGCTTGGCGAACTCGCATCCGCAACCTCCCGGC
CGGCCGATGCGATGGCCGCCACAGGAGCCTGA

Upstream 100 bases:

>100_bases
CGGCAAATCGCCGGTCGCGGCTGGATTTCGTCGGGTTTCGCCTATAAGTGACGGTAACGCGCTGGAACACGCGCGTTTTT
TGTTTGGGCTCCCGCACACG

Downstream 100 bases:

>100_bases
GCCCCCTTGGTCAAAAGCGCACCGGAATCGTCTTCATTCGTTTCCCGCCGCAAACCCGATCGCGACAGCTTGAACCCGCC
TGAAACCCAGGTCGTCATCG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MSPPRKLHIKSYGCQMNVYDAQRMVDALAPEGFVETANVDDADLVILNTCHIREKASEKVYSELGRLRVARDEAANHGRR
MQIAVAGCVAQAEGEEIIRRAPVVDVVVGPQSYHNLPQLLAKAEQHGRALETEFPIEDKFGVLPQPAPDAIRARGISAFV
TVQEGCDKFCTFCVVPYTRGAEMSRPVVAIVEDVKRLAENGVREVTLIGQNVNAYHGDGPDGRAWSLGRLVRRLAEIPGI
VRLRYSTSHPNDVDDDLLAAHRDLPALMPFVHLPVQSGSDRILAAMNRKHTADDYRRVIDRFRSASETIAFSSDFIVGFP
GETERDFSATLALVAQIGYAGAYSFKYSPRPGTPAADMVEMVPAAVMDERLEQLQQLIDQQQSAFNKAAIGRTVEVLFER
AGRKPGQIVGRTAYLQPAHVMAPDSIIGKVLPVRVDSLERYSLLGELASATSRPADAMAATGA

Sequences:

>Translated_463_residues
MSPPRKLHIKSYGCQMNVYDAQRMVDALAPEGFVETANVDDADLVILNTCHIREKASEKVYSELGRLRVARDEAANHGRR
MQIAVAGCVAQAEGEEIIRRAPVVDVVVGPQSYHNLPQLLAKAEQHGRALETEFPIEDKFGVLPQPAPDAIRARGISAFV
TVQEGCDKFCTFCVVPYTRGAEMSRPVVAIVEDVKRLAENGVREVTLIGQNVNAYHGDGPDGRAWSLGRLVRRLAEIPGI
VRLRYSTSHPNDVDDDLLAAHRDLPALMPFVHLPVQSGSDRILAAMNRKHTADDYRRVIDRFRSASETIAFSSDFIVGFP
GETERDFSATLALVAQIGYAGAYSFKYSPRPGTPAADMVEMVPAAVMDERLEQLQQLIDQQQSAFNKAAIGRTVEVLFER
AGRKPGQIVGRTAYLQPAHVMAPDSIIGKVLPVRVDSLERYSLLGELASATSRPADAMAATGA
>Mature_462_residues
SPPRKLHIKSYGCQMNVYDAQRMVDALAPEGFVETANVDDADLVILNTCHIREKASEKVYSELGRLRVARDEAANHGRRM
QIAVAGCVAQAEGEEIIRRAPVVDVVVGPQSYHNLPQLLAKAEQHGRALETEFPIEDKFGVLPQPAPDAIRARGISAFVT
VQEGCDKFCTFCVVPYTRGAEMSRPVVAIVEDVKRLAENGVREVTLIGQNVNAYHGDGPDGRAWSLGRLVRRLAEIPGIV
RLRYSTSHPNDVDDDLLAAHRDLPALMPFVHLPVQSGSDRILAAMNRKHTADDYRRVIDRFRSASETIAFSSDFIVGFPG
ETERDFSATLALVAQIGYAGAYSFKYSPRPGTPAADMVEMVPAAVMDERLEQLQQLIDQQQSAFNKAAIGRTVEVLFERA
GRKPGQIVGRTAYLQPAHVMAPDSIIGKVLPVRVDSLERYSLLGELASATSRPADAMAATGA

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872782, Length=431, Percent_Identity=33.4106728538283, Blast_Score=269, Evalue=3e-72,
Organism=Homo sapiens, GI28872784, Length=431, Percent_Identity=33.4106728538283, Blast_Score=269, Evalue=5e-72,
Organism=Homo sapiens, GI93277076, Length=463, Percent_Identity=28.5097192224622, Blast_Score=177, Evalue=2e-44,
Organism=Escherichia coli, GI1786882, Length=446, Percent_Identity=45.5156950672646, Blast_Score=395, Evalue=1e-111,
Organism=Escherichia coli, GI1787057, Length=465, Percent_Identity=29.0322580645161, Blast_Score=178, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI17553146, Length=419, Percent_Identity=32.4582338902148, Blast_Score=243, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI71996771, Length=382, Percent_Identity=25.9162303664921, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI21356207, Length=432, Percent_Identity=32.6388888888889, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI19922432, Length=458, Percent_Identity=27.5109170305677, Blast_Score=170, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 50652; Mature: 50521

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPPRKLHIKSYGCQMNVYDAQRMVDALAPEGFVETANVDDADLVILNTCHIREKASEKV
CCCCCEEEEEECCCEEEHHHHHHHHHHHCCCCCEEECCCCCCCEEEEEEHHHHHHHHHHH
YSELGRLRVARDEAANHGRRMQIAVAGCVAQAEGEEIIRRAPVVDVVVGPQSYHNLPQLL
HHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHCCHHHHHHHCCEEEEEECCHHHHHHHHHH
AKAEQHGRALETEFPIEDKFGVLPQPAPDAIRARGISAFVTVQEGCDKFCTFCVVPYTRG
HHHHHCCCEECCCCCCCCCCCCCCCCCCHHHHHCCCEEEEEHHHHHHHHHHEEECCCCCC
AEMSRPVVAIVEDVKRLAENGVREVTLIGQNVNAYHGDGPDGRAWSLGRLVRRLAEIPGI
CCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCE
VRLRYSTSHPNDVDDDLLAAHRDLPALMPFVHLPVQSGSDRILAAMNRKHTADDYRRVID
EEEEECCCCCCCCCHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHCCCCCHHHHHHHHH
RFRSASETIAFSSDFIVGFPGETERDFSATLALVAQIGYAGAYSFKYSPRPGTPAADMVE
HHHCCCCEEEECCCEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHH
MVPAAVMDERLEQLQQLIDQQQSAFNKAAIGRTVEVLFERAGRKPGQIVGRTAYLQPAHV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCEEEECCCHH
MAPDSIIGKVLPVRVDSLERYSLLGELASATSRPADAMAATGA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCC
>Mature Secondary Structure 
SPPRKLHIKSYGCQMNVYDAQRMVDALAPEGFVETANVDDADLVILNTCHIREKASEKV
CCCCEEEEEECCCEEEHHHHHHHHHHHCCCCCEEECCCCCCCEEEEEEHHHHHHHHHHH
YSELGRLRVARDEAANHGRRMQIAVAGCVAQAEGEEIIRRAPVVDVVVGPQSYHNLPQLL
HHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHCCHHHHHHHCCEEEEEECCHHHHHHHHHH
AKAEQHGRALETEFPIEDKFGVLPQPAPDAIRARGISAFVTVQEGCDKFCTFCVVPYTRG
HHHHHCCCEECCCCCCCCCCCCCCCCCCHHHHHCCCEEEEEHHHHHHHHHHEEECCCCCC
AEMSRPVVAIVEDVKRLAENGVREVTLIGQNVNAYHGDGPDGRAWSLGRLVRRLAEIPGI
CCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCE
VRLRYSTSHPNDVDDDLLAAHRDLPALMPFVHLPVQSGSDRILAAMNRKHTADDYRRVID
EEEEECCCCCCCCCHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHCCCCCHHHHHHHHH
RFRSASETIAFSSDFIVGFPGETERDFSATLALVAQIGYAGAYSFKYSPRPGTPAADMVE
HHHCCCCEEEECCCEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHH
MVPAAVMDERLEQLQQLIDQQQSAFNKAAIGRTVEVLFERAGRKPGQIVGRTAYLQPAHV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCEEEECCCHH
MAPDSIIGKVLPVRVDSLERYSLLGELASATSRPADAMAATGA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA