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The map label for this gene is nusA [H]

Identifier: 192288873

GI number: 192288873

Start: 476678

End: 478291

Strand: Reverse

Name: nusA [H]

Synonym: Rpal_0442

Alternate gene names: 192288873

Gene position: 478291-476678 (Counterclockwise)

Preceding gene: 192288874

Following gene: 192288872

Centisome position: 8.33

GC content: 65.37

Gene sequence:

>1614_bases
ATGGCCGTCAGCGCCAATAGGCTGGAACTGCTGCAGATCGCCGATGCGGTGGCTCGCGAGAAAACCATCGACCGCAGCAT
CGTGATTGCGGCGATGGAAGATGCGATCGCCAAGGCGGCGCGCGCCCGCTACGGCTCGGAGACCGACGTCCATGCTGAGA
TCGACCCGAAGAAGGGCGAGCTGCGGCTGTCGCGCCACATGCTGGTGGTCGAGCAGGTCGAAAACCCCGCCAACCAGATT
TCGCTGAAGGACGCGCAGCGCGCCAATCCCGGCGCGCAGATCGGCGACACCATCGCCGACACCCTGCCGCCGCTGGAATA
CGGCCGTATCGCCGCGCAGTCGGCCAAGCAGGTGATCGTGCAGAAGGTCCGTGAGGCCGAGCGCGACCGCCAGTACATGG
AATTCAAGGACCGGATCGGCGACATCGTCAACGGCGTCGTCAAGCGCGTCGAATACGGCAGCGTGATCGTCGACCTTGGC
CGCGGCGAAGCGATCATCCGCCGCGACGAGATGCTGCCGCGTGAGTCGTTCCGCAACGGCGACCGCGTCCGCGCCTATGT
GTTCGACGTCCGCCGCGAGACCCGCGGCCCGCAGATCTTCCTGTCGCGCACCCATCCGCAGTTCATGGCCAAGCTGTTCG
CGCAGGAAGTGCCGGAAATCTACGACGGCATCGTCGAGATCAAGGCGGTCGCCCGCGATCCCGGCTCGCGCGCCAAGATC
GGCGTCGTCTCGCGGGACTCCTCGGTCGATCCGGTCGGCGCCTGCGTCGGTATGCGCGGTTCGCGCGTGCAGGCGGTGGT
GAACGAACTGCAGGGCGAGAAGATCGACATCATTCCGTGGTCGCCGGACATCGCCACCTTCGTGGTCAACGCGCTGGCGC
CGGCCGAAGTCTCGAAGGTCGTGATCGACGAAGATCGCGAACGCATCGAGGTTGTGGTTCCGGATACCAATAACCAACTA
TCCCTGGCGATTGGTCGCCGCGGTCAGAACGTGCGGCTCGCTTCGCAGCTCACCGGCTGGGACATCGACATCCTGACCGA
GAGCGAGGAATCCGAGCGCCGCCAAGCCGACTTCGAGAAGACCACCCGGGCCTTCATGGACGCGCTGAACGTCGACGAGG
TCGTCGGCCAGCTGCTCGCCTCCGAAGGTTTCACCTCGGTCGAAGAACTGGCGCTGGTCGACCCGCGCGAACTCGCCTCG
ATCGAAGGTTTCGACGAGGAAACCGCCGCCGAACTGCAGACCCGCGCCAGCGAATATCTCGACCGGATTGAATCCGAGCT
CGAGGCCCGGCGCCTGGAGCTCGGCGTCGAAGATGCTCTGAAGGACGTTCCCGGCGTCACCTCGAAGATGCTGGTCAAGT
TCGGCGAGAACGACGTCAAGACCGTCGAGGATCTGGCCGGCTGCGCCACCGACGATCTGGTGGGTTGGACCGAGCGCAAG
GACGGCGCCGAGCCGGTGAAGTATCCTGGCATTCTCGACGGCATGGAGATGTCGCGCGAGGACGCCGAACACCTGATCAT
GCAGGCCCGCGTCAAGGCCGGCTGGATCGACGAGTCGGAGCTCGCCTCCGAAGAAGAACCCGCGGACGAAGCGTCCGACG
AGTCGGCGGACTGA

Upstream 100 bases:

>100_bases
AGGCGCCGACCAAGCCGAAACCGCAGAACACCAAGCAACACCGTCTCGCCGCCGACAAAGCGCGGCGCGGCGAGATCGAC
ACCTCTGAGGGAGACTGACC

Downstream 100 bases:

>100_bases
ACCGCCGATGCGTCGCGCCCCGAATCCGCGCAGCACGTGAGATGAAAGGCCTGCTGCCCCCATGCTTGCTGCCGAAACCG
GCGCAGATCTCGACGACGGA

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 537; Mature: 536

Protein sequence:

>537_residues
MAVSANRLELLQIADAVAREKTIDRSIVIAAMEDAIAKAARARYGSETDVHAEIDPKKGELRLSRHMLVVEQVENPANQI
SLKDAQRANPGAQIGDTIADTLPPLEYGRIAAQSAKQVIVQKVREAERDRQYMEFKDRIGDIVNGVVKRVEYGSVIVDLG
RGEAIIRRDEMLPRESFRNGDRVRAYVFDVRRETRGPQIFLSRTHPQFMAKLFAQEVPEIYDGIVEIKAVARDPGSRAKI
GVVSRDSSVDPVGACVGMRGSRVQAVVNELQGEKIDIIPWSPDIATFVVNALAPAEVSKVVIDEDRERIEVVVPDTNNQL
SLAIGRRGQNVRLASQLTGWDIDILTESEESERRQADFEKTTRAFMDALNVDEVVGQLLASEGFTSVEELALVDPRELAS
IEGFDEETAAELQTRASEYLDRIESELEARRLELGVEDALKDVPGVTSKMLVKFGENDVKTVEDLAGCATDDLVGWTERK
DGAEPVKYPGILDGMEMSREDAEHLIMQARVKAGWIDESELASEEEPADEASDESAD

Sequences:

>Translated_537_residues
MAVSANRLELLQIADAVAREKTIDRSIVIAAMEDAIAKAARARYGSETDVHAEIDPKKGELRLSRHMLVVEQVENPANQI
SLKDAQRANPGAQIGDTIADTLPPLEYGRIAAQSAKQVIVQKVREAERDRQYMEFKDRIGDIVNGVVKRVEYGSVIVDLG
RGEAIIRRDEMLPRESFRNGDRVRAYVFDVRRETRGPQIFLSRTHPQFMAKLFAQEVPEIYDGIVEIKAVARDPGSRAKI
GVVSRDSSVDPVGACVGMRGSRVQAVVNELQGEKIDIIPWSPDIATFVVNALAPAEVSKVVIDEDRERIEVVVPDTNNQL
SLAIGRRGQNVRLASQLTGWDIDILTESEESERRQADFEKTTRAFMDALNVDEVVGQLLASEGFTSVEELALVDPRELAS
IEGFDEETAAELQTRASEYLDRIESELEARRLELGVEDALKDVPGVTSKMLVKFGENDVKTVEDLAGCATDDLVGWTERK
DGAEPVKYPGILDGMEMSREDAEHLIMQARVKAGWIDESELASEEEPADEASDESAD
>Mature_536_residues
AVSANRLELLQIADAVAREKTIDRSIVIAAMEDAIAKAARARYGSETDVHAEIDPKKGELRLSRHMLVVEQVENPANQIS
LKDAQRANPGAQIGDTIADTLPPLEYGRIAAQSAKQVIVQKVREAERDRQYMEFKDRIGDIVNGVVKRVEYGSVIVDLGR
GEAIIRRDEMLPRESFRNGDRVRAYVFDVRRETRGPQIFLSRTHPQFMAKLFAQEVPEIYDGIVEIKAVARDPGSRAKIG
VVSRDSSVDPVGACVGMRGSRVQAVVNELQGEKIDIIPWSPDIATFVVNALAPAEVSKVVIDEDRERIEVVVPDTNNQLS
LAIGRRGQNVRLASQLTGWDIDILTESEESERRQADFEKTTRAFMDALNVDEVVGQLLASEGFTSVEELALVDPRELASI
EGFDEETAAELQTRASEYLDRIESELEARRLELGVEDALKDVPGVTSKMLVKFGENDVKTVEDLAGCATDDLVGWTERKD
GAEPVKYPGILDGMEMSREDAEHLIMQARVKAGWIDESELASEEEPADEASDESAD

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=479, Percent_Identity=44.4676409185804, Blast_Score=389, Evalue=1e-109,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 59408; Mature: 59277

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVSANRLELLQIADAVAREKTIDRSIVIAAMEDAIAKAARARYGSETDVHAEIDPKKGE
CCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCC
LRLSRHMLVVEQVENPANQISLKDAQRANPGAQIGDTIADTLPPLEYGRIAAQSAKQVIV
HHHHHHHHHHHHHCCCHHHEECCHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
QKVREAERDRQYMEFKDRIGDIVNGVVKRVEYGSVIVDLGRGEAIIRRDEMLPRESFRNG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEHHHCCCHHHHCCC
DRVRAYVFDVRRETRGPQIFLSRTHPQFMAKLFAQEVPEIYDGIVEIKAVARDPGSRAKI
CCEEEHHEEHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
GVVSRDSSVDPVGACVGMRGSRVQAVVNELQGEKIDIIPWSPDIATFVVNALAPAEVSKV
EEEECCCCCCHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCHHHHHHH
VIDEDRERIEVVVPDTNNQLSLAIGRRGQNVRLASQLTGWDIDILTESEESERRQADFEK
HHCCCCCEEEEEECCCCCEEEEEECCCCCCEEEHHHCCCCEEEEEECCCHHHHHHHHHHH
TTRAFMDALNVDEVVGQLLASEGFTSVEELALVDPRELASIEGFDEETAAELQTRASEYL
HHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHH
DRIESELEARRLELGVEDALKDVPGVTSKMLVKFGENDVKTVEDLAGCATDDLVGWTERK
HHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHCCCCHHHCCCCCCC
DGAEPVKYPGILDGMEMSREDAEHLIMQARVKAGWIDESELASEEEPADEASDESAD
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AVSANRLELLQIADAVAREKTIDRSIVIAAMEDAIAKAARARYGSETDVHAEIDPKKGE
CCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCC
LRLSRHMLVVEQVENPANQISLKDAQRANPGAQIGDTIADTLPPLEYGRIAAQSAKQVIV
HHHHHHHHHHHHHCCCHHHEECCHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
QKVREAERDRQYMEFKDRIGDIVNGVVKRVEYGSVIVDLGRGEAIIRRDEMLPRESFRNG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEHHHCCCHHHHCCC
DRVRAYVFDVRRETRGPQIFLSRTHPQFMAKLFAQEVPEIYDGIVEIKAVARDPGSRAKI
CCEEEHHEEHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
GVVSRDSSVDPVGACVGMRGSRVQAVVNELQGEKIDIIPWSPDIATFVVNALAPAEVSKV
EEEECCCCCCHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCHHHHHHH
VIDEDRERIEVVVPDTNNQLSLAIGRRGQNVRLASQLTGWDIDILTESEESERRQADFEK
HHCCCCCEEEEEECCCCCEEEEEECCCCCCEEEHHHCCCCEEEEEECCCHHHHHHHHHHH
TTRAFMDALNVDEVVGQLLASEGFTSVEELALVDPRELASIEGFDEETAAELQTRASEYL
HHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHH
DRIESELEARRLELGVEDALKDVPGVTSKMLVKFGENDVKTVEDLAGCATDDLVGWTERK
HHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHCCCCHHHCCCCCCC
DGAEPVKYPGILDGMEMSREDAEHLIMQARVKAGWIDESELASEEEPADEASDESAD
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]