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The map label for this gene is dgoD [H]

Identifier: 190894753

GI number: 190894753

Start: 444674

End: 445861

Strand: Reverse

Name: dgoD [H]

Synonym: RHECIAT_PC0000418

Alternate gene names: 190894753

Gene position: 445861-444674 (Counterclockwise)

Preceding gene: 190894754

Following gene: 190894745

Centisome position: 40.85

GC content: 63.05

Gene sequence:

>1188_bases
ATGAAGATCGACCGCATGCGGGTTTTCATGACCCGCGACAAGGACCGTCCGCGTGTAATCGTCGCGCTCGATACCGATGA
TGGGCTCACCGGCTGGGGCGAGTGCTACAATCATGGCCCCGACAAGGCGCTGCCGCCGCTGCTCGATTACCTCTACGGGT
TCATCTCAGGGCAGGATCCGACGCGCGTCGAATATCTGGTCAACCTGCTGATCCAGCAAAGCCGCTTCCCGCCGGGCGCG
CTCGGCCTTGCGGCAATCTCCGCGCTCGATCACTGCCTGTGGGATCTGGCGGCCAAGGCGGCGAACGTGCCGGTTTACAA
GCTGCTCGGCGGCGAGGTGCGCGATCGCATCAAGGTCTATGCCGGGGTCTACACCGCGCCCGATGCACCGGCCGCCCGCG
ACGAGTTCGACCGCCTGAAGGAGGGTTGGGGTTTCACCGCCTTCAAGCTCAGTCCCTGGCGCATCGATATGCATTCCAAT
CGCTGGGGCAATGTCGTGAAGGCGTCCGCGGATTATTTCCGCTCGCTGCGCGAGACAGTCAGCGACGAGTACGAGATCGC
CTTCGACGCCCATGCCAAGATTTTTGAGCCGATCGCCGCAAGGCAGCTCGGCAATGCGCTGGCGCCTTACGATCCCCTGT
TTTTCGAGGAGCCGCTGCGTCCGGAGAATATCGAGGCCTGGGGTGACCTGAAACAGGGCCTCAACTGCGTGCTGGCAACC
GGCGAGTCGCTCTACAACAGAAACGAATTCCTGCGTCTGCTGCAGGTCAAGGGCGCCGATCTGATCCAGCCCGATATCTG
CGTCGTCGGCGGCATCAGCGAGATGCGCCGCATCGCCACCCTTGCCGAAGCCTACTTCGTCGGTGTCGCTCCGCACAATC
CGATGGGGCCGCTCGCGACTGCCGTCAACGTGCATTTCTCGGCGGCCGCGCAGAATTTCCGTATCCTTGAATACCGGCTG
CCGAAGGGCCAAGCCTATGTTTATGGCGGCCTTGATGTCGAGACGCGCCAGGGAGAGACCCGTTACGTCGTCGACCCCTA
TCTGCCGAAGGACGGTTATCTCGAACTGCGTCCCGACCGGCCCGGCTGGGGCGTCGAAATGGACGAGAAGGCGATGGAGG
CGGAAGGCTACATCCACTGGCAGAGGCGCGTGCCGAAGCGGCCAGACGGCTCCTACGCCTTCGCTTGA

Upstream 100 bases:

>100_bases
AGCCGCCGGCGCCTACAATCCCGGAACCGATAACGAGCATGTCTACGTGCAGACGCGCGTGGCACGCCGCAGCTATTTCA
ATCGCTAGAAGGACAGACTG

Downstream 100 bases:

>100_bases
AGACGTTGCTCGAAAATCAGGAACTGGCTGCTGCCGCATGGAAGCGGCCAGTTTTTTATGCTCCGATGCATGTCGCCCGG
AAGTGTGCAGCGGTTCCGGG

Product: putative racemase

Products: NA

Alternate protein names: GalD [H]

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MKIDRMRVFMTRDKDRPRVIVALDTDDGLTGWGECYNHGPDKALPPLLDYLYGFISGQDPTRVEYLVNLLIQQSRFPPGA
LGLAAISALDHCLWDLAAKAANVPVYKLLGGEVRDRIKVYAGVYTAPDAPAARDEFDRLKEGWGFTAFKLSPWRIDMHSN
RWGNVVKASADYFRSLRETVSDEYEIAFDAHAKIFEPIAARQLGNALAPYDPLFFEEPLRPENIEAWGDLKQGLNCVLAT
GESLYNRNEFLRLLQVKGADLIQPDICVVGGISEMRRIATLAEAYFVGVAPHNPMGPLATAVNVHFSAAAQNFRILEYRL
PKGQAYVYGGLDVETRQGETRYVVDPYLPKDGYLELRPDRPGWGVEMDEKAMEAEGYIHWQRRVPKRPDGSYAFA

Sequences:

>Translated_395_residues
MKIDRMRVFMTRDKDRPRVIVALDTDDGLTGWGECYNHGPDKALPPLLDYLYGFISGQDPTRVEYLVNLLIQQSRFPPGA
LGLAAISALDHCLWDLAAKAANVPVYKLLGGEVRDRIKVYAGVYTAPDAPAARDEFDRLKEGWGFTAFKLSPWRIDMHSN
RWGNVVKASADYFRSLRETVSDEYEIAFDAHAKIFEPIAARQLGNALAPYDPLFFEEPLRPENIEAWGDLKQGLNCVLAT
GESLYNRNEFLRLLQVKGADLIQPDICVVGGISEMRRIATLAEAYFVGVAPHNPMGPLATAVNVHFSAAAQNFRILEYRL
PKGQAYVYGGLDVETRQGETRYVVDPYLPKDGYLELRPDRPGWGVEMDEKAMEAEGYIHWQRRVPKRPDGSYAFA
>Mature_395_residues
MKIDRMRVFMTRDKDRPRVIVALDTDDGLTGWGECYNHGPDKALPPLLDYLYGFISGQDPTRVEYLVNLLIQQSRFPPGA
LGLAAISALDHCLWDLAAKAANVPVYKLLGGEVRDRIKVYAGVYTAPDAPAARDEFDRLKEGWGFTAFKLSPWRIDMHSN
RWGNVVKASADYFRSLRETVSDEYEIAFDAHAKIFEPIAARQLGNALAPYDPLFFEEPLRPENIEAWGDLKQGLNCVLAT
GESLYNRNEFLRLLQVKGADLIQPDICVVGGISEMRRIATLAEAYFVGVAPHNPMGPLATAVNVHFSAAAQNFRILEYRL
PKGQAYVYGGLDVETRQGETRYVVDPYLPKDGYLELRPDRPGWGVEMDEKAMEAEGYIHWQRRVPKRPDGSYAFA

Specific function: Catalyzes the dehydration of D-galactonate to 2-keto-3- deoxy-D-galactonate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GalD subfamily [H]

Homologues:

Organism=Homo sapiens, GI42544119, Length=357, Percent_Identity=23.8095238095238, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI186972148, Length=357, Percent_Identity=23.8095238095238, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI48994953, Length=400, Percent_Identity=32, Blast_Score=181, Evalue=6e-47,
Organism=Escherichia coli, GI1787864, Length=398, Percent_Identity=28.1407035175879, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI226510960, Length=371, Percent_Identity=25.3369272237197, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.6 [H]

Molecular weight: Translated: 44448; Mature: 44448

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIDRMRVFMTRDKDRPRVIVALDTDDGLTGWGECYNHGPDKALPPLLDYLYGFISGQDP
CCCCCEEEEEECCCCCCEEEEEEECCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHCCCCC
TRVEYLVNLLIQQSRFPPGALGLAAISALDHCLWDLAAKAANVPVYKLLGGEVRDRIKVY
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCHHHHHHEEEE
AGVYTAPDAPAARDEFDRLKEGWGFTAFKLSPWRIDMHSNRWGNVVKASADYFRSLRETV
EEEEECCCCCCHHHHHHHHHHCCCEEEEEECCEEEEECCCCCCCEEEHHHHHHHHHHHHH
SDEYEIAFDAHAKIFEPIAARQLGNALAPYDPLFFEEPLRPENIEAWGDLKQGLNCVLAT
CCCEEEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCEEEEEC
GESLYNRNEFLRLLQVKGADLIQPDICVVGGISEMRRIATLAEAYFVGVAPHNPMGPLAT
CHHHHHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHEEECCCCCCCCCHHH
AVNVHFSAAAQNFRILEYRLPKGQAYVYGGLDVETRQGETRYVVDPYLPKDGYLELRPDR
HEEEEEHHHCCCEEEEEEECCCCCEEEEECCEEECCCCCEEEEECCCCCCCCCEEECCCC
PGWGVEMDEKAMEAEGYIHWQRRVPKRPDGSYAFA
CCCCCCCCHHHHHCCCEEEEHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKIDRMRVFMTRDKDRPRVIVALDTDDGLTGWGECYNHGPDKALPPLLDYLYGFISGQDP
CCCCCEEEEEECCCCCCEEEEEEECCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHCCCCC
TRVEYLVNLLIQQSRFPPGALGLAAISALDHCLWDLAAKAANVPVYKLLGGEVRDRIKVY
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCHHHHHHEEEE
AGVYTAPDAPAARDEFDRLKEGWGFTAFKLSPWRIDMHSNRWGNVVKASADYFRSLRETV
EEEEECCCCCCHHHHHHHHHHCCCEEEEEECCEEEEECCCCCCCEEEHHHHHHHHHHHHH
SDEYEIAFDAHAKIFEPIAARQLGNALAPYDPLFFEEPLRPENIEAWGDLKQGLNCVLAT
CCCEEEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCEEEEEC
GESLYNRNEFLRLLQVKGADLIQPDICVVGGISEMRRIATLAEAYFVGVAPHNPMGPLAT
CHHHHHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHEEECCCCCCCCCHHH
AVNVHFSAAAQNFRILEYRLPKGQAYVYGGLDVETRQGETRYVVDPYLPKDGYLELRPDR
HEEEEEHHHCCCEEEEEEECCCCCEEEEECCEEECCCCCEEEEECCCCCCCCCEEECCCC
PGWGVEMDEKAMEAEGYIHWQRRVPKRPDGSYAFA
CCCCCCCCHHHHHCCCEEEEHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]