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The map label for this gene is dgoD1 [H]

Identifier: 190894754

GI number: 190894754

Start: 445874

End: 447085

Strand: Reverse

Name: dgoD1 [H]

Synonym: RHECIAT_PC0000419

Alternate gene names: 190894754

Gene position: 447085-445874 (Counterclockwise)

Preceding gene: 190894755

Following gene: 190894753

Centisome position: 40.96

GC content: 64.27

Gene sequence:

>1212_bases
ATGAAAATCACCGGAATCCGAACGTTTCTCATGCATGTCGGCCAGCCCGACCCCTCCAATTGGGCTGCCGACCAGCGGTC
CGGCGATCGCACTTCCAAGCAGTTCGGCGGCACCCGAAACTGGCTCTTTCTGAAGATCGATACCGACGAAGGCATAACCG
GCATCGGCGAGTGCTCAGGCTGGCCGCGCGTTGTCGAAACGGCGATCCACGACCTTGCGCCGCTGCTCATTGGCGAGGAT
CCGGCACATATCGAGCGCCTGTGGCAGAAGATGCATATCGCGATGATGGGTCACGGCATGCTCGGAACCGTCGGAGGCGG
GGCTATGACCGGCATCGACATGGCCCTCTGGGACATCAAGGGCAAGGCGCTCGGCGTGCCTGTCTGGATGCTGCTCGGCG
GCAAGGTGCGGGACCGGATCCCGATCTATTCGCATGCCAATACGCCCGAGCGGGCGCTTGCGCTCAAGGAGCGTGGCATC
AGGGCGATCAAATGCGGTGGCGTCGCCGATCCCGTTCGCAAGGTTGCAGCGCTGCGCGATGCCGTCGGCGACGACATGGA
TATTGCCATCGACCTGCATGGGCCGCCTTGGCTGACGCCAGCGGATGCCTGCCGGCTGGTGCGCGCGCTCGAACCCTACG
AGCTGATGTGGGTGGAAGACCCGATCGCCCCCGAGAATATCGAAGGCTACCGGCGCATCCGCGACGCCGCCCATGTGCCG
CTTGCGGCGGGCGAGCGTTCGGCAACCATTTTCGGCGAACGCGAGCTGATCGAGAGGGAACTGGTCGACGTGATTCAGCC
CGATACGGGCCGGGCCGGCGGCATTACCCAGATGAAGAAGATCGCGGCCATGGCTGAAGCCCATCATATCCAGATGGCGC
CGCATTCCGGCTCACTCGGACCGGTGGCGGAGTATGCGGCTCTGCATCTGCTGGCCGCCATCCCGAACGCTCTAATCCTC
GAGCGCCTCGACGATGACTGGGACGGCCGGCGCCAAACGATCGTGCCGCACCCTGAACAGGTCGACGGCTTGATCGCCGT
TCCCGATGGTCCGGGGCTGGGCTGCGATATCGACGAGGACTTCGTCGCCCGCTTCCCCAGCGCCGGCAATGTTTCGGTGC
CGCAAGCCGCCGGCGCCTACAATCCCGGAACCGATAACGAGCATGTCTACGTGCAGACGCGCGTGGCACGCCGCAGCTAT
TTCAATCGCTAG

Upstream 100 bases:

>100_bases
TGATCTCGGCAATCCCGCGCCCCGATCCGCGGGACCGCGATCATTCCAGGCGCTTCCGTTACGTCGCGCCCGACAGTCTG
AAGAATGGAAGTTCTCAGAG

Downstream 100 bases:

>100_bases
AAGGACAGACTGATGAAGATCGACCGCATGCGGGTTTTCATGACCCGCGACAAGGACCGTCCGCGTGTAATCGTCGCGCT
CGATACCGATGATGGGCTCA

Product: putative racemase

Products: NA

Alternate protein names: GalD 1 [H]

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MKITGIRTFLMHVGQPDPSNWAADQRSGDRTSKQFGGTRNWLFLKIDTDEGITGIGECSGWPRVVETAIHDLAPLLIGED
PAHIERLWQKMHIAMMGHGMLGTVGGGAMTGIDMALWDIKGKALGVPVWMLLGGKVRDRIPIYSHANTPERALALKERGI
RAIKCGGVADPVRKVAALRDAVGDDMDIAIDLHGPPWLTPADACRLVRALEPYELMWVEDPIAPENIEGYRRIRDAAHVP
LAAGERSATIFGERELIERELVDVIQPDTGRAGGITQMKKIAAMAEAHHIQMAPHSGSLGPVAEYAALHLLAAIPNALIL
ERLDDDWDGRRQTIVPHPEQVDGLIAVPDGPGLGCDIDEDFVARFPSAGNVSVPQAAGAYNPGTDNEHVYVQTRVARRSY
FNR

Sequences:

>Translated_403_residues
MKITGIRTFLMHVGQPDPSNWAADQRSGDRTSKQFGGTRNWLFLKIDTDEGITGIGECSGWPRVVETAIHDLAPLLIGED
PAHIERLWQKMHIAMMGHGMLGTVGGGAMTGIDMALWDIKGKALGVPVWMLLGGKVRDRIPIYSHANTPERALALKERGI
RAIKCGGVADPVRKVAALRDAVGDDMDIAIDLHGPPWLTPADACRLVRALEPYELMWVEDPIAPENIEGYRRIRDAAHVP
LAAGERSATIFGERELIERELVDVIQPDTGRAGGITQMKKIAAMAEAHHIQMAPHSGSLGPVAEYAALHLLAAIPNALIL
ERLDDDWDGRRQTIVPHPEQVDGLIAVPDGPGLGCDIDEDFVARFPSAGNVSVPQAAGAYNPGTDNEHVYVQTRVARRSY
FNR
>Mature_403_residues
MKITGIRTFLMHVGQPDPSNWAADQRSGDRTSKQFGGTRNWLFLKIDTDEGITGIGECSGWPRVVETAIHDLAPLLIGED
PAHIERLWQKMHIAMMGHGMLGTVGGGAMTGIDMALWDIKGKALGVPVWMLLGGKVRDRIPIYSHANTPERALALKERGI
RAIKCGGVADPVRKVAALRDAVGDDMDIAIDLHGPPWLTPADACRLVRALEPYELMWVEDPIAPENIEGYRRIRDAAHVP
LAAGERSATIFGERELIERELVDVIQPDTGRAGGITQMKKIAAMAEAHHIQMAPHSGSLGPVAEYAALHLLAAIPNALIL
ERLDDDWDGRRQTIVPHPEQVDGLIAVPDGPGLGCDIDEDFVARFPSAGNVSVPQAAGAYNPGTDNEHVYVQTRVARRSY
FNR

Specific function: Catalyzes the dehydration of D-galactonate to 2-keto-3- deoxy-D-galactonate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GalD subfamily [H]

Homologues:

Organism=Escherichia coli, GI48994953, Length=351, Percent_Identity=38.1766381766382, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1787864, Length=377, Percent_Identity=32.8912466843501, Blast_Score=171, Evalue=9e-44,
Organism=Escherichia coli, GI226510960, Length=366, Percent_Identity=27.0491803278689, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.6 [H]

Molecular weight: Translated: 43905; Mature: 43905

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITGIRTFLMHVGQPDPSNWAADQRSGDRTSKQFGGTRNWLFLKIDTDEGITGIGECSG
CCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCEEEEEEECCCCCCCCCCCCC
WPRVVETAIHDLAPLLIGEDPAHIERLWQKMHIAMMGHGMLGTVGGGAMTGIDMALWDIK
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHEEEEEECC
GKALGVPVWMLLGGKVRDRIPIYSHANTPERALALKERGIRAIKCGGVADPVRKVAALRD
CCEECCCEEEHCCCCHHCCCCEEECCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHH
AVGDDMDIAIDLHGPPWLTPADACRLVRALEPYELMWVEDPIAPENIEGYRRIRDAAHVP
HCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHCCC
LAAGERSATIFGERELIERELVDVIQPDTGRAGGITQMKKIAAMAEAHHIQMAPHSGSLG
EECCCCCEEECCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHEEEECCCCCCCC
PVAEYAALHLLAAIPNALILERLDDDWDGRRQTIVPHPEQVDGLIAVPDGPGLGCDIDED
HHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCHHCCCEEECCCCCCCCCCCCHH
FVARFPSAGNVSVPQAAGAYNPGTDNEHVYVQTRVARRSYFNR
HHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHCCC
>Mature Secondary Structure
MKITGIRTFLMHVGQPDPSNWAADQRSGDRTSKQFGGTRNWLFLKIDTDEGITGIGECSG
CCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCEEEEEEECCCCCCCCCCCCC
WPRVVETAIHDLAPLLIGEDPAHIERLWQKMHIAMMGHGMLGTVGGGAMTGIDMALWDIK
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHEEEEEECC
GKALGVPVWMLLGGKVRDRIPIYSHANTPERALALKERGIRAIKCGGVADPVRKVAALRD
CCEECCCEEEHCCCCHHCCCCEEECCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHH
AVGDDMDIAIDLHGPPWLTPADACRLVRALEPYELMWVEDPIAPENIEGYRRIRDAAHVP
HCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHCCC
LAAGERSATIFGERELIERELVDVIQPDTGRAGGITQMKKIAAMAEAHHIQMAPHSGSLG
EECCCCCEEECCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHEEEECCCCCCCC
PVAEYAALHLLAAIPNALILERLDDDWDGRRQTIVPHPEQVDGLIAVPDGPGLGCDIDED
HHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCHHCCCEEECCCCCCCCCCCCHH
FVARFPSAGNVSVPQAAGAYNPGTDNEHVYVQTRVARRSYFNR
HHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA