Click here to switch to the map view.

The map label for this gene is thuE [C]

Identifier: 190894738

GI number: 190894738

Start: 427868

End: 429133

Strand: Reverse

Name: thuE [C]

Synonym: RHECIAT_PC0000403

Alternate gene names: 190894738

Gene position: 429133-427868 (Counterclockwise)

Preceding gene: 190894740

Following gene: 190894737

Centisome position: 39.32

GC content: 58.93

Gene sequence:

>1266_bases
ATGAAGAAGTTTGCATTAAGCTTGTCGGTGCTCGGCGTCGCCATCGCCTTCGCTGGCCCGATCCGTGCCGCTGAAATTTC
GTTTGCCGCCAACACCACGGGAAAAAACGTCGAATTCCTCAACAAGCAACTGGCAATTTTCGAGAAGGAGACCGGCAATC
AGGTCAAACTCGTCACCATGCCGTCGTCGAGCAGCGAGCAGTTCTCCCAATATCGCCTGTGGCTTGCCGCCGGAAACAAG
GACATCGACGTCTATCAGACGGACGTCATCTGGGCGCCGCAGCTTGCTGACCAGTTCATCGATCTGAAGGACGCCACCAA
AGACGTGGCCGGCCAATTCTTCCCGTCGATCATCGCCTCGCAGACGGTCAACGGCCGGCTCGTGGCGCTGCCATTGTTTA
CCGATGCTCCGGCGCTGTTTTACCGGAAGGACCTGCTTGAAAAATACGGCAAGCTGCCGCCAAAGACCTGGGACGAGATG
GCCGCGACCGCCAAGGACGTTCAGGATAAGGAACGCCAGGCCGGACAGAAGGACCTTTGGGGTTATGTCTTCCAGGGCAA
CTCCTATGAAGGGCTGACCTGCAACGCGCTGGAATGGGTGAAGTCGTCGGGCGGCGGCCAGATTGTCGAGCCAGACGGGA
CAATTTCGATCAACAACGAGAAGGCGGCGGCAGCGCTTGAACGCGCCAAGGGCTGGATCGGGACGATCTCACCCCCAGGC
GTGCTCGCATATCAGGAAGAGGAATCGCGCGGCGTTTGGCAGACAGGCAACGCCGTCTTTATGCGCAACTGGCCCTATGC
CTATTCCCTCGGTAATGGTTCCGACAGCGCTGTGAAGGGCAAGTTCGACGTCATGACGTTGCCGGTTGCCGCGGCCGGCG
ACAAGCCGTCTTCGACGCTTGGCGGCTGGAATCTGGCGGTTTCGAAATATTCTGAAAAGCAGGAAGCAGCCATTGCGCTG
GTAAAATTCCTGGCGTCGAAGGACGTCCAAAAGGCGCGCGCCATCGAGCTGTCCAACCTGCCCACACTGACAGACCTCTA
TGATGACAAGGATGTTGCCGCCGCGCAGCCCTTCATGCCGAACTGGAAACCTATCTTCCAGGATGCCGTGCCGCGTCCTT
CGGCGACCGCCAAGGTAAAGTACAACGAAGTGTCTTCGAAGTTCTGGACGGCGGTGCACAACACGCTGTCAGGCAGCGGC
TCGGCTGCCGAGAACCTCGAGCTTCTCGAAGCCGACCTCACGACCCTCAAGGGCGACGCCTGGTAA

Upstream 100 bases:

>100_bases
GCGATCACAACTAAAAAGTGCCAATGTGGCGACTTGACGAGGTTGAAACCTTCTGCAATTGTCCGCGTGCCAAATCGATT
TCAATTTCTGGGAGGAGATT

Downstream 100 bases:

>100_bases
GATTCCGGCCGGCGGCTGTGAAGCCGCCGGCCCGGTTTCGAACGAGCGAACGGGAGAGGCGTTTCATGACTGAAATCGTA
GTTTCGCAAGCAGTTGAACC

Product: trehalosemaltose ABC transporter sugar-binding protein

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MKKFALSLSVLGVAIAFAGPIRAAEISFAANTTGKNVEFLNKQLAIFEKETGNQVKLVTMPSSSSEQFSQYRLWLAAGNK
DIDVYQTDVIWAPQLADQFIDLKDATKDVAGQFFPSIIASQTVNGRLVALPLFTDAPALFYRKDLLEKYGKLPPKTWDEM
AATAKDVQDKERQAGQKDLWGYVFQGNSYEGLTCNALEWVKSSGGGQIVEPDGTISINNEKAAAALERAKGWIGTISPPG
VLAYQEEESRGVWQTGNAVFMRNWPYAYSLGNGSDSAVKGKFDVMTLPVAAAGDKPSSTLGGWNLAVSKYSEKQEAAIAL
VKFLASKDVQKARAIELSNLPTLTDLYDDKDVAAAQPFMPNWKPIFQDAVPRPSATAKVKYNEVSSKFWTAVHNTLSGSG
SAAENLELLEADLTTLKGDAW

Sequences:

>Translated_421_residues
MKKFALSLSVLGVAIAFAGPIRAAEISFAANTTGKNVEFLNKQLAIFEKETGNQVKLVTMPSSSSEQFSQYRLWLAAGNK
DIDVYQTDVIWAPQLADQFIDLKDATKDVAGQFFPSIIASQTVNGRLVALPLFTDAPALFYRKDLLEKYGKLPPKTWDEM
AATAKDVQDKERQAGQKDLWGYVFQGNSYEGLTCNALEWVKSSGGGQIVEPDGTISINNEKAAAALERAKGWIGTISPPG
VLAYQEEESRGVWQTGNAVFMRNWPYAYSLGNGSDSAVKGKFDVMTLPVAAAGDKPSSTLGGWNLAVSKYSEKQEAAIAL
VKFLASKDVQKARAIELSNLPTLTDLYDDKDVAAAQPFMPNWKPIFQDAVPRPSATAKVKYNEVSSKFWTAVHNTLSGSG
SAAENLELLEADLTTLKGDAW
>Mature_421_residues
MKKFALSLSVLGVAIAFAGPIRAAEISFAANTTGKNVEFLNKQLAIFEKETGNQVKLVTMPSSSSEQFSQYRLWLAAGNK
DIDVYQTDVIWAPQLADQFIDLKDATKDVAGQFFPSIIASQTVNGRLVALPLFTDAPALFYRKDLLEKYGKLPPKTWDEM
AATAKDVQDKERQAGQKDLWGYVFQGNSYEGLTCNALEWVKSSGGGQIVEPDGTISINNEKAAAALERAKGWIGTISPPG
VLAYQEEESRGVWQTGNAVFMRNWPYAYSLGNGSDSAVKGKFDVMTLPVAAAGDKPSSTLGGWNLAVSKYSEKQEAAIAL
VKFLASKDVQKARAIELSNLPTLTDLYDDKDVAAAQPFMPNWKPIFQDAVPRPSATAKVKYNEVSSKFWTAVHNTLSGSG
SAAENLELLEADLTTLKGDAW

Specific function: Probably part of a binding-protein-dependent transport system [H]

COG id: COG1653

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

None

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 720 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 45773; Mature: 45773

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFALSLSVLGVAIAFAGPIRAAEISFAANTTGKNVEFLNKQLAIFEKETGNQVKLVTM
CCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHEEEEECCCCEEEEEEC
PSSSSEQFSQYRLWLAAGNKDIDVYQTDVIWAPQLADQFIDLKDATKDVAGQFFPSIIAS
CCCCHHHHHHEEEEEEECCCCCEEEEECEEECHHHHHHHHCHHHHHHHHHHHHHHHHHHH
QTVNGRLVALPLFTDAPALFYRKDLLEKYGKLPPKTWDEMAATAKDVQDKERQAGQKDLW
CCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
GYVFQGNSYEGLTCNALEWVKSSGGGQIVEPDGTISINNEKAAAALERAKGWIGTISPPG
EEEEECCCCCCEECHHHHHHHCCCCCEEECCCCEEEECCCHHHHHHHHHCCCEECCCCCC
VLAYQEEESRGVWQTGNAVFMRNWPYAYSLGNGSDSAVKGKFDVMTLPVAAAGDKPSSTL
EEEEECCCCCCCEECCCEEEEECCCEEEECCCCCCCCCCCEEEEEEEEEEECCCCCCCCC
GGWNLAVSKYSEKQEAAIALVKFLASKDVQKARAIELSNLPTLTDLYDDKDVAAAQPFMP
CCEEEEEHHHCHHHHHHHHHHHHHHHCCHHHHHHEECCCCCCHHHHCCCCCCCCCCCCCC
NWKPIFQDAVPRPSATAKVKYNEVSSKFWTAVHNTLSGSGSAAENLELLEADLTTLKGDA
CCCHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCC
W
C
>Mature Secondary Structure
MKKFALSLSVLGVAIAFAGPIRAAEISFAANTTGKNVEFLNKQLAIFEKETGNQVKLVTM
CCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHEEEEECCCCEEEEEEC
PSSSSEQFSQYRLWLAAGNKDIDVYQTDVIWAPQLADQFIDLKDATKDVAGQFFPSIIAS
CCCCHHHHHHEEEEEEECCCCCEEEEECEEECHHHHHHHHCHHHHHHHHHHHHHHHHHHH
QTVNGRLVALPLFTDAPALFYRKDLLEKYGKLPPKTWDEMAATAKDVQDKERQAGQKDLW
CCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
GYVFQGNSYEGLTCNALEWVKSSGGGQIVEPDGTISINNEKAAAALERAKGWIGTISPPG
EEEEECCCCCCEECHHHHHHHCCCCCEEECCCCEEEECCCHHHHHHHHHCCCEECCCCCC
VLAYQEEESRGVWQTGNAVFMRNWPYAYSLGNGSDSAVKGKFDVMTLPVAAAGDKPSSTL
EEEEECCCCCCCEECCCEEEEECCCEEEECCCCCCCCCCCEEEEEEEEEEECCCCCCCCC
GGWNLAVSKYSEKQEAAIALVKFLASKDVQKARAIELSNLPTLTDLYDDKDVAAAQPFMP
CCEEEEEHHHCHHHHHHHHHHHHHHHCCHHHHHHEECCCCCCHHHHCCCCCCCCCCCCCC
NWKPIFQDAVPRPSATAKVKYNEVSSKFWTAVHNTLSGSGSAAENLELLEADLTTLKGDA
CCCHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCC
W
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]