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The map label for this gene is hsdM [H]

Identifier: 189424479

GI number: 189424479

Start: 1460313

End: 1461746

Strand: Reverse

Name: hsdM [H]

Synonym: Glov_1415

Alternate gene names: 189424479

Gene position: 1461746-1460313 (Counterclockwise)

Preceding gene: 189424480

Following gene: 189424478

Centisome position: 37.31

GC content: 54.88

Gene sequence:

>1434_bases
ATGAAAAATATCGGCACCATCATCAAACGGCTGCAGAACACCATGCGCAAGGATCAAGGCGTCTCCGGTGATGCCCAACG
CATCGAACAGCTTGGCTGGATGATTACCCTGAAGATACTGGACGACAAGGACAAGGAGCTGGAGATACTTCAAGAGGCCT
ACGCCTCACCCATCCCTTCGGCGGTGCAGTGGCGGGCCTGGGCTGCCGATGCCGAGGGGATGACTGGTGACGAGCTTAAA
GAGTTCATCGACCTGAAGCTGTTTCCCGCACTGAAAAACCTGGACATCTCCACTGGCAACAAACGCGCCCTGATCATCCG
CGAGATATTCGAAGGCACCAACAACTACATGAAGAACGGCACCGTCATCCGCCAGGTGCTGAATGAGCTGAACCAGATCG
ATTTCAACTCCTCCGATGACCGCCATATTTTCGGCGACATTTACGAAACAATCCTGCGTGACCTGCAAAGCGCCGGCAAC
TATGGCGAGTTCTACACCCCCCGTGCCCTGACCGAGTTCATGACCGCCATCATCAACCCACGCCTGGGCGAAAAGGTGCT
CGACCCAGCCTGCGGCACCGGCGGCTTCCTCACCTGCGCAATTGAAAACATCCGTCGCCAGGATGTTAAGAACGTAGAAG
ATCTGCAAACCTTGCAGTCAACTATCCACGGTATGGAGTTTAAGCCGCTTCCATTCATGCTCAGCGTCACCAATATGATC
CTGCATGATATCGAAGTGCCGAATGTGGATTACACCGACAGCCTCAACCGGGAATACACCAGCATCGGCGCCAAGGATCG
GGTGGATGTGATCCTGGCCAATCCACCTTTCGGAGCATCAGTTACCGACGGCGTTGAAACAAATTTCCCCCTGAACTACC
GCACCACTGAAAGCGCCGATCTGTTTCTGCTCCTGATGATCCGCTATCTCAAGGATGGTGGTCGGGCCGCTATTGTCCTT
CCGGACGGCTCTCTGACCGGCGACGGCGTCAAGCAGCGTATCCGCCAGCACTGGCTGGAAGGCTGTAACCTGCATACGAT
TGTGCGGCTGCCCAACTCGGTCTTTCAGCCCTACGCCAGTGTTGCCACCAACTTGCTCTTTTTTACCAAGGGGGAGCCGA
CCAAGGAAATCTGGTACTGGGAGCATCAATTACCTGAAGGGGTCAAGTCCTATTCCAAGACCAAGCCGATCCAGAGCGCT
GAGTTCAACCGCCTAAAGGAATGGTGGAACAACCGGCAGGAGAGCGATCAAGCCTGGCGCGTTTCTATCGATACCCTGAC
TGCCAACAGCTATAACCTGGATACAAAGAACCCGCACATTAAGGACACCGGCCACGCCCACACCAGCGCGGAACTGTTGG
AGCTGCTGCACCAGTCGTTTCAGAAGAGTGATAGCCTTTTGCAGACGTTGCGGAAGGAATTGGCCTGTGTCTAA

Upstream 100 bases:

>100_bases
CGGCACCCCGGCTGAGATCGTCCATCTCTTCGGCGGCAAGGAAGCCTATCAGGCGGCAGTGAGAGAGCTGGAAACTGAAA
TTTACAGGATGGCGTAATTC

Downstream 100 bases:

>100_bases
CTGGAAACGAGCCAGAATAGGTGATCTGTGTGAGATCATCAAAGGGGAAACCGGCCTGGCAAGCGCTCCTCCAGGGGAAT
ACCCACTGGTTGCCACTGGC

Product: N-6 DNA methylase

Products: NA

Alternate protein names: M.EcoEI [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MKNIGTIIKRLQNTMRKDQGVSGDAQRIEQLGWMITLKILDDKDKELEILQEAYASPIPSAVQWRAWAADAEGMTGDELK
EFIDLKLFPALKNLDISTGNKRALIIREIFEGTNNYMKNGTVIRQVLNELNQIDFNSSDDRHIFGDIYETILRDLQSAGN
YGEFYTPRALTEFMTAIINPRLGEKVLDPACGTGGFLTCAIENIRRQDVKNVEDLQTLQSTIHGMEFKPLPFMLSVTNMI
LHDIEVPNVDYTDSLNREYTSIGAKDRVDVILANPPFGASVTDGVETNFPLNYRTTESADLFLLLMIRYLKDGGRAAIVL
PDGSLTGDGVKQRIRQHWLEGCNLHTIVRLPNSVFQPYASVATNLLFFTKGEPTKEIWYWEHQLPEGVKSYSKTKPIQSA
EFNRLKEWWNNRQESDQAWRVSIDTLTANSYNLDTKNPHIKDTGHAHTSAELLELLHQSFQKSDSLLQTLRKELACV

Sequences:

>Translated_477_residues
MKNIGTIIKRLQNTMRKDQGVSGDAQRIEQLGWMITLKILDDKDKELEILQEAYASPIPSAVQWRAWAADAEGMTGDELK
EFIDLKLFPALKNLDISTGNKRALIIREIFEGTNNYMKNGTVIRQVLNELNQIDFNSSDDRHIFGDIYETILRDLQSAGN
YGEFYTPRALTEFMTAIINPRLGEKVLDPACGTGGFLTCAIENIRRQDVKNVEDLQTLQSTIHGMEFKPLPFMLSVTNMI
LHDIEVPNVDYTDSLNREYTSIGAKDRVDVILANPPFGASVTDGVETNFPLNYRTTESADLFLLLMIRYLKDGGRAAIVL
PDGSLTGDGVKQRIRQHWLEGCNLHTIVRLPNSVFQPYASVATNLLFFTKGEPTKEIWYWEHQLPEGVKSYSKTKPIQSA
EFNRLKEWWNNRQESDQAWRVSIDTLTANSYNLDTKNPHIKDTGHAHTSAELLELLHQSFQKSDSLLQTLRKELACV
>Mature_477_residues
MKNIGTIIKRLQNTMRKDQGVSGDAQRIEQLGWMITLKILDDKDKELEILQEAYASPIPSAVQWRAWAADAEGMTGDELK
EFIDLKLFPALKNLDISTGNKRALIIREIFEGTNNYMKNGTVIRQVLNELNQIDFNSSDDRHIFGDIYETILRDLQSAGN
YGEFYTPRALTEFMTAIINPRLGEKVLDPACGTGGFLTCAIENIRRQDVKNVEDLQTLQSTIHGMEFKPLPFMLSVTNMI
LHDIEVPNVDYTDSLNREYTSIGAKDRVDVILANPPFGASVTDGVETNFPLNYRTTESADLFLLLMIRYLKDGGRAAIVL
PDGSLTGDGVKQRIRQHWLEGCNLHTIVRLPNSVFQPYASVATNLLFFTKGEPTKEIWYWEHQLPEGVKSYSKTKPIQSA
EFNRLKEWWNNRQESDQAWRVSIDTLTANSYNLDTKNPHIKDTGHAHTSAELLELLHQSFQKSDSLLQTLRKELACV

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=406, Percent_Identity=28.0788177339901, Blast_Score=139, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 54157; Mature: 54157

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIGTIIKRLQNTMRKDQGVSGDAQRIEQLGWMITLKILDDKDKELEILQEAYASPIPS
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHCCCCCC
AVQWRAWAADAEGMTGDELKEFIDLKLFPALKNLDISTGNKRALIIREIFEGTNNYMKNG
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHCHHHHHHCC
TVIRQVLNELNQIDFNSSDDRHIFGDIYETILRDLQSAGNYGEFYTPRALTEFMTAIINP
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCC
RLGEKVLDPACGTGGFLTCAIENIRRQDVKNVEDLQTLQSTIHGMEFKPLPFMLSVTNMI
CCCHHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
LHDIEVPNVDYTDSLNREYTSIGAKDRVDVILANPPFGASVTDGVETNFPLNYRTTESAD
HHHCCCCCCCCCCCCCCHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCH
LFLLLMIRYLKDGGRAAIVLPDGSLTGDGVKQRIRQHWLEGCNLHTIVRLPNSVFQPYAS
HHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHH
VATNLLFFTKGEPTKEIWYWEHQLPEGVKSYSKTKPIQSAEFNRLKEWWNNRQESDQAWR
HHHCEEEEECCCCCHHHEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCEEE
VSIDTLTANSYNLDTKNPHIKDTGHAHTSAELLELLHQSFQKSDSLLQTLRKELACV
EEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKNIGTIIKRLQNTMRKDQGVSGDAQRIEQLGWMITLKILDDKDKELEILQEAYASPIPS
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHCCCCCC
AVQWRAWAADAEGMTGDELKEFIDLKLFPALKNLDISTGNKRALIIREIFEGTNNYMKNG
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHCHHHHHHCC
TVIRQVLNELNQIDFNSSDDRHIFGDIYETILRDLQSAGNYGEFYTPRALTEFMTAIINP
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCC
RLGEKVLDPACGTGGFLTCAIENIRRQDVKNVEDLQTLQSTIHGMEFKPLPFMLSVTNMI
CCCHHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
LHDIEVPNVDYTDSLNREYTSIGAKDRVDVILANPPFGASVTDGVETNFPLNYRTTESAD
HHHCCCCCCCCCCCCCCHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCH
LFLLLMIRYLKDGGRAAIVLPDGSLTGDGVKQRIRQHWLEGCNLHTIVRLPNSVFQPYAS
HHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHH
VATNLLFFTKGEPTKEIWYWEHQLPEGVKSYSKTKPIQSAEFNRLKEWWNNRQESDQAWR
HHHCEEEEECCCCCHHHEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCEEE
VSIDTLTANSYNLDTKNPHIKDTGHAHTSAELLELLHQSFQKSDSLLQTLRKELACV
EEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8412658 [H]