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The map label for this gene is hsdS [H]

Identifier: 189424478

GI number: 189424478

Start: 1458977

End: 1460320

Strand: Reverse

Name: hsdS [H]

Synonym: Glov_1414

Alternate gene names: 189424478

Gene position: 1460320-1458977 (Counterclockwise)

Preceding gene: 189424479

Following gene: 189424477

Centisome position: 37.27

GC content: 51.49

Gene sequence:

>1344_bases
GTGTCTAACTGGAAACGAGCCAGAATAGGTGATCTGTGTGAGATCATCAAAGGGGAAACCGGCCTGGCAAGCGCTCCTCC
AGGGGAATACCCACTGGTTGCCACTGGCGCAGATCGCAGATCATGCACCACCTGGCAGTTTGATACCGATGCGGTTTGCA
TTCCACTGGTGTCATCAACCGGTCATGGTAAAAAGACGCTGAATTATGTCCACTATCAGTCAGGCAAGTTTGCTCTCGGC
ACCATTCTGGCTGCCGTCATTCCCAAAGACCCGTCTGTTCTGACAGCTCGCTTTCTGCATCTGTATCTTTCCCATTTCAA
GGATACCGTTCTGGTGCCGTTGATGAAGGGAGCAGCCAATGTCAGTCTGTCGATGAAAGAGATTGCATCGGTTAAGATCC
CCGTGCCACCGCTGGATGAACAGCAGAGTCTGATAGACCTGATATTCCGGATTGAGGATGAGCATCAGGAATTGCTGACG
GAAACGAACCATCAAGGAGTGCTGCTCAAACAACTCCGCCAGGCTCTGCTGCAAGAGGCTGTCGCAGGGGAACTGACAAC
GGCATGGCGCAAGCAACATCCGGTGGCAAAGGGCGATCCGCAGTATGACGCGGCAGCGTTGCTGGCACAGATCAAGGCAG
AAAAAGAGCGGCTGGTGAAGGAAGGTAAAATCCGGAAAGAAAAGCCCTTGCCACCAATTACCGACGAAGATAAACCGTTC
GACCTACCGGAGGGTTGGGGGTGGTGCCGGCTGGGAGAAGTGGCAGACGGCTTCCAGTACGGTTCGAGTGTGAAGTCTCT
CAAGGAAGGGAAAGTTCCAGTTCTTCGCATGGGCAACATTCAATGTGGGAAAATAGACTGGAGCAACCTTGTCTACACTA
ACGATACAGGTGAAATTAGGAAATATCGCGTTACAAATGGCGACCTTCTTTTCAATCGTACGAACAGCAGAGAGCTTGTT
GGTAAGACAGGACTCTTTGACGGTATGTACGAGGCCATTTTCGCTGGCTATTTGGTGCGGGTAACGATGTTAGGCGGCAT
CAGTGCAACCTACTCAAACGGGGTCCTCAACTCAAAGTTTCATCGTGAGTGGTGCGATGCGAACAAGACAGATGCATTGG
GTCAATCAAATATTAATGCCACAAAACTGAGGGATTATTTTTTCCCTCTTCCCCCTCTCGCCGAACAACAAGCCATCGTT
GCCCGCGTGGATAGTCTCATGGCAACCATTGATGAACTGGAAAAGCAGGTTGCCGAACGGAAGGAGCAGGCGCAGTTGCT
GATGCAGACGGTGCTGCGGGAGGCGTTTGATGTGGGGCAACAAGCGGGAACTGTTATTTCTTAA

Upstream 100 bases:

>100_bases
ACCGGCCACGCCCACACCAGCGCGGAACTGTTGGAGCTGCTGCACCAGTCGTTTCAGAAGAGTGATAGCCTTTTGCAGAC
GTTGCGGAAGGAATTGGCCT

Downstream 100 bases:

>100_bases
TTGTCACAACTGAACTGAGGAGCGCAACTATGCCTAAGTATACCGGATCTGCCGATGTTTACCGAGAGCTTGTTGAGGAT
TCCAAAGAAAACTGGTTATA

Product: restriction modification system DNA specificity domain-containing protein

Products: NA

Alternate protein names: S.EcoAI; Type I restriction enzyme EcoAI specificity protein; S protein [H]

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MSNWKRARIGDLCEIIKGETGLASAPPGEYPLVATGADRRSCTTWQFDTDAVCIPLVSSTGHGKKTLNYVHYQSGKFALG
TILAAVIPKDPSVLTARFLHLYLSHFKDTVLVPLMKGAANVSLSMKEIASVKIPVPPLDEQQSLIDLIFRIEDEHQELLT
ETNHQGVLLKQLRQALLQEAVAGELTTAWRKQHPVAKGDPQYDAAALLAQIKAEKERLVKEGKIRKEKPLPPITDEDKPF
DLPEGWGWCRLGEVADGFQYGSSVKSLKEGKVPVLRMGNIQCGKIDWSNLVYTNDTGEIRKYRVTNGDLLFNRTNSRELV
GKTGLFDGMYEAIFAGYLVRVTMLGGISATYSNGVLNSKFHREWCDANKTDALGQSNINATKLRDYFFPLPPLAEQQAIV
ARVDSLMATIDELEKQVAERKEQAQLLMQTVLREAFDVGQQAGTVIS

Sequences:

>Translated_447_residues
MSNWKRARIGDLCEIIKGETGLASAPPGEYPLVATGADRRSCTTWQFDTDAVCIPLVSSTGHGKKTLNYVHYQSGKFALG
TILAAVIPKDPSVLTARFLHLYLSHFKDTVLVPLMKGAANVSLSMKEIASVKIPVPPLDEQQSLIDLIFRIEDEHQELLT
ETNHQGVLLKQLRQALLQEAVAGELTTAWRKQHPVAKGDPQYDAAALLAQIKAEKERLVKEGKIRKEKPLPPITDEDKPF
DLPEGWGWCRLGEVADGFQYGSSVKSLKEGKVPVLRMGNIQCGKIDWSNLVYTNDTGEIRKYRVTNGDLLFNRTNSRELV
GKTGLFDGMYEAIFAGYLVRVTMLGGISATYSNGVLNSKFHREWCDANKTDALGQSNINATKLRDYFFPLPPLAEQQAIV
ARVDSLMATIDELEKQVAERKEQAQLLMQTVLREAFDVGQQAGTVIS
>Mature_446_residues
SNWKRARIGDLCEIIKGETGLASAPPGEYPLVATGADRRSCTTWQFDTDAVCIPLVSSTGHGKKTLNYVHYQSGKFALGT
ILAAVIPKDPSVLTARFLHLYLSHFKDTVLVPLMKGAANVSLSMKEIASVKIPVPPLDEQQSLIDLIFRIEDEHQELLTE
TNHQGVLLKQLRQALLQEAVAGELTTAWRKQHPVAKGDPQYDAAALLAQIKAEKERLVKEGKIRKEKPLPPITDEDKPFD
LPEGWGWCRLGEVADGFQYGSSVKSLKEGKVPVLRMGNIQCGKIDWSNLVYTNDTGEIRKYRVTNGDLLFNRTNSRELVG
KTGLFDGMYEAIFAGYLVRVTMLGGISATYSNGVLNSKFHREWCDANKTDALGQSNINATKLRDYFFPLPPLAEQQAIVA
RVDSLMATIDELEKQVAERKEQAQLLMQTVLREAFDVGQQAGTVIS

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

Organism=Escherichia coli, GI1790807, Length=168, Percent_Identity=28.5714285714286, Blast_Score=73, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 49542; Mature: 49411

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNWKRARIGDLCEIIKGETGLASAPPGEYPLVATGADRRSCTTWQFDTDAVCIPLVSST
CCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCEEEEECCCCEEEEEECCC
GHGKKTLNYVHYQSGKFALGTILAAVIPKDPSVLTARFLHLYLSHFKDTVLVPLMKGAAN
CCCCHHEEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VSLSMKEIASVKIPVPPLDEQQSLIDLIFRIEDEHQELLTETNHQGVLLKQLRQALLQEA
CEEEHHHHCEEECCCCCCCCHHHHHHHHHHHCHHHHHHHHHCCCCCHHHHHHHHHHHHHH
VAGELTTAWRKQHPVAKGDPQYDAAALLAQIKAEKERLVKEGKIRKEKPLPPITDEDKPF
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
DLPEGWGWCRLGEVADGFQYGSSVKSLKEGKVPVLRMGNIQCGKIDWSNLVYTNDTGEIR
CCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEECCEECCCEEEECCCCCEE
KYRVTNGDLLFNRTNSRELVGKTGLFDGMYEAIFAGYLVRVTMLGGISATYSNGVLNSKF
EEEECCCCEEEECCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHH
HREWCDANKTDALGQSNINATKLRDYFFPLPPLAEQQAIVARVDSLMATIDELEKQVAER
HHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KEQAQLLMQTVLREAFDVGQQAGTVIS
HHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SNWKRARIGDLCEIIKGETGLASAPPGEYPLVATGADRRSCTTWQFDTDAVCIPLVSST
CCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCEEEEECCCCEEEEEECCC
GHGKKTLNYVHYQSGKFALGTILAAVIPKDPSVLTARFLHLYLSHFKDTVLVPLMKGAAN
CCCCHHEEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VSLSMKEIASVKIPVPPLDEQQSLIDLIFRIEDEHQELLTETNHQGVLLKQLRQALLQEA
CEEEHHHHCEEECCCCCCCCHHHHHHHHHHHCHHHHHHHHHCCCCCHHHHHHHHHHHHHH
VAGELTTAWRKQHPVAKGDPQYDAAALLAQIKAEKERLVKEGKIRKEKPLPPITDEDKPF
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
DLPEGWGWCRLGEVADGFQYGSSVKSLKEGKVPVLRMGNIQCGKIDWSNLVYTNDTGEIR
CCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEECCEECCCEEEECCCCCEE
KYRVTNGDLLFNRTNSRELVGKTGLFDGMYEAIFAGYLVRVTMLGGISATYSNGVLNSKF
EEEECCCCEEEECCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHH
HREWCDANKTDALGQSNINATKLRDYFFPLPPLAEQQAIVARVDSLMATIDELEKQVAER
HHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KEQAQLLMQTVLREAFDVGQQAGTVIS
HHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2642743 [H]