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The map label for this gene is rpoD [H]

Identifier: 189424079

GI number: 189424079

Start: 1052745

End: 1054505

Strand: Reverse

Name: rpoD [H]

Synonym: Glov_1013

Alternate gene names: 189424079

Gene position: 1054505-1052745 (Counterclockwise)

Preceding gene: 189424080

Following gene: 189424078

Centisome position: 26.92

GC content: 55.65

Gene sequence:

>1761_bases
ATGGCTAAGAAAAATATTGATGAAGTCAAGCAGTTGATTGATATCGGCAAGGAAAAGGGCTTTTTGACCTTTGACGAGGT
AAACGACATTCTCCCGCCTGATATCGCCACCGAGCAGATCGATGACGTGATGGGGATGTTCGGAGACATGGATATCGAGA
TCGTTGACTCGGCCCAGAAGGTCAAGATTCCCAAGATCAAGATCGATCTGGAAGAAGGGGAAGAAGAGGCCGAAGGTGAG
GCTGAAGAGGTAGAGTTTGAGCCGGGCGCCATCGGACGCACCAGTGACCCGGTCCGGATGTACCTGCGTGAAATGGGCTC
GGTCTCCCTGCTGACCCGTGAGGGTGAGGTTGAGATTGCCAAGCGGATTGAAGACGGTGACCGGGAAGTGGCCGGCATCA
TCCTCAACACCCCGATTACGGTTAAGGAAGTACTGGCGCTGGGTGAAAAACTGCGCAAGTTCCAGATCACCCCTTCAGAG
ATCAGCAAAGACATTGAAGAGGATGCTGATGTTGATATCGAAGAGGGGGAAGAGGATATCCAGCAGATCCGCCTGCTGGA
GACGATTGACGCCATTGCCGCCAGTGAGCAGGAGATCAACGAACTGTTGACCGCGCTGGAGTCGGCCAAGGCAGCTGCCA
AGAAAAAGGAACTGGAAAAGAGCCTGAAGGAGGCCAAGGAGCATCTGGCAGAACTGCTGCGTTCGCTGCGGATGAAGGAC
CGCCATATCACCAGGGTTGCCGACCGCCTGAAAGAACTCTCCTCCAAGGTTAACCGGGTCAAGAATGAGCTGGCCGAGGT
GCATAATGTTGTGCCGCCTGACAAGCTTGCAGAGCTGCTGGAAAAGTTCAGAGCCGATGAGGCAAAAGGGATGGCCGCCC
TCAAACCGCTCAAACTGAGTGATGAGGACTTCCAGAAAGTCGAGAAGCGCCTCAAGAATGCCGCCCGTAAGCTGACCAAG
GTTGAACAGGAATCCGGTTTCAAGGCAACCGAGCTTTCCAAGGCGATCCGGGCGATCCGCGAAGGTGAGCATAAGGCACG
CATCGCCAAATCAGAGCTGATTGAAGCCAACCTGCGTCTGGTGGTCTCGATTGCCAAAAAATACACCAACCGCGGCCTGC
AGTTCCTCGACCTGATTCAGGAAGGCAACATCGGCCTGATGAAGGCGGTGGACAAGTTTGAATACCAGCGTGGCTACAAA
TTCTCAACCTACGCCACCTGGTGGATCCGTCAGGCCATCACCCGCGCCATCGCCGACCAGGCCCGAACCATCCGGATCCC
GGTCCATATGATCGAGACCATCAACAAGTTGATCCGCACCAGTCGCCAGCTGGTGCAGGAAAATGGCCGCGAACCGTCGC
CGGAAGAGATTGCCGACCGGATGCAGCTGCCGCTGGACAAGGTGCGCAAGGTGCTGAAGATCGCCAAGGAGCCGATCTCG
CTGGAAACCCCGATCGGTGAAGAAGAGGATTCGCACCTGGGTGATTTCATTGAAGACAAGGGAGTGGTCAACCCCCTTGA
GGCGGTGATCAAGGCCAACCTGTCCGAGCAGACCTCCCGCGTGCTTTCAACCCTGACCCCCCGGGAGGAAAAGGTGTTGC
GGATGCGTTTCGGCATTGGCGAAAAAAGCGACCACACCCTGGAAGAGGTGGGCCAGGACTTTGAGGTTACCCGTGAGCGG
ATTCGTCAGATCGAGGCCAAGGCACTGCGCAAACTGCGCCACCCCAGCCGCAGTAAAAAACTGAAAAGCTTTGTAGAGTA
A

Upstream 100 bases:

>100_bases
GAAGAGTATCAAGAACTGTTAAAAACAGCCGATCAGTTGCGTAATCAAAAATCGGCGTTATAAGTAACCAGACTCGTATT
TCATAAAGGTACCCACCCAC

Downstream 100 bases:

>100_bases
CCAGATGAGACACTCCTTCAGCCACTGTCAGCCGCTCCGTTTTCTCGTTTGCCTGTACCTGCTGCTCTGTCTGCTGGCAC
CAGCACCGCTCAGGGCCGAG

Product: RpoD subfamily RNA polymerase sigma-70 subunit

Products: NA

Alternate protein names: Sigma-80 [H]

Number of amino acids: Translated: 586; Mature: 585

Protein sequence:

>586_residues
MAKKNIDEVKQLIDIGKEKGFLTFDEVNDILPPDIATEQIDDVMGMFGDMDIEIVDSAQKVKIPKIKIDLEEGEEEAEGE
AEEVEFEPGAIGRTSDPVRMYLREMGSVSLLTREGEVEIAKRIEDGDREVAGIILNTPITVKEVLALGEKLRKFQITPSE
ISKDIEEDADVDIEEGEEDIQQIRLLETIDAIAASEQEINELLTALESAKAAAKKKELEKSLKEAKEHLAELLRSLRMKD
RHITRVADRLKELSSKVNRVKNELAEVHNVVPPDKLAELLEKFRADEAKGMAALKPLKLSDEDFQKVEKRLKNAARKLTK
VEQESGFKATELSKAIRAIREGEHKARIAKSELIEANLRLVVSIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYQRGYK
FSTYATWWIRQAITRAIADQARTIRIPVHMIETINKLIRTSRQLVQENGREPSPEEIADRMQLPLDKVRKVLKIAKEPIS
LETPIGEEEDSHLGDFIEDKGVVNPLEAVIKANLSEQTSRVLSTLTPREEKVLRMRFGIGEKSDHTLEEVGQDFEVTRER
IRQIEAKALRKLRHPSRSKKLKSFVE

Sequences:

>Translated_586_residues
MAKKNIDEVKQLIDIGKEKGFLTFDEVNDILPPDIATEQIDDVMGMFGDMDIEIVDSAQKVKIPKIKIDLEEGEEEAEGE
AEEVEFEPGAIGRTSDPVRMYLREMGSVSLLTREGEVEIAKRIEDGDREVAGIILNTPITVKEVLALGEKLRKFQITPSE
ISKDIEEDADVDIEEGEEDIQQIRLLETIDAIAASEQEINELLTALESAKAAAKKKELEKSLKEAKEHLAELLRSLRMKD
RHITRVADRLKELSSKVNRVKNELAEVHNVVPPDKLAELLEKFRADEAKGMAALKPLKLSDEDFQKVEKRLKNAARKLTK
VEQESGFKATELSKAIRAIREGEHKARIAKSELIEANLRLVVSIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYQRGYK
FSTYATWWIRQAITRAIADQARTIRIPVHMIETINKLIRTSRQLVQENGREPSPEEIADRMQLPLDKVRKVLKIAKEPIS
LETPIGEEEDSHLGDFIEDKGVVNPLEAVIKANLSEQTSRVLSTLTPREEKVLRMRFGIGEKSDHTLEEVGQDFEVTRER
IRQIEAKALRKLRHPSRSKKLKSFVE
>Mature_585_residues
AKKNIDEVKQLIDIGKEKGFLTFDEVNDILPPDIATEQIDDVMGMFGDMDIEIVDSAQKVKIPKIKIDLEEGEEEAEGEA
EEVEFEPGAIGRTSDPVRMYLREMGSVSLLTREGEVEIAKRIEDGDREVAGIILNTPITVKEVLALGEKLRKFQITPSEI
SKDIEEDADVDIEEGEEDIQQIRLLETIDAIAASEQEINELLTALESAKAAAKKKELEKSLKEAKEHLAELLRSLRMKDR
HITRVADRLKELSSKVNRVKNELAEVHNVVPPDKLAELLEKFRADEAKGMAALKPLKLSDEDFQKVEKRLKNAARKLTKV
EQESGFKATELSKAIRAIREGEHKARIAKSELIEANLRLVVSIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYQRGYKF
STYATWWIRQAITRAIADQARTIRIPVHMIETINKLIRTSRQLVQENGREPSPEEIADRMQLPLDKVRKVLKIAKEPISL
ETPIGEEEDSHLGDFIEDKGVVNPLEAVIKANLSEQTSRVLSTLTPREEKVLRMRFGIGEKSDHTLEEVGQDFEVTRERI
RQIEAKALRKLRHPSRSKKLKSFVE

Specific function: Also required for extracellular A-signal production. The A-signal is the first in a series of extracellular signals that are required for myxspore differentiation in M.xanthus. It is thought to act as a cell-density signal during early development to ensu

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=611, Percent_Identity=46.9721767594108, Blast_Score=483, Evalue=1e-137,
Organism=Escherichia coli, GI1789098, Length=234, Percent_Identity=42.7350427350427, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1789871, Length=246, Percent_Identity=28.8617886178862, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788231, Length=202, Percent_Identity=29.2079207920792, Blast_Score=76, Evalue=7e-15,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 66524; Mature: 66393

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2 ; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKNIDEVKQLIDIGKEKGFLTFDEVNDILPPDIATEQIDDVMGMFGDMDIEIVDSAQK
CCCCCHHHHHHHHHCCCCCCCEEHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCHHH
VKIPKIKIDLEEGEEEAEGEAEEVEFEPGAIGRTSDPVRMYLREMGSVSLLTREGEVEIA
EECCEEEEECCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCHHHHH
KRIEDGDREVAGIILNTPITVKEVLALGEKLRKFQITPSEISKDIEEDADVDIEEGEEDI
HHHCCCCCCEEHEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHCCHHHH
QQIRLLETIDAIAASEQEINELLTALESAKAAAKKKELEKSLKEAKEHLAELLRSLRMKD
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHITRVADRLKELSSKVNRVKNELAEVHNVVPPDKLAELLEKFRADEAKGMAALKPLKLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHCCHHHHCCCCCC
DEDFQKVEKRLKNAARKLTKVEQESGFKATELSKAIRAIREGEHKARIAKSELIEANLRL
CHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
VVSIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYQRGYKFSTYATWWIRQAITRAIADQ
HHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHC
ARTIRIPVHMIETINKLIRTSRQLVQENGREPSPEEIADRMQLPLDKVRKVLKIAKEPIS
CCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCC
LETPIGEEEDSHLGDFIEDKGVVNPLEAVIKANLSEQTSRVLSTLTPREEKVLRMRFGIG
CCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHCCC
EKSDHTLEEVGQDFEVTRERIRQIEAKALRKLRHPSRSKKLKSFVE
CCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure 
AKKNIDEVKQLIDIGKEKGFLTFDEVNDILPPDIATEQIDDVMGMFGDMDIEIVDSAQK
CCCCHHHHHHHHHCCCCCCCEEHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCHHH
VKIPKIKIDLEEGEEEAEGEAEEVEFEPGAIGRTSDPVRMYLREMGSVSLLTREGEVEIA
EECCEEEEECCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCHHHHH
KRIEDGDREVAGIILNTPITVKEVLALGEKLRKFQITPSEISKDIEEDADVDIEEGEEDI
HHHCCCCCCEEHEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHCCHHHH
QQIRLLETIDAIAASEQEINELLTALESAKAAAKKKELEKSLKEAKEHLAELLRSLRMKD
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHITRVADRLKELSSKVNRVKNELAEVHNVVPPDKLAELLEKFRADEAKGMAALKPLKLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHCCHHHHCCCCCC
DEDFQKVEKRLKNAARKLTKVEQESGFKATELSKAIRAIREGEHKARIAKSELIEANLRL
CHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
VVSIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYQRGYKFSTYATWWIRQAITRAIADQ
HHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHC
ARTIRIPVHMIETINKLIRTSRQLVQENGREPSPEEIADRMQLPLDKVRKVLKIAKEPIS
CCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCC
LETPIGEEEDSHLGDFIEDKGVVNPLEAVIKANLSEQTSRVLSTLTPREEKVLRMRFGIG
CCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHCCC
EKSDHTLEEVGQDFEVTRERIRQIEAKALRKLRHPSRSKKLKSFVE
CCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2104614 [H]