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The map label for this gene is dnaG [H]

Identifier: 189424080

GI number: 189424080

Start: 1054543

End: 1056309

Strand: Reverse

Name: dnaG [H]

Synonym: Glov_1014

Alternate gene names: 189424080

Gene position: 1056309-1054543 (Counterclockwise)

Preceding gene: 189424081

Following gene: 189424079

Centisome position: 26.96

GC content: 59.71

Gene sequence:

>1767_bases
ATGAACCGCAATGACACCGTCAGAGAGGTTGCAGAGCGACTCTCCATAGCCGAGATTATCGGCGAATATGTTTCATTGAA
ACGTAGCGGCTCCAATTTTCTGGGGCTTTGTCCGTTTCATGGCGAGAAGACCCCGTCGTTCAACGTCAATCCGGCCAGAG
AGATCTTCCACTGTTTTGGCTGCGGTGCCGGCGGAGACATCTTCTCCTTTGTAATGAAGATTGAGGGAATCAGCTTCCCG
GAGGCCTTGCGCAAGCTGGCTGCGCGTGCCGGTGTGGCTATTGAAGATCGCCCTTTGACCGACGCCGAAAAGCAGCTCAA
GACGGAACGCGATCAGCAGCGCGCCATCATGCTCCTGACGGCACAACATTATCGCGAGACCCTGACCCGCCAACCGGAAG
GGGCAGTGGCGCGCAGTTATCTGCAAGAGCGGGAAGTGGACCCGGAAACCGCGGCAGCCTATGGACTGGGATACGCCCCG
GAACGGCGCGACACCCTGATCCAGCTCCTGCGGGCCAAGGGGCTGCCACTTGAACAGGCAGAACAGCTGGGGATCATCCG
TAGGGGAGACCGGGGCTGGTATGACCTGCTGCACCATCGCCTGATCTTTCCGATCCGCGACAAGCAGGGACAACCGATCG
GCTTTGCCGGCAGGGTACTGGACAACAGCCTGCCCAAGTACATCAACTCGCCGGAATCGCCGCTGTATCGCAAGAGCAGT
GTCCTGTTCGGAGTTGACCTGGCCCTGCGGGATATCAGGCAGAGCGGCACCGCCATTATCGTGGAAGGCTATTTCGACCA
TCTGGCCCTCTACCGGGCCGGGATACGCAATGCCCTGGCCACCTGCGGTACGGCGCTGACCGACGGCCATATCAGCCTGC
TGAAGAAACATGCCCAGCGGGTCTGCCTGCTGTTTGACGGTGACAATGCCGGGCGTAAGGCCACGGTACGGGCCATGGAA
CTCTGTCTTGAACAGGCCCTGCCGGTCTACGTGATCAACCTGCCCCAGGGCGAAGATCCCGACAGCCTGTTACAGAAGCA
TGGCGTCGAGGCGTTCAACCAGCGGGTTGCAGCGGCAAAACCGGCCTTTGAACAGTTTTTGCACTGGCTGCTGGCCAAGA
CACCGGCTGACAGCATTGATCAGCGGGTGCGCTTGATGGATGAGATCATCCCCCGCTTCCAGCGTATCAAGGATCCGGTG
GAGCGAGACCTGTACCAGAAAGAGATCTGCCGGCTGCTGGGGGTCGATCTCCAGGCCTTCCGGCGACGCCTGGCAGGTCA
GCGTGACCAGCAGCAACCGGTGGCACAACGACAGACGGCAACAAAACCGGTCACACAGACAATCGCACGGGACAGCACCC
AGGAAACCATGCTGGGCCTGCTGGCAGCCTATCCTGAAGCCCGTTTGGAGATCAGGACGCTTGATCTGCAGCAGCTGTTT
GACCAGCAGCACCTGCCCCTGGCCCAGGCGATCCTGGCGGCAGGTGAAGGAGAACATGACGGTCCGCTCTGGCCGGAGGT
GCTGGCACGGCTTGAGCAGGAACAACAGAAGGAGCTGGTGGCACGGCTGCTGATACAGGATCAGCACCTGGCCGGTATCG
ACTGGCGCGCTGCCCTGCGGCAGTGTCTGAAACGCCAGAAGCAGGGTGAGACCCGCGGCCTGAAACAGATTGCCCTCAGG
CTGGCCACCCTGCCGGAAGATAGTGAAGAGTATCAAGAACTGTTAAAAACAGCCGATCAGTTGCGTAATCAAAAATCGGC
GTTATAA

Upstream 100 bases:

>100_bases
CGTTCAAGGAAACCGTTCAAAAACATCCCTTTGCCCATCCGCTGGCAAGCTTTGGCGGCTCAACGCTTGATCGGGCCAAG
ACGCTACGGCAGCAGTCACC

Downstream 100 bases:

>100_bases
GTAACCAGACTCGTATTTCATAAAGGTACCCACCCACATGGCTAAGAAAAATATTGATGAAGTCAAGCAGTTGATTGATA
TCGGCAAGGAAAAGGGCTTT

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 588; Mature: 588

Protein sequence:

>588_residues
MNRNDTVREVAERLSIAEIIGEYVSLKRSGSNFLGLCPFHGEKTPSFNVNPAREIFHCFGCGAGGDIFSFVMKIEGISFP
EALRKLAARAGVAIEDRPLTDAEKQLKTERDQQRAIMLLTAQHYRETLTRQPEGAVARSYLQEREVDPETAAAYGLGYAP
ERRDTLIQLLRAKGLPLEQAEQLGIIRRGDRGWYDLLHHRLIFPIRDKQGQPIGFAGRVLDNSLPKYINSPESPLYRKSS
VLFGVDLALRDIRQSGTAIIVEGYFDHLALYRAGIRNALATCGTALTDGHISLLKKHAQRVCLLFDGDNAGRKATVRAME
LCLEQALPVYVINLPQGEDPDSLLQKHGVEAFNQRVAAAKPAFEQFLHWLLAKTPADSIDQRVRLMDEIIPRFQRIKDPV
ERDLYQKEICRLLGVDLQAFRRRLAGQRDQQQPVAQRQTATKPVTQTIARDSTQETMLGLLAAYPEARLEIRTLDLQQLF
DQQHLPLAQAILAAGEGEHDGPLWPEVLARLEQEQQKELVARLLIQDQHLAGIDWRAALRQCLKRQKQGETRGLKQIALR
LATLPEDSEEYQELLKTADQLRNQKSAL

Sequences:

>Translated_588_residues
MNRNDTVREVAERLSIAEIIGEYVSLKRSGSNFLGLCPFHGEKTPSFNVNPAREIFHCFGCGAGGDIFSFVMKIEGISFP
EALRKLAARAGVAIEDRPLTDAEKQLKTERDQQRAIMLLTAQHYRETLTRQPEGAVARSYLQEREVDPETAAAYGLGYAP
ERRDTLIQLLRAKGLPLEQAEQLGIIRRGDRGWYDLLHHRLIFPIRDKQGQPIGFAGRVLDNSLPKYINSPESPLYRKSS
VLFGVDLALRDIRQSGTAIIVEGYFDHLALYRAGIRNALATCGTALTDGHISLLKKHAQRVCLLFDGDNAGRKATVRAME
LCLEQALPVYVINLPQGEDPDSLLQKHGVEAFNQRVAAAKPAFEQFLHWLLAKTPADSIDQRVRLMDEIIPRFQRIKDPV
ERDLYQKEICRLLGVDLQAFRRRLAGQRDQQQPVAQRQTATKPVTQTIARDSTQETMLGLLAAYPEARLEIRTLDLQQLF
DQQHLPLAQAILAAGEGEHDGPLWPEVLARLEQEQQKELVARLLIQDQHLAGIDWRAALRQCLKRQKQGETRGLKQIALR
LATLPEDSEEYQELLKTADQLRNQKSAL
>Mature_588_residues
MNRNDTVREVAERLSIAEIIGEYVSLKRSGSNFLGLCPFHGEKTPSFNVNPAREIFHCFGCGAGGDIFSFVMKIEGISFP
EALRKLAARAGVAIEDRPLTDAEKQLKTERDQQRAIMLLTAQHYRETLTRQPEGAVARSYLQEREVDPETAAAYGLGYAP
ERRDTLIQLLRAKGLPLEQAEQLGIIRRGDRGWYDLLHHRLIFPIRDKQGQPIGFAGRVLDNSLPKYINSPESPLYRKSS
VLFGVDLALRDIRQSGTAIIVEGYFDHLALYRAGIRNALATCGTALTDGHISLLKKHAQRVCLLFDGDNAGRKATVRAME
LCLEQALPVYVINLPQGEDPDSLLQKHGVEAFNQRVAAAKPAFEQFLHWLLAKTPADSIDQRVRLMDEIIPRFQRIKDPV
ERDLYQKEICRLLGVDLQAFRRRLAGQRDQQQPVAQRQTATKPVTQTIARDSTQETMLGLLAAYPEARLEIRTLDLQQLF
DQQHLPLAQAILAAGEGEHDGPLWPEVLARLEQEQQKELVARLLIQDQHLAGIDWRAALRQCLKRQKQGETRGLKQIALR
LATLPEDSEEYQELLKTADQLRNQKSAL

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=474, Percent_Identity=35.0210970464135, Blast_Score=262, Evalue=4e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007693
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 66323; Mature: 66323

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRNDTVREVAERLSIAEIIGEYVSLKRSGSNFLGLCPFHGEKTPSFNVNPAREIFHCFG
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHC
CGAGGDIFSFVMKIEGISFPEALRKLAARAGVAIEDRPLTDAEKQLKTERDQQRAIMLLT
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AQHYRETLTRQPEGAVARSYLQEREVDPETAAAYGLGYAPERRDTLIQLLRAKGLPLEQA
HHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHH
EQLGIIRRGDRGWYDLLHHRLIFPIRDKQGQPIGFAGRVLDNSLPKYINSPESPLYRKSS
HHCCCCCCCCCHHHHHHHHHHCCEEECCCCCCCCHHHHHHHHCCHHHHCCCCCCCHHHCC
VLFGVDLALRDIRQSGTAIIVEGYFDHLALYRAGIRNALATCGTALTDGHISLLKKHAQR
HHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
VCLLFDGDNAGRKATVRAMELCLEQALPVYVINLPQGEDPDSLLQKHGVEAFNQRVAAAK
EEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCHHHHHHHHHHCC
PAFEQFLHWLLAKTPADSIDQRVRLMDEIIPRFQRIKDPVERDLYQKEICRLLGVDLQAF
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHH
RRRLAGQRDQQQPVAQRQTATKPVTQTIARDSTQETMLGLLAAYPEARLEIRTLDLQQLF
HHHHCCCCCCCCHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHCCHHHEEEEEEHHHHHH
DQQHLPLAQAILAAGEGEHDGPLWPEVLARLEQEQQKELVARLLIQDQHLAGIDWRAALR
CCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
QCLKRQKQGETRGLKQIALRLATLPEDSEEYQELLKTADQLRNQKSAL
HHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNRNDTVREVAERLSIAEIIGEYVSLKRSGSNFLGLCPFHGEKTPSFNVNPAREIFHCFG
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHC
CGAGGDIFSFVMKIEGISFPEALRKLAARAGVAIEDRPLTDAEKQLKTERDQQRAIMLLT
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AQHYRETLTRQPEGAVARSYLQEREVDPETAAAYGLGYAPERRDTLIQLLRAKGLPLEQA
HHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHH
EQLGIIRRGDRGWYDLLHHRLIFPIRDKQGQPIGFAGRVLDNSLPKYINSPESPLYRKSS
HHCCCCCCCCCHHHHHHHHHHCCEEECCCCCCCCHHHHHHHHCCHHHHCCCCCCCHHHCC
VLFGVDLALRDIRQSGTAIIVEGYFDHLALYRAGIRNALATCGTALTDGHISLLKKHAQR
HHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
VCLLFDGDNAGRKATVRAMELCLEQALPVYVINLPQGEDPDSLLQKHGVEAFNQRVAAAK
EEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCHHHHHHHHHHCC
PAFEQFLHWLLAKTPADSIDQRVRLMDEIIPRFQRIKDPVERDLYQKEICRLLGVDLQAF
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHH
RRRLAGQRDQQQPVAQRQTATKPVTQTIARDSTQETMLGLLAAYPEARLEIRTLDLQQLF
HHHHCCCCCCCCHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHCCHHHEEEEEEHHHHHH
DQQHLPLAQAILAAGEGEHDGPLWPEVLARLEQEQQKELVARLLIQDQHLAGIDWRAALR
CCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
QCLKRQKQGETRGLKQIALRLATLPEDSEEYQELLKTADQLRNQKSAL
HHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]