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The map label for this gene is hemA

Identifier: 189423574

GI number: 189423574

Start: 478585

End: 479889

Strand: Reverse

Name: hemA

Synonym: Glov_0503

Alternate gene names: 189423574

Gene position: 479889-478585 (Counterclockwise)

Preceding gene: 189423575

Following gene: 189423573

Centisome position: 12.25

GC content: 59.54

Gene sequence:

>1305_bases
ATGAACATCATCGTTGTCGGCCTGTCCCACAAGACAGCGTCGGTTGATATCAGAGAGAAGGTCGCCTTCTCACCCAACAG
CATCGAAAAGCCCCTGCACGAGCTGGTTAATCTGGACGGCATTGTCGAAGGGATCATCGTCTCCACCTGCAACCGGGTCG
AAATCTACGCCACCACCCGCGACATTGCCGGCGGCATCGCCCGCATCCGCCGCTTTATGGCCGAGTACCACCACCTGGCC
CACGACCTGCTCGAACCGCATCTCTACAGCTACCATGGTGAAGAGGCGATCCGCCACGTCTTCAGGGTGGCCTCGTCACT
GGATTCAATGGTGGTTGGTGAGCCGCAGATCCTGGGACAGATCAAGACCTCCTACGGCTACGCCGCCGAGTACAAGAGTT
CCGGCATCATCCTGAACCGTTTCCTGCACAAGGCGTTTTCCGTTGCCAAGCGGGTACGTACCGAGACCAGAATCGCCTCG
TCAGCGGTATCGGTCTCCTTTGCCGCAGTTGAGCTGGCCCGCAAGATCTTCGGCAATCTGACCGACAAGACCGTGCTGTT
GATCGGTGCCGGCGAGATGTGCGAACTGGCAGCCCGCCACTTCCTGACCAACGGCGCCAAGGGGGTGATGGTCACCAACC
GGACCTTTGAGCGTGCCCAGCGCCTTGCTGAGGAGTTCGGCGGCGAGGCGATCCCGTTTGACGAGCTGTTCCTGCATCTG
CACAAGGCGGATATTGTCCTGACCTCCACCGGTGCGCCCCACGCCATCATCACACCGCCTGACCTGGAAGAGGTGATCAA
GCGCCGCAGGATGCGGCCGATGTTCCTGATCGACATTGCCGTACCCCGCGATATCGATCCGGCAGTCAACGAGATGGATG
CGGTCTACCTGTATGATATGGATGACCTGCAGCAGGTGGTGGCTGCCAACCTTGAGGGACGCAAGCAGGAGGCAGACAAG
GCCGAGGCGATCATTGCCGAGGAGATCATCCAGTTCTACAAATGGGTGGCCACCCTTGAGGTCACCCCCACCATCGTTGC
CCTGCGCAACCGCTTTGAAGAGCTGCGCAAAGCGGAACTGGAACGCACCCTGGCCGGCTGGAAGGATGCCCCGCCGGACG
CGGAAAAACGGCTGGAAGCCCTGACCAGCGCCTTTATGAACAAGCTGCTGCACCAGCCCACCACCGTACTCAAAAAGGCA
GGCCAGGGGAATCGCACCGATCTGTACCTGGATGCCCTGCGAGCGCTGTTTGATCTGGAACTGGGCGGCACCGACACGAA
TGAATCACTTGAATTGGAAGAATAA

Upstream 100 bases:

>100_bases
CCATCCTGACCGTCATCGGCTTTCTGGTCATGCTCTTTACCTTCCTCGGGGTAAACCTCTGGCTCCCCGGCCTGCATAGC
TACACCTAAGGGACGGCCGT

Downstream 100 bases:

>100_bases
CCGGAGATAAAGGAGATTGTACACATGGCACCTAAACGCCTCCGCATAGGCACCCGCGCCAGCCAGCTGGCCCTCTGGCA
GGCCAACTGGACCAAATCGG

Product: glutamyl-tRNA reductase

Products: NA

Alternate protein names: GluTR

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MNIIVVGLSHKTASVDIREKVAFSPNSIEKPLHELVNLDGIVEGIIVSTCNRVEIYATTRDIAGGIARIRRFMAEYHHLA
HDLLEPHLYSYHGEEAIRHVFRVASSLDSMVVGEPQILGQIKTSYGYAAEYKSSGIILNRFLHKAFSVAKRVRTETRIAS
SAVSVSFAAVELARKIFGNLTDKTVLLIGAGEMCELAARHFLTNGAKGVMVTNRTFERAQRLAEEFGGEAIPFDELFLHL
HKADIVLTSTGAPHAIITPPDLEEVIKRRRMRPMFLIDIAVPRDIDPAVNEMDAVYLYDMDDLQQVVAANLEGRKQEADK
AEAIIAEEIIQFYKWVATLEVTPTIVALRNRFEELRKAELERTLAGWKDAPPDAEKRLEALTSAFMNKLLHQPTTVLKKA
GQGNRTDLYLDALRALFDLELGGTDTNESLELEE

Sequences:

>Translated_434_residues
MNIIVVGLSHKTASVDIREKVAFSPNSIEKPLHELVNLDGIVEGIIVSTCNRVEIYATTRDIAGGIARIRRFMAEYHHLA
HDLLEPHLYSYHGEEAIRHVFRVASSLDSMVVGEPQILGQIKTSYGYAAEYKSSGIILNRFLHKAFSVAKRVRTETRIAS
SAVSVSFAAVELARKIFGNLTDKTVLLIGAGEMCELAARHFLTNGAKGVMVTNRTFERAQRLAEEFGGEAIPFDELFLHL
HKADIVLTSTGAPHAIITPPDLEEVIKRRRMRPMFLIDIAVPRDIDPAVNEMDAVYLYDMDDLQQVVAANLEGRKQEADK
AEAIIAEEIIQFYKWVATLEVTPTIVALRNRFEELRKAELERTLAGWKDAPPDAEKRLEALTSAFMNKLLHQPTTVLKKA
GQGNRTDLYLDALRALFDLELGGTDTNESLELEE
>Mature_434_residues
MNIIVVGLSHKTASVDIREKVAFSPNSIEKPLHELVNLDGIVEGIIVSTCNRVEIYATTRDIAGGIARIRRFMAEYHHLA
HDLLEPHLYSYHGEEAIRHVFRVASSLDSMVVGEPQILGQIKTSYGYAAEYKSSGIILNRFLHKAFSVAKRVRTETRIAS
SAVSVSFAAVELARKIFGNLTDKTVLLIGAGEMCELAARHFLTNGAKGVMVTNRTFERAQRLAEEFGGEAIPFDELFLHL
HKADIVLTSTGAPHAIITPPDLEEVIKRRRMRPMFLIDIAVPRDIDPAVNEMDAVYLYDMDDLQQVVAANLEGRKQEADK
AEAIIAEEIIQFYKWVATLEVTPTIVALRNRFEELRKAELERTLAGWKDAPPDAEKRLEALTSAFMNKLLHQPTTVLKKA
GQGNRTDLYLDALRALFDLELGGTDTNESLELEE

Specific function: Catalyzes the NADPH-dependent reduction of glutamyl- tRNA(Glu) to glutamate 1-semialdehyde (GSA)

COG id: COG0373

COG function: function code H; Glutamyl-tRNA reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamyl-tRNA reductase family

Homologues:

Organism=Escherichia coli, GI1787461, Length=416, Percent_Identity=40.625, Blast_Score=308, Evalue=4e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM1_GEOLS (B3E2H8)

Other databases:

- EMBL:   CP001089
- RefSeq:   YP_001950751.1
- ProteinModelPortal:   B3E2H8
- GeneID:   6367436
- GenomeReviews:   CP001089_GR
- KEGG:   glo:Glov_0503
- HOGENOM:   HBG732626
- OMA:   LLIDIAN
- ProtClustDB:   PRK00045
- GO:   GO:0005737
- HAMAP:   MF_00087
- InterPro:   IPR000343
- InterPro:   IPR015896
- InterPro:   IPR015895
- InterPro:   IPR016040
- InterPro:   IPR018214
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000445
- TIGRFAMs:   TIGR01035

Pfam domain/function: PF00745 GlutR_dimer; PF05201 GlutR_N; PF01488 Shikimate_DH; SSF69075 4pyrrol_synth_GluRdtase_C; SSF69742 GlutR

EC number: =1.2.1.70

Molecular weight: Translated: 48472; Mature: 48472

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00747 GLUTR

Important sites: ACT_SITE 50-50 BINDING 109-109 BINDING 120-120

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIIVVGLSHKTASVDIREKVAFSPNSIEKPLHELVNLDGIVEGIIVSTCNRVEIYATTR
CCEEEEEECCCCCCCCHHHHHCCCCCHHHHHHHHHHCHHHHHHHHHHHCCCCEEEEEEHH
DIAGGIARIRRFMAEYHHLAHDLLEPHLYSYHGEEAIRHVFRVASSLDSMVVGEPQILGQ
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHH
IKTSYGYAAEYKSSGIILNRFLHKAFSVAKRVRTETRIASSAVSVSFAAVELARKIFGNL
HHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TDKTVLLIGAGEMCELAARHFLTNGAKGVMVTNRTFERAQRLAEEFGGEAIPFDELFLHL
CCCEEEEEECCHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHCCCCCCHHHHHHHH
HKADIVLTSTGAPHAIITPPDLEEVIKRRRMRPMFLIDIAVPRDIDPAVNEMDAVYLYDM
HHCCEEEECCCCCCEEECCCCHHHHHHHHCCCCEEEEEEECCCCCCHHHHCCCEEEEECH
DDLQQVVAANLEGRKQEADKAEAIIAEEIIQFYKWVATLEVTPTIVALRNRFEELRKAEL
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
ERTLAGWKDAPPDAEKRLEALTSAFMNKLLHQPTTVLKKAGQGNRTDLYLDALRALFDLE
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCHHHHHHHHHHHHHC
LGGTDTNESLELEE
CCCCCCCCCCCCCC
>Mature Secondary Structure
MNIIVVGLSHKTASVDIREKVAFSPNSIEKPLHELVNLDGIVEGIIVSTCNRVEIYATTR
CCEEEEEECCCCCCCCHHHHHCCCCCHHHHHHHHHHCHHHHHHHHHHHCCCCEEEEEEHH
DIAGGIARIRRFMAEYHHLAHDLLEPHLYSYHGEEAIRHVFRVASSLDSMVVGEPQILGQ
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHH
IKTSYGYAAEYKSSGIILNRFLHKAFSVAKRVRTETRIASSAVSVSFAAVELARKIFGNL
HHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TDKTVLLIGAGEMCELAARHFLTNGAKGVMVTNRTFERAQRLAEEFGGEAIPFDELFLHL
CCCEEEEEECCHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHCCCCCCHHHHHHHH
HKADIVLTSTGAPHAIITPPDLEEVIKRRRMRPMFLIDIAVPRDIDPAVNEMDAVYLYDM
HHCCEEEECCCCCCEEECCCCHHHHHHHHCCCCEEEEEEECCCCCCHHHHCCCEEEEECH
DDLQQVVAANLEGRKQEADKAEAIIAEEIIQFYKWVATLEVTPTIVALRNRFEELRKAEL
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
ERTLAGWKDAPPDAEKRLEALTSAFMNKLLHQPTTVLKKAGQGNRTDLYLDALRALFDLE
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCHHHHHHHHHHHHHC
LGGTDTNESLELEE
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA