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The map label for this gene is capI [H]

Identifier: 189423550

GI number: 189423550

Start: 454533

End: 455546

Strand: Reverse

Name: capI [H]

Synonym: Glov_0479

Alternate gene names: 189423550

Gene position: 455546-454533 (Counterclockwise)

Preceding gene: 189423551

Following gene: 189423549

Centisome position: 11.63

GC content: 55.33

Gene sequence:

>1014_bases
ATGCGGATTCTTGTTACCGGCGCAGCCGGATTCATCGGTTTTCACCTCTGCCGGCGCCTGCTGGCACGGGGCGATGCGGT
CCTGGGGCTGGACAATCTGAATAGCTACTATGATGTCAGCCTGAAACAGGCCCGGCTTGCACAGCTACAGCCCCACCCCG
GCTTCAGCTTTGTACAGGCAGACCTGGCAGACCGTCCTGCAATGGAACAACTGTTCAGCGTGGAGCAGTTCGATATTGTT
ATTCATCTGGCAGCCCAGGCCGGTGTCCGATACTCGCTTGAGAACCCCCATGCCTATGTTGACAGCAACCTGACCGGCTT
TATGAACATCCTGGAAGGCTGCCGTCATACTGGCGTCAAACATCTGGTCTTTGCCTCTTCAAGTTCGGTCTATGGCGCCA
ACACCGCCATCCCCTTTTCCATTCACCAGAATGTCGATCACCCTGTTTCTCTCTATGCGGCCACCAAAAAGGCCAATGAG
CTGATGGCCCATAGCTACGCAAGCCTCTTCAATCTGCCGGTTACCGGCTTGCGGCTGTTCACGGTTTACGGCCCCTGGGG
CAGACCGGATATGGCCTATTTTTCCTTTACCAGGGCCATCCTGGAAGGCAGACCGATCAATATCTTCAACCGGGGCAGGA
TGCAGCGGGATTTTACCTATGTGGATGACATCGTAGAGGGTATTGTCCGCATAGCTGAAAAACCTGCAGAGAAAAACCCA
ACCTGGTCCGGTACGCAGCCTGATCCCGGCACCAGCTTTGCCCCCTATCGAATCTACAACATCGGCAACAACAAACCGGT
TGAGCTGTTGCAGTTCATAGAGATTCTGGAACAACAGCTGGGGATAACCGCGCAGAAAAACTTTCTACCGATGCAGGCTG
GCGACGTGCCTGCCACCTTTGCCGATATCGATGAGCTGGCTGCCGCGACCGGTTTCAGGCCTGCCACCTCGCTGGAGGAC
GGTATTGCACGCTTTGTTGCATGGTTCCGCTCGTACTACAGCCATCCCGCATAA

Upstream 100 bases:

>100_bases
TACAGCTGCTACTGGAGACCCGTGGCCAGGAACATGCCCACGAGGTACTCGGCCTGCTTGCAAAACGCGGCTATTCTGTC
ACAACGGAGCAGTAACCATT

Downstream 100 bases:

>100_bases
ATCGCATTGAAAGGAAGAAACAGCATGAACCGAAAAGGCATTATCCTGGCTGGCGGAGCCGGCACCCGTCTGCATCCGGC
AACCCTGGCCGTATCGAAAC

Product: NAD-dependent epimerase/dehydratase

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MRILVTGAAGFIGFHLCRRLLARGDAVLGLDNLNSYYDVSLKQARLAQLQPHPGFSFVQADLADRPAMEQLFSVEQFDIV
IHLAAQAGVRYSLENPHAYVDSNLTGFMNILEGCRHTGVKHLVFASSSSVYGANTAIPFSIHQNVDHPVSLYAATKKANE
LMAHSYASLFNLPVTGLRLFTVYGPWGRPDMAYFSFTRAILEGRPINIFNRGRMQRDFTYVDDIVEGIVRIAEKPAEKNP
TWSGTQPDPGTSFAPYRIYNIGNNKPVELLQFIEILEQQLGITAQKNFLPMQAGDVPATFADIDELAAATGFRPATSLED
GIARFVAWFRSYYSHPA

Sequences:

>Translated_337_residues
MRILVTGAAGFIGFHLCRRLLARGDAVLGLDNLNSYYDVSLKQARLAQLQPHPGFSFVQADLADRPAMEQLFSVEQFDIV
IHLAAQAGVRYSLENPHAYVDSNLTGFMNILEGCRHTGVKHLVFASSSSVYGANTAIPFSIHQNVDHPVSLYAATKKANE
LMAHSYASLFNLPVTGLRLFTVYGPWGRPDMAYFSFTRAILEGRPINIFNRGRMQRDFTYVDDIVEGIVRIAEKPAEKNP
TWSGTQPDPGTSFAPYRIYNIGNNKPVELLQFIEILEQQLGITAQKNFLPMQAGDVPATFADIDELAAATGFRPATSLED
GIARFVAWFRSYYSHPA
>Mature_337_residues
MRILVTGAAGFIGFHLCRRLLARGDAVLGLDNLNSYYDVSLKQARLAQLQPHPGFSFVQADLADRPAMEQLFSVEQFDIV
IHLAAQAGVRYSLENPHAYVDSNLTGFMNILEGCRHTGVKHLVFASSSSVYGANTAIPFSIHQNVDHPVSLYAATKKANE
LMAHSYASLFNLPVTGLRLFTVYGPWGRPDMAYFSFTRAILEGRPINIFNRGRMQRDFTYVDDIVEGIVRIAEKPAEKNP
TWSGTQPDPGTSFAPYRIYNIGNNKPVELLQFIEILEQQLGITAQKNFLPMQAGDVPATFADIDELAAATGFRPATSLED
GIARFVAWFRSYYSHPA

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=339, Percent_Identity=26.5486725663717, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI42516563, Length=345, Percent_Identity=25.7971014492754, Blast_Score=111, Evalue=9e-25,
Organism=Homo sapiens, GI56237023, Length=351, Percent_Identity=27.6353276353276, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI56118217, Length=351, Percent_Identity=27.6353276353276, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI189083684, Length=351, Percent_Identity=27.6353276353276, Blast_Score=99, Evalue=5e-21,
Organism=Escherichia coli, GI1786974, Length=340, Percent_Identity=28.5294117647059, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI1788353, Length=355, Percent_Identity=25.6338028169014, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI48994969, Length=352, Percent_Identity=25.8522727272727, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788589, Length=253, Percent_Identity=28.4584980237154, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71982035, Length=357, Percent_Identity=30.5322128851541, Blast_Score=111, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI71982038, Length=357, Percent_Identity=29.4117647058824, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17539532, Length=340, Percent_Identity=27.6470588235294, Blast_Score=107, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17568069, Length=341, Percent_Identity=25.5131964809384, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI115532424, Length=349, Percent_Identity=23.2091690544413, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6319493, Length=347, Percent_Identity=27.3775216138329, Blast_Score=83, Evalue=5e-17,
Organism=Drosophila melanogaster, GI21356223, Length=341, Percent_Identity=26.9794721407625, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI19923002, Length=321, Percent_Identity=26.1682242990654, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 37410; Mature: 37410

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILVTGAAGFIGFHLCRRLLARGDAVLGLDNLNSYYDVSLKQARLAQLQPHPGFSFVQA
CEEEEECCHHHHHHHHHHHHHHCCCEEEEECCCCCCEECHHHHHHHHHCCCCCCHHHHHH
DLADRPAMEQLFSVEQFDIVIHLAAQAGVRYSLENPHAYVDSNLTGFMNILEGCRHTGVK
HHCCCHHHHHHHHHHHHHHEEEEEHHCCCEEECCCCCEEECCCHHHHHHHHHHHHHCCCE
HLVFASSSSVYGANTAIPFSIHQNVDHPVSLYAATKKANELMAHSYASLFNLPVTGLRLF
EEEEECCCCCCCCCCEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
TVYGPWGRPDMAYFSFTRAILEGRPINIFNRGRMQRDFTYVDDIVEGIVRIAEKPAEKNP
EEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
TWSGTQPDPGTSFAPYRIYNIGNNKPVELLQFIEILEQQLGITAQKNFLPMQAGDVPATF
CCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHH
ADIDELAAATGFRPATSLEDGIARFVAWFRSYYSHPA
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRILVTGAAGFIGFHLCRRLLARGDAVLGLDNLNSYYDVSLKQARLAQLQPHPGFSFVQA
CEEEEECCHHHHHHHHHHHHHHCCCEEEEECCCCCCEECHHHHHHHHHCCCCCCHHHHHH
DLADRPAMEQLFSVEQFDIVIHLAAQAGVRYSLENPHAYVDSNLTGFMNILEGCRHTGVK
HHCCCHHHHHHHHHHHHHHEEEEEHHCCCEEECCCCCEEECCCHHHHHHHHHHHHHCCCE
HLVFASSSSVYGANTAIPFSIHQNVDHPVSLYAATKKANELMAHSYASLFNLPVTGLRLF
EEEEECCCCCCCCCCEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
TVYGPWGRPDMAYFSFTRAILEGRPINIFNRGRMQRDFTYVDDIVEGIVRIAEKPAEKNP
EEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
TWSGTQPDPGTSFAPYRIYNIGNNKPVELLQFIEILEQQLGITAQKNFLPMQAGDVPATF
CCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHH
ADIDELAAATGFRPATSLEDGIARFVAWFRSYYSHPA
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]