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The map label for this gene is nemA [H]

Identifier: 189353923

GI number: 189353923

Start: 2237955

End: 2239070

Strand: Direct

Name: nemA [H]

Synonym: BMULJ_05176

Alternate gene names: 189353923

Gene position: 2237955-2239070 (Clockwise)

Preceding gene: 189353922

Following gene: 189353924

Centisome position: 90.49

GC content: 70.07

Gene sequence:

>1116_bases
ATGACGCAGGACCCGCTTTTCCAACCGCTTCGCCTCGGCGCGCTGACGCTGCCGAATCGCATCGTGATGCCGCCGATGAC
GCGCTCGCGCGCGAGCCAGCCCGGCGACGTCGCGAACGAACTGATGGCCGCGTACTACGCGCAGCGCGCGAGTGCGGGGC
TGATCGTCAGCGAAGGCACCTACATCGCACCGCTCGGCAAGGGCTACGCATGGACGCCCGGCATTCACACGCCCGCGCAG
GTCGCCGGCTGGCGCAAGGTGACGGACGCCGTGCATGCCGCGCAGGGCCGCATCTTCGCGCAGCTCTGGCACGTCGGGCG
GCTCAGCCACACGAGCCTGCTCGGCGGCCGGCAGCCCGTGTCGTCGTCGCCGCTGCAGGCGAACGGCGTGAACGTCTTCA
TCGCCGGCGAAGACGGCAGCACGCCCGGCTTCGTGCAGGCGTCCGAGCCGCGCGCGCTGACGATCGACGAGATCCGCGAC
ATCGTCGAGCAGTATCGCGTCGCCGCGCGCAACGCGATCGAAGCGGGCTTCGATGGCGTCGAGCTGCACGGCGCGAACGG
CTATCTCGTCAACCAGTTCATCGATTCGAACGCGAACACGCGCACCGACGCGTACGGCGGCTCGCTCGAGAACCGGCTGC
GTTTCCTGCGCGAAGTCACGCAGGCGCTGATCGACGGCACCGGCGACGCATCGCGCGTCGGCGTGCGGCTCGCGCCGCTG
ACCACGCTGAACGGCTGCGTCGACGACGATCCGGAAACGACCTATCTCGCGGCCGCCAAGCTGCTCGGCGAACTCGGGAT
CGGCTATCTGCACATCGCGGAAGCCGACTGGGACGACGCGCCGCTGATGCCGGTCGCGTTCAAGCAGCAGTTGCGCGCCG
CGTTCCCGGGCGTGTTGATCTACGCCGGCAAATACACGGCCGAGCGCGCCCGCGAAGCGATCGCGGCCGGCTGGGCGGAC
TTGATCGCGTTCGGCCGGCCATTCGTCGCGAATCCGGACCTGCCTGAGCGCCTGCGTACGGGCGCGCCGCTCGCGCTGCA
CGACCGCAACACGCTGTTCGGCGGCGGTGCGCGAGGGCTGACCGACTATCCGACGCTCGCGCAAGCGGCCGCGTAA

Upstream 100 bases:

>100_bases
TCGATCCGGCCGGCCTCGCGCCGAAGTGGGACTGAACTCGGCCCGTTATCGAATCGACCCGTCCGCGGCCGTGCGCCGCG
GCATCCGACAGGACACCCTC

Downstream 100 bases:

>100_bases
CGATCGCGAGCGCCACGACATGGACGCCATGCGCGCTGCATGAACGCCATGCGTGCGGCGCTCGCGCCGATTCCGGCCGA
ACCGTGGGGCGCGCTGACGG

Product: Old Yellow Enzyme family NADH:flavin oxidoreductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MTQDPLFQPLRLGALTLPNRIVMPPMTRSRASQPGDVANELMAAYYAQRASAGLIVSEGTYIAPLGKGYAWTPGIHTPAQ
VAGWRKVTDAVHAAQGRIFAQLWHVGRLSHTSLLGGRQPVSSSPLQANGVNVFIAGEDGSTPGFVQASEPRALTIDEIRD
IVEQYRVAARNAIEAGFDGVELHGANGYLVNQFIDSNANTRTDAYGGSLENRLRFLREVTQALIDGTGDASRVGVRLAPL
TTLNGCVDDDPETTYLAAAKLLGELGIGYLHIAEADWDDAPLMPVAFKQQLRAAFPGVLIYAGKYTAERAREAIAAGWAD
LIAFGRPFVANPDLPERLRTGAPLALHDRNTLFGGGARGLTDYPTLAQAAA

Sequences:

>Translated_371_residues
MTQDPLFQPLRLGALTLPNRIVMPPMTRSRASQPGDVANELMAAYYAQRASAGLIVSEGTYIAPLGKGYAWTPGIHTPAQ
VAGWRKVTDAVHAAQGRIFAQLWHVGRLSHTSLLGGRQPVSSSPLQANGVNVFIAGEDGSTPGFVQASEPRALTIDEIRD
IVEQYRVAARNAIEAGFDGVELHGANGYLVNQFIDSNANTRTDAYGGSLENRLRFLREVTQALIDGTGDASRVGVRLAPL
TTLNGCVDDDPETTYLAAAKLLGELGIGYLHIAEADWDDAPLMPVAFKQQLRAAFPGVLIYAGKYTAERAREAIAAGWAD
LIAFGRPFVANPDLPERLRTGAPLALHDRNTLFGGGARGLTDYPTLAQAAA
>Mature_370_residues
TQDPLFQPLRLGALTLPNRIVMPPMTRSRASQPGDVANELMAAYYAQRASAGLIVSEGTYIAPLGKGYAWTPGIHTPAQV
AGWRKVTDAVHAAQGRIFAQLWHVGRLSHTSLLGGRQPVSSSPLQANGVNVFIAGEDGSTPGFVQASEPRALTIDEIRDI
VEQYRVAARNAIEAGFDGVELHGANGYLVNQFIDSNANTRTDAYGGSLENRLRFLREVTQALIDGTGDASRVGVRLAPLT
TLNGCVDDDPETTYLAAAKLLGELGIGYLHIAEADWDDAPLMPVAFKQQLRAAFPGVLIYAGKYTAERAREAIAAGWADL
IAFGRPFVANPDLPERLRTGAPLALHDRNTLFGGGARGLTDYPTLAQAAA

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=369, Percent_Identity=48.5094850948509, Blast_Score=327, Evalue=1e-90,
Organism=Escherichia coli, GI1789463, Length=357, Percent_Identity=32.2128851540616, Blast_Score=130, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17565138, Length=317, Percent_Identity=29.6529968454259, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17559802, Length=259, Percent_Identity=30.1158301158301, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17559804, Length=266, Percent_Identity=31.5789473684211, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI72001454, Length=259, Percent_Identity=31.2741312741313, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17564188, Length=255, Percent_Identity=30.5882352941176, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17540738, Length=222, Percent_Identity=32.4324324324324, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17566914, Length=224, Percent_Identity=26.3392857142857, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6325086, Length=386, Percent_Identity=37.8238341968912, Blast_Score=228, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6321973, Length=394, Percent_Identity=36.8020304568528, Blast_Score=218, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39640; Mature: 39509

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQDPLFQPLRLGALTLPNRIVMPPMTRSRASQPGDVANELMAAYYAQRASAGLIVSEGT
CCCCCCCHHHHHCEEECCCEEECCCCHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEECCC
YIAPLGKGYAWTPGIHTPAQVAGWRKVTDAVHAAQGRIFAQLWHVGRLSHTSLLGGRQPV
EEEECCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCC
SSSPLQANGVNVFIAGEDGSTPGFVQASEPRALTIDEIRDIVEQYRVAARNAIEAGFDGV
CCCCCCCCCEEEEEECCCCCCCCEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCE
ELHGANGYLVNQFIDSNANTRTDAYGGSLENRLRFLREVTQALIDGTGDASRVGVRLAPL
EEECCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHCCEEEECC
TTLNGCVDDDPETTYLAAAKLLGELGIGYLHIAEADWDDAPLMPVAFKQQLRAAFPGVLI
HHHCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCEEE
YAGKYTAERAREAIAAGWADLIAFGRPFVANPDLPERLRTGAPLALHDRNTLFGGGARGL
EECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCEEEECCCCEECCCCCCC
TDYPTLAQAAA
CCCCCHHHHCC
>Mature Secondary Structure 
TQDPLFQPLRLGALTLPNRIVMPPMTRSRASQPGDVANELMAAYYAQRASAGLIVSEGT
CCCCCCHHHHHCEEECCCEEECCCCHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEECCC
YIAPLGKGYAWTPGIHTPAQVAGWRKVTDAVHAAQGRIFAQLWHVGRLSHTSLLGGRQPV
EEEECCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCC
SSSPLQANGVNVFIAGEDGSTPGFVQASEPRALTIDEIRDIVEQYRVAARNAIEAGFDGV
CCCCCCCCCEEEEEECCCCCCCCEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCE
ELHGANGYLVNQFIDSNANTRTDAYGGSLENRLRFLREVTQALIDGTGDASRVGVRLAPL
EEECCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHCCEEEECC
TTLNGCVDDDPETTYLAAAKLLGELGIGYLHIAEADWDDAPLMPVAFKQQLRAAFPGVLI
HHHCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCEEE
YAGKYTAERAREAIAAGWADLIAFGRPFVANPDLPERLRTGAPLALHDRNTLFGGGARGL
EECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCEEEECCCCEECCCCCCC
TDYPTLAQAAA
CCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]