BacMap

Definition	Burkholderia multivorans ATCC 17616 chromosome 2, complete genome.
Accession	NC_010805
Length	2,473,162

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The map label for this gene is dkgB [H]

Identifier: 189353922

GI number: 189353922

Start: 2237083

End: 2237889

Strand: Direct

Name: dkgB [H]

Synonym: BMULJ_05175

Alternate gene names: 189353922

Gene position: 2237083-2237889 (Clockwise)

Preceding gene: 189353920

Following gene: 189353923

Centisome position: 90.45

GC content: 66.67

Gene sequence:

>807_bases
ATGAACCCCATTCCCGCATTCGGCCTCGGCACGTATCGCCTGAAGGGCCAGGTCGTCATCGACTCGGTCCGCAACGGCCT
CGAACTCGGCTACCGTGCGATCGACACCGCGCAGATCTACGGCAACGAAGCCGAAGTCGGTGAGGCGATCGCCGCGTCGG
GCGTCAGCCGCGACGCGCTGTTCCTGACGACGAAGATCTGGGTCGACAACTATGCGCCCGACAAGCTCGTGCCGAGCCTC
GAGGACAGCCTGCGCAAGCTGCGCACCGACTACGTCGACCTGACGCTGATCCACTGGCCGGCGCCCGACAACGGCGTGCC
GCTCGCGACGTTCATGACCGCGCTCGCCGACGCGCAGGCGAAGGGCCTCACGCGCCGGATCGGCATCTCGAACTTCAACA
TCGCGCTGACGAAGGAGGCGATCGCGGCGGTCGGCAAGGACGCGATCGCGACGAACCAGATCGAACTGAGCCCGTATCTG
CAGAACCGCAAGCTCGTCGAATTCCTGAACGCCGAAGGCATCCACGTGACGTCGTACATGACGCTCGCCTACGGCAAGGT
GCTCGGCGACCCGGTGCTCGGCGCGATCGCGAAGCGCCACGATGCGACGCCCGCGCAGGTCGCGCTCGCCTGGGCGATGC
AGCTCGGCTACTCGGTGATTCCGTCGTCGACGAAGCGCGAAAACCTCGCGAGCAACCTGCTCGCGCAAACGCTGCGCTTG
ACCGACGACGACATGGCGCAGATCGCCGCGCTTGAGCGCAACGGCCGCGAAGTCGATCCGGCCGGCCTCGCGCCGAAGTG
GGACTGA

Upstream 100 bases:

>100_bases
GAATCAAATATTTTTGTCCGCTAACGGCGTTTATCTCACGAGTCCGACGGACCACAATGATTCCCGTAGCGACACCGTTC
CATCCATCCAGGAGCCCATG

Downstream 100 bases:

>100_bases
ACTCGGCCCGTTATCGAATCGACCCGTCCGCGGCCGTGCGCCGCGGCATCCGACAGGACACCCTCATGACGCAGGACCCG
CTTTTCCAACCGCTTCGCCT

Product: 2,5-diketo-D-gluconate reductase B

Products: NA

Alternate protein names: 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MNPIPAFGLGTYRLKGQVVIDSVRNGLELGYRAIDTAQIYGNEAEVGEAIAASGVSRDALFLTTKIWVDNYAPDKLVPSL
EDSLRKLRTDYVDLTLIHWPAPDNGVPLATFMTALADAQAKGLTRRIGISNFNIALTKEAIAAVGKDAIATNQIELSPYL
QNRKLVEFLNAEGIHVTSYMTLAYGKVLGDPVLGAIAKRHDATPAQVALAWAMQLGYSVIPSSTKRENLASNLLAQTLRL
TDDDMAQIAALERNGREVDPAGLAPKWD

Sequences:

>Translated_268_residues
MNPIPAFGLGTYRLKGQVVIDSVRNGLELGYRAIDTAQIYGNEAEVGEAIAASGVSRDALFLTTKIWVDNYAPDKLVPSL
EDSLRKLRTDYVDLTLIHWPAPDNGVPLATFMTALADAQAKGLTRRIGISNFNIALTKEAIAAVGKDAIATNQIELSPYL
QNRKLVEFLNAEGIHVTSYMTLAYGKVLGDPVLGAIAKRHDATPAQVALAWAMQLGYSVIPSSTKRENLASNLLAQTLRL
TDDDMAQIAALERNGREVDPAGLAPKWD
>Mature_268_residues
MNPIPAFGLGTYRLKGQVVIDSVRNGLELGYRAIDTAQIYGNEAEVGEAIAASGVSRDALFLTTKIWVDNYAPDKLVPSL
EDSLRKLRTDYVDLTLIHWPAPDNGVPLATFMTALADAQAKGLTRRIGISNFNIALTKEAIAAVGKDAIATNQIELSPYL
QNRKLVEFLNAEGIHVTSYMTLAYGKVLGDPVLGAIAKRHDATPAQVALAWAMQLGYSVIPSSTKRENLASNLLAQTLRL
TDDDMAQIAALERNGREVDPAGLAPKWD

Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) [H]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI310109922, Length=272, Percent_Identity=33.8235294117647, Blast_Score=160, Evalue=9e-40,
Organism=Homo sapiens, GI45446745, Length=289, Percent_Identity=33.2179930795848, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI4503285, Length=289, Percent_Identity=33.2179930795848, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI310109920, Length=289, Percent_Identity=33.2179930795848, Blast_Score=158, Evalue=4e-39,
Organism=Homo sapiens, GI5453543, Length=289, Percent_Identity=32.8719723183391, Blast_Score=156, Evalue=1e-38,
Organism=Homo sapiens, GI24497585, Length=291, Percent_Identity=32.3024054982818, Blast_Score=152, Evalue=2e-37,
Organism=Homo sapiens, GI24497583, Length=287, Percent_Identity=33.4494773519164, Blast_Score=150, Evalue=8e-37,
Organism=Homo sapiens, GI24497577, Length=289, Percent_Identity=32.1799307958478, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI5174391, Length=289, Percent_Identity=32.1799307958478, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI5174695, Length=299, Percent_Identity=35.1170568561873, Blast_Score=148, Evalue=5e-36,
Organism=Homo sapiens, GI223468663, Length=285, Percent_Identity=32.280701754386, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI291291012, Length=291, Percent_Identity=31.6151202749141, Blast_Score=137, Evalue=8e-33,
Organism=Homo sapiens, GI93277124, Length=302, Percent_Identity=30.794701986755, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI300116273, Length=270, Percent_Identity=33.7037037037037, Blast_Score=133, Evalue=1e-31,
Organism=Homo sapiens, GI4502049, Length=285, Percent_Identity=33.6842105263158, Blast_Score=131, Evalue=6e-31,
Organism=Homo sapiens, GI300116271, Length=282, Percent_Identity=32.6241134751773, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI310109924, Length=157, Percent_Identity=33.7579617834395, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI207028673, Length=104, Percent_Identity=39.4230769230769, Blast_Score=84, Evalue=9e-17,
Organism=Homo sapiens, GI310109926, Length=104, Percent_Identity=39.4230769230769, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI1786400, Length=265, Percent_Identity=67.5471698113208, Blast_Score=363, Evalue=1e-102,
Organism=Escherichia coli, GI87082198, Length=259, Percent_Identity=37.0656370656371, Blast_Score=166, Evalue=1e-42,
Organism=Escherichia coli, GI1788081, Length=261, Percent_Identity=30.2681992337165, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI1787674, Length=255, Percent_Identity=26.2745098039216, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI48994888, Length=271, Percent_Identity=27.3062730627306, Blast_Score=69, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17537077, Length=288, Percent_Identity=34.7222222222222, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17552492, Length=269, Percent_Identity=31.5985130111524, Blast_Score=142, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17537075, Length=290, Percent_Identity=34.1379310344828, Blast_Score=141, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17561298, Length=274, Percent_Identity=30.2919708029197, Blast_Score=138, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17550248, Length=283, Percent_Identity=32.5088339222615, Blast_Score=137, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17566692, Length=286, Percent_Identity=31.8181818181818, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17537079, Length=294, Percent_Identity=30.2721088435374, Blast_Score=128, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI71998625, Length=272, Percent_Identity=30.8823529411765, Blast_Score=128, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17538386, Length=264, Percent_Identity=30.6818181818182, Blast_Score=124, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17561300, Length=277, Percent_Identity=30.3249097472924, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17564128, Length=290, Percent_Identity=27.5862068965517, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17562292, Length=269, Percent_Identity=30.1115241635688, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6324694, Length=292, Percent_Identity=33.2191780821918, Blast_Score=150, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6320576, Length=279, Percent_Identity=34.7670250896057, Blast_Score=149, Evalue=5e-37,
Organism=Saccharomyces cerevisiae, GI6321896, Length=304, Percent_Identity=31.9078947368421, Blast_Score=141, Evalue=8e-35,
Organism=Saccharomyces cerevisiae, GI6322556, Length=250, Percent_Identity=31.2, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6319625, Length=282, Percent_Identity=27.6595744680851, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6320079, Length=294, Percent_Identity=29.2517006802721, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24662789, Length=287, Percent_Identity=34.1463414634146, Blast_Score=150, Evalue=7e-37,
Organism=Drosophila melanogaster, GI24644950, Length=303, Percent_Identity=33.3333333333333, Blast_Score=144, Evalue=6e-35,
Organism=Drosophila melanogaster, GI221378297, Length=320, Percent_Identity=31.25, Blast_Score=136, Evalue=1e-32,
Organism=Drosophila melanogaster, GI20129731, Length=289, Percent_Identity=32.8719723183391, Blast_Score=127, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24662785, Length=293, Percent_Identity=34.1296928327645, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24657054, Length=296, Percent_Identity=32.0945945945946, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24662781, Length=291, Percent_Identity=33.6769759450172, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24663317, Length=290, Percent_Identity=30.6896551724138, Blast_Score=120, Evalue=9e-28,
Organism=Drosophila melanogaster, GI45553081, Length=284, Percent_Identity=29.9295774647887, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI21356425, Length=273, Percent_Identity=28.2051282051282, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI281366140, Length=259, Percent_Identity=28.5714285714286, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: =1.1.1.274 [H]

Molecular weight: Translated: 28987; Mature: 28987

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPIPAFGLGTYRLKGQVVIDSVRNGLELGYRAIDTAQIYGNEAEVGEAIAASGVSRDAL
CCCCCCCCCCEEEEECEEEEHHHHHHHHHHHHHEEHHHHCCCHHHHHHHHHHCCCCCCEE
FLTTKIWVDNYAPDKLVPSLEDSLRKLRTDYVDLTLIHWPAPDNGVPLATFMTALADAQA
EEEEEEEECCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHHHHH
KGLTRRIGISNFNIALTKEAIAAVGKDAIATNQIELSPYLQNRKLVEFLNAEGIHVTSYM
HCCHHHCCCCCCEEEEHHHHHHHHCCCHHCCCEEEECHHHCCCHHHHHHCCCCEEEHHHH
TLAYGKVLGDPVLGAIAKRHDATPAQVALAWAMQLGYSVIPSSTKRENLASNLLAQTLRL
HHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHC
TDDDMAQIAALERNGREVDPAGLAPKWD
CCHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNPIPAFGLGTYRLKGQVVIDSVRNGLELGYRAIDTAQIYGNEAEVGEAIAASGVSRDAL
CCCCCCCCCCEEEEECEEEEHHHHHHHHHHHHHEEHHHHCCCHHHHHHHHHHCCCCCCEE
FLTTKIWVDNYAPDKLVPSLEDSLRKLRTDYVDLTLIHWPAPDNGVPLATFMTALADAQA
EEEEEEEECCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHHHHH
KGLTRRIGISNFNIALTKEAIAAVGKDAIATNQIELSPYLQNRKLVEFLNAEGIHVTSYM
HCCHHHCCCCCCEEEEHHHHHHHHCCCHHCCCEEEECHHHCCCHHHHHHCCCCEEEHHHH
TLAYGKVLGDPVLGAIAKRHDATPAQVALAWAMQLGYSVIPSSTKRENLASNLLAQTLRL
HHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHC
TDDDMAQIAALERNGREVDPAGLAPKWD
CCHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]