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The map label for this gene is hipO [H]

Identifier: 189353916

GI number: 189353916

Start: 2230246

End: 2231433

Strand: Reverse

Name: hipO [H]

Synonym: BMULJ_05168

Alternate gene names: 189353916

Gene position: 2231433-2230246 (Counterclockwise)

Preceding gene: 189353921

Following gene: 189353915

Centisome position: 90.23

GC content: 67.93

Gene sequence:

>1188_bases
ATGACCGACACCCGCTTCACCGAAATCGAGGATCTGCTGCCCGCCGCCGGCGCGCTGCGCGAGATCCGCCATCACATCCA
CAGTCACCCCGAACTCGCGTACGAGGAGCACGACACGGCCGCGCTCGTCGCGGACAAGCTCGAGCAGTGGGGCTGGCAGG
TCACGCGCGGCGTCGGCAAGACCGGCGTGGTCGGTACGCTGCGTGTCGGCGACGGCACGCGCAGCATCGGCATCCGCGCC
GACATGGACGCGTTGCCGATCGTCGAGGCGACCGGCCTGCCGTATGCGAGCGCGACGCACGGCAAGATGCATGCATGCGG
TCATGACGGCCACACGACGATGCTGCTCGGCGCCGCGCAACATCTCGCGAAGACGCGCCGTTTCTCCGGCACCGTGCATC
TGTATTTCCAGCCCGCGGAAGAGCATGGCGTCGACAGCGGCGCAAAGAAGATGATCGACGACGGTCTGTTCGAACGCTTC
CCGTGCGACGCCGTGTTCGGCATGCACAACCATCCGGGCGCGGCGCCGGGCGTGTTTCTCACGCGGCGCGGGCCGTTCAT
GTCGGCCGGCGACAAGGCGATCATCACGATCGAAGGCGTCGGCGGCCATGCGGCGCGTCCGCATCTGACGGTCGACCCGG
TCGTCGTCGCGGCGAGCATCGTGATGGCGCTGCAGACGATCGTCGCGCGCAACGTCGATCCCGCGCAGCCGGCGGTCGTG
ACGGTCGGATCGATGCACGCGGGCACCGCGAACAACGTGATTCCGAACGGCGCGCGCCTCGAGCTGAGCGTGCGCTCGTT
CAGCCCCGACGTGCGCGCGCTGCTGAAGCGCCGCATCGTCGAACTCGCCGAAACGCAGGCCGCGAGCTACGGCGCGACGG
CGCAGGTCGAGTACATCGAGGGCTATCCGGTTGTCGTCAATACGGATGCCGAAACGGATTTCGCGGCGCAGGTCGCGCGC
GAACTCGTCGGCGACGCGCAGGTGGTCGAGCAGGCCGATCTGCTGATGGGCAGCGAGGATTTCGCGTTCATGCTGCAGCA
GCGGCCCGGTTCGTTCGTGCGGCTCGGCAACGGCGAAGGCGAGGACGGCTGCATGGTCCACAACCCGAAGTACGACTTCA
ACGACCGCAATCTGCCGATCGGCGCCGCGTTCTGGGCGCGCCTCGTCGAGCGTTATCTCGCGCAGTGA

Upstream 100 bases:

>100_bases
AAAAATTCCGCATCTTATTGAACCGATATCGGCTCGATATAGTGGCTCGCTTTCCGCATGCGCCGAGCGCATGCCGCCGA
CCTTCCGCAGGAGCCCGTTG

Downstream 100 bases:

>100_bases
CCGCACACGCGGGCGGCCCGTGCGTCGGGCCGCGTTCCAAGGAGACCTGAACGATGCAGAAAGACCCTGTCGCGCTGCAC
CCCGCGCACGCCGCGGCGAT

Product: hippurate hydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MTDTRFTEIEDLLPAAGALREIRHHIHSHPELAYEEHDTAALVADKLEQWGWQVTRGVGKTGVVGTLRVGDGTRSIGIRA
DMDALPIVEATGLPYASATHGKMHACGHDGHTTMLLGAAQHLAKTRRFSGTVHLYFQPAEEHGVDSGAKKMIDDGLFERF
PCDAVFGMHNHPGAAPGVFLTRRGPFMSAGDKAIITIEGVGGHAARPHLTVDPVVVAASIVMALQTIVARNVDPAQPAVV
TVGSMHAGTANNVIPNGARLELSVRSFSPDVRALLKRRIVELAETQAASYGATAQVEYIEGYPVVVNTDAETDFAAQVAR
ELVGDAQVVEQADLLMGSEDFAFMLQQRPGSFVRLGNGEGEDGCMVHNPKYDFNDRNLPIGAAFWARLVERYLAQ

Sequences:

>Translated_395_residues
MTDTRFTEIEDLLPAAGALREIRHHIHSHPELAYEEHDTAALVADKLEQWGWQVTRGVGKTGVVGTLRVGDGTRSIGIRA
DMDALPIVEATGLPYASATHGKMHACGHDGHTTMLLGAAQHLAKTRRFSGTVHLYFQPAEEHGVDSGAKKMIDDGLFERF
PCDAVFGMHNHPGAAPGVFLTRRGPFMSAGDKAIITIEGVGGHAARPHLTVDPVVVAASIVMALQTIVARNVDPAQPAVV
TVGSMHAGTANNVIPNGARLELSVRSFSPDVRALLKRRIVELAETQAASYGATAQVEYIEGYPVVVNTDAETDFAAQVAR
ELVGDAQVVEQADLLMGSEDFAFMLQQRPGSFVRLGNGEGEDGCMVHNPKYDFNDRNLPIGAAFWARLVERYLAQ
>Mature_394_residues
TDTRFTEIEDLLPAAGALREIRHHIHSHPELAYEEHDTAALVADKLEQWGWQVTRGVGKTGVVGTLRVGDGTRSIGIRAD
MDALPIVEATGLPYASATHGKMHACGHDGHTTMLLGAAQHLAKTRRFSGTVHLYFQPAEEHGVDSGAKKMIDDGLFERFP
CDAVFGMHNHPGAAPGVFLTRRGPFMSAGDKAIITIEGVGGHAARPHLTVDPVVVAASIVMALQTIVARNVDPAQPAVVT
VGSMHAGTANNVIPNGARLELSVRSFSPDVRALLKRRIVELAETQAASYGATAQVEYIEGYPVVVNTDAETDFAAQVARE
LVGDAQVVEQADLLMGSEDFAFMLQQRPGSFVRLGNGEGEDGCMVHNPKYDFNDRNLPIGAAFWARLVERYLAQ

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=422, Percent_Identity=28.1990521327014, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1787598, Length=196, Percent_Identity=33.1632653061224, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 42511; Mature: 42379

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTRFTEIEDLLPAAGALREIRHHIHSHPELAYEEHDTAALVADKLEQWGWQVTRGVGK
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHCCCC
TGVVGTLRVGDGTRSIGIRADMDALPIVEATGLPYASATHGKMHACGHDGHTTMLLGAAQ
CCCEEEEEECCCCCEECEEECCCCCCEEEECCCCCCCCCCCCEEECCCCCCEEHHHHHHH
HLAKTRRFSGTVHLYFQPAEEHGVDSGAKKMIDDGLFERFPCDAVFGMHNHPGAAPGVFL
HHHHHHHCCCEEEEEEECHHHCCCCHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCEEE
TRRGPFMSAGDKAIITIEGVGGHAARPHLTVDPVVVAASIVMALQTIVARNVDPAQPAVV
EECCCCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEE
TVGSMHAGTANNVIPNGARLELSVRSFSPDVRALLKRRIVELAETQAASYGATAQVEYIE
EECCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
GYPVVVNTDAETDFAAQVARELVGDAQVVEQADLLMGSEDFAFMLQQRPGSFVRLGNGEG
CCCEEEECCCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEECCCCC
EDGCMVHNPKYDFNDRNLPIGAAFWARLVERYLAQ
CCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TDTRFTEIEDLLPAAGALREIRHHIHSHPELAYEEHDTAALVADKLEQWGWQVTRGVGK
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHCCCC
TGVVGTLRVGDGTRSIGIRADMDALPIVEATGLPYASATHGKMHACGHDGHTTMLLGAAQ
CCCEEEEEECCCCCEECEEECCCCCCEEEECCCCCCCCCCCCEEECCCCCCEEHHHHHHH
HLAKTRRFSGTVHLYFQPAEEHGVDSGAKKMIDDGLFERFPCDAVFGMHNHPGAAPGVFL
HHHHHHHCCCEEEEEEECHHHCCCCHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCEEE
TRRGPFMSAGDKAIITIEGVGGHAARPHLTVDPVVVAASIVMALQTIVARNVDPAQPAVV
EECCCCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEE
TVGSMHAGTANNVIPNGARLELSVRSFSPDVRALLKRRIVELAETQAASYGATAQVEYIE
EECCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
GYPVVVNTDAETDFAAQVARELVGDAQVVEQADLLMGSEDFAFMLQQRPGSFVRLGNGEG
CCCEEEECCCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEECCCCC
EDGCMVHNPKYDFNDRNLPIGAAFWARLVERYLAQ
CCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]