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The map label for this gene is yafC [H]

Identifier: 189353921

GI number: 189353921

Start: 2235789

End: 2236736

Strand: Reverse

Name: yafC [H]

Synonym: BMULJ_05173

Alternate gene names: 189353921

Gene position: 2236736-2235789 (Counterclockwise)

Preceding gene: 189353927

Following gene: 189353916

Centisome position: 90.44

GC content: 69.41

Gene sequence:

>948_bases
ATGAAGATCACGCTCGACGAACTCCAGGCCTTTGCGGCCGTGGTCGACAGCGGTTCGATCACGGCCGCCGCGCAGCAGCT
CGATCTGACGGTGTCGGCCGCGAGCCGCACGCTCGCGCGGCTCGAGGAGAAGCTGAAGACGACGCTGCTGCGCCGGACCA
CGCGCCGGCTCGAACTGACCGAAGAAGGGCGCGCGTTCCTGCAGGATGCGCGCGCGATCATCGAATCGGTCGAAAACGCC
GAAGAGCAGATGCTCGCGCGGCGCGAGATGCCGTCGGGGCGGCTGCGCGTCGACGCGGCGACGCCGTTCATGCTGCACGT
GATCGTGCCGCTCGTGCGCGGCTATCGCGAACGCTTTCCGAAGGTCGAGCTGGAGCTGAACAGCAACGAAGGGATCATCG
ATCTGCTCGAACGGCGTACCGACGTCGCGTTCCGGATCGGCCGGCTGAAGGATTCGACGCTGCACAGCCGGCGCATCGGC
AGCAGCCGGCTGCGCATGCTCGCGAGCCCGGCGTACGTCGAGGCGCACGGCTTGCCGCGCAAGGTCGAGGATCTCGGCCG
GCACACGCTGCTCGGCTTCACGCAGCCGGAGTCGCTGAACGTCTGGCCGATCCTCGGCGCGGACGGCGAGCCGCAGCGGA
TCGAGCCCGCGATCTGGTCGTCGAGCGGCGAGACGCTGCGGCAGCTCGCGCTGGACGGCGCGGGCATCGTGTGTCTGTCG
GACTTCATGACCGCGCACGATCGCGATGCGGGCCGCCTCGTGCAGGTGCTCGCGAGGCAGACGCTCGACGTGCAGCAGCC
GATCAACGCGGTGTACTACCGCAACACGGCGATTTCGTCGCGGATCGCGTCGTTCGTCGACTATCTGGTCGACGCGCTCG
GCGGCGGCGCAGCGGCGGCCGGCAGCGCGCCGTCGCGGCGCAAGGCCGCATGGTTGAGCCCGTCGTAG

Upstream 100 bases:

>100_bases
ACGGAATCCGGCTGACGGTGAGGACGGATCGCAGTGCGCCGGCTTTACTTTTGCAGAAAAGCTCGTCTATAAACCAGATT
GCTTTGATTTCAGATCAACG

Downstream 100 bases:

>100_bases
CGGCCCGCCGGTCGCGACGCACGTCGCGCGCCGGCTCGGCCGCGTGCCGCGTCACGCTTCTTCCGACCACGACATGTTGT
CGCGCGCGAGCAGCGCCGAC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MKITLDELQAFAAVVDSGSITAAAQQLDLTVSAASRTLARLEEKLKTTLLRRTTRRLELTEEGRAFLQDARAIIESVENA
EEQMLARREMPSGRLRVDAATPFMLHVIVPLVRGYRERFPKVELELNSNEGIIDLLERRTDVAFRIGRLKDSTLHSRRIG
SSRLRMLASPAYVEAHGLPRKVEDLGRHTLLGFTQPESLNVWPILGADGEPQRIEPAIWSSSGETLRQLALDGAGIVCLS
DFMTAHDRDAGRLVQVLARQTLDVQQPINAVYYRNTAISSRIASFVDYLVDALGGGAAAAGSAPSRRKAAWLSPS

Sequences:

>Translated_315_residues
MKITLDELQAFAAVVDSGSITAAAQQLDLTVSAASRTLARLEEKLKTTLLRRTTRRLELTEEGRAFLQDARAIIESVENA
EEQMLARREMPSGRLRVDAATPFMLHVIVPLVRGYRERFPKVELELNSNEGIIDLLERRTDVAFRIGRLKDSTLHSRRIG
SSRLRMLASPAYVEAHGLPRKVEDLGRHTLLGFTQPESLNVWPILGADGEPQRIEPAIWSSSGETLRQLALDGAGIVCLS
DFMTAHDRDAGRLVQVLARQTLDVQQPINAVYYRNTAISSRIASFVDYLVDALGGGAAAAGSAPSRRKAAWLSPS
>Mature_315_residues
MKITLDELQAFAAVVDSGSITAAAQQLDLTVSAASRTLARLEEKLKTTLLRRTTRRLELTEEGRAFLQDARAIIESVENA
EEQMLARREMPSGRLRVDAATPFMLHVIVPLVRGYRERFPKVELELNSNEGIIDLLERRTDVAFRIGRLKDSTLHSRRIG
SSRLRMLASPAYVEAHGLPRKVEDLGRHTLLGFTQPESLNVWPILGADGEPQRIEPAIWSSSGETLRQLALDGAGIVCLS
DFMTAHDRDAGRLVQVLARQTLDVQQPINAVYYRNTAISSRIASFVDYLVDALGGGAAAAGSAPSRRKAAWLSPS

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=300, Percent_Identity=46, Blast_Score=279, Evalue=1e-76,
Organism=Escherichia coli, GI1789639, Length=295, Percent_Identity=30.8474576271186, Blast_Score=141, Evalue=5e-35,
Organism=Escherichia coli, GI87081978, Length=289, Percent_Identity=28.719723183391, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI1787128, Length=291, Percent_Identity=27.1477663230241, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI145693193, Length=294, Percent_Identity=27.2108843537415, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1789440, Length=285, Percent_Identity=25.6140350877193, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1787589, Length=288, Percent_Identity=26.3888888888889, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1789173, Length=293, Percent_Identity=28.6689419795222, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788481, Length=292, Percent_Identity=27.7397260273973, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87082132, Length=295, Percent_Identity=26.1016949152542, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI145693105, Length=160, Percent_Identity=27.5, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790262, Length=211, Percent_Identity=26.5402843601896, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788297, Length=140, Percent_Identity=31.4285714285714, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1788706, Length=187, Percent_Identity=25.1336898395722, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34707; Mature: 34707

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITLDELQAFAAVVDSGSITAAAQQLDLTVSAASRTLARLEEKLKTTLLRRTTRRLELT
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEGRAFLQDARAIIESVENAEEQMLARREMPSGRLRVDAATPFMLHVIVPLVRGYRERFP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCC
KVELELNSNEGIIDLLERRTDVAFRIGRLKDSTLHSRRIGSSRLRMLASPAYVEAHGLPR
EEEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHCCCCEEECCCCCH
KVEDLGRHTLLGFTQPESLNVWPILGADGEPQRIEPAIWSSSGETLRQLALDGAGIVCLS
HHHHCCCCEEEECCCCCCCCEEEEECCCCCCCCCCHHHCCCCHHHHHHHHHCCCCHHHHH
DFMTAHDRDAGRLVQVLARQTLDVQQPINAVYYRNTAISSRIASFVDYLVDALGGGAAAA
HHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCC
GSAPSRRKAAWLSPS
CCCCCCCCCCCCCCC
>Mature Secondary Structure
MKITLDELQAFAAVVDSGSITAAAQQLDLTVSAASRTLARLEEKLKTTLLRRTTRRLELT
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEGRAFLQDARAIIESVENAEEQMLARREMPSGRLRVDAATPFMLHVIVPLVRGYRERFP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCC
KVELELNSNEGIIDLLERRTDVAFRIGRLKDSTLHSRRIGSSRLRMLASPAYVEAHGLPR
EEEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHCCCCEEECCCCCH
KVEDLGRHTLLGFTQPESLNVWPILGADGEPQRIEPAIWSSSGETLRQLALDGAGIVCLS
HHHHCCCCEEEECCCCCCCCEEEEECCCCCCCCCCHHHCCCCHHHHHHHHHCCCCHHHHH
DFMTAHDRDAGRLVQVLARQTLDVQQPINAVYYRNTAISSRIASFVDYLVDALGGGAAAA
HHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCC
GSAPSRRKAAWLSPS
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]