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The map label for this gene is proP [H]

Identifier: 189353915

GI number: 189353915

Start: 2228870

End: 2230192

Strand: Reverse

Name: proP [H]

Synonym: BMULJ_05167

Alternate gene names: 189353915

Gene position: 2230192-2228870 (Counterclockwise)

Preceding gene: 189353916

Following gene: 189353913

Centisome position: 90.18

GC content: 68.48

Gene sequence:

>1323_bases
ATGCAGAAAGACCCTGTCGCGCTGCACCCCGCGCACGCCGCGGCGATGGCCGTCGGCGCGCCGGCCGGCACGCGCCGCGG
CGCGATCGCGGCGGCCGTGATCGGCAACTGGCTCGAATTCTTCGACTTCACCGTGTACGGCTTTTTCGCGGTGCTGATCG
GCAAGCTGTTCTTTCCGTCGAGCGATGCGACGACGTCGCTGCTGCTGTCGGTCGCGACGTTCGCGGCCGGCTTCTTCACG
CGGCCGCTCGGCAGCGTCGTGCTCGGCGTGTACGCGGACCGCAAGGGACGCAAGGCCGCGCTGAACCTGACGATCATGCT
GATGGCGATCGGCACCGGGCTGATCGCGATCGCGCCGACCTATGCGCAGATCGGCGTGGCTGCACCGCTGCTCGTCGTCT
GCGCGCGGCTGATGCAGGGCTTCTCGCAAGGCGGCGAATTCGGCGCGGCGACCTCGACGCTGCTCGAACAAGGCGGCGCG
TCGCGCCGCGCGTTTCGCGCGAGCTGGCAGCTCGCGACGCAGGGCGGCGCCGCGCTGATGGGTTCGGGCTTCGCGGCGCT
GCTGTCGAACACGATGACGAAGGCCGCGTTGGAAAGCTGGGGCTGGCGCCTGCCGTTTCTCGTCGGCGTGCTGATCGCGC
CGGTCGGCATGTTCTTGCGCCGCCGGCTCGCGGACGATGCGCCCGCCGACCGCCATCACTCGATCGAACGCGGCGTGCTG
CGCGAGCTGTTCTCGAAGCACGCGCGCACGGTACTGCTGCTGATGCTGACGGTGATGGGCGGCACGGTGTCGACGTACAT
CCTGACGTTCTACATGCCGACCTACGCGATCCATACGCTCGGGCTGCCGATGAAGCTGTCGATGTTCGTCGGCGTCGCGT
CGGGCTGCGTGATGCTCGTCACGTGCCCGCTGTTCGGCTGGCTGTCGGACCGCATCGGCAGCCGCCGCCTGCCGATCTTC
GTCGGCCGCGGCGTGCTCGTCGCATTGCTGTTCCCGGCGTTCTGGCTGATGAACCATCATCCGTCGCTGTCGGTGATCCT
GCCGCTGACCGCGCTGATGCTGCTGTTCTATTCGCTCGGCTCCGCATCGGAAATGGCGCTGATGTGCGAATCGCTGCCGC
GTCACGTGCGCGCGACCGGCATTTCGATCGCCTATGCGCTCGCGGTGACGCTGTTCGGCGGCACCGCGCAGCTGATCGCG
ACCTGGCTCGTGAAGGTGACGGGGAGCAAGCTGGCGCCGGCCGGCTACGTCGCAGCGTGCGTCGTGCTGTCGCTGATCGC
GGTCGCGATGCTGCGCGAGACCGCGCACGAATCGATGGACTGA

Upstream 100 bases:

>100_bases
GCGCCGCGTTCTGGGCGCGCCTCGTCGAGCGTTATCTCGCGCAGTGACCGCACACGCGGGCGGCCCGTGCGTCGGGCCGC
GTTCCAAGGAGACCTGAACG

Downstream 100 bases:

>100_bases
CGCCGCGCGCACGCGCGGCGCGCGCTCAACCGCGCCGCGCGACGAGCTCCGACACGGTCTGTGCGGCTTCCTGCAGCGGC
CCGAGAAACTCCTTGACCAT

Product: proline/betaine transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MQKDPVALHPAHAAAMAVGAPAGTRRGAIAAAVIGNWLEFFDFTVYGFFAVLIGKLFFPSSDATTSLLLSVATFAAGFFT
RPLGSVVLGVYADRKGRKAALNLTIMLMAIGTGLIAIAPTYAQIGVAAPLLVVCARLMQGFSQGGEFGAATSTLLEQGGA
SRRAFRASWQLATQGGAALMGSGFAALLSNTMTKAALESWGWRLPFLVGVLIAPVGMFLRRRLADDAPADRHHSIERGVL
RELFSKHARTVLLLMLTVMGGTVSTYILTFYMPTYAIHTLGLPMKLSMFVGVASGCVMLVTCPLFGWLSDRIGSRRLPIF
VGRGVLVALLFPAFWLMNHHPSLSVILPLTALMLLFYSLGSASEMALMCESLPRHVRATGISIAYALAVTLFGGTAQLIA
TWLVKVTGSKLAPAGYVAACVVLSLIAVAMLRETAHESMD

Sequences:

>Translated_440_residues
MQKDPVALHPAHAAAMAVGAPAGTRRGAIAAAVIGNWLEFFDFTVYGFFAVLIGKLFFPSSDATTSLLLSVATFAAGFFT
RPLGSVVLGVYADRKGRKAALNLTIMLMAIGTGLIAIAPTYAQIGVAAPLLVVCARLMQGFSQGGEFGAATSTLLEQGGA
SRRAFRASWQLATQGGAALMGSGFAALLSNTMTKAALESWGWRLPFLVGVLIAPVGMFLRRRLADDAPADRHHSIERGVL
RELFSKHARTVLLLMLTVMGGTVSTYILTFYMPTYAIHTLGLPMKLSMFVGVASGCVMLVTCPLFGWLSDRIGSRRLPIF
VGRGVLVALLFPAFWLMNHHPSLSVILPLTALMLLFYSLGSASEMALMCESLPRHVRATGISIAYALAVTLFGGTAQLIA
TWLVKVTGSKLAPAGYVAACVVLSLIAVAMLRETAHESMD
>Mature_440_residues
MQKDPVALHPAHAAAMAVGAPAGTRRGAIAAAVIGNWLEFFDFTVYGFFAVLIGKLFFPSSDATTSLLLSVATFAAGFFT
RPLGSVVLGVYADRKGRKAALNLTIMLMAIGTGLIAIAPTYAQIGVAAPLLVVCARLMQGFSQGGEFGAATSTLLEQGGA
SRRAFRASWQLATQGGAALMGSGFAALLSNTMTKAALESWGWRLPFLVGVLIAPVGMFLRRRLADDAPADRHHSIERGVL
RELFSKHARTVLLLMLTVMGGTVSTYILTFYMPTYAIHTLGLPMKLSMFVGVASGCVMLVTCPLFGWLSDRIGSRRLPIF
VGRGVLVALLFPAFWLMNHHPSLSVILPLTALMLLFYSLGSASEMALMCESLPRHVRATGISIAYALAVTLFGGTAQLIA
TWLVKVTGSKLAPAGYVAACVVLSLIAVAMLRETAHESMD

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=433, Percent_Identity=33.256351039261, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI1788942, Length=415, Percent_Identity=34.2168674698795, Blast_Score=201, Evalue=9e-53,
Organism=Escherichia coli, GI1788292, Length=419, Percent_Identity=28.8782816229117, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1789941, Length=431, Percent_Identity=34.1067285382831, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI87082404, Length=414, Percent_Identity=24.1545893719807, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI87082231, Length=210, Percent_Identity=25.7142857142857, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46688; Mature: 46688

Theoretical pI: Translated: 10.33; Mature: 10.33

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKDPVALHPAHAAAMAVGAPAGTRRGAIAAAVIGNWLEFFDFTVYGFFAVLIGKLFFPS
CCCCCCEECCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SDATTSLLLSVATFAAGFFTRPLGSVVLGVYADRKGRKAALNLTIMLMAIGTGLIAIAPT
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHCCCH
YAQIGVAAPLLVVCARLMQGFSQGGEFGAATSTLLEQGGASRRAFRASWQLATQGGAALM
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHH
GSGFAALLSNTMTKAALESWGWRLPFLVGVLIAPVGMFLRRRLADDAPADRHHSIERGVL
HCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
RELFSKHARTVLLLMLTVMGGTVSTYILTFYMPTYAIHTLGLPMKLSMFVGVASGCVMLV
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
TCPLFGWLSDRIGSRRLPIFVGRGVLVALLFPAFWLMNHHPSLSVILPLTALMLLFYSLG
HHHHHHHHHHHCCCCCCCEEECHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC
SASEMALMCESLPRHVRATGISIAYALAVTLFGGTAQLIATWLVKVTGSKLAPAGYVAAC
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHH
VVLSLIAVAMLRETAHESMD
HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQKDPVALHPAHAAAMAVGAPAGTRRGAIAAAVIGNWLEFFDFTVYGFFAVLIGKLFFPS
CCCCCCEECCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SDATTSLLLSVATFAAGFFTRPLGSVVLGVYADRKGRKAALNLTIMLMAIGTGLIAIAPT
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHCCCH
YAQIGVAAPLLVVCARLMQGFSQGGEFGAATSTLLEQGGASRRAFRASWQLATQGGAALM
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHH
GSGFAALLSNTMTKAALESWGWRLPFLVGVLIAPVGMFLRRRLADDAPADRHHSIERGVL
HCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
RELFSKHARTVLLLMLTVMGGTVSTYILTFYMPTYAIHTLGLPMKLSMFVGVASGCVMLV
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
TCPLFGWLSDRIGSRRLPIFVGRGVLVALLFPAFWLMNHHPSLSVILPLTALMLLFYSLG
HHHHHHHHHHHCCCCCCCEEECHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC
SASEMALMCESLPRHVRATGISIAYALAVTLFGGTAQLIATWLVKVTGSKLAPAGYVAAC
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHH
VVLSLIAVAMLRETAHESMD
HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]