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The map label for this gene is kefA [H]

Identifier: 189353817

GI number: 189353817

Start: 2114610

End: 2117060

Strand: Direct

Name: kefA [H]

Synonym: BMULJ_05064

Alternate gene names: 189353817

Gene position: 2114610-2117060 (Clockwise)

Preceding gene: 189353814

Following gene: 189353819

Centisome position: 85.5

GC content: 67.65

Gene sequence:

>2451_bases
ATGTTCATACAACGACTTTCTCATTTCGCCCGCCGGATTGCGCTGATCGCACTGCTGCAGTTCGCAGTCGTCGCGACCGC
GTCGGCGTTTCCGGCCGCCGCCTCGGCGCCGAGCGCGAGCGCCGTCGGCGCCGCACCCGTTCCGACGATTTCGCTTAGCG
ACGCGCTCGCGCAGCTCAAGCAGATGCAGGCCGAACAGGATCGCATCAAGCAGAAGACCTCGACGTCGACGACCAGCAAG
GAGCTCGACGCGCTCGAAGACGCGACGCAGCAGCTGAGCGCCGCGGTCGAGAAACTGCAAGGCGAGCTCACGCCGCAACG
TGTGCAGTTGCAGGCGCAGCTCGACGTGCTCGGGCCGCCGCCCGCGCCGGGAGCCGCGCCTGAAACGCCGGCCGTCGCGC
AGCAGCGCGCGACGCTGAATGCGCGCAAGGCGCAGATCGAGGCAGCGCTGAAACAGGCCGCCGATCAGAAGACCAATCTC
GCGAACCTGTCCGATCAGTTCGCGAAGCTGCATCGCAGCTTGCTCAGAAACCAGCTCGCGTTCCGCTCGGCCAGCATTTT
CAGCGCGCAGTTCTGGCTGCCGCTGTTCCATCTGCCGCCGGACGACGTACAACGGCTCGGCGATTTCCAGCGCGAACTGC
GCGACATGCTCGGCTCGTCCTGGACGCCCGGCCAGCGCGCGACCACCGTGCTGCTGCTGATCGCCGCGTTCGCGGCGTGG
CTCGGCGGACGCCGGCTGCTCGAATACGGGCTCGCGTGGATCTGCCTGAACCGTCTGCCGCCGACGCGGCTGCGCCGCAG
CGCGCTCGCGCTGTCGACGGCGGTTGCCACGCTCGTGGCGACCGCGATCGCCGTGCAGATCCTCTATCTCGCGCTCGCGC
GCCACTACACGCCGACACCGGCGATGAGCGACCTGTGGGACCAGTTCGTGAAACTCGCCGCGACCTGCGCGCTGATCGCG
GGCCTCGGTCGCGCGCTGCTCTGTACGAAGCATCCGTCGTGGCGGCTGCCCGCGCTCGCCGATCCCGTCGCGCTCGCGAT
GCGGCCGTTCCCCGGCGTGCTGGCCGCGCTGCTGCTGATATCGGGCACGCTCGAATCGATCGACCGGATCGCGGACACGA
GCCTGTCGGTCACGCTGCTCGGCCGCGGTTTCGTGTCGCTGGTCGTCGCGCTGACAGTGGGCGCGTCGCTGCTGCGCGCG
AACCGCGTGCGCAGCGCGCTGGCGGCGGCCGGCGAATCGCCCGAGCAGCGCTCGACGCTTGCCGGGCTGATCCACGCCGG
CGTGACGCTTGCGATCGTCGTGTCGCTGATCGCGCTGGCGATCGGCTACATCACGGTCGCGCGCTTCATCACGTACGAGC
TCGTGTGGTTCGAGATCGTGCTCTGCAGCGTGTACGTGCTCACGCAGCTGACGCGCGACGCGAGCGAGAGCCTGTTCTCG
GTGAACCTGTCGACGGGCAAGCAGATCAAGCATCTGTTCGCGCTCGACGACCGGCATCTCGAACAGGCACACACCGTCGT
GTCGGGCATCGGCACGAGCCTGCTGATGCTCGTCGCGGCGATCGCGCTGCTGACGGGCGGCTTCGGCACAACGCCGAGCG
ATCTGCTCGACAGCGCGATCGCGATGATCGGCGGCCAGCGGCTGCAGAGCCTGAACATCATGCCGGACCGGATCATGAAC
GCGGTGATCGGGTTCGCGATCGGCTTCTATCTGCTGCGCTCGGTACGGCGCTGGCTCGACAACGAGTTCCTGCCGGCGCT
CGGCATGGACAGCGGCATGCGCGCGTCGCTCGTCACGCTGTTTGCGAACGTCGGCTATGTGCTGCTCGTGCTGATGACGC
TCGGGCTGCTCGGCGTGAAGTGGAGCAACCTCGCGTGGATCGTCAGCGCGCTGTCGGTCGGTATCGGCTTCGGCCTGCAG
GAGATCGTGAAGAACTTCGTGTCGGGGCTGATCCTGCTGACGGAGCGGCCGGTGAAGGTCGGCGACATGATCAGCATCGC
GGGCGTCGAAGGCGACATCCGCCGCATCAACGTGCGCGCAACCGAGATTCAGCTCAGCGATCGCTCGACCGTGATCGTGC
CGAATTCGCAGCTGATCTCGCAGAACCTGCGCAACGTGACGATGGGCAACAGCACGCAGGGCGTCGCGACGCTGATGCTG
ACGTTCCCGCTGAACACCGATCCGGAACAGGTGCGCGACCTGCTGCTCGAGGCGTACCGCGCCCACCCGGCGATTCTCGA
GAAGCCGGCGCCGTCGGTCACGTTCAGCCAGCTCGCGCCGGACGGCATTACGCTGAGCGTGACGGGCTACGTGGCGAGCC
CGCGCATCGCGGGTTCGACGAAGAGCGACTTGCTGTTCGAGATCCTGAAGCAGCTGCGTGCGGCCGGCATCACGCTGTCG
AGCCCGCAGATGCTGGTCGTGCAGAACATGCCGTCGGCCGACGGCGCCTGA

Upstream 100 bases:

>100_bases
TCGCCGCCGCCGTCGCGCCCCGGTAACATGCTACCGGGCCAACCGCCGGCCGCCCGATGCCTGTCGGCAAACGGACATGT
CGGCAAATCGTAAGCAATCC

Downstream 100 bases:

>100_bases
GCGCGGCGCGCGCCGCCACCGCGCATCGCTTCCCCGCAACGAAGAAGGCCGGTCGCCGCATCGCGGCAACCGGCCTTTGG
CATTGACGCGCAGCGCCCTT

Product: mechanosensitive ion channel

Products: NA

Alternate protein names: Protein AefA [H]

Number of amino acids: Translated: 816; Mature: 816

Protein sequence:

>816_residues
MFIQRLSHFARRIALIALLQFAVVATASAFPAAASAPSASAVGAAPVPTISLSDALAQLKQMQAEQDRIKQKTSTSTTSK
ELDALEDATQQLSAAVEKLQGELTPQRVQLQAQLDVLGPPPAPGAAPETPAVAQQRATLNARKAQIEAALKQAADQKTNL
ANLSDQFAKLHRSLLRNQLAFRSASIFSAQFWLPLFHLPPDDVQRLGDFQRELRDMLGSSWTPGQRATTVLLLIAAFAAW
LGGRRLLEYGLAWICLNRLPPTRLRRSALALSTAVATLVATAIAVQILYLALARHYTPTPAMSDLWDQFVKLAATCALIA
GLGRALLCTKHPSWRLPALADPVALAMRPFPGVLAALLLISGTLESIDRIADTSLSVTLLGRGFVSLVVALTVGASLLRA
NRVRSALAAAGESPEQRSTLAGLIHAGVTLAIVVSLIALAIGYITVARFITYELVWFEIVLCSVYVLTQLTRDASESLFS
VNLSTGKQIKHLFALDDRHLEQAHTVVSGIGTSLLMLVAAIALLTGGFGTTPSDLLDSAIAMIGGQRLQSLNIMPDRIMN
AVIGFAIGFYLLRSVRRWLDNEFLPALGMDSGMRASLVTLFANVGYVLLVLMTLGLLGVKWSNLAWIVSALSVGIGFGLQ
EIVKNFVSGLILLTERPVKVGDMISIAGVEGDIRRINVRATEIQLSDRSTVIVPNSQLISQNLRNVTMGNSTQGVATLML
TFPLNTDPEQVRDLLLEAYRAHPAILEKPAPSVTFSQLAPDGITLSVTGYVASPRIAGSTKSDLLFEILKQLRAAGITLS
SPQMLVVQNMPSADGA

Sequences:

>Translated_816_residues
MFIQRLSHFARRIALIALLQFAVVATASAFPAAASAPSASAVGAAPVPTISLSDALAQLKQMQAEQDRIKQKTSTSTTSK
ELDALEDATQQLSAAVEKLQGELTPQRVQLQAQLDVLGPPPAPGAAPETPAVAQQRATLNARKAQIEAALKQAADQKTNL
ANLSDQFAKLHRSLLRNQLAFRSASIFSAQFWLPLFHLPPDDVQRLGDFQRELRDMLGSSWTPGQRATTVLLLIAAFAAW
LGGRRLLEYGLAWICLNRLPPTRLRRSALALSTAVATLVATAIAVQILYLALARHYTPTPAMSDLWDQFVKLAATCALIA
GLGRALLCTKHPSWRLPALADPVALAMRPFPGVLAALLLISGTLESIDRIADTSLSVTLLGRGFVSLVVALTVGASLLRA
NRVRSALAAAGESPEQRSTLAGLIHAGVTLAIVVSLIALAIGYITVARFITYELVWFEIVLCSVYVLTQLTRDASESLFS
VNLSTGKQIKHLFALDDRHLEQAHTVVSGIGTSLLMLVAAIALLTGGFGTTPSDLLDSAIAMIGGQRLQSLNIMPDRIMN
AVIGFAIGFYLLRSVRRWLDNEFLPALGMDSGMRASLVTLFANVGYVLLVLMTLGLLGVKWSNLAWIVSALSVGIGFGLQ
EIVKNFVSGLILLTERPVKVGDMISIAGVEGDIRRINVRATEIQLSDRSTVIVPNSQLISQNLRNVTMGNSTQGVATLML
TFPLNTDPEQVRDLLLEAYRAHPAILEKPAPSVTFSQLAPDGITLSVTGYVASPRIAGSTKSDLLFEILKQLRAAGITLS
SPQMLVVQNMPSADGA
>Mature_816_residues
MFIQRLSHFARRIALIALLQFAVVATASAFPAAASAPSASAVGAAPVPTISLSDALAQLKQMQAEQDRIKQKTSTSTTSK
ELDALEDATQQLSAAVEKLQGELTPQRVQLQAQLDVLGPPPAPGAAPETPAVAQQRATLNARKAQIEAALKQAADQKTNL
ANLSDQFAKLHRSLLRNQLAFRSASIFSAQFWLPLFHLPPDDVQRLGDFQRELRDMLGSSWTPGQRATTVLLLIAAFAAW
LGGRRLLEYGLAWICLNRLPPTRLRRSALALSTAVATLVATAIAVQILYLALARHYTPTPAMSDLWDQFVKLAATCALIA
GLGRALLCTKHPSWRLPALADPVALAMRPFPGVLAALLLISGTLESIDRIADTSLSVTLLGRGFVSLVVALTVGASLLRA
NRVRSALAAAGESPEQRSTLAGLIHAGVTLAIVVSLIALAIGYITVARFITYELVWFEIVLCSVYVLTQLTRDASESLFS
VNLSTGKQIKHLFALDDRHLEQAHTVVSGIGTSLLMLVAAIALLTGGFGTTPSDLLDSAIAMIGGQRLQSLNIMPDRIMN
AVIGFAIGFYLLRSVRRWLDNEFLPALGMDSGMRASLVTLFANVGYVLLVLMTLGLLGVKWSNLAWIVSALSVGIGFGLQ
EIVKNFVSGLILLTERPVKVGDMISIAGVEGDIRRINVRATEIQLSDRSTVIVPNSQLISQNLRNVTMGNSTQGVATLML
TFPLNTDPEQVRDLLLEAYRAHPAILEKPAPSVTFSQLAPDGITLSVTGYVASPRIAGSTKSDLLFEILKQLRAAGITLS
SPQMLVVQNMPSADGA

Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=255, Percent_Identity=32.9411764705882, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI2367355, Length=409, Percent_Identity=27.3838630806846, Blast_Score=132, Evalue=1e-31,
Organism=Escherichia coli, GI1789291, Length=191, Percent_Identity=23.5602094240838, Blast_Score=74, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 87368; Mature: 87368

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFIQRLSHFARRIALIALLQFAVVATASAFPAAASAPSASAVGAAPVPTISLSDALAQLK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
QMQAEQDRIKQKTSTSTTSKELDALEDATQQLSAAVEKLQGELTPQRVQLQAQLDVLGPP
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEEEHEECCCCC
PAPGAAPETPAVAQQRATLNARKAQIEAALKQAADQKTNLANLSDQFAKLHRSLLRNQLA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
FRSASIFSAQFWLPLFHLPPDDVQRLGDFQRELRDMLGSSWTPGQRATTVLLLIAAFAAW
HHHHHHHHHHHHCCEECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
LGGRRLLEYGLAWICLNRLPPTRLRRSALALSTAVATLVATAIAVQILYLALARHYTPTP
HCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
AMSDLWDQFVKLAATCALIAGLGRALLCTKHPSWRLPALADPVALAMRPFPGVLAALLLI
CHHHHHHHHHHHHHHHHHHHHCCHHEEECCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHH
SGTLESIDRIADTSLSVTLLGRGFVSLVVALTVGASLLRANRVRSALAAAGESPEQRSTL
HHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
AGLIHAGVTLAIVVSLIALAIGYITVARFITYELVWFEIVLCSVYVLTQLTRDASESLFS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
VNLSTGKQIKHLFALDDRHLEQAHTVVSGIGTSLLMLVAAIALLTGGFGTTPSDLLDSAI
EECCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
AMIGGQRLQSLNIMPDRIMNAVIGFAIGFYLLRSVRRWLDNEFLPALGMDSGMRASLVTL
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
FANVGYVLLVLMTLGLLGVKWSNLAWIVSALSVGIGFGLQEIVKNFVSGLILLTERPVKV
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEEEECCCCCC
GDMISIAGVEGDIRRINVRATEIQLSDRSTVIVPNSQLISQNLRNVTMGNSTQGVATLML
CCEEEEECCCCCCEEEEEEEEEEEECCCCEEEECCHHHHHHHHHHCCCCCCCCCCEEEEE
TFPLNTDPEQVRDLLLEAYRAHPAILEKPAPSVTFSQLAPDGITLSVTGYVASPRIAGST
EECCCCCHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHCCCCCEEEEEEEEEECCCCCCCC
KSDLLFEILKQLRAAGITLSSPQMLVVQNMPSADGA
HHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCC
>Mature Secondary Structure
MFIQRLSHFARRIALIALLQFAVVATASAFPAAASAPSASAVGAAPVPTISLSDALAQLK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
QMQAEQDRIKQKTSTSTTSKELDALEDATQQLSAAVEKLQGELTPQRVQLQAQLDVLGPP
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEEEHEECCCCC
PAPGAAPETPAVAQQRATLNARKAQIEAALKQAADQKTNLANLSDQFAKLHRSLLRNQLA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
FRSASIFSAQFWLPLFHLPPDDVQRLGDFQRELRDMLGSSWTPGQRATTVLLLIAAFAAW
HHHHHHHHHHHHCCEECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
LGGRRLLEYGLAWICLNRLPPTRLRRSALALSTAVATLVATAIAVQILYLALARHYTPTP
HCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
AMSDLWDQFVKLAATCALIAGLGRALLCTKHPSWRLPALADPVALAMRPFPGVLAALLLI
CHHHHHHHHHHHHHHHHHHHHCCHHEEECCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHH
SGTLESIDRIADTSLSVTLLGRGFVSLVVALTVGASLLRANRVRSALAAAGESPEQRSTL
HHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
AGLIHAGVTLAIVVSLIALAIGYITVARFITYELVWFEIVLCSVYVLTQLTRDASESLFS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
VNLSTGKQIKHLFALDDRHLEQAHTVVSGIGTSLLMLVAAIALLTGGFGTTPSDLLDSAI
EECCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
AMIGGQRLQSLNIMPDRIMNAVIGFAIGFYLLRSVRRWLDNEFLPALGMDSGMRASLVTL
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
FANVGYVLLVLMTLGLLGVKWSNLAWIVSALSVGIGFGLQEIVKNFVSGLILLTERPVKV
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEEEECCCCCC
GDMISIAGVEGDIRRINVRATEIQLSDRSTVIVPNSQLISQNLRNVTMGNSTQGVATLML
CCEEEEECCCCCCEEEEEEEEEEEECCCCEEEECCHHHHHHHHHHCCCCCCCCCCEEEEE
TFPLNTDPEQVRDLLLEAYRAHPAILEKPAPSVTFSQLAPDGITLSVTGYVASPRIAGST
EECCCCCHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHCCCCCEEEEEEEEEECCCCCCCC
KSDLLFEILKQLRAAGITLSSPQMLVVQNMPSADGA
HHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 10202137 [H]