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The map label for this gene is emrB [H]

Identifier: 189352319

GI number: 189352319

Start: 369489

End: 371063

Strand: Reverse

Name: emrB [H]

Synonym: BMULJ_03538

Alternate gene names: 189352319

Gene position: 371063-369489 (Counterclockwise)

Preceding gene: 189352320

Following gene: 189352318

Centisome position: 15.0

GC content: 65.27

Gene sequence:

>1575_bases
ATGACCACCGCGTTTTCCGGCGACCCGGCGTCGCAACCGACGAAGCAGCGCGTGTTCGCATTCGCGCTGATGTGTGTCGG
CTTTTTCATGGCGACGCTCGACATCCAGATCGTCGCGTCGTCGCTGCGCGACATCGGCGGCGGACTCTCGGCAAGCCAGG
ACGAACTGTCGTGGGTGCAGACGGCCTATCTGATCGCCGAGATCATCGTGATCCCGATGTCGGGCTGGCTGTCGAAGGTA
ATGTCGACACGCTGGCTGTTCGTCGCATCGGCCGTCGGCTTCACGATCACGAGCATGCTGTGCGGGCTTGCGTGGGACAT
CAATTCGATGATCCTGTTTCGCGGACTGCAGGGCGCACTCGGCGCCGCAATGATTCCGACCGTGTTCACGACCGCGTTCG
TGCTGTTTCCCGGCAGGCAGCGGCTGATCGCGTCGACGACGATCGGCGCGCTCGCGTCGCTCGCACCGGCGATCGGCCCG
GTGATCGGCGGCTGGATCACCGATCGATGGTCGTGGCACTGGCTGTTCTATCTGAATCTCGTGCCGGGCATCCTGGTCGC
GGTGCTGGTGCCGCGCTACGTGCGCATCGACGAGCCCGATCCGGGACTCATCAAGCGCGGCGACTATCTCGGGATCGCGC
TGATGTCGGGTTTTCTCGGCTGCCTCGAATACGTGCTCGAGGAAGGGCCGCGCAAGAACTGGTTCGGCGATCACGTCATC
GTTGCATGCGCATGGATCGCCGGCATCTGCGGCTTCCTGTTCGTCGTGCACGCGCTGACCGCCGAGGATCCGGTCGTCGA
TCTGCGTGCGCTTGCCGTGCGCAACTTCGGCATCGGCAGCCTGCTCTCGTTCGTCACCGGCATCGGCATCTTCGTGACCG
TATTCCTGACGCCGCTGTTTCTCGCCCAGGTGCGCGCATTCAGCTCGCTGCAGATCGGCATCGCGCTGCTGTCGGTCGGC
GCGTTCCAGTTGCTCGCGCTCTGTGCATACGCGTTCGCGGCCCGCTACTTCAGCCTGCGCTCGCTGCTCGTGTTCGGGCT
CGTGTGTTTCGGCGTCGGCTGCTATCTGTACACGCCGATCACGCACGACTGGGGCTGGCAGGAACTGCTGCTGCCGCAGG
CGCTACGCGGCATCGGTCAGCAGTTCAGCGTGCCGCCGATCGTGACGATGGCGCTCGGTTCGCTGCCGCCGTCGCGGCTG
AAATCCGCCAGCGGGCTGTTCAACCTGATGCGCAATCTCGGCGGTGCGATCGGCATCGCGGTCAGCGCGACGATGCTCAA
CGACCGGCTGAACTTCCACTACCTGCGGCTCAACGAGCACATGAGCGCCGGCGTGCCGCAGGTCGCGTCGCTGCTCGACG
GCCGCACCGCGTACTGGGCGTCGGTCGCCGGCAACACGGTCGATGCCGCGCAGGCTGCGCTCGCGAATCTGCATCGGCTG
ATCTACCGCGAAGCGCTGACGCTCACGTATGCCGATGCGTACTACGCGCTGTCGCTCTGTTTCGTCGTCGCGCTGGTCGC
GGTCGTCTTCTCTCAACCCATCTCGCTGAACGCGCCGCCGCCGGATGCGCACTGA

Upstream 100 bases:

>100_bases
CATCTCGATCCGCAGCCGGGACTCGAACAGGTGCTGCGTCCGGGGCTGTCCGCGGTCGTGACCGTGCACACCGACCACGC
GCACTGAACGGGAGACGACG

Downstream 100 bases:

>100_bases
GGTCGTCATCATGAAACAGCTCATTGTCGCGCTCGCCGTCAGCGCGTTCGCCGCCGGTTGCGCGGTACAGCCGGCGCAGC
ATGCGACGCTGCCCGACGCG

Product: permease of the major facilitator superfamily

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 524; Mature: 523

Protein sequence:

>524_residues
MTTAFSGDPASQPTKQRVFAFALMCVGFFMATLDIQIVASSLRDIGGGLSASQDELSWVQTAYLIAEIIVIPMSGWLSKV
MSTRWLFVASAVGFTITSMLCGLAWDINSMILFRGLQGALGAAMIPTVFTTAFVLFPGRQRLIASTTIGALASLAPAIGP
VIGGWITDRWSWHWLFYLNLVPGILVAVLVPRYVRIDEPDPGLIKRGDYLGIALMSGFLGCLEYVLEEGPRKNWFGDHVI
VACAWIAGICGFLFVVHALTAEDPVVDLRALAVRNFGIGSLLSFVTGIGIFVTVFLTPLFLAQVRAFSSLQIGIALLSVG
AFQLLALCAYAFAARYFSLRSLLVFGLVCFGVGCYLYTPITHDWGWQELLLPQALRGIGQQFSVPPIVTMALGSLPPSRL
KSASGLFNLMRNLGGAIGIAVSATMLNDRLNFHYLRLNEHMSAGVPQVASLLDGRTAYWASVAGNTVDAAQAALANLHRL
IYREALTLTYADAYYALSLCFVVALVAVVFSQPISLNAPPPDAH

Sequences:

>Translated_524_residues
MTTAFSGDPASQPTKQRVFAFALMCVGFFMATLDIQIVASSLRDIGGGLSASQDELSWVQTAYLIAEIIVIPMSGWLSKV
MSTRWLFVASAVGFTITSMLCGLAWDINSMILFRGLQGALGAAMIPTVFTTAFVLFPGRQRLIASTTIGALASLAPAIGP
VIGGWITDRWSWHWLFYLNLVPGILVAVLVPRYVRIDEPDPGLIKRGDYLGIALMSGFLGCLEYVLEEGPRKNWFGDHVI
VACAWIAGICGFLFVVHALTAEDPVVDLRALAVRNFGIGSLLSFVTGIGIFVTVFLTPLFLAQVRAFSSLQIGIALLSVG
AFQLLALCAYAFAARYFSLRSLLVFGLVCFGVGCYLYTPITHDWGWQELLLPQALRGIGQQFSVPPIVTMALGSLPPSRL
KSASGLFNLMRNLGGAIGIAVSATMLNDRLNFHYLRLNEHMSAGVPQVASLLDGRTAYWASVAGNTVDAAQAALANLHRL
IYREALTLTYADAYYALSLCFVVALVAVVFSQPISLNAPPPDAH
>Mature_523_residues
TTAFSGDPASQPTKQRVFAFALMCVGFFMATLDIQIVASSLRDIGGGLSASQDELSWVQTAYLIAEIIVIPMSGWLSKVM
STRWLFVASAVGFTITSMLCGLAWDINSMILFRGLQGALGAAMIPTVFTTAFVLFPGRQRLIASTTIGALASLAPAIGPV
IGGWITDRWSWHWLFYLNLVPGILVAVLVPRYVRIDEPDPGLIKRGDYLGIALMSGFLGCLEYVLEEGPRKNWFGDHVIV
ACAWIAGICGFLFVVHALTAEDPVVDLRALAVRNFGIGSLLSFVTGIGIFVTVFLTPLFLAQVRAFSSLQIGIALLSVGA
FQLLALCAYAFAARYFSLRSLLVFGLVCFGVGCYLYTPITHDWGWQELLLPQALRGIGQQFSVPPIVTMALGSLPPSRLK
SASGLFNLMRNLGGAIGIAVSATMLNDRLNFHYLRLNEHMSAGVPQVASLLDGRTAYWASVAGNTVDAAQAALANLHRLI
YREALTLTYADAYYALSLCFVVALVAVVFSQPISLNAPPPDAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=487, Percent_Identity=28.3367556468172, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1788710, Length=489, Percent_Identity=25.9713701431493, Blast_Score=172, Evalue=6e-44,
Organism=Escherichia coli, GI1788392, Length=414, Percent_Identity=26.0869565217391, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1790195, Length=415, Percent_Identity=25.7831325301205, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI87081983, Length=428, Percent_Identity=23.8317757009346, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 56504; Mature: 56373

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TTAFSGDPASQPTKQRVFAFALMCVGFFMATLDIQIVASSLRDIGGGLSASQDELSWVQ
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]