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The map label for this gene is oprM [H]

Identifier: 189352318

GI number: 189352318

Start: 368048

End: 369478

Strand: Reverse

Name: oprM [H]

Synonym: BMULJ_03537

Alternate gene names: 189352318

Gene position: 369478-368048 (Counterclockwise)

Preceding gene: 189352319

Following gene: 189352315

Centisome position: 14.94

GC content: 71.42

Gene sequence:

>1431_bases
ATGAAACAGCTCATTGTCGCGCTCGCCGTCAGCGCGTTCGCCGCCGGTTGCGCGGTACAGCCGGCGCAGCATGCGACGCT
GCCCGACGCGGTCGGCGCGCTCGCGCCTGCTACATGGGACACCGCCGTTCCGGCCGCCGCGAGCGACGCGGCGACGTGGT
GGGCGCAGTTCCGCGACCCGACGCTCGACCGACTGATCGCGACCGTGCTTGACGGCAACCTCGATCTGCAGGCCGCGGCC
GAACGCATAAAGCAGGCGCAGGCGCTGACCGCGAGCAAGCGCGCGGCGCTGCTGCCCGAACTCGACGCCACGGCGCATGC
GTCCGACGCGCGCCAGAACGTTCCGCCGCCGCTCGGCTACGTGCGGCAGGCCGGCGCGGGGCTCGCGCTGAGCTGGTCGC
CCGACGTGTTCGGCGGCGAACGGCTCGACCTGCTCGCCGCGCGTGCGGAACTCGTCGGGCAGACGCACGCGGAAGATGCG
ATGCGGCTTGCGCTGGCGGCCGACGCGGCATCCGCGTACGTCGATCTGCGCTGGGCGCAGCAGGAACTGAAGCTGCTCCA
GGACAACGCGGCGATCCGGCAGCGTGCGCTCGAACTGACACGCAAGCGGCAGGCGTTCGGGCTGTCGACGGAGCTCGACG
TCACGCGCGCGAAGAATCAGCTCGATGCGCTCGAAGCGCGGATCGCGCCGACGCGCGCGCAGATCGCGCATCAGCTGAAC
CTGATCGCCGTGTATTCGGGCCGCACGCCGGAATCGATCGACCAGCTCGTGCTCGCCGAGTCCGCGCCGATTCCCGCGCC
GCCGGCCGGCGCACCGATCACGCTGCCGTCGCAGGCGCTGCTGCGCCGCCCCGACGTGCTGACCGCCTACGCGCAGGTCG
AACGGCGGGCGGCGCAAGTCGGCGTCGCGAATGCCGAGCGGTATCCGAAGTTCTCGCTGAATCTGGCCGACGGCGTGCTG
GCCGCGTCCTATCTCGGCCTGCCGACGCTCACCGACAATCTGTTCAGCGCCGCGCTGAGCGCGACGAGCCCGATCTTCGA
TGCCGGCCGTATCGCGGCCGACATCGCGCAGAGCGAAAGCCGCTTGCGCGAATCGGAACTCGCGCTGCGGCAGACGATGC
TGCAGGCCTTGCGCGAAGTCGAGGATGCGCGCGCGAGCCTCGTCGCGACCGACGCGGCGTCGCAGCAGCTCGGCAGCGCG
CTCGCCGCGTCCGACCGCGCGCTCGCGCTCGCCACGCGACTGTACAAAGGCGGCGCGACGGACTTTCTCGATGTGCTGAC
CGCGCAGGAGGCGTATCTGCGCGACTCCGATTCGCTGAATCAGGCCGCGCGCGAGCATGCGCTCGCGGCGATCGCGCTGT
ATCGATCGCTCGGCGGCGGATGGAGCAGGAACGATGCGGGGCAGGCAGCCGCGGCAGTCGCGAGCCGGTGA

Upstream 100 bases:

>100_bases
GCGCTGTCGCTCTGTTTCGTCGTCGCGCTGGTCGCGGTCGTCTTCTCTCAACCCATCTCGCTGAACGCGCCGCCGCCGGA
TGCGCACTGAGGTCGTCATC

Downstream 100 bases:

>100_bases
GCGCGAACGCGATGCGCTGCGGTGTGGTCGGAGAACGTCGCCGAAGCGGCGAGGAGGCAATCATGGCAGTCATGGCCGCA
TGCGGCGGCCATGACTGCCG

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MKQLIVALAVSAFAAGCAVQPAQHATLPDAVGALAPATWDTAVPAAASDAATWWAQFRDPTLDRLIATVLDGNLDLQAAA
ERIKQAQALTASKRAALLPELDATAHASDARQNVPPPLGYVRQAGAGLALSWSPDVFGGERLDLLAARAELVGQTHAEDA
MRLALAADAASAYVDLRWAQQELKLLQDNAAIRQRALELTRKRQAFGLSTELDVTRAKNQLDALEARIAPTRAQIAHQLN
LIAVYSGRTPESIDQLVLAESAPIPAPPAGAPITLPSQALLRRPDVLTAYAQVERRAAQVGVANAERYPKFSLNLADGVL
AASYLGLPTLTDNLFSAALSATSPIFDAGRIAADIAQSESRLRESELALRQTMLQALREVEDARASLVATDAASQQLGSA
LAASDRALALATRLYKGGATDFLDVLTAQEAYLRDSDSLNQAAREHALAAIALYRSLGGGWSRNDAGQAAAAVASR

Sequences:

>Translated_476_residues
MKQLIVALAVSAFAAGCAVQPAQHATLPDAVGALAPATWDTAVPAAASDAATWWAQFRDPTLDRLIATVLDGNLDLQAAA
ERIKQAQALTASKRAALLPELDATAHASDARQNVPPPLGYVRQAGAGLALSWSPDVFGGERLDLLAARAELVGQTHAEDA
MRLALAADAASAYVDLRWAQQELKLLQDNAAIRQRALELTRKRQAFGLSTELDVTRAKNQLDALEARIAPTRAQIAHQLN
LIAVYSGRTPESIDQLVLAESAPIPAPPAGAPITLPSQALLRRPDVLTAYAQVERRAAQVGVANAERYPKFSLNLADGVL
AASYLGLPTLTDNLFSAALSATSPIFDAGRIAADIAQSESRLRESELALRQTMLQALREVEDARASLVATDAASQQLGSA
LAASDRALALATRLYKGGATDFLDVLTAQEAYLRDSDSLNQAAREHALAAIALYRSLGGGWSRNDAGQAAAAVASR
>Mature_476_residues
MKQLIVALAVSAFAAGCAVQPAQHATLPDAVGALAPATWDTAVPAAASDAATWWAQFRDPTLDRLIATVLDGNLDLQAAA
ERIKQAQALTASKRAALLPELDATAHASDARQNVPPPLGYVRQAGAGLALSWSPDVFGGERLDLLAARAELVGQTHAEDA
MRLALAADAASAYVDLRWAQQELKLLQDNAAIRQRALELTRKRQAFGLSTELDVTRAKNQLDALEARIAPTRAQIAHQLN
LIAVYSGRTPESIDQLVLAESAPIPAPPAGAPITLPSQALLRRPDVLTAYAQVERRAAQVGVANAERYPKFSLNLADGVL
AASYLGLPTLTDNLFSAALSATSPIFDAGRIAADIAQSESRLRESELALRQTMLQALREVEDARASLVATDAASQQLGSA
LAASDRALALATRLYKGGATDFLDVLTAQEAYLRDSDSLNQAAREHALAAIALYRSLGGGWSRNDAGQAAAAVASR

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=466, Percent_Identity=26.8240343347639, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI2367348, Length=488, Percent_Identity=25.6147540983607, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 50157; Mature: 50157

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQLIVALAVSAFAAGCAVQPAQHATLPDAVGALAPATWDTAVPAAASDAATWWAQFRDP
CHHHHHHHHHHHHHHCCCCCCHHCCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCC
TLDRLIATVLDGNLDLQAAAERIKQAQALTASKRAALLPELDATAHASDARQNVPPPLGY
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCHHH
VRQAGAGLALSWSPDVFGGERLDLLAARAELVGQTHAEDAMRLALAADAASAYVDLRWAQ
HHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QELKLLQDNAAIRQRALELTRKRQAFGLSTELDVTRAKNQLDALEARIAPTRAQIAHQLN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCC
LIAVYSGRTPESIDQLVLAESAPIPAPPAGAPITLPSQALLRRPDVLTAYAQVERRAAQV
EEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHH
GVANAERYPKFSLNLADGVLAASYLGLPTLTDNLFSAALSATSPIFDAGRIAADIAQSES
CCCCHHHCCCEECCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
RLRESELALRQTMLQALREVEDARASLVATDAASQQLGSALAASDRALALATRLYKGGAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCH
DFLDVLTAQEAYLRDSDSLNQAAREHALAAIALYRSLGGGWSRNDAGQAAAAVASR
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKQLIVALAVSAFAAGCAVQPAQHATLPDAVGALAPATWDTAVPAAASDAATWWAQFRDP
CHHHHHHHHHHHHHHCCCCCCHHCCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCC
TLDRLIATVLDGNLDLQAAAERIKQAQALTASKRAALLPELDATAHASDARQNVPPPLGY
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCHHH
VRQAGAGLALSWSPDVFGGERLDLLAARAELVGQTHAEDAMRLALAADAASAYVDLRWAQ
HHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QELKLLQDNAAIRQRALELTRKRQAFGLSTELDVTRAKNQLDALEARIAPTRAQIAHQLN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCC
LIAVYSGRTPESIDQLVLAESAPIPAPPAGAPITLPSQALLRRPDVLTAYAQVERRAAQV
EEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHH
GVANAERYPKFSLNLADGVLAASYLGLPTLTDNLFSAALSATSPIFDAGRIAADIAQSES
CCCCHHHCCCEECCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
RLRESELALRQTMLQALREVEDARASLVATDAASQQLGSALAASDRALALATRLYKGGAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCH
DFLDVLTAQEAYLRDSDSLNQAAREHALAAIALYRSLGGGWSRNDAGQAAAAVASR
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]