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The map label for this gene is emrA [H]

Identifier: 189352320

GI number: 189352320

Start: 371077

End: 372168

Strand: Reverse

Name: emrA [H]

Synonym: BMULJ_03539

Alternate gene names: 189352320

Gene position: 372168-371077 (Counterclockwise)

Preceding gene: 189352321

Following gene: 189352319

Centisome position: 15.05

GC content: 70.79

Gene sequence:

>1092_bases
ATGGACACGACTCAAACCGCAGCCGCCCGCGAAGCGGCCACGGCCGAACCGCCCGCGAAGCAGCGCCGCCGCGTACGCTG
GATGCGCATCGCCGTGATCGCCGTCGTCGTCGCGGTCGGCGCGGCCGCGCTCGACTGGCTGTTCGTGCTGCGTTTCCAGG
AAAGCACCGACGACGCGTATGTCGGCGGCGACGTGACGGTGCTCGCGCCGAAGGTCGCCGGCTTCGTCGACCGGATCGGG
GTGACCGACAACCAGCGCGTGAAGGCCGGCGACGTCCTCGTACAGCTCGACGCGCGCGACTACGCGGCGAAGCTCGCGCA
GGCGAGCGCCGAAGTCGACGCGGCGCGCTCGGCCGTGGCCGAGCTCGAAGCGAAGCAGCAGCTGCAAGACGCGGTGATCG
GCCAGCATGCGGCCGACAAGAACGCATCGTCGGCCGAGCTGACGCGCGCCGCCGCCGATCGCGTCCGCTATCGCGAACTC
GTGAAGGCCGACGCGGTGTCGAATCAGCTCGTCGAGCGCGCCGATGCCGACTACGCGAAGGCGCACGCGGCCGTCGAACG
CAGCGATGCGGCGCTGCTCGCGTCGAAGCGTGAGCTTGACGTGCTCGGCGCGCAGCTCGCCGATGCACGCGCCCGCGTGA
ACACCGCCCTCGCCGCGCAGCGCGTTGCCGCATTGAACGTCGAATACACGACGATCCGTGCACCGGTCGACGGCTACGTC
GGCAACCGCACCGGCCGCGTCGGGATGCTCGCGAACGTCGGCGTGCCGCTGCTGACGATCGTCCCTGCGTCCGGCTTGTG
GATCGACGCGAACTTCAAGGAGGACCAGCTGCGCAAGATGCGCGCGGGCGATCGCGTCGACGTGTCGCTCGATGCGTCGG
CCGCGCGGCTGCACGGCCGCGTCGACAGCGTCGCGCCCGCGACCGGCGCGACGTTCAGCGTGCTGCCGCCCGAGAATGCA
ACCGGCAACTTCACGAAGATCGTGCAGCGCGTGCCGGTGCGCGTCCATCTCGATCCGCAGCCGGGACTCGAACAGGTGCT
GCGTCCGGGGCTGTCCGCGGTCGTGACCGTGCACACCGACCACGCGCACTGA

Upstream 100 bases:

>100_bases
CGGCGCGCTGACGGACTTCCTATCCGTCGCGCGCAGGCGTTTTCCCGATCGACGCATCGCCGTCGGTCGACTTGATGAGT
GCATATACATAGGTGAATCG

Downstream 100 bases:

>100_bases
ACGGGAGACGACGATGACCACCGCGTTTTCCGGCGACCCGGCGTCGCAACCGACGAAGCAGCGCGTGTTCGCATTCGCGC
TGATGTGTGTCGGCTTTTTC

Product: putative multidrug resistance efflux pump

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MDTTQTAAAREAATAEPPAKQRRRVRWMRIAVIAVVVAVGAAALDWLFVLRFQESTDDAYVGGDVTVLAPKVAGFVDRIG
VTDNQRVKAGDVLVQLDARDYAAKLAQASAEVDAARSAVAELEAKQQLQDAVIGQHAADKNASSAELTRAAADRVRYREL
VKADAVSNQLVERADADYAKAHAAVERSDAALLASKRELDVLGAQLADARARVNTALAAQRVAALNVEYTTIRAPVDGYV
GNRTGRVGMLANVGVPLLTIVPASGLWIDANFKEDQLRKMRAGDRVDVSLDASAARLHGRVDSVAPATGATFSVLPPENA
TGNFTKIVQRVPVRVHLDPQPGLEQVLRPGLSAVVTVHTDHAH

Sequences:

>Translated_363_residues
MDTTQTAAAREAATAEPPAKQRRRVRWMRIAVIAVVVAVGAAALDWLFVLRFQESTDDAYVGGDVTVLAPKVAGFVDRIG
VTDNQRVKAGDVLVQLDARDYAAKLAQASAEVDAARSAVAELEAKQQLQDAVIGQHAADKNASSAELTRAAADRVRYREL
VKADAVSNQLVERADADYAKAHAAVERSDAALLASKRELDVLGAQLADARARVNTALAAQRVAALNVEYTTIRAPVDGYV
GNRTGRVGMLANVGVPLLTIVPASGLWIDANFKEDQLRKMRAGDRVDVSLDASAARLHGRVDSVAPATGATFSVLPPENA
TGNFTKIVQRVPVRVHLDPQPGLEQVLRPGLSAVVTVHTDHAH
>Mature_363_residues
MDTTQTAAAREAATAEPPAKQRRRVRWMRIAVIAVVVAVGAAALDWLFVLRFQESTDDAYVGGDVTVLAPKVAGFVDRIG
VTDNQRVKAGDVLVQLDARDYAAKLAQASAEVDAARSAVAELEAKQQLQDAVIGQHAADKNASSAELTRAAADRVRYREL
VKADAVSNQLVERADADYAKAHAAVERSDAALLASKRELDVLGAQLADARARVNTALAAQRVAALNVEYTTIRAPVDGYV
GNRTGRVGMLANVGVPLLTIVPASGLWIDANFKEDQLRKMRAGDRVDVSLDASAARLHGRVDSVAPATGATFSVLPPENA
TGNFTKIVQRVPVRVHLDPQPGLEQVLRPGLSAVVTVHTDHAH

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788711, Length=347, Percent_Identity=32.5648414985591, Blast_Score=168, Evalue=6e-43,
Organism=Escherichia coli, GI1789041, Length=367, Percent_Identity=34.0599455040872, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1790519, Length=324, Percent_Identity=32.7160493827161, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI87081951, Length=316, Percent_Identity=26.2658227848101, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1789637, Length=335, Percent_Identity=26.5671641791045, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790024, Length=309, Percent_Identity=27.1844660194175, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1790012, Length=308, Percent_Identity=26.9480519480519, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 38645; Mature: 38645

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTTQTAAAREAATAEPPAKQRRRVRWMRIAVIAVVVAVGAAALDWLFVLRFQESTDDAY
CCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEE
VGGDVTVLAPKVAGFVDRIGVTDNQRVKAGDVLVQLDARDYAAKLAQASAEVDAARSAVA
ECCCEEEECHHHHHHHHHCCCCCCCEEEECCEEEEECCHHHHHHHHHHHHHHHHHHHHHH
ELEAKQQLQDAVIGQHAADKNASSAELTRAAADRVRYRELVKADAVSNQLVERADADYAK
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
AHAAVERSDAALLASKRELDVLGAQLADARARVNTALAAQRVAALNVEYTTIRAPVDGYV
HHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCC
GNRTGRVGMLANVGVPLLTIVPASGLWIDANFKEDQLRKMRAGDRVDVSLDASAARLHGR
CCCCCCEEEEECCCCCEEEEECCCCEEEECCCCHHHHHHHHCCCCEEEEECCHHHHHHCC
VDSVAPATGATFSVLPPENATGNFTKIVQRVPVRVHLDPQPGLEQVLRPGLSAVVTVHTD
HHCCCCCCCCEEEEECCCCCCCHHHHHHHHCCEEEEECCCCCHHHHHCCCCEEEEEEECC
HAH
CCC
>Mature Secondary Structure
MDTTQTAAAREAATAEPPAKQRRRVRWMRIAVIAVVVAVGAAALDWLFVLRFQESTDDAY
CCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEE
VGGDVTVLAPKVAGFVDRIGVTDNQRVKAGDVLVQLDARDYAAKLAQASAEVDAARSAVA
ECCCEEEECHHHHHHHHHCCCCCCCEEEECCEEEEECCHHHHHHHHHHHHHHHHHHHHHH
ELEAKQQLQDAVIGQHAADKNASSAELTRAAADRVRYRELVKADAVSNQLVERADADYAK
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
AHAAVERSDAALLASKRELDVLGAQLADARARVNTALAAQRVAALNVEYTTIRAPVDGYV
HHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCC
GNRTGRVGMLANVGVPLLTIVPASGLWIDANFKEDQLRKMRAGDRVDVSLDASAARLHGR
CCCCCCEEEEECCCCCEEEEECCCCEEEECCCCHHHHHHHHCCCCEEEEECCHHHHHHCC
VDSVAPATGATFSVLPPENATGNFTKIVQRVPVRVHLDPQPGLEQVLRPGLSAVVTVHTD
HHCCCCCCCCEEEEECCCCCCCHHHHHHHHCCEEEEECCCCCHHHHHCCCCEEEEEEECC
HAH
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]