Click here to switch to the map view.

The map label for this gene is gcvA [H]

Identifier: 189352308

GI number: 189352308

Start: 357112

End: 357999

Strand: Reverse

Name: gcvA [H]

Synonym: BMULJ_03527

Alternate gene names: 189352308

Gene position: 357999-357112 (Counterclockwise)

Preceding gene: 189352309

Following gene: 189352307

Centisome position: 14.48

GC content: 65.65

Gene sequence:

>888_bases
ATGCGTATCCCTCCGCTGAAATCCATCATCGCGTTCGAGAGCGTCGCGCGCACCAAAAGCGTGAACCGCGCGGCGGAGGA
ACTCGGGCTGACCGCGTCGGCCGTCAGCCATCAGCTGAGCAATCTCGAAGCGCTGATCGGCCGGCCGCTGTTTCAGCGCT
CGGGCCGCGGGCTCGTGCTGACGCCGACCGGCGAACGCTATCTGGCCGACGTGACGGGCTCGCTCGCCGACCTGAGCCGC
GCGACCGAACGCGCGTCGAGCCGCACCGACGTCGATATCCTGCGCGTGCATTCGAGCCCGAGCTTCGGGCTCATGTGGCT
GCTGCCGCGGCTGTCGTCGTTTCAGGAGGCCAACGGCGATCTGCAGCTCAATCTCGCCTGCTCCTATGAAAACGTGTCGT
TCTCGAACGGCTACTACGACATCGACATCCGGCACGGCTACGGCAACTGGGACAACCTCGAAGTGAAGACCGTGCGCGGC
GAATACATCGCGCCGCTCGCGTCGCCCGCCTATCTCGAGCGGCATCCGGTCCGCACGCCCGAAGATCTGCTCGCGCATCG
TCTGATCTATTCGGAGACGCCGCTCGTGCAGTGGAAGCAGTGGTTCGGCCGAACCGGCGTGTCGGCCAAGCAGAAGACCT
TCGACTTCTCGTTCGACCGCTCGTACATGTCGATCGAGACGGCCGCGCTCGGCCTCGGCATCGCGCTCGAAAGCCTCACG
CTGGCGTCGGTGAAGATCCGCGACGGGCTGCTCGTGCCCGTTTTCGACGACAGCTATGCCGTCGAGGTCGGCGCGCATCA
TCTGGTTTACCCGAGCCAGAATGCGGCGCTGCCGCGCATCCAGCGCTTCCTCGCATGGATCGAACGGGAGGCGGCCGCCT
CCACCTGA

Upstream 100 bases:

>100_bases
GATCTGCGGCGAATGTTGTGCGACGCATCACAGCGGATGCGCGCGACGTCGCCACACACCCGACGCGATTCCGATCAACC
GCAACCGAAAAGACGTCACG

Downstream 100 bases:

>100_bases
ATTTTTCTCAGCAATGGTTGAGCGTTTCCGCGTTGATGGTGCCGCGTCGTCCCCCGACACTACAGGCACTCATCGACACC
CAGGAGACAAACGATGTTGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MRIPPLKSIIAFESVARTKSVNRAAEELGLTASAVSHQLSNLEALIGRPLFQRSGRGLVLTPTGERYLADVTGSLADLSR
ATERASSRTDVDILRVHSSPSFGLMWLLPRLSSFQEANGDLQLNLACSYENVSFSNGYYDIDIRHGYGNWDNLEVKTVRG
EYIAPLASPAYLERHPVRTPEDLLAHRLIYSETPLVQWKQWFGRTGVSAKQKTFDFSFDRSYMSIETAALGLGIALESLT
LASVKIRDGLLVPVFDDSYAVEVGAHHLVYPSQNAALPRIQRFLAWIEREAAAST

Sequences:

>Translated_295_residues
MRIPPLKSIIAFESVARTKSVNRAAEELGLTASAVSHQLSNLEALIGRPLFQRSGRGLVLTPTGERYLADVTGSLADLSR
ATERASSRTDVDILRVHSSPSFGLMWLLPRLSSFQEANGDLQLNLACSYENVSFSNGYYDIDIRHGYGNWDNLEVKTVRG
EYIAPLASPAYLERHPVRTPEDLLAHRLIYSETPLVQWKQWFGRTGVSAKQKTFDFSFDRSYMSIETAALGLGIALESLT
LASVKIRDGLLVPVFDDSYAVEVGAHHLVYPSQNAALPRIQRFLAWIEREAAAST
>Mature_295_residues
MRIPPLKSIIAFESVARTKSVNRAAEELGLTASAVSHQLSNLEALIGRPLFQRSGRGLVLTPTGERYLADVTGSLADLSR
ATERASSRTDVDILRVHSSPSFGLMWLLPRLSSFQEANGDLQLNLACSYENVSFSNGYYDIDIRHGYGNWDNLEVKTVRG
EYIAPLASPAYLERHPVRTPEDLLAHRLIYSETPLVQWKQWFGRTGVSAKQKTFDFSFDRSYMSIETAALGLGIALESLT
LASVKIRDGLLVPVFDDSYAVEVGAHHLVYPSQNAALPRIQRFLAWIEREAAAST

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=293, Percent_Identity=35.1535836177474, Blast_Score=163, Evalue=1e-41,
Organism=Escherichia coli, GI1786448, Length=287, Percent_Identity=35.5400696864112, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1788706, Length=297, Percent_Identity=27.6094276094276, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1786401, Length=279, Percent_Identity=27.5985663082437, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI145693193, Length=292, Percent_Identity=27.7397260273973, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1790011, Length=262, Percent_Identity=25.9541984732824, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787589, Length=290, Percent_Identity=24.4827586206897, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32727; Mature: 32727

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIPPLKSIIAFESVARTKSVNRAAEELGLTASAVSHQLSNLEALIGRPLFQRSGRGLVL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHCCCEEEE
TPTGERYLADVTGSLADLSRATERASSRTDVDILRVHSSPSFGLMWLLPRLSSFQEANGD
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCC
LQLNLACSYENVSFSNGYYDIDIRHGYGNWDNLEVKTVRGEYIAPLASPAYLERHPVRTP
EEEEEEEEECCCEECCCEEEEEEEECCCCCCCEEEEEECCCEECCCCCCHHHHCCCCCCH
EDLLAHRLIYSETPLVQWKQWFGRTGVSAKQKTFDFSFDRSYMSIETAALGLGIALESLT
HHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHEECCEECCCHHHHHHHHHHHHHHHHHHE
LASVKIRDGLLVPVFDDSYAVEVGAHHLVYPSQNAALPRIQRFLAWIEREAAAST
EEEEEECCCEEEEEECCCEEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRIPPLKSIIAFESVARTKSVNRAAEELGLTASAVSHQLSNLEALIGRPLFQRSGRGLVL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHCCCEEEE
TPTGERYLADVTGSLADLSRATERASSRTDVDILRVHSSPSFGLMWLLPRLSSFQEANGD
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCC
LQLNLACSYENVSFSNGYYDIDIRHGYGNWDNLEVKTVRGEYIAPLASPAYLERHPVRTP
EEEEEEEEECCCEECCCEEEEEEEECCCCCCCEEEEEECCCEECCCCCCHHHHCCCCCCH
EDLLAHRLIYSETPLVQWKQWFGRTGVSAKQKTFDFSFDRSYMSIETAALGLGIALESLT
HHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHEECCEECCCHHHHHHHHHHHHHHHHHHE
LASVKIRDGLLVPVFDDSYAVEVGAHHLVYPSQNAALPRIQRFLAWIEREAAAST
EEEEEECCCEEEEEECCCEEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]