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The map label for this gene is vanB [H]

Identifier: 189352226

GI number: 189352226

Start: 265183

End: 266163

Strand: Reverse

Name: vanB [H]

Synonym: BMULJ_03444

Alternate gene names: 189352226

Gene position: 266163-265183 (Counterclockwise)

Preceding gene: 189352227

Following gene: 189352219

Centisome position: 10.76

GC content: 69.62

Gene sequence:

>981_bases
ATGCAAGCCAATCGCCACCAGGTCCGGATCGACGCGCTGATCGACGCGGCGCAGGACATCCGCTGTTTCCGCGTGTCGCG
CGTCGACGGCCAGCCGCTCGACGCGTACGAACCGGGCGCCCATATCGACGTCACCGCGCCGTCCGGCGTCACGCGCCAAT
ACTCGTTGTGCGGCCGCCCCGACGAGCGCGGCAGCTATTTGTTCGCCGTGAAGAAGGAAACGCAGTCGCGCGGCGGATCG
CGTTCGCTGCACGACGACGTCCGCGTCGGCACCGAGCTGTCGATCGGCACGCCGCGCAACCTGTTCCGGCTCGCCGACGA
CGCGAGCGAACACGTGCTGATCGCCGCCGGCATCGGCATCACGCCGCTGCTGTCGATGGCGTATGCGCTCGCGCAGCGCG
GCGCCCGCTACCGGCTCCACTATTTCGCGCGCAGCCGCGACCGTGCGGCGTTCGTCGACGAACTGTCGGCCGAACCGTTC
GCGCCGCACGTGACGTTCCATTACGGCGTCGAGCGCGAGGCGCTCGCGCGCGAACTCGGTCGCTGCGTCGAATCGATCGA
CGCGCAGGCACACGTGTACACCTGCGGCCCTGCGCCGTTCATGGACGCGGTGGTTGCCGCCGCCGCGACGCGCGTGCCGG
ACGACGCGATCCATCTCGAGCGCTTCGCGGCCGAACCGGCTGTCGCCGGCGACGCCGCGAACGCAAACGCATCCGAAGGC
TTCGAGGTGCGGCTACAACGCAGCGGCCAGTCCGTGCGCGTCGCGCCCGACACGTCGATCGTCGACGCGCTCGCGCGTAT
CGGCATCGAAGTCGACACGTCGTGCGGCGAAGGGGTGTGCGGCACCTGCATGGTGCCGGTCGTCGACGGCGAGCCCGACC
ACCGCGATCACTGCCTCAGCAAGGCCGAACGCGCGAGCAATTCGGTGATCTGCTGCTGCGTGTCGCGCGCGCGTTCGCCG
GTGCTCGTGCTCGATCTATAA

Upstream 100 bases:

>100_bases
TTTAATCGATTTGATTTATGCGATGCCGCTCCCTATTCTCGATCGCATCACGGCGCGACGGGTGTCGCGCCGGCTTTCCC
GATCCCCACGGAATCGACTC

Downstream 100 bases:

>100_bases
ACGGCGCGCGGCCGCGAGCGCGTCACGCGCCGTCGAAACGCTCGACGATCTCCGCAAGCAGCGCACGCATCCACGTAATG
CCTTCGTCCTCGTGGAAATG

Product: phthalate 4,5-dioxygenase

Products: 3,4-dihydroxybenzoate; NAD+; H2O; formaldehyde

Alternate protein names: Vanillate degradation ferredoxin-like protein [H]

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MQANRHQVRIDALIDAAQDIRCFRVSRVDGQPLDAYEPGAHIDVTAPSGVTRQYSLCGRPDERGSYLFAVKKETQSRGGS
RSLHDDVRVGTELSIGTPRNLFRLADDASEHVLIAAGIGITPLLSMAYALAQRGARYRLHYFARSRDRAAFVDELSAEPF
APHVTFHYGVEREALARELGRCVESIDAQAHVYTCGPAPFMDAVVAAAATRVPDDAIHLERFAAEPAVAGDAANANASEG
FEVRLQRSGQSVRVAPDTSIVDALARIGIEVDTSCGEGVCGTCMVPVVDGEPDHRDHCLSKAERASNSVICCCVSRARSP
VLVLDL

Sequences:

>Translated_326_residues
MQANRHQVRIDALIDAAQDIRCFRVSRVDGQPLDAYEPGAHIDVTAPSGVTRQYSLCGRPDERGSYLFAVKKETQSRGGS
RSLHDDVRVGTELSIGTPRNLFRLADDASEHVLIAAGIGITPLLSMAYALAQRGARYRLHYFARSRDRAAFVDELSAEPF
APHVTFHYGVEREALARELGRCVESIDAQAHVYTCGPAPFMDAVVAAAATRVPDDAIHLERFAAEPAVAGDAANANASEG
FEVRLQRSGQSVRVAPDTSIVDALARIGIEVDTSCGEGVCGTCMVPVVDGEPDHRDHCLSKAERASNSVICCCVSRARSP
VLVLDL
>Mature_326_residues
MQANRHQVRIDALIDAAQDIRCFRVSRVDGQPLDAYEPGAHIDVTAPSGVTRQYSLCGRPDERGSYLFAVKKETQSRGGS
RSLHDDVRVGTELSIGTPRNLFRLADDASEHVLIAAGIGITPLLSMAYALAQRGARYRLHYFARSRDRAAFVDELSAEPF
APHVTFHYGVEREALARELGRCVESIDAQAHVYTCGPAPFMDAVVAAAATRVPDDAIHLERFAAEPAVAGDAANANASEG
FEVRLQRSGQSVRVAPDTSIVDALARIGIEVDTSCGEGVCGTCMVPVVDGEPDHRDHCLSKAERASNSVICCCVSRARSP
VLVLDL

Specific function: Unknown

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788104, Length=325, Percent_Identity=38.4615384615385, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1787658, Length=324, Percent_Identity=28.3950617283951, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1787098, Length=282, Percent_Identity=30.1418439716312, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788903, Length=230, Percent_Identity=31.7391304347826, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR001433
- InterPro:   IPR000951
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.14.13.82

Molecular weight: Translated: 35140; Mature: 35140

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQANRHQVRIDALIDAAQDIRCFRVSRVDGQPLDAYEPGAHIDVTAPSGVTRQYSLCGRP
CCCCCCEEEHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCEEEEECCCCCCEEHHCCCCC
DERGSYLFAVKKETQSRGGSRSLHDDVRVGTELSIGTPRNLFRLADDASEHVLIAAGIGI
CCCCCEEEEEECCHHCCCCCCCCCHHHHCCCEEECCCCHHHHHHHCCCCCCEEEEECCCH
TPLLSMAYALAQRGARYRLHYFARSRDRAAFVDELSAEPFAPHVTFHYGVEREALARELG
HHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHH
RCVESIDAQAHVYTCGPAPFMDAVVAAAATRVPDDAIHLERFAAEPAVAGDAANANASEG
HHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCC
FEVRLQRSGQSVRVAPDTSIVDALARIGIEVDTSCGEGVCGTCMVPVVDGEPDHRDHCLS
EEEEEECCCCEEEECCCHHHHHHHHHCCCEEECCCCCCCCCEEEEEEECCCCCHHHHHHH
KAERASNSVICCCVSRARSPVLVLDL
HHHHCCCCEEEEEEHHCCCCEEEEEC
>Mature Secondary Structure
MQANRHQVRIDALIDAAQDIRCFRVSRVDGQPLDAYEPGAHIDVTAPSGVTRQYSLCGRP
CCCCCCEEEHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCEEEEECCCCCCEEHHCCCCC
DERGSYLFAVKKETQSRGGSRSLHDDVRVGTELSIGTPRNLFRLADDASEHVLIAAGIGI
CCCCCEEEEEECCHHCCCCCCCCCHHHHCCCEEECCCCHHHHHHHCCCCCCEEEEECCCH
TPLLSMAYALAQRGARYRLHYFARSRDRAAFVDELSAEPFAPHVTFHYGVEREALARELG
HHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHH
RCVESIDAQAHVYTCGPAPFMDAVVAAAATRVPDDAIHLERFAAEPAVAGDAANANASEG
HHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCC
FEVRLQRSGQSVRVAPDTSIVDALARIGIEVDTSCGEGVCGTCMVPVVDGEPDHRDHCLS
EEEEEECCCCEEEECCCHHHHHHHHHCCCEEECCCCCCCCCEEEEEEECCCCCHHHHHHH
KAERASNSVICCCVSRARSPVLVLDL
HHHHCCCCEEEEEEHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: vanillate; O2; NADH; H+

Specific reaction: vanillate + O2 + NADH + H+ = 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9098058 [H]