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The map label for this gene is xylX [H]

Identifier: 189352219

GI number: 189352219

Start: 257657

End: 258940

Strand: Reverse

Name: xylX [H]

Synonym: BMULJ_03437

Alternate gene names: 189352219

Gene position: 258940-257657 (Counterclockwise)

Preceding gene: 189352226

Following gene: 189352218

Centisome position: 10.47

GC content: 65.65

Gene sequence:

>1284_bases
ATGAACGACATTCCGTATCAGACGATCGACACGCGTGCGCTGCACGGTCTCGCGCAACCCGACCGCATCGCACCCGCGCT
CTATCACGACCCGGCGCTGTTCGAAGCCGAGCTCGACCGCATCTTCTACCGCACCTGGATCTGGGTCGCGCACGATAGCG
AATTGCCGCGCCCCGGCGACTTCATCACGACGACGATCGGCCGCCAACCGGTGATCGTCGTGCGCGACAAGACCGGCGAA
GTGAACGTGCTGCAGAACCGCTGCCGCCATCGCGGCGCGACCGTCTGCGAAGCGCACAAGGGCAACGCGAAAGGCTTCAC
GTGCCCGTATCACAGCTGGTCGTACGCACTCGACGGCACGCTGCGCGCGCTGCCGTACGGCGACGGCTACGAAGGCGTGT
GCGACAAGGGCGATCTGCCGCTCGTGAAGCTGCGCGTCGGCGTGTATCAGGGGCTGATCTTCGCGAGCTTCAACGACGAG
ATCGAACCGCTCGAGGACTTTCTCGGCGGCGCGAAGCCGTGGATCGACCTGTTCATGAAGCAGGGCGCCGGCTATCCGAT
CAAGGCCAACGGCGAACACAAGTTCCGCTTCAAGGGCAACTGGAAGATCCAGCTCGAGAACACGACCGATCTCTATCACT
TTCCGGTCGTGCACAAGTCGTGGATGGAGTCGATCGACGACGAGACGGCCGCCGCGATCACAAGCTTCATGACGAGCGAC
GCCGCGTTCTGCCGTTCGCTCGGCAACGGCCACAGCCTCGCGGTGCTGATGCCGGAGCTCGTCGATCTCGACGACGACGA
CGGCGCACCGCTGCCCGAGCGCTTTGCGCCGCTCGCCGCGAAGCTCGCCGAGCGGCACACGCCGGAACAAGTGCGGCGCA
TCGTGCGCTCGCTGATGGGCGTCGGCTTCAACCTGAACCTGTTTCCGAATCTCGCGCTGTCGATGGCGTTCTTCCGCGTG
CTGCGGCCGATCTCCGCAACCGAAACCGAGATCCGTCACGTCGCGCTCGCGATGGACGGCGGCCCCGACGAAGCGAACCG
CGAGCGGCTGCGGATCCACGAGCACTTCCAGGGCCCGTTCGGCTTCGGCAGCCCGGACGACGCCGAAGCGTGGGAGCGCG
TGCAGCGCGGCGCGTATGCGGGCCCCGACGTGCCGATTCTCGTGAACCGCGGACTGAACCGCGAGACCATCGCCGCAAAC
GGCGAAAAGACCGCGCACGCGACCGACGAAACCGGCATGCGCGAAGCGTATCGGCAATGGCGGACGATGATGGAGCAAGC
ATGA

Upstream 100 bases:

>100_bases
ATTTGACCGATGCGCAGGCGGCGACGAAACTGGCTTCGTGATCCGCCCGAGGCACGCACGCCGACGTCTCGGTCCCTCGC
ACTCTTTCCCCACGACGACG

Downstream 100 bases:

>100_bases
TGGACGACCGCAACGCACTCTTCTCGCAGCAGACCTTCGCGCGCGCGGTCGAATTCGTATGGCGCGAAGCCGAGATGCTC
GACCGCCGCGACTATCGCGC

Product: ring-hydroxylating dioxygenase large terminal subunit

Products: NAD; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol [C]

Alternate protein names: NA

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MNDIPYQTIDTRALHGLAQPDRIAPALYHDPALFEAELDRIFYRTWIWVAHDSELPRPGDFITTTIGRQPVIVVRDKTGE
VNVLQNRCRHRGATVCEAHKGNAKGFTCPYHSWSYALDGTLRALPYGDGYEGVCDKGDLPLVKLRVGVYQGLIFASFNDE
IEPLEDFLGGAKPWIDLFMKQGAGYPIKANGEHKFRFKGNWKIQLENTTDLYHFPVVHKSWMESIDDETAAAITSFMTSD
AAFCRSLGNGHSLAVLMPELVDLDDDDGAPLPERFAPLAAKLAERHTPEQVRRIVRSLMGVGFNLNLFPNLALSMAFFRV
LRPISATETEIRHVALAMDGGPDEANRERLRIHEHFQGPFGFGSPDDAEAWERVQRGAYAGPDVPILVNRGLNRETIAAN
GEKTAHATDETGMREAYRQWRTMMEQA

Sequences:

>Translated_427_residues
MNDIPYQTIDTRALHGLAQPDRIAPALYHDPALFEAELDRIFYRTWIWVAHDSELPRPGDFITTTIGRQPVIVVRDKTGE
VNVLQNRCRHRGATVCEAHKGNAKGFTCPYHSWSYALDGTLRALPYGDGYEGVCDKGDLPLVKLRVGVYQGLIFASFNDE
IEPLEDFLGGAKPWIDLFMKQGAGYPIKANGEHKFRFKGNWKIQLENTTDLYHFPVVHKSWMESIDDETAAAITSFMTSD
AAFCRSLGNGHSLAVLMPELVDLDDDDGAPLPERFAPLAAKLAERHTPEQVRRIVRSLMGVGFNLNLFPNLALSMAFFRV
LRPISATETEIRHVALAMDGGPDEANRERLRIHEHFQGPFGFGSPDDAEAWERVQRGAYAGPDVPILVNRGLNRETIAAN
GEKTAHATDETGMREAYRQWRTMMEQA
>Mature_427_residues
MNDIPYQTIDTRALHGLAQPDRIAPALYHDPALFEAELDRIFYRTWIWVAHDSELPRPGDFITTTIGRQPVIVVRDKTGE
VNVLQNRCRHRGATVCEAHKGNAKGFTCPYHSWSYALDGTLRALPYGDGYEGVCDKGDLPLVKLRVGVYQGLIFASFNDE
IEPLEDFLGGAKPWIDLFMKQGAGYPIKANGEHKFRFKGNWKIQLENTTDLYHFPVVHKSWMESIDDETAAAITSFMTSD
AAFCRSLGNGHSLAVLMPELVDLDDDDGAPLPERFAPLAAKLAERHTPEQVRRIVRSLMGVGFNLNLFPNLALSMAFFRV
LRPISATETEIRHVALAMDGGPDEANRERLRIHEHFQGPFGFGSPDDAEAWERVQRGAYAGPDVPILVNRGLNRETIAAN
GEKTAHATDETGMREAYRQWRTMMEQA

Specific function: Part Of The Multicomponent 3-Phenylpropionate Dioxygenase. Converts 3-Phenylpropionic Acid (Pp) Into Cis-3-(3- Carboxyethyl)-3,5-Cyclohexadiene-1,2-Diol (Pp-Dihydrodiol). [C]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788888, Length=453, Percent_Identity=28.9183222958057, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1788103, Length=205, Percent_Identity=28.2926829268293, Blast_Score=93, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017639
- InterPro:   IPR017941
- InterPro:   IPR015881
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: 1.14.1.- [C]

Molecular weight: Translated: 47892; Mature: 47892

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS00570 RING_HYDROXYL_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDIPYQTIDTRALHGLAQPDRIAPALYHDPALFEAELDRIFYRTWIWVAHDSELPRPGD
CCCCCCCCHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHEEEEEECCCCCCCCC
FITTTIGRQPVIVVRDKTGEVNVLQNRCRHRGATVCEAHKGNAKGFTCPYHSWSYALDGT
EEEEECCCCCEEEEECCCCCHHHHHHHHHHCCCCEEECCCCCCCCEECCCCCCCEEECCE
LRALPYGDGYEGVCDKGDLPLVKLRVGVYQGLIFASFNDEIEPLEDFLGGAKPWIDLFMK
EEECCCCCCCCCCCCCCCCCEEEEEHHHHHCEEEEECCCCHHHHHHHHCCCCHHHHHHHH
QGAGYPIKANGEHKFRFKGNWKIQLENTTDLYHFPVVHKSWMESIDDETAAAITSFMTSD
CCCCCCEECCCCEEEEEECCEEEEECCCCCEEEECHHHHHHHHHHCHHHHHHHHHHHHHH
AAFCRSLGNGHSLAVLMPELVDLDDDDGAPLPERFAPLAAKLAERHTPEQVRRIVRSLMG
HHHHHHCCCCCEEEEECHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHC
VGFNLNLFPNLALSMAFFRVLRPISATETEIRHVALAMDGGPDEANRERLRIHEHFQGPF
CCCCEEECHHHHHHHHHHHHHCCCCCCHHHEEEEEEEECCCCCCCCHHHHHHHHHCCCCC
GFGSPDDAEAWERVQRGAYAGPDVPILVNRGLNRETIAANGEKTAHATDETGMREAYRQW
CCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCEEECCCCCCCCCCCHHHHHHHHHHH
RTMMEQA
HHHHHCC
>Mature Secondary Structure
MNDIPYQTIDTRALHGLAQPDRIAPALYHDPALFEAELDRIFYRTWIWVAHDSELPRPGD
CCCCCCCCHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHEEEEEECCCCCCCCC
FITTTIGRQPVIVVRDKTGEVNVLQNRCRHRGATVCEAHKGNAKGFTCPYHSWSYALDGT
EEEEECCCCCEEEEECCCCCHHHHHHHHHHCCCCEEECCCCCCCCEECCCCCCCEEECCE
LRALPYGDGYEGVCDKGDLPLVKLRVGVYQGLIFASFNDEIEPLEDFLGGAKPWIDLFMK
EEECCCCCCCCCCCCCCCCCEEEEEHHHHHCEEEEECCCCHHHHHHHHCCCCHHHHHHHH
QGAGYPIKANGEHKFRFKGNWKIQLENTTDLYHFPVVHKSWMESIDDETAAAITSFMTSD
CCCCCCEECCCCEEEEEECCEEEEECCCCCEEEECHHHHHHHHHHCHHHHHHHHHHHHHH
AAFCRSLGNGHSLAVLMPELVDLDDDDGAPLPERFAPLAAKLAERHTPEQVRRIVRSLMG
HHHHHHCCCCCEEEEECHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHC
VGFNLNLFPNLALSMAFFRVLRPISATETEIRHVALAMDGGPDEANRERLRIHEHFQGPF
CCCCEEECHHHHHHHHHHHHHCCCCCCHHHEEEEEEEECCCCCCCCHHHHHHHHHCCCCC
GFGSPDDAEAWERVQRGAYAGPDVPILVNRGLNRETIAANGEKTAHATDETGMREAYRQW
CCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCEEECCCCCCCCCCCHHHHHHHHHHH
RTMMEQA
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2Fe-2S. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; Proton; O2; 3-phenylpropionate [C]

Specific reaction: 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD 3-phenylpropionate + NADH + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1740120; 1938949 [H]