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The map label for this gene is ada [H]

Identifier: 189352132

GI number: 189352132

Start: 158250

End: 158783

Strand: Reverse

Name: ada [H]

Synonym: BMULJ_03350

Alternate gene names: 189352132

Gene position: 158783-158250 (Counterclockwise)

Preceding gene: 189352133

Following gene: 189352130

Centisome position: 6.42

GC content: 67.6

Gene sequence:

>534_bases
ATGACGCCCGCGCATCTGATTCCGAGCCCGTTGGGCGACATCGCCGTGCGTATCGAGGACGATGCGCTGACCGGGCTGTT
CTTCGTCGGGCAGAAGTACTATCCGTCGCTCGCGATCGCGCCGGGCGCAGACGTGTACCGTACGTCGCCCGTTGCGCGCA
CGGTCGCCGACGAGATTGCCGAATACTTCGCCGGCGCGCGCACGACGTTCTCGGTGCCGGTCCATCTGCGCGGTACCGCG
TTTCAGCGAAGCGTGTGGAAAGAACTGCTCGCGATTCCGTTCGGCACGCTCGTGTCGTACGGCGAGCTGACCGCGCGCGT
CGGACTACCGGCGAGCGCCGCGCGTGCCGTCGGTGGTGCGGTCGGACGCAACCCCGTGTCGATCATCGTGCCCTGCCATC
GCGTCGTCGGCGCGTCGGGGAGCTTGACCGGGTATGCGGGCGGCATCGAACGCAAGCGCGCGTTGCTGTCGCTTGAAGGT
GCGGGGTTGGGGCATGGGGGGTGGTATTCGGGGCAGCAGGTGCTGGCTTTCTGA

Upstream 100 bases:

>100_bases
GGCCGCCTGTCGCATCATCCTTGCCGCGCACGAGCCGTTGGGCGCCGTGGCGTGCGTATGCGGCGCTGCATGTGTGGCGC
CTTCATGGAGACGCTTTGCG

Downstream 100 bases:

>100_bases
TTGCTTCGGTCGCCGCTCGTCGCTCGCCGCGTGTGTTCCCTACTCCCCGCCGCGCTTGCGCATCGCATGTCCGACGCGTG
CATTTGTCTCGAATTTCGCG

Product: methylated-DNA-[protein]-cysteine S-methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 177; Mature: 176

Protein sequence:

>177_residues
MTPAHLIPSPLGDIAVRIEDDALTGLFFVGQKYYPSLAIAPGADVYRTSPVARTVADEIAEYFAGARTTFSVPVHLRGTA
FQRSVWKELLAIPFGTLVSYGELTARVGLPASAARAVGGAVGRNPVSIIVPCHRVVGASGSLTGYAGGIERKRALLSLEG
AGLGHGGWYSGQQVLAF

Sequences:

>Translated_177_residues
MTPAHLIPSPLGDIAVRIEDDALTGLFFVGQKYYPSLAIAPGADVYRTSPVARTVADEIAEYFAGARTTFSVPVHLRGTA
FQRSVWKELLAIPFGTLVSYGELTARVGLPASAARAVGGAVGRNPVSIIVPCHRVVGASGSLTGYAGGIERKRALLSLEG
AGLGHGGWYSGQQVLAF
>Mature_176_residues
TPAHLIPSPLGDIAVRIEDDALTGLFFVGQKYYPSLAIAPGADVYRTSPVARTVADEIAEYFAGARTTFSVPVHLRGTAF
QRSVWKELLAIPFGTLVSYGELTARVGLPASAARAVGGAVGRNPVSIIVPCHRVVGASGSLTGYAGGIERKRALLSLEGA
GLGHGGWYSGQQVLAF

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=166, Percent_Identity=37.9518072289157, Blast_Score=82, Evalue=3e-16,
Organism=Escherichia coli, GI1787596, Length=107, Percent_Identity=52.3364485981308, Blast_Score=113, Evalue=7e-27,
Organism=Escherichia coli, GI1788542, Length=154, Percent_Identity=38.3116883116883, Blast_Score=94, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI115533070, Length=131, Percent_Identity=38.9312977099237, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI115533068, Length=131, Percent_Identity=38.9312977099237, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320001, Length=87, Percent_Identity=47.1264367816092, Blast_Score=89, Evalue=3e-19,
Organism=Drosophila melanogaster, GI17137554, Length=121, Percent_Identity=33.8842975206612, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 18480; Mature: 18349

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPAHLIPSPLGDIAVRIEDDALTGLFFVGQKYYPSLAIAPGADVYRTSPVARTVADEIA
CCCCCCCCCCCCCEEEEECCCCCEEHEEECCCCCCCEEECCCCCCCCCCHHHHHHHHHHH
EYFAGARTTFSVPVHLRGTAFQRSVWKELLAIPFGTLVSYGELTARVGLPASAARAVGGA
HHHCCCCEEEEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHEECCCCHHHHHHHCCC
VGRNPVSIIVPCHRVVGASGSLTGYAGGIERKRALLSLEGAGLGHGGWYSGQQVLAF
CCCCCEEEEEECHHHHCCCCCCCCCCCCHHHHHHEEEECCCCCCCCCCCCCCEEECC
>Mature Secondary Structure 
TPAHLIPSPLGDIAVRIEDDALTGLFFVGQKYYPSLAIAPGADVYRTSPVARTVADEIA
CCCCCCCCCCCCEEEEECCCCCEEHEEECCCCCCCEEECCCCCCCCCCHHHHHHHHHHH
EYFAGARTTFSVPVHLRGTAFQRSVWKELLAIPFGTLVSYGELTARVGLPASAARAVGGA
HHHCCCCEEEEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHEECCCCHHHHHHHCCC
VGRNPVSIIVPCHRVVGASGSLTGYAGGIERKRALLSLEGAGLGHGGWYSGQQVLAF
CCCCCEEEEEECHHHHCCCCCCCCCCCCHHHHHHEEEECCCCCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]