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The map label for this gene is ada [H]

Identifier: 189352133

GI number: 189352133

Start: 158780

End: 160243

Strand: Reverse

Name: ada [H]

Synonym: BMULJ_03351

Alternate gene names: 189352133

Gene position: 160243-158780 (Counterclockwise)

Preceding gene: 189352135

Following gene: 189352132

Centisome position: 6.48

GC content: 71.38

Gene sequence:

>1464_bases
ATGCGCCTCGATCCCGACATCTGCTACGACGCCCTGCTGTCGCGAAACCGCCGCTTCGACGGCTGGTTCTTCGTCGGCGT
GCGCACGACCGGCGTGTACTGCCGCCCCGTTTGTCCCGTGAAGCCGCCGAAAGCGCAGAACTGCCGCTACTTCCCGACCG
CCGCGGCCGCCGAGAAGGCGGGCTTCCGGCCGTGCATGCGTTGCCGCCCCGAGCTCGCGCCGGGCCACGGCCTGCTCGAC
GTGTCGGGCAATCTCGCCAATGCGGCCGCGACATTGATCGACGACGGTTTCCTGAACGACCGCTCCGTCCACGCACTCGC
GCAGCGCGTCGGCGTGACCGAGCGCCATCTGCGCCGGATCTTCGCCGCGCAGTTCGGCGTATCGCCGGTCGAATTCGCGC
AGACGCAGCGGCTGCTGATGGCGAAGCGATTGCTGACCGACACCGCGTTGCCCGTGGCCGTCGTCGCGTCGACGGCCGGG
TTCGGCAGCGTCCGCCGCTTCAACGATCTGTTTCTGCAGCGCTATGGGCTGAACCCGCTGCGCTTGCGCAAAAGCGCTGC
GGCGGGCGCGTCGGCCGGCGGCGACATGACATTCCCGCTGCCGTACCGCCCGCCGTTCGCGTGGCAGGAGCTGATGCGCT
TTCTCGCGCAGCGGCAGATCGACGGTGTCGAATGGATCGACGCGCAGCGCTATCTGCGCGCCGTCGACCTGCCGGCGCCG
CGCGGTGCCGGCCGCGTGACGGGCTGGGTGTCCGTCTCGCACGTGCCGCACCGGTTCGCGCTCGCCGTCACCGTGTCGCC
GGCGCTGACGCCGGTCGTGCCGACCGTGCTCGCGCGCGTGCGGCGGCTGTTCGATCTCGACAGCCGTCCCGACGTGATCG
ACGCGCATCTCGGCGAACTCGCCGACGGCTCGCCGGGTCTGCGCGTGCCGGGCGCGTTCGACGGCGTCGAGATCGCGATC
CGTGCGATCGTCGGCGAACGTCTCGAGACGATGCACGCGGATGCGGTGCTCGCGCGCCTCGCCGCGCACTTCGGCCCACG
CGTGGACAGCGGCGCGCCGCCGACACTGGCACACGCGATGCCTCCTGTGTCGACGCTCGCCGCGCTGCCGCCGGCAGCAC
TTCGCTCGATCGGCGTCGCGCGCGACGCGGCGGAAGCGATCGTTTCGCTCGCGCAGGCGGTCGCCGAAGGCACGCTCGCG
CTCGAACCGATGGCGCCGCTCGACGCGACGCTCGCCGCGTTGCGCGCGATGCCGGGCTTCGACGAGCGCGCGGTGCAGTA
CGTCGCGATGCGCGCAATGGCGTGGCCGAATGCGTTCCCCGCGGATGAGCGGTTGTTGCTTGCGACGCAGGCCGGCGACC
GGCCGCCTGTCGCATCATCCTTGCCGCGCACGAGCCGTTGGGCGCCGTGGCGTGCGTATGCGGCGCTGCATGTGTGGCGC
CTTCATGGAGACGCTTTGCGATGA

Upstream 100 bases:

>100_bases
TTCCGGCGGTTTTCCGGAGATACGCGCGGCGCGCCGGACGACGGATACACATGTCCGATTTCAGCCAGAATCGACGAGGC
GGATGGTTTACAGTCGTCGC

Downstream 100 bases:

>100_bases
CGCCCGCGCATCTGATTCCGAGCCCGTTGGGCGACATCGCCGTGCGTATCGAGGACGATGCGCTGACCGGGCTGTTCTTC
GTCGGGCAGAAGTACTATCC

Product: methylated-DNA-[protein]-cysteine S-methyltransferase

Products: NA

Alternate protein names: Regulatory protein of adaptative response; Methylated-DNA--protein-cysteine methyltransferase; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MRLDPDICYDALLSRNRRFDGWFFVGVRTTGVYCRPVCPVKPPKAQNCRYFPTAAAAEKAGFRPCMRCRPELAPGHGLLD
VSGNLANAAATLIDDGFLNDRSVHALAQRVGVTERHLRRIFAAQFGVSPVEFAQTQRLLMAKRLLTDTALPVAVVASTAG
FGSVRRFNDLFLQRYGLNPLRLRKSAAAGASAGGDMTFPLPYRPPFAWQELMRFLAQRQIDGVEWIDAQRYLRAVDLPAP
RGAGRVTGWVSVSHVPHRFALAVTVSPALTPVVPTVLARVRRLFDLDSRPDVIDAHLGELADGSPGLRVPGAFDGVEIAI
RAIVGERLETMHADAVLARLAAHFGPRVDSGAPPTLAHAMPPVSTLAALPPAALRSIGVARDAAEAIVSLAQAVAEGTLA
LEPMAPLDATLAALRAMPGFDERAVQYVAMRAMAWPNAFPADERLLLATQAGDRPPVASSLPRTSRWAPWRAYAALHVWR
LHGDALR

Sequences:

>Translated_487_residues
MRLDPDICYDALLSRNRRFDGWFFVGVRTTGVYCRPVCPVKPPKAQNCRYFPTAAAAEKAGFRPCMRCRPELAPGHGLLD
VSGNLANAAATLIDDGFLNDRSVHALAQRVGVTERHLRRIFAAQFGVSPVEFAQTQRLLMAKRLLTDTALPVAVVASTAG
FGSVRRFNDLFLQRYGLNPLRLRKSAAAGASAGGDMTFPLPYRPPFAWQELMRFLAQRQIDGVEWIDAQRYLRAVDLPAP
RGAGRVTGWVSVSHVPHRFALAVTVSPALTPVVPTVLARVRRLFDLDSRPDVIDAHLGELADGSPGLRVPGAFDGVEIAI
RAIVGERLETMHADAVLARLAAHFGPRVDSGAPPTLAHAMPPVSTLAALPPAALRSIGVARDAAEAIVSLAQAVAEGTLA
LEPMAPLDATLAALRAMPGFDERAVQYVAMRAMAWPNAFPADERLLLATQAGDRPPVASSLPRTSRWAPWRAYAALHVWR
LHGDALR
>Mature_487_residues
MRLDPDICYDALLSRNRRFDGWFFVGVRTTGVYCRPVCPVKPPKAQNCRYFPTAAAAEKAGFRPCMRCRPELAPGHGLLD
VSGNLANAAATLIDDGFLNDRSVHALAQRVGVTERHLRRIFAAQFGVSPVEFAQTQRLLMAKRLLTDTALPVAVVASTAG
FGSVRRFNDLFLQRYGLNPLRLRKSAAAGASAGGDMTFPLPYRPPFAWQELMRFLAQRQIDGVEWIDAQRYLRAVDLPAP
RGAGRVTGWVSVSHVPHRFALAVTVSPALTPVVPTVLARVRRLFDLDSRPDVIDAHLGELADGSPGLRVPGAFDGVEIAI
RAIVGERLETMHADAVLARLAAHFGPRVDSGAPPTLAHAMPPVSTLAALPPAALRSIGVARDAAEAIVSLAQAVAEGTLA
LEPMAPLDATLAALRAMPGFDERAVQYVAMRAMAWPNAFPADERLLLATQAGDRPPVASSLPRTSRWAPWRAYAALHVWR
LHGDALR

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus [H]

COG id: COG2169

COG function: function code F; Adenosine deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788383, Length=288, Percent_Identity=32.6388888888889, Blast_Score=149, Evalue=6e-37,
Organism=Escherichia coli, GI1788542, Length=163, Percent_Identity=34.3558282208589, Blast_Score=80, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR010316
- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR003583
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR023170
- InterPro:   IPR012294 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF06029 AlkA_N; PF00730 HhH-GPD [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 52725; Mature: 52725

Theoretical pI: Translated: 10.26; Mature: 10.26

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2 ; PS00136 SUBTILASE_ASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDPDICYDALLSRNRRFDGWFFVGVRTTGVYCRPVCPVKPPKAQNCRYFPTAAAAEKA
CCCCHHHHHHHHHHCCCCCCCEEEEEEEECCEEEECCCCCCCCCCCCCCCCCCHHHHHHC
GFRPCMRCRPELAPGHGLLDVSGNLANAAATLIDDGFLNDRSVHALAQRVGVTERHLRRI
CCCHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHH
FAAQFGVSPVEFAQTQRLLMAKRLLTDTALPVAVVASTAGFGSVRRFNDLFLQRYGLNPL
HHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCHH
RLRKSAAAGASAGGDMTFPLPYRPPFAWQELMRFLAQRQIDGVEWIDAQRYLRAVDLPAP
HHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCC
RGAGRVTGWVSVSHVPHRFALAVTVSPALTPVVPTVLARVRRLFDLDSRPDVIDAHLGEL
CCCCCEEEEEEECCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHC
ADGSPGLRVPGAFDGVEIAIRAIVGERLETMHADAVLARLAAHFGPRVDSGAPPTLAHAM
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCC
PPVSTLAALPPAALRSIGVARDAAEAIVSLAQAVAEGTLALEPMAPLDATLAALRAMPGF
CCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHCCCC
DERAVQYVAMRAMAWPNAFPADERLLLATQAGDRPPVASSLPRTSRWAPWRAYAALHVWR
HHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHCCCCCCCCCCHHHHHHHHHHE
LHGDALR
ECCCCCC
>Mature Secondary Structure
MRLDPDICYDALLSRNRRFDGWFFVGVRTTGVYCRPVCPVKPPKAQNCRYFPTAAAAEKA
CCCCHHHHHHHHHHCCCCCCCEEEEEEEECCEEEECCCCCCCCCCCCCCCCCCHHHHHHC
GFRPCMRCRPELAPGHGLLDVSGNLANAAATLIDDGFLNDRSVHALAQRVGVTERHLRRI
CCCHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHH
FAAQFGVSPVEFAQTQRLLMAKRLLTDTALPVAVVASTAGFGSVRRFNDLFLQRYGLNPL
HHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCHH
RLRKSAAAGASAGGDMTFPLPYRPPFAWQELMRFLAQRQIDGVEWIDAQRYLRAVDLPAP
HHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCC
RGAGRVTGWVSVSHVPHRFALAVTVSPALTPVVPTVLARVRRLFDLDSRPDVIDAHLGEL
CCCCCEEEEEEECCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHC
ADGSPGLRVPGAFDGVEIAIRAIVGERLETMHADAVLARLAAHFGPRVDSGAPPTLAHAM
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCC
PPVSTLAALPPAALRSIGVARDAAEAIVSLAQAVAEGTLALEPMAPLDATLAALRAMPGF
CCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHCCCC
DERAVQYVAMRAMAWPNAFPADERLLLATQAGDRPPVASSLPRTSRWAPWRAYAALHVWR
HHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHCCCCCCCCCCHHHHHHHHHHE
LHGDALR
ECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]