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The map label for this gene is nifR3 [H]

Identifier: 189183831

GI number: 189183831

Start: 965068

End: 966030

Strand: Direct

Name: nifR3 [H]

Synonym: OTT_0924

Alternate gene names: 189183831

Gene position: 965068-966030 (Clockwise)

Preceding gene: 189183830

Following gene: 189183833

Centisome position: 48.04

GC content: 34.99

Gene sequence:

>963_bases
ATGACAATAAAAATTGGTAATATTGAATTATCTGCTCCAGTTATTTTAGCTCCAATGTCTGGAGTTACTGATCTGCCATT
TCGCAAACTAGTTAAACAATTTGGAGCAGGGCTAGTAGTTTCAGAAATGGTAGCTAGTAGAGCTATGATTCAAGAAACTC
GTAAATCTATGATGAAATCTGCTATCATTGAGAATGATATTACTGGATCTTGTGTACAGCTCGCCGGCTGTGAGCCAGAT
GTTATAGCTGATGCTGCTAAGATGAATCAGGATATGGGGGCGCAAATTATTGATATTAATTTTGGTTGTCCAGCTAAAAA
AGTAGTAGGAGGATATGCTGGTTCTGCATTAATGCGAGATGAGATATTAGCAGCAAAAATATTAGCTGAAACCGTTAAAA
GCGTTTCTATTCCGGTAACTTTAAAGATGCGTACAGGCTGGGATGTAAGTTGCCGCAATGCTAATATAATAGCTAAAATA
GCAGAAGAGTGTGGCATTCAAATGATTACAGTTCATGGACGTACAAGATGTCAGTTTTATTCAGGTAAATCTGATTGGGC
ATTTATTAGAAAAGTCAAGGAAAGTATAAAAATTCCAGTAGTAGCTAATGGTGATATTACTAGTGTTGATGATGCTATTG
ATTGTCTTAAAGCATCTGGAGCTGATGGAGTTATGATTGGACGTGGAGCATATGGTAAACCATGGCTATTATCACAAATT
TCGCACTATCTAGCTTTTCAAGATTATTTACCATCACCTAGCTTAATAGAGCAAAAACAAATTATTTTAAATCATTATGA
ATCAATATTAAGCTATTATGGTACTGAAGCTGGTATGAAAATAGCACGTAAACATTTAGGATGGTATAGTAAAGGACTGC
CTAATGCAACTGAATTTAGGTCTAAAGTTACTACTATAACAAGTGTAGAGCAGGTTAAGGAGATTATTAATCAATTTTTT
TAA

Upstream 100 bases:

>100_bases
CTGCTTATACTGCATCAGTGAACCATCAAATTAAAATTATCTAACCATTGTTTTTGATTTATAATTTAATTAAAAATTTA
CAATTGGGCGATAAATATTT

Downstream 100 bases:

>100_bases
ATGTAAAATTAATATAATGTTTCATATTATTCACCTATAGATGGATACTTTTTAATTTACTTCTAATAAGATTAGAAATG
TTAGCATTAGTATTTATTAC

Product: NifR3 protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MTIKIGNIELSAPVILAPMSGVTDLPFRKLVKQFGAGLVVSEMVASRAMIQETRKSMMKSAIIENDITGSCVQLAGCEPD
VIADAAKMNQDMGAQIIDINFGCPAKKVVGGYAGSALMRDEILAAKILAETVKSVSIPVTLKMRTGWDVSCRNANIIAKI
AEECGIQMITVHGRTRCQFYSGKSDWAFIRKVKESIKIPVVANGDITSVDDAIDCLKASGADGVMIGRGAYGKPWLLSQI
SHYLAFQDYLPSPSLIEQKQIILNHYESILSYYGTEAGMKIARKHLGWYSKGLPNATEFRSKVTTITSVEQVKEIINQFF

Sequences:

>Translated_320_residues
MTIKIGNIELSAPVILAPMSGVTDLPFRKLVKQFGAGLVVSEMVASRAMIQETRKSMMKSAIIENDITGSCVQLAGCEPD
VIADAAKMNQDMGAQIIDINFGCPAKKVVGGYAGSALMRDEILAAKILAETVKSVSIPVTLKMRTGWDVSCRNANIIAKI
AEECGIQMITVHGRTRCQFYSGKSDWAFIRKVKESIKIPVVANGDITSVDDAIDCLKASGADGVMIGRGAYGKPWLLSQI
SHYLAFQDYLPSPSLIEQKQIILNHYESILSYYGTEAGMKIARKHLGWYSKGLPNATEFRSKVTTITSVEQVKEIINQFF
>Mature_319_residues
TIKIGNIELSAPVILAPMSGVTDLPFRKLVKQFGAGLVVSEMVASRAMIQETRKSMMKSAIIENDITGSCVQLAGCEPDV
IADAAKMNQDMGAQIIDINFGCPAKKVVGGYAGSALMRDEILAAKILAETVKSVSIPVTLKMRTGWDVSCRNANIIAKIA
EECGIQMITVHGRTRCQFYSGKSDWAFIRKVKESIKIPVVANGDITSVDDAIDCLKASGADGVMIGRGAYGKPWLLSQIS
HYLAFQDYLPSPSLIEQKQIILNHYESILSYYGTEAGMKIARKHLGWYSKGLPNATEFRSKVTTITSVEQVKEIINQFF

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=323, Percent_Identity=28.7925696594427, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI239788462, Length=318, Percent_Identity=28.9308176100629, Blast_Score=131, Evalue=9e-31,
Organism=Homo sapiens, GI40807366, Length=228, Percent_Identity=30.7017543859649, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI31742496, Length=230, Percent_Identity=30.4347826086957, Blast_Score=98, Evalue=9e-21,
Organism=Homo sapiens, GI8923374, Length=240, Percent_Identity=30.4166666666667, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI1789660, Length=319, Percent_Identity=43.8871473354232, Blast_Score=279, Evalue=2e-76,
Organism=Escherichia coli, GI1788462, Length=307, Percent_Identity=31.5960912052117, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI145693211, Length=316, Percent_Identity=26.8987341772152, Blast_Score=83, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17543114, Length=282, Percent_Identity=30.8510638297872, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI25144369, Length=227, Percent_Identity=29.9559471365639, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17507177, Length=235, Percent_Identity=27.6595744680851, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17510279, Length=273, Percent_Identity=26.3736263736264, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323433, Length=306, Percent_Identity=27.1241830065359, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6323560, Length=229, Percent_Identity=29.6943231441048, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6323437, Length=239, Percent_Identity=29.7071129707113, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24585320, Length=289, Percent_Identity=30.7958477508651, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24580595, Length=256, Percent_Identity=32.8125, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI19920448, Length=256, Percent_Identity=32.8125, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI19921524, Length=228, Percent_Identity=28.5087719298246, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI45549423, Length=236, Percent_Identity=29.2372881355932, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 34906; Mature: 34775

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIKIGNIELSAPVILAPMSGVTDLPFRKLVKQFGAGLVVSEMVASRAMIQETRKSMMKS
CEEEECCEEECCCEEEECCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
AIIENDITGSCVQLAGCEPDVIADAAKMNQDMGAQIIDINFGCPAKKVVGGYAGSALMRD
HHHCCCCCCCCEEECCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHCCCCCHHHHHH
EILAAKILAETVKSVSIPVTLKMRTGWDVSCRNANIIAKIAEECGIQMITVHGRTRCQFY
HHHHHHHHHHHHHHCCCCEEEEEECCCCEECCCCHHHHHHHHHCCEEEEEECCCEEEEEE
SGKSDWAFIRKVKESIKIPVVANGDITSVDDAIDCLKASGADGVMIGRGAYGKPWLLSQI
CCCCHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHH
SHYLAFQDYLPSPSLIEQKQIILNHYESILSYYGTEAGMKIARKHLGWYSKGLPNATEFR
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHCCCCCHHHHH
SKVTTITSVEQVKEIINQFF
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TIKIGNIELSAPVILAPMSGVTDLPFRKLVKQFGAGLVVSEMVASRAMIQETRKSMMKS
EEEECCEEECCCEEEECCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
AIIENDITGSCVQLAGCEPDVIADAAKMNQDMGAQIIDINFGCPAKKVVGGYAGSALMRD
HHHCCCCCCCCEEECCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHCCCCCHHHHHH
EILAAKILAETVKSVSIPVTLKMRTGWDVSCRNANIIAKIAEECGIQMITVHGRTRCQFY
HHHHHHHHHHHHHHCCCCEEEEEECCCCEECCCCHHHHHHHHHCCEEEEEECCCEEEEEE
SGKSDWAFIRKVKESIKIPVVANGDITSVDDAIDCLKASGADGVMIGRGAYGKPWLLSQI
CCCCHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHH
SHYLAFQDYLPSPSLIEQKQIILNHYESILSYYGTEAGMKIARKHLGWYSKGLPNATEFR
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHCCCCCHHHHH
SKVTTITSVEQVKEIINQFF
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]