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The map label for this gene is ruvC [H]

Identifier: 189183830

GI number: 189183830

Start: 964532

End: 965011

Strand: Direct

Name: ruvC [H]

Synonym: OTT_0923

Alternate gene names: 189183830

Gene position: 964532-965011 (Clockwise)

Preceding gene: 189183829

Following gene: 189183831

Centisome position: 48.01

GC content: 30.21

Gene sequence:

>480_bases
GTGATAATTTTAGGAATAGATCCGTCACTAGTAAGTACTGGTTGGGGAGTAATTAGTATATCAGGTAGCATAGTAAATTA
TATTGATAGTGGAGTTATAAAAACAGTTTCAAAAGATTCGTTAGTTCTAAAGCTAGGGAACATTAGCTTAATGATAGAAA
AATTAATCACTCGTTTTAATCCATTTCATGTAGCAATGGAAGAGGTTTTTATCAATAAAAACTATAGTTCTTCTGTTACT
TTAATTCAAGCTAGAGGAGCTATAATGTCAGTAATAGGTCGTTATAATATTGATTTTAGCGAATATGCTCCTAATAAAAT
TAAAAAAGCAATTGTTGGCGCTGGAAAAGCAGAAAAGCATCAAGTTCAACAAATGGTAAAATTATTAATGCATATTAAGA
AGGCAATTAGCAAAGATGAATCTGATGCTTTAGCTACTGCTTATACTGCATCAGTGAACCATCAAATTAAAATTATCTAA

Upstream 100 bases:

>100_bases
TATTGTTATTAATGAGGATTTAGCTCTCTCTATAAGCAAAAGTGTATCATTTTGTATATGTCAATGATGCGCTTGTTTTG
CTTATTTTATTAATATTGCT

Downstream 100 bases:

>100_bases
CCATTGTTTTTGATTTATAATTTAATTAAAAATTTACAATTGGGCGATAAATATTTATGACAATAAAAATTGGTAATATT
GAATTATCTGCTCCAGTTAT

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC [H]

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MIILGIDPSLVSTGWGVISISGSIVNYIDSGVIKTVSKDSLVLKLGNISLMIEKLITRFNPFHVAMEEVFINKNYSSSVT
LIQARGAIMSVIGRYNIDFSEYAPNKIKKAIVGAGKAEKHQVQQMVKLLMHIKKAISKDESDALATAYTASVNHQIKII

Sequences:

>Translated_159_residues
MIILGIDPSLVSTGWGVISISGSIVNYIDSGVIKTVSKDSLVLKLGNISLMIEKLITRFNPFHVAMEEVFINKNYSSSVT
LIQARGAIMSVIGRYNIDFSEYAPNKIKKAIVGAGKAEKHQVQQMVKLLMHIKKAISKDESDALATAYTASVNHQIKII
>Mature_159_residues
MIILGIDPSLVSTGWGVISISGSIVNYIDSGVIKTVSKDSLVLKLGNISLMIEKLITRFNPFHVAMEEVFINKNYSSSVT
LIQARGAIMSVIGRYNIDFSEYAPNKIKKAIVGAGKAEKHQVQQMVKLLMHIKKAISKDESDALATAYTASVNHQIKII

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family [H]

Homologues:

Organism=Escherichia coli, GI1788170, Length=132, Percent_Identity=42.4242424242424, Blast_Score=97, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR002176 [H]

Pfam domain/function: PF02075 RuvC [H]

EC number: =3.1.22.4 [H]

Molecular weight: Translated: 17392; Mature: 17392

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIILGIDPSLVSTGWGVISISGSIVNYIDSGVIKTVSKDSLVLKLGNISLMIEKLITRFN
CEEECCCCHHHHCCCEEEEECCHHHHHHHCCHHHHCCCCEEEEEECCHHHHHHHHHHHCC
PFHVAMEEVFINKNYSSSVTLIQARGAIMSVIGRYNIDFSEYAPNKIKKAIVGAGKAEKH
HHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHCCCCHHHHCHHHHHHHHHCCCCCHHH
QVQQMVKLLMHIKKAISKDESDALATAYTASVNHQIKII
HHHHHHHHHHHHHHHHCCCCCCHHHHEEEECCCCEEEEC
>Mature Secondary Structure
MIILGIDPSLVSTGWGVISISGSIVNYIDSGVIKTVSKDSLVLKLGNISLMIEKLITRFN
CEEECCCCHHHHCCCEEEEECCHHHHHHHCCHHHHCCCCEEEEEECCHHHHHHHHHHHCC
PFHVAMEEVFINKNYSSSVTLIQARGAIMSVIGRYNIDFSEYAPNKIKKAIVGAGKAEKH
HHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHCCCCHHHHCHHHHHHHHHCCCCCHHH
QVQQMVKLLMHIKKAISKDESDALATAYTASVNHQIKII
HHHHHHHHHHHHHHHHCCCCCCHHHHEEEECCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA