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The map label for this gene is barA [H]

Identifier: 189183162

GI number: 189183162

Start: 248398

End: 249909

Strand: Direct

Name: barA [H]

Synonym: OTT_0255

Alternate gene names: 189183162

Gene position: 248398-249909 (Clockwise)

Preceding gene: 189183149

Following gene: 189183163

Centisome position: 12.36

GC content: 27.38

Gene sequence:

>1512_bases
ATGCATTATAGATATGCGATTAGATTATCATTTATAGCTGTTTTTCTAACTTTAGTAGGCTTAACAATTTATTATAGATA
TAATGTGATCAAATGGTATAGCCTAATTCCTGCACAAAAAAATGCAAGGAATATTGTTTCAAAATATAGAACCAAATTTT
TAAATCAAAATCATAATAAAGATATTATTTCTAAACTTGTAACCAATTCTTATGATTTAAAGTTGGATTTAGATTTAAAA
AATTTAGCTAAACAATCTCTTAATTTCTTTAGGGAAATTGAGTTTATGGAGTCTATAGAACAAATAACATTATATGATAG
TATGAAAAATATTATCATTAGATCAAAAGATGATTATGTTGCTACTAATAATGTTCGTAAAAATATAGGAATTGAAAGTC
TGCTACTTTATCTAGATAAACTGATTTTTTATACATACCAGAAGCCAAATCAAAATCCTACTTTTAAACATGGAGCACGC
ATTACAAACTGCTTTGTTGAATTTGATAATAACACATATCCTTCTAGAGCTAATACTTCTCCATTTTCTGCTAATGTATT
AATTCATTGTGAAAGCCCTATTATTTTAGACCATACAGTAATTGCATTTTTAAAAATTACCTATAATGCTACTCAACAAT
GGATGTACATAAATAATATTAGCAAACAAGTATTAACAATTCTTTTGCTAATGTCTTTAATTTTTTTCTTTATTATAAAA
AGCAGTATATCTAATGTTCAGCAAATTATTAATGTTCAATTGAATGCCAATAAATATCTTGAACAAGCAAGGCAAAAAGC
AGAAAAGGAAAATATAGCTAAAAACAAATTTTTAGCTAATGTTAGTCATGAACTACGAACACCGTTAAGTGCTATTATTG
GACTTACAGAAATAATATTATCTAACTCTCATTGCAAAATAAACTACTACAACTATATTCGTGATATTCATAATTCTGGA
AAGCACTTATTAGCAGTAATTAATGATATTCTTGATTTTTCTAAGGCTTCAGCTAACAAATTAACAGTAGAAAATGTAGC
AGTAGACCTTAACAAATTGGCCGCTTCAAGTTTACGCTTGATGTATACTAAGGCTAAAAAAGCTGGTATAAAACTGATTT
CTAAGTTTCCACCAGATCAAATTATTATTAATGCAGATGCTAAACGTTTAAAACAAGCCTTGTTTAACTTGTTATCTAAT
TCAATAAAATTTACTCCTAGTAAAGGTTCTATAACACTTGAAATTAGTATGAACGAACTAAAGTCTTTAGTTTATATAAA
AGTTATTGATACAGGTATTGGTATTGCGGAACATGATATTCCTAAAGCATTATCAAGTTTTGAACAAGTAAATAATAACC
TAAGTAATCAGCGTGAAGGTACAGGATTAGGCTTACCACTAACGAAGAAGCTCATTTACTTAATGGAAGGAGATTTCGAA
ATAACAAGCAATATAGGACAAGGCACAACTGTTACAATAACATTTAAATATCCTTCCAGAACAATAAATTAA

Upstream 100 bases:

>100_bases
TAATACAACTCTAAATAAATGAAATTAAAATAATTACTGAAGTATTAAAGTGTATAATAATGTACTATTTAATTACAATG
GGCAATATACAAAGAAATAT

Downstream 100 bases:

>100_bases
TATTATAATGGCAAATATATAGGTTATGCTATAGGTATCTAGCATAACCAAAATGATAGAAATGTAAGAGCAAATATAGC
CACACTGCTATAAAACAATT

Product: histidine kinase sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MHYRYAIRLSFIAVFLTLVGLTIYYRYNVIKWYSLIPAQKNARNIVSKYRTKFLNQNHNKDIISKLVTNSYDLKLDLDLK
NLAKQSLNFFREIEFMESIEQITLYDSMKNIIIRSKDDYVATNNVRKNIGIESLLLYLDKLIFYTYQKPNQNPTFKHGAR
ITNCFVEFDNNTYPSRANTSPFSANVLIHCESPIILDHTVIAFLKITYNATQQWMYINNISKQVLTILLLMSLIFFFIIK
SSISNVQQIINVQLNANKYLEQARQKAEKENIAKNKFLANVSHELRTPLSAIIGLTEIILSNSHCKINYYNYIRDIHNSG
KHLLAVINDILDFSKASANKLTVENVAVDLNKLAASSLRLMYTKAKKAGIKLISKFPPDQIIINADAKRLKQALFNLLSN
SIKFTPSKGSITLEISMNELKSLVYIKVIDTGIGIAEHDIPKALSSFEQVNNNLSNQREGTGLGLPLTKKLIYLMEGDFE
ITSNIGQGTTVTITFKYPSRTIN

Sequences:

>Translated_503_residues
MHYRYAIRLSFIAVFLTLVGLTIYYRYNVIKWYSLIPAQKNARNIVSKYRTKFLNQNHNKDIISKLVTNSYDLKLDLDLK
NLAKQSLNFFREIEFMESIEQITLYDSMKNIIIRSKDDYVATNNVRKNIGIESLLLYLDKLIFYTYQKPNQNPTFKHGAR
ITNCFVEFDNNTYPSRANTSPFSANVLIHCESPIILDHTVIAFLKITYNATQQWMYINNISKQVLTILLLMSLIFFFIIK
SSISNVQQIINVQLNANKYLEQARQKAEKENIAKNKFLANVSHELRTPLSAIIGLTEIILSNSHCKINYYNYIRDIHNSG
KHLLAVINDILDFSKASANKLTVENVAVDLNKLAASSLRLMYTKAKKAGIKLISKFPPDQIIINADAKRLKQALFNLLSN
SIKFTPSKGSITLEISMNELKSLVYIKVIDTGIGIAEHDIPKALSSFEQVNNNLSNQREGTGLGLPLTKKLIYLMEGDFE
ITSNIGQGTTVTITFKYPSRTIN
>Mature_503_residues
MHYRYAIRLSFIAVFLTLVGLTIYYRYNVIKWYSLIPAQKNARNIVSKYRTKFLNQNHNKDIISKLVTNSYDLKLDLDLK
NLAKQSLNFFREIEFMESIEQITLYDSMKNIIIRSKDDYVATNNVRKNIGIESLLLYLDKLIFYTYQKPNQNPTFKHGAR
ITNCFVEFDNNTYPSRANTSPFSANVLIHCESPIILDHTVIAFLKITYNATQQWMYINNISKQVLTILLLMSLIFFFIIK
SSISNVQQIINVQLNANKYLEQARQKAEKENIAKNKFLANVSHELRTPLSAIIGLTEIILSNSHCKINYYNYIRDIHNSG
KHLLAVINDILDFSKASANKLTVENVAVDLNKLAASSLRLMYTKAKKAGIKLISKFPPDQIIINADAKRLKQALFNLLSN
SIKFTPSKGSITLEISMNELKSLVYIKVIDTGIGIAEHDIPKALSSFEQVNNNLSNQREGTGLGLPLTKKLIYLMEGDFE
ITSNIGQGTTVTITFKYPSRTIN

Specific function: Member of the two-component regulatory system involved in the regulation of polar organelle development. PleC functions as a membrane-associated protein kinase that transfers phosphate to the response regulator pleD, leading to its activation [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=258, Percent_Identity=34.4961240310078, Blast_Score=142, Evalue=7e-35,
Organism=Escherichia coli, GI48994928, Length=236, Percent_Identity=34.3220338983051, Blast_Score=138, Evalue=9e-34,
Organism=Escherichia coli, GI145693157, Length=261, Percent_Identity=33.7164750957854, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1788713, Length=239, Percent_Identity=31.7991631799163, Blast_Score=125, Evalue=9e-30,
Organism=Escherichia coli, GI87081816, Length=242, Percent_Identity=33.8842975206612, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1786783, Length=305, Percent_Identity=24.9180327868852, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1788549, Length=234, Percent_Identity=29.4871794871795, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1790436, Length=272, Percent_Identity=27.5735294117647, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1786600, Length=231, Percent_Identity=28.1385281385281, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1790861, Length=233, Percent_Identity=27.8969957081545, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1788393, Length=226, Percent_Identity=25.6637168141593, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1790346, Length=211, Percent_Identity=25.5924170616114, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI87082128, Length=302, Percent_Identity=23.5099337748344, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1786912, Length=220, Percent_Identity=25.4545454545455, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 57458; Mature: 57458

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHYRYAIRLSFIAVFLTLVGLTIYYRYNVIKWYSLIPAQKNARNIVSKYRTKFLNQNHNK
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHEEEHHCCCCCHHHHHHHHHHHHHHCCCCCH
DIISKLVTNSYDLKLDLDLKNLAKQSLNFFREIEFMESIEQITLYDSMKNIIIRSKDDYV
HHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEE
ATNNVRKNIGIESLLLYLDKLIFYTYQKPNQNPTFKHGARITNCFVEFDNNTYPSRANTS
ECCCCHHHCCHHHHHHHHHHHHHEEECCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCC
PFSANVLIHCESPIILDHTVIAFLKITYNATQQWMYINNISKQVLTILLLMSLIFFFIIK
CCCCEEEEEECCCEEEHHHHHHHHEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHH
SSISNVQQIINVQLNANKYLEQARQKAEKENIAKNKFLANVSHELRTPLSAIIGLTEIIL
HHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SNSHCKINYYNYIRDIHNSGKHLLAVINDILDFSKASANKLTVENVAVDLNKLAASSLRL
CCCCEEEEHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHH
MYTKAKKAGIKLISKFPPDQIIINADAKRLKQALFNLLSNSIKFTPSKGSITLEISMNEL
HHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCEEECCCCCCEEEEEEHHHH
KSLVYIKVIDTGIGIAEHDIPKALSSFEQVNNNLSNQREGTGLGLPLTKKLIYLMEGDFE
HHEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCEE
ITSNIGQGTTVTITFKYPSRTIN
EECCCCCCEEEEEEEECCCCCCC
>Mature Secondary Structure
MHYRYAIRLSFIAVFLTLVGLTIYYRYNVIKWYSLIPAQKNARNIVSKYRTKFLNQNHNK
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHEEEHHCCCCCHHHHHHHHHHHHHHCCCCCH
DIISKLVTNSYDLKLDLDLKNLAKQSLNFFREIEFMESIEQITLYDSMKNIIIRSKDDYV
HHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEE
ATNNVRKNIGIESLLLYLDKLIFYTYQKPNQNPTFKHGARITNCFVEFDNNTYPSRANTS
ECCCCHHHCCHHHHHHHHHHHHHEEECCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCC
PFSANVLIHCESPIILDHTVIAFLKITYNATQQWMYINNISKQVLTILLLMSLIFFFIIK
CCCCEEEEEECCCEEEHHHHHHHHEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHH
SSISNVQQIINVQLNANKYLEQARQKAEKENIAKNKFLANVSHELRTPLSAIIGLTEIIL
HHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SNSHCKINYYNYIRDIHNSGKHLLAVINDILDFSKASANKLTVENVAVDLNKLAASSLRL
CCCCEEEEHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHH
MYTKAKKAGIKLISKFPPDQIIINADAKRLKQALFNLLSNSIKFTPSKGSITLEISMNEL
HHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCEEECCCCCCEEEEEEHHHH
KSLVYIKVIDTGIGIAEHDIPKALSSFEQVNNNLSNQREGTGLGLPLTKKLIYLMEGDFE
HHEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCEE
ITSNIGQGTTVTITFKYPSRTIN
EECCCCCCEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8421698; 11259647 [H]