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The map label for this gene is dnaB [H]

Identifier: 189183163

GI number: 189183163

Start: 250660

End: 252114

Strand: Direct

Name: dnaB [H]

Synonym: OTT_0256

Alternate gene names: 189183163

Gene position: 250660-252114 (Clockwise)

Preceding gene: 189183162

Following gene: 189183165

Centisome position: 12.48

GC content: 32.78

Gene sequence:

>1455_bases
ATGGAAAAAGATTTACCTATTGCAAAGTCAGCTCCAAGTAATATTCAAGCTGAGCAAATGATACTTGGAGCAGTCCTAAT
TAATAATCGTGTACTATATAGTATTAATGAGTTTTTACTACCTGAACATTTTTATGAGCCATTACATGGCAAAATATACA
CATCGATTAATCTTATTATTAGCAAAGGAATTAGTGCTACTCCAATTTCGCTAAAAAATATGTTATGCAATGAACCTGCA
TTTGATGAAATTGGTGGAGTAAATTATCTTGCAAAACTTACAACTTTAGCACTAAGTATTGTTAATGTAACTGAGTATGG
CAGAATAGTATATGATCTGGCGTTACGACGTTATTTAATTGAAATTGGTGAAAAAATAGTTACAAATGCATATTCTTCTA
CTTTAGCAGATACAGCTATCAGTCAAATAGAAGCTGCTGAATCTCAATTATATGATTTAGCGTCAATAGGAACTTTAAGT
AAAGGATTTATCAAATTACAAACTTCAGTTGAGGAATCATGGGAATCAATTTCTGCTGCCATCAAGAATAAAAACTCAAT
TCATGGTATTAGTAGCGGCTTTCTAGATCTTGATTCTAAGCTTGGAGGGTTTAAAAATTCTGATCTAATCATATTAGCAG
GGAGACCTTCAATGGGGAAAACAGCTCTAGGCGCTAATTTAGCAGTGAATTCTTGCAAATATTTTTTATCATTATCTACT
CAACAAAATAGCAAAGTTTCTAACATAGCTCCATCAGTTGGTTTTTTTTCTTTAGAAATGTCTTCTCAGCAAATTGCAAC
TCGAATCCTTGCTATAGAGTCGGAAATTGATAGTTCTTCCTTATTTAACGGGAAAATTGGTGAACAAGAAGTTAATAAGT
TAAAGAATGTCCAAGAGGCAATACAGAAGTGGAATTTTTACATAGATGATGCTCCTGCAATCTCAATATCAGCAATTAGA
TCACGAGCTCGTAGACTTAAACGCACTCATAATTTAGCAATATTATTTATTGATTATTTGCAGTTAATTAAGATTGATTC
AAATAGAAGTCAATATAATAGAGTAAATGAAATTTCTGAAATTACTCAAAGCCTAAAAGCACTTGCAAAAGAACTTAATA
TTCCAATAATTGCATTATCACAATTATCTAGAGCTGTTGAACAAAGGCCTGATAAAAAGCCGTTGCTATCAGATCTAAGA
GAATCAGGCTCCATTGAACAGGATGCAGATATTGTAATGCTCATATATAGAGAGGAATATTACTTATCTAGATCAGAACC
AGCTCCTGGAACGCCAGAATATACGGAATGGATTGCTAAACAGGATAGATGTCATAATACTGCTGAAATAATTGTTGCTA
AACACCGTAATGGGCCAGTTGGTACAGTGAAGTTACACTATACTAATCAGTATTCTAAATTTGCTAATATGGTTAAGCAC
TCTCTGCAAAGTTAA

Upstream 100 bases:

>100_bases
TAAATGAGTATATCATTTTGGTTTTTGCTATAGTATTTTTAATAAGTAAAAAATAATCTAAGTTTAAGAATATACAAATA
ATTTTAATATTATAAAGAAT

Downstream 100 bases:

>100_bases
TAAAAACCAAGATGTGAATTTAGTACTGCTAACAAAGTAATTTGAAGGATTTTTAGTAAGCAAGAGCACAATGAAAGCAA
GAAAAGAAACGTACAATTTT

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MEKDLPIAKSAPSNIQAEQMILGAVLINNRVLYSINEFLLPEHFYEPLHGKIYTSINLIISKGISATPISLKNMLCNEPA
FDEIGGVNYLAKLTTLALSIVNVTEYGRIVYDLALRRYLIEIGEKIVTNAYSSTLADTAISQIEAAESQLYDLASIGTLS
KGFIKLQTSVEESWESISAAIKNKNSIHGISSGFLDLDSKLGGFKNSDLIILAGRPSMGKTALGANLAVNSCKYFLSLST
QQNSKVSNIAPSVGFFSLEMSSQQIATRILAIESEIDSSSLFNGKIGEQEVNKLKNVQEAIQKWNFYIDDAPAISISAIR
SRARRLKRTHNLAILFIDYLQLIKIDSNRSQYNRVNEISEITQSLKALAKELNIPIIALSQLSRAVEQRPDKKPLLSDLR
ESGSIEQDADIVMLIYREEYYLSRSEPAPGTPEYTEWIAKQDRCHNTAEIIVAKHRNGPVGTVKLHYTNQYSKFANMVKH
SLQS

Sequences:

>Translated_484_residues
MEKDLPIAKSAPSNIQAEQMILGAVLINNRVLYSINEFLLPEHFYEPLHGKIYTSINLIISKGISATPISLKNMLCNEPA
FDEIGGVNYLAKLTTLALSIVNVTEYGRIVYDLALRRYLIEIGEKIVTNAYSSTLADTAISQIEAAESQLYDLASIGTLS
KGFIKLQTSVEESWESISAAIKNKNSIHGISSGFLDLDSKLGGFKNSDLIILAGRPSMGKTALGANLAVNSCKYFLSLST
QQNSKVSNIAPSVGFFSLEMSSQQIATRILAIESEIDSSSLFNGKIGEQEVNKLKNVQEAIQKWNFYIDDAPAISISAIR
SRARRLKRTHNLAILFIDYLQLIKIDSNRSQYNRVNEISEITQSLKALAKELNIPIIALSQLSRAVEQRPDKKPLLSDLR
ESGSIEQDADIVMLIYREEYYLSRSEPAPGTPEYTEWIAKQDRCHNTAEIIVAKHRNGPVGTVKLHYTNQYSKFANMVKH
SLQS
>Mature_484_residues
MEKDLPIAKSAPSNIQAEQMILGAVLINNRVLYSINEFLLPEHFYEPLHGKIYTSINLIISKGISATPISLKNMLCNEPA
FDEIGGVNYLAKLTTLALSIVNVTEYGRIVYDLALRRYLIEIGEKIVTNAYSSTLADTAISQIEAAESQLYDLASIGTLS
KGFIKLQTSVEESWESISAAIKNKNSIHGISSGFLDLDSKLGGFKNSDLIILAGRPSMGKTALGANLAVNSCKYFLSLST
QQNSKVSNIAPSVGFFSLEMSSQQIATRILAIESEIDSSSLFNGKIGEQEVNKLKNVQEAIQKWNFYIDDAPAISISAIR
SRARRLKRTHNLAILFIDYLQLIKIDSNRSQYNRVNEISEITQSLKALAKELNIPIIALSQLSRAVEQRPDKKPLLSDLR
ESGSIEQDADIVMLIYREEYYLSRSEPAPGTPEYTEWIAKQDRCHNTAEIIVAKHRNGPVGTVKLHYTNQYSKFANMVKH
SLQS

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=481, Percent_Identity=35.5509355509356, Blast_Score=284, Evalue=8e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 53757; Mature: 53757

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKDLPIAKSAPSNIQAEQMILGAVLINNRVLYSINEFLLPEHFYEPLHGKIYTSINLII
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEEHHHHHCCHHHHCCCCCCHHHHHHHHH
SKGISATPISLKNMLCNEPAFDEIGGVNYLAKLTTLALSIVNVTEYGRIVYDLALRRYLI
CCCCCCCCHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIGEKIVTNAYSSTLADTAISQIEAAESQLYDLASIGTLSKGFIKLQTSVEESWESISAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHH
IKNKNSIHGISSGFLDLDSKLGGFKNSDLIILAGRPSMGKTALGANLAVNSCKYFLSLST
HHCCCCCCCCCCCCEECHHHCCCCCCCCEEEEECCCCCCCHHHCCCHHHHHEEEEEEECC
QQNSKVSNIAPSVGFFSLEMSSQQIATRILAIESEIDSSSLFNGKIGEQEVNKLKNVQEA
CCCCCHHHCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
IQKWNFYIDDAPAISISAIRSRARRLKRTHNLAILFIDYLQLIKIDSNRSQYNRVNEISE
HHHCCCEECCCCCHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHEECCCHHHHHHHHHHHH
ITQSLKALAKELNIPIIALSQLSRAVEQRPDKKPLLSDLRESGSIEQDADIVMLIYREEY
HHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEEECHH
YLSRSEPAPGTPEYTEWIAKQDRCHNTAEIIVAKHRNGPVGTVKLHYTNQYSKFANMVKH
HCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEEEECCHHHHHHHHHHH
SLQS
HHCC
>Mature Secondary Structure
MEKDLPIAKSAPSNIQAEQMILGAVLINNRVLYSINEFLLPEHFYEPLHGKIYTSINLII
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEEHHHHHCCHHHHCCCCCCHHHHHHHHH
SKGISATPISLKNMLCNEPAFDEIGGVNYLAKLTTLALSIVNVTEYGRIVYDLALRRYLI
CCCCCCCCHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIGEKIVTNAYSSTLADTAISQIEAAESQLYDLASIGTLSKGFIKLQTSVEESWESISAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHH
IKNKNSIHGISSGFLDLDSKLGGFKNSDLIILAGRPSMGKTALGANLAVNSCKYFLSLST
HHCCCCCCCCCCCCEECHHHCCCCCCCCEEEEECCCCCCCHHHCCCHHHHHEEEEEEECC
QQNSKVSNIAPSVGFFSLEMSSQQIATRILAIESEIDSSSLFNGKIGEQEVNKLKNVQEA
CCCCCHHHCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
IQKWNFYIDDAPAISISAIRSRARRLKRTHNLAILFIDYLQLIKIDSNRSQYNRVNEISE
HHHCCCEECCCCCHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHEECCCHHHHHHHHHHHH
ITQSLKALAKELNIPIIALSQLSRAVEQRPDKKPLLSDLRESGSIEQDADIVMLIYREEY
HHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEEECHH
YLSRSEPAPGTPEYTEWIAKQDRCHNTAEIIVAKHRNGPVGTVKLHYTNQYSKFANMVKH
HCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEEEECCHHHHHHHHHHH
SLQS
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA