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The map label for this gene is pcnB [H]

Identifier: 189182930

GI number: 189182930

Start: 29283

End: 30491

Strand: Direct

Name: pcnB [H]

Synonym: OTT_0023

Alternate gene names: 189182930

Gene position: 29283-30491 (Clockwise)

Preceding gene: 189182929

Following gene: 189182931

Centisome position: 1.46

GC content: 28.87

Gene sequence:

>1209_bases
ATGACAGAAATTCCTATCTGCATAATATTTCCATCGCAAAATATTGAAAAGTTATTGCTTATGCTTGCCAGTTCTATAAA
GTCTGCTCGCATTATAGGGGGGGCAATACGTAATATTATATTAGGTCATCCAGTTAATGATATTGACATTGCAACACCAT
TGCTTCCAGAGCAAGTAATGAATTTACTCAAATTAAACAATATAAAGTGTTATGCCACTGGAATTAAGTTTGGTACAGTA
ACAGCTGTAATAAAGCAAGAAAAATTTGAAATTACAACTTTAAGACAAGATATACAGTGCTATGGCAGGTATGCCAAGGT
AGAATTTTCAAATGATTATTTTGAAGATGCAAAAAGGCGTGATTTTACTATTAATGCTCTTAGCTATTCATTTGAGCAGA
GTTTATTATACGATTATTTTAATGGTCTGCAGGATTTAAAAGATAGAAGAGTGATATTTATTGGTGATTCTGATACTCGT
ATTCAAGAAGATGTTTTAAGAATTTTAAGGTTCTTTAGATTTTCATCATATTATGCCAGATCTATCGATCAAGAAGGATA
TCAATCGTGTGCTAAGTATGCTAAAAGCTTATCTATTCTGTCTAAGGAAAGGGTTGTTCAGGAGCTAAGTAAGATTATTA
TTTCTCCTAATGCATATGAAACTTTAGTAAAAATGGCTGAAATTAATATTACATATTATATAGGTCTTAGGTTAAAGTTA
AATTTGTCAGTTTTTAAAAATGTAACAACAATTGCAAAAAAAAAATCCATTTTACTAAATGTTATAGTGCTTTATTCGGT
ATTGCTAAGCAGTAATGATGTACAGGATTTAAAAGAGATTCTTTCAAATTTACGGTTTAGTAAAGCAATGATTAGAAAGA
TAGTTAGGTTATGCCTTTTTTTATCTGAATGTCAAAGTAAAAATGCTATTTTTTTAATAAGAAAATATTGGTTAACCAGG
GCAGATAATATTGATGATTATATTAATTTAGCTTTATTGTTAGGTTGTATTTCTGATGAAGAAGCAAATATGCTTAAGCA
GCAAGTTTATGAATTAATACCTAAATTACCAGTCAGTGGTTCTGATCTTAAAGAACTTGGTTTTAAAAAGCAAGCTATTC
AAGAAGTATTATTATATCTTAATCATGCTTGGATAAACAGTGATTTTACTCTTAATAAGCAAGAATTAATCGATTTATTA
AAGTTATGA

Upstream 100 bases:

>100_bases
ATTGATCAGTCAGAAGGAACTTTTGATGAAGAAACTTATTATTCTTAATAAAACATTAGCCTATTATATAGTGCTGATGT
TTGTTGATATTTTGTAATAC

Downstream 100 bases:

>100_bases
TTTTTTTTAAACTTAAGTTATTTAGGTTAAGTGTAATAGCTTTTTTAATATTTAATTATTGTAGTATTAATATTATTTTT
GCTAAAAAAAAGCTTCGAAT

Product: poly(A) polymerase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MTEIPICIIFPSQNIEKLLLMLASSIKSARIIGGAIRNIILGHPVNDIDIATPLLPEQVMNLLKLNNIKCYATGIKFGTV
TAVIKQEKFEITTLRQDIQCYGRYAKVEFSNDYFEDAKRRDFTINALSYSFEQSLLYDYFNGLQDLKDRRVIFIGDSDTR
IQEDVLRILRFFRFSSYYARSIDQEGYQSCAKYAKSLSILSKERVVQELSKIIISPNAYETLVKMAEINITYYIGLRLKL
NLSVFKNVTTIAKKKSILLNVIVLYSVLLSSNDVQDLKEILSNLRFSKAMIRKIVRLCLFLSECQSKNAIFLIRKYWLTR
ADNIDDYINLALLLGCISDEEANMLKQQVYELIPKLPVSGSDLKELGFKKQAIQEVLLYLNHAWINSDFTLNKQELIDLL
KL

Sequences:

>Translated_402_residues
MTEIPICIIFPSQNIEKLLLMLASSIKSARIIGGAIRNIILGHPVNDIDIATPLLPEQVMNLLKLNNIKCYATGIKFGTV
TAVIKQEKFEITTLRQDIQCYGRYAKVEFSNDYFEDAKRRDFTINALSYSFEQSLLYDYFNGLQDLKDRRVIFIGDSDTR
IQEDVLRILRFFRFSSYYARSIDQEGYQSCAKYAKSLSILSKERVVQELSKIIISPNAYETLVKMAEINITYYIGLRLKL
NLSVFKNVTTIAKKKSILLNVIVLYSVLLSSNDVQDLKEILSNLRFSKAMIRKIVRLCLFLSECQSKNAIFLIRKYWLTR
ADNIDDYINLALLLGCISDEEANMLKQQVYELIPKLPVSGSDLKELGFKKQAIQEVLLYLNHAWINSDFTLNKQELIDLL
KL
>Mature_401_residues
TEIPICIIFPSQNIEKLLLMLASSIKSARIIGGAIRNIILGHPVNDIDIATPLLPEQVMNLLKLNNIKCYATGIKFGTVT
AVIKQEKFEITTLRQDIQCYGRYAKVEFSNDYFEDAKRRDFTINALSYSFEQSLLYDYFNGLQDLKDRRVIFIGDSDTRI
QEDVLRILRFFRFSSYYARSIDQEGYQSCAKYAKSLSILSKERVVQELSKIIISPNAYETLVKMAEINITYYIGLRLKLN
LSVFKNVTTIAKKKSILLNVIVLYSVLLSSNDVQDLKEILSNLRFSKAMIRKIVRLCLFLSECQSKNAIFLIRKYWLTRA
DNIDDYINLALLLGCISDEEANMLKQQVYELIPKLPVSGSDLKELGFKKQAIQEVLLYLNHAWINSDFTLNKQELIDLLK
L

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=409, Percent_Identity=32.7628361858191, Blast_Score=187, Evalue=1e-47,
Organism=Escherichia coli, GI87081691, Length=231, Percent_Identity=29.8701298701299, Blast_Score=107, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71995920, Length=409, Percent_Identity=31.5403422982885, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI281360112, Length=385, Percent_Identity=32.987012987013, Blast_Score=175, Evalue=5e-44,
Organism=Drosophila melanogaster, GI21357337, Length=385, Percent_Identity=32.987012987013, Blast_Score=175, Evalue=5e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 46276; Mature: 46145

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEIPICIIFPSQNIEKLLLMLASSIKSARIIGGAIRNIILGHPVNDIDIATPLLPEQVM
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
NLLKLNNIKCYATGIKFGTVTAVIKQEKFEITTLRQDIQCYGRYAKVEFSNDYFEDAKRR
HHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHCCC
DFTINALSYSFEQSLLYDYFNGLQDLKDRRVIFIGDSDTRIQEDVLRILRFFRFSSYYAR
CEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
SIDQEGYQSCAKYAKSLSILSKERVVQELSKIIISPNAYETLVKMAEINITYYIGLRLKL
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEEEEEEEEEEEEE
NLSVFKNVTTIAKKKSILLNVIVLYSVLLSSNDVQDLKEILSNLRFSKAMIRKIVRLCLF
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSECQSKNAIFLIRKYWLTRADNIDDYINLALLLGCISDEEANMLKQQVYELIPKLPVSG
HHHHCCCCCEEEEHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCC
SDLKELGFKKQAIQEVLLYLNHAWINSDFTLNKQELIDLLKL
CCHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
TEIPICIIFPSQNIEKLLLMLASSIKSARIIGGAIRNIILGHPVNDIDIATPLLPEQVM
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
NLLKLNNIKCYATGIKFGTVTAVIKQEKFEITTLRQDIQCYGRYAKVEFSNDYFEDAKRR
HHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHCCC
DFTINALSYSFEQSLLYDYFNGLQDLKDRRVIFIGDSDTRIQEDVLRILRFFRFSSYYAR
CEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
SIDQEGYQSCAKYAKSLSILSKERVVQELSKIIISPNAYETLVKMAEINITYYIGLRLKL
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEEEEEEEEEEEEE
NLSVFKNVTTIAKKKSILLNVIVLYSVLLSSNDVQDLKEILSNLRFSKAMIRKIVRLCLF
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSECQSKNAIFLIRKYWLTRADNIDDYINLALLLGCISDEEANMLKQQVYELIPKLPVSG
HHHHCCCCCEEEEHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCC
SDLKELGFKKQAIQEVLLYLNHAWINSDFTLNKQELIDLLKL
CCHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA