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The map label for this gene is pfkA [H]

Identifier: 189025770

GI number: 189025770

Start: 584577

End: 586298

Strand: Reverse

Name: pfkA [H]

Synonym: TPASS_0542

Alternate gene names: 189025770

Gene position: 586298-584577 (Counterclockwise)

Preceding gene: 189025771

Following gene: 189025766

Centisome position: 51.45

GC content: 53.08

Gene sequence:

>1722_bases
ATGAGTATTTCTCTCTTGCAACAGGAACGGCATCGCTACCTGCCAAAGGTCCCCGATCTTCTTCGGGGGGACTTTCGGCG
CGTTTGTGCGCGTCGCGGCTTATCCACCACTGCGGTGGCAGACTATGACGCGTTGCGCTCTCTTTTTGCGCGGACCTACG
GTCAGCCGCTGGTGAACTTCGTTAATGCTAGTGAGAAAAACGAAGATTCCCCCATGGAGACTGCACCTGAGCCGCGAGGA
TTACGTGTTGCCATAGTACTCTCAGGGGGTCAGGCACCAGGTGGTCACAACGTTATAGCAGGGCTTTTCGACGGATTAAA
ACGATGGCACGCTGACTCTGTCCTTATCGGTTTTCTAGGCGGTCCTGCGGGCGTGCTTTCTGGCGATCATATCGAGATAT
GCGCAGACCGTGTAGATGCGTACCGCAACACAGGTGGCTTTGACCTCATTGGTTCAGGACGCACCAAAATCGAAAGTGAG
TCCCAGTTTGCCGCCGCGGCGCAGACAGTCACGCGTATGGCGCTTGATGCACTCGTAGTTGTAGGAGGTGATGACTCCAA
CACGAACGCTGCCCTTTTGGCAGAACACTTCGTAAACAGTGGTATTTCTACCAAGGTTATCGGTGTTCCCAAAACTATTG
ACGGAGACTTGAAAAATGAGGCGATAGAAACCTCGTTTGGTTTTGACACTGCCACAAAAACATACAGTGAACTTATCGGG
AACATTGCGCGCGATGCGTGCAGTGCCCGCAAGTACTGGCATTTTATTAAGCTCATGGGGCGTTCAGCCAGTCATATTGC
GCTTGAGTGCGCGCTGAAAACACAGCCAAATGTCTGTCTCATTTCGGAAGAAGTAGCGGCGCAATCGTTAACGCTCGCAC
AGATCGTGCAGTCGTTGTGTGACACCATCGCCACGCGCGCACAACACGGTGAGCACTTTGGTATAGTGTTAGTTCCAGAA
GGACTTATCGAATTTATACCTGAAATGAAGGCTCTAATTACGGAGCTCAACGAGGTGATGGCACGCCGCGCGCAGGAATT
TGAGGCGCTGGACACTCCTGACGCGCAGCGCGTTTGGATAGAGCAGGCGTTGTCTGCTTCTGCTCGTGCGGTGTTTAACG
CATTACCTGCTGAAATTTCCACACAGCTTTTGGCAGATCGGGATCCACATGGGAATGTGCAGGTGTCGCGCATTGACACA
GAGCGTTTGCTCATTCTCCAGGTAACGGAGCGCTTAGCGCAGATGAAGCAGGAAGGTACGTACACCGGTGTTTTTTCCAG
CATTGCTCATTTTTTTGGTTACGAAGGGCGATGCGCTTTTCCGTCCAACTTTGATGCAGATTATTGCTATACGCTCGGTT
TGACCGCGTGTCTTCTGGCCGTGCACAGGTTTACAGGGTACGTAGCGTCGGTGCGTAACTTGACGTCTTCTGTAGCTGAG
TGGGCAGTAGGTGGCGTGCCGCTTACTATGCTCATGAACATGGAGCGCCGACATGGAAGTCAAAAGCCGGTGATAAAAAA
GGCGCTGGTTGACCTGGAGGGCATGCCGTTTCGGGTGTTCTCTCGTCGGCGTGCGAGTTGGGCCTTAAAAACAAGCTACG
TGTATCCGGGGGCGGTACAGTATTATGGACCTCCGGCTGTGTGTGACGAACCTTCAGTAACTATACGCCTTGAGCGGCCC
GCCCCTGCGGCGAATTCCTCATTTGGGCACCGGTCATCCTAG

Upstream 100 bases:

>100_bases
GTATTGCGCATCCACACGCGGTCGCGCCTGCTGCAGAGTGAGTTGTGGGGGTGCGGCGGTGCGGTCGTGCTATTTTTCGT
ATATGAAGGAGTAATCATCG

Downstream 100 bases:

>100_bases
TACAGAAGGGAGCTGAGTGTGTGGTCGCGTTGTCTCCAGCTGCGGTCTACCTTCACTCGTATATCAAGGGAAATATGGTA
GGGGAGGAGTGTGCGTAGCG

Product: diphosphate--fructose-6-phosphate 1-phosphotransferase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 573; Mature: 572

Protein sequence:

>573_residues
MSISLLQQERHRYLPKVPDLLRGDFRRVCARRGLSTTAVADYDALRSLFARTYGQPLVNFVNASEKNEDSPMETAPEPRG
LRVAIVLSGGQAPGGHNVIAGLFDGLKRWHADSVLIGFLGGPAGVLSGDHIEICADRVDAYRNTGGFDLIGSGRTKIESE
SQFAAAAQTVTRMALDALVVVGGDDSNTNAALLAEHFVNSGISTKVIGVPKTIDGDLKNEAIETSFGFDTATKTYSELIG
NIARDACSARKYWHFIKLMGRSASHIALECALKTQPNVCLISEEVAAQSLTLAQIVQSLCDTIATRAQHGEHFGIVLVPE
GLIEFIPEMKALITELNEVMARRAQEFEALDTPDAQRVWIEQALSASARAVFNALPAEISTQLLADRDPHGNVQVSRIDT
ERLLILQVTERLAQMKQEGTYTGVFSSIAHFFGYEGRCAFPSNFDADYCYTLGLTACLLAVHRFTGYVASVRNLTSSVAE
WAVGGVPLTMLMNMERRHGSQKPVIKKALVDLEGMPFRVFSRRRASWALKTSYVYPGAVQYYGPPAVCDEPSVTIRLERP
APAANSSFGHRSS

Sequences:

>Translated_573_residues
MSISLLQQERHRYLPKVPDLLRGDFRRVCARRGLSTTAVADYDALRSLFARTYGQPLVNFVNASEKNEDSPMETAPEPRG
LRVAIVLSGGQAPGGHNVIAGLFDGLKRWHADSVLIGFLGGPAGVLSGDHIEICADRVDAYRNTGGFDLIGSGRTKIESE
SQFAAAAQTVTRMALDALVVVGGDDSNTNAALLAEHFVNSGISTKVIGVPKTIDGDLKNEAIETSFGFDTATKTYSELIG
NIARDACSARKYWHFIKLMGRSASHIALECALKTQPNVCLISEEVAAQSLTLAQIVQSLCDTIATRAQHGEHFGIVLVPE
GLIEFIPEMKALITELNEVMARRAQEFEALDTPDAQRVWIEQALSASARAVFNALPAEISTQLLADRDPHGNVQVSRIDT
ERLLILQVTERLAQMKQEGTYTGVFSSIAHFFGYEGRCAFPSNFDADYCYTLGLTACLLAVHRFTGYVASVRNLTSSVAE
WAVGGVPLTMLMNMERRHGSQKPVIKKALVDLEGMPFRVFSRRRASWALKTSYVYPGAVQYYGPPAVCDEPSVTIRLERP
APAANSSFGHRSS
>Mature_572_residues
SISLLQQERHRYLPKVPDLLRGDFRRVCARRGLSTTAVADYDALRSLFARTYGQPLVNFVNASEKNEDSPMETAPEPRGL
RVAIVLSGGQAPGGHNVIAGLFDGLKRWHADSVLIGFLGGPAGVLSGDHIEICADRVDAYRNTGGFDLIGSGRTKIESES
QFAAAAQTVTRMALDALVVVGGDDSNTNAALLAEHFVNSGISTKVIGVPKTIDGDLKNEAIETSFGFDTATKTYSELIGN
IARDACSARKYWHFIKLMGRSASHIALECALKTQPNVCLISEEVAAQSLTLAQIVQSLCDTIATRAQHGEHFGIVLVPEG
LIEFIPEMKALITELNEVMARRAQEFEALDTPDAQRVWIEQALSASARAVFNALPAEISTQLLADRDPHGNVQVSRIDTE
RLLILQVTERLAQMKQEGTYTGVFSSIAHFFGYEGRCAFPSNFDADYCYTLGLTACLLAVHRFTGYVASVRNLTSSVAEW
AVGGVPLTMLMNMERRHGSQKPVIKKALVDLEGMPFRVFSRRRASWALKTSYVYPGAVQYYGPPAVCDEPSVTIRLERPA
PAANSSFGHRSS

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Escherichia coli, GI1790350, Length=242, Percent_Identity=27.6859504132231, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 62428; Mature: 62297

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISLLQQERHRYLPKVPDLLRGDFRRVCARRGLSTTAVADYDALRSLFARTYGQPLVNF
CCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHH
VNASEKNEDSPMETAPEPRGLRVAIVLSGGQAPGGHNVIAGLFDGLKRWHADSVLIGFLG
HCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEECC
GPAGVLSGDHIEICADRVDAYRNTGGFDLIGSGRTKIESESQFAAAAQTVTRMALDALVV
CCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHEEEE
VGGDDSNTNAALLAEHFVNSGISTKVIGVPKTIDGDLKNEAIETSFGFDTATKTYSELIG
ECCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHH
NIARDACSARKYWHFIKLMGRSASHIALECALKTQPNVCLISEEVAAQSLTLAQIVQSLC
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHH
DTIATRAQHGEHFGIVLVPEGLIEFIPEMKALITELNEVMARRAQEFEALDTPDAQRVWI
HHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
EQALSASARAVFNALPAEISTQLLADRDPHGNVQVSRIDTERLLILQVTERLAQMKQEGT
HHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCC
YTGVFSSIAHFFGYEGRCAFPSNFDADYCYTLGLTACLLAVHRFTGYVASVRNLTSSVAE
HHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WAVGGVPLTMLMNMERRHGSQKPVIKKALVDLEGMPFRVFSRRRASWALKTSYVYPGAVQ
HHCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHCEEEEECEECCCCEE
YYGPPAVCDEPSVTIRLERPAPAANSSFGHRSS
ECCCCCCCCCCCEEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
SISLLQQERHRYLPKVPDLLRGDFRRVCARRGLSTTAVADYDALRSLFARTYGQPLVNF
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHH
VNASEKNEDSPMETAPEPRGLRVAIVLSGGQAPGGHNVIAGLFDGLKRWHADSVLIGFLG
HCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEECC
GPAGVLSGDHIEICADRVDAYRNTGGFDLIGSGRTKIESESQFAAAAQTVTRMALDALVV
CCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHEEEE
VGGDDSNTNAALLAEHFVNSGISTKVIGVPKTIDGDLKNEAIETSFGFDTATKTYSELIG
ECCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHH
NIARDACSARKYWHFIKLMGRSASHIALECALKTQPNVCLISEEVAAQSLTLAQIVQSLC
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHH
DTIATRAQHGEHFGIVLVPEGLIEFIPEMKALITELNEVMARRAQEFEALDTPDAQRVWI
HHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
EQALSASARAVFNALPAEISTQLLADRDPHGNVQVSRIDTERLLILQVTERLAQMKQEGT
HHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCC
YTGVFSSIAHFFGYEGRCAFPSNFDADYCYTLGLTACLLAVHRFTGYVASVRNLTSSVAE
HHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WAVGGVPLTMLMNMERRHGSQKPVIKKALVDLEGMPFRVFSRRRASWALKTSYVYPGAVQ
HHCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHCEEEEECEECCCCEE
YYGPPAVCDEPSVTIRLERPAPAANSSFGHRSS
ECCCCCCCCCCCEEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA