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The map label for this gene is ruvA

Identifier: 189025771

GI number: 189025771

Start: 586358

End: 587041

Strand: Reverse

Name: ruvA

Synonym: TPASS_0543

Alternate gene names: 189025771

Gene position: 587041-586358 (Counterclockwise)

Preceding gene: 189025772

Following gene: 189025770

Centisome position: 51.52

GC content: 52.05

Gene sequence:

>684_bases
ATGTTCGAAAGCATCAGCGGTATCTTGACGCTCCATGAGCGCGAGCGCCTCTGTGTTGAGGTGCATGGTATTGAATGGGA
AATTGCAGTGTCCGCATACAGTAGTGCTGCGTTCGGTGAGGTTGGATCACACGTTAAGGTCTTTACCTGGTTGTATCACC
GTGAAGATGCGCTTCGCCTCTTTGGTTTTTCGAATGTACAAGAGCGCACGCTCTTTCTCAGTCTTACAAAGGTAGAAGGT
ATTGGTCCAAAGCAGGCGCTCAAAGTTCTTTCGAGTATTAGCAGTCAGGCTTTGTGTGCAGCGCTTGATACAGGAGATTT
ATGTGCACTCCAGCGCATACCAGGCATTGGAAAAAAGACTGCACAGCGCATGCTCTTAGCGCTGAAAGGTACGCTCGCGC
TAACCGACGCGGCCTCTTGTGCCCAGAGTCAGACAGATGATCGTGCTGCACATCCGAGCAATCTTGGGTGTGCTCCCCAT
GCGAGGGAAATTGAGGATCTGGTAACTGCTCTTGTACAGATGGGATACGACAGGAAAATGGCGGCGGAGGTGATCGCACA
GGAGAGCGCTGCGTTGTGCAGTGTGGGGCGTTCACTGTATGAAGAAGAAGCGACGGTTCTTAAACGTGCTATTCTTGCGT
TGAGTATTGCGCATCCACACGCGGTCGCGCCTGCTGCAGAGTGA

Upstream 100 bases:

>100_bases
AGCCTGCTCGGGAACGCGTTTCTGATCACGATCCGGTCTTTGTGCAATTGTCATGGTAGCGTAGTGTTCGCTTCAGTGCG
CCACACATAAAGAGGAGATA

Downstream 100 bases:

>100_bases
GTTGTGGGGGTGCGGCGGTGCGGTCGTGCTATTTTTCGTATATGAAGGAGTAATCATCGATGAGTATTTCTCTCTTGCAA
CAGGAACGGCATCGCTACCT

Product: Holliday junction DNA helicase, subunit A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MFESISGILTLHERERLCVEVHGIEWEIAVSAYSSAAFGEVGSHVKVFTWLYHREDALRLFGFSNVQERTLFLSLTKVEG
IGPKQALKVLSSISSQALCAALDTGDLCALQRIPGIGKKTAQRMLLALKGTLALTDAASCAQSQTDDRAAHPSNLGCAPH
AREIEDLVTALVQMGYDRKMAAEVIAQESAALCSVGRSLYEEEATVLKRAILALSIAHPHAVAPAAE

Sequences:

>Translated_227_residues
MFESISGILTLHERERLCVEVHGIEWEIAVSAYSSAAFGEVGSHVKVFTWLYHREDALRLFGFSNVQERTLFLSLTKVEG
IGPKQALKVLSSISSQALCAALDTGDLCALQRIPGIGKKTAQRMLLALKGTLALTDAASCAQSQTDDRAAHPSNLGCAPH
AREIEDLVTALVQMGYDRKMAAEVIAQESAALCSVGRSLYEEEATVLKRAILALSIAHPHAVAPAAE
>Mature_227_residues
MFESISGILTLHERERLCVEVHGIEWEIAVSAYSSAAFGEVGSHVKVFTWLYHREDALRLFGFSNVQERTLFLSLTKVEG
IGPKQALKVLSSISSQALCAALDTGDLCALQRIPGIGKKTAQRMLLALKGTLALTDAASCAQSQTDDRAAHPSNLGCAPH
AREIEDLVTALVQMGYDRKMAAEVIAQESAALCSVGRSLYEEEATVLKRAILALSIAHPHAVAPAAE

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG0632

COG function: function code L; Holliday junction resolvasome, DNA-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvA family

Homologues:

Organism=Escherichia coli, GI1788168, Length=201, Percent_Identity=33.3333333333333, Blast_Score=105, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVA_TREPA (O83554)

Other databases:

- EMBL:   AE000520
- PIR:   D71312
- RefSeq:   NP_218982.1
- ProteinModelPortal:   O83554
- SMR:   O83554
- IntAct:   O83554
- GeneID:   2611512
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0543
- NMPDR:   fig|243276.1.peg.541
- TIGR:   TP_0543
- HOGENOM:   HBG635309
- OMA:   LSIETYV
- ProtClustDB:   CLSK218748
- BioCyc:   TPAL243276:TP_0543-MONOMER
- HAMAP:   MF_00031
- InterPro:   IPR013849
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000085
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00278
- TIGRFAMs:   TIGR00084

Pfam domain/function: PF01330 RuvA_N; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =3.6.4.12

Molecular weight: Translated: 24399; Mature: 24399

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFESISGILTLHERERLCVEVHGIEWEIAVSAYSSAAFGEVGSHVKVFTWLYHREDALRL
CCCCHHHHEEHHCCCCEEEEEECCEEEEEEEHHCCHHHHHHCCCCEEHHHHHHHCCHHHH
FGFSNVQERTLFLSLTKVEGIGPKQALKVLSSISSQALCAALDTGDLCALQRIPGIGKKT
HCCCCHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHH
AQRMLLALKGTLALTDAASCAQSQTDDRAAHPSNLGCAPHAREIEDLVTALVQMGYDRKM
HHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHH
AAEVIAQESAALCSVGRSLYEEEATVLKRAILALSIAHPHAVAPAAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MFESISGILTLHERERLCVEVHGIEWEIAVSAYSSAAFGEVGSHVKVFTWLYHREDALRL
CCCCHHHHEEHHCCCCEEEEEECCEEEEEEEHHCCHHHHHHCCCCEEHHHHHHHCCHHHH
FGFSNVQERTLFLSLTKVEGIGPKQALKVLSSISSQALCAALDTGDLCALQRIPGIGKKT
HCCCCHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHH
AQRMLLALKGTLALTDAASCAQSQTDDRAAHPSNLGCAPHAREIEDLVTALVQMGYDRKM
HHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHH
AAEVIAQESAALCSVGRSLYEEEATVLKRAILALSIAHPHAVAPAAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9665876