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The map label for this gene is pgk

Identifier: 189025766

GI number: 189025766

Start: 581387

End: 582646

Strand: Reverse

Name: pgk

Synonym: TPASS_0538

Alternate gene names: 189025766

Gene position: 582646-581387 (Counterclockwise)

Preceding gene: 189025770

Following gene: 189025765

Centisome position: 51.13

GC content: 53.97

Gene sequence:

>1260_bases
ATGATGCTGAGAACTTGTAAAGATGTCACAATGCGAGGTGAGCGTGTTGTCGTGCGTGTTGATTTTAACGTACCGATGCG
TGATGGTATGGTGCAAGACGACACGCGCGTCACTGCAGCAGTTCCTACTCTGCGGTACATCATCGAGCAGGGGCCGCGAC
ACGTAGTGCTCATTAGCCATCTTGGAGACCCTACCCGTGATGCGGATAAAGCAGAGGGGAACGCTAAGAAAGATGGTTGT
CCCTTTGACCGCCACGCATTCATTAACGGCAAACACCGGCTTAAGCCGGTTGCGGACTGCCTTGCAAAGAAGTTGGGGGT
GCCGGTGCATTTTGCGCCTTCCTGCGTCGGGCAGCGTGAATTTATTGAAGGGCTGCCCGACGGTTCGGTAGTGCTTTTGG
AGAATGTGCGCTTTCACCCCGAGGAAACATCTGGTGATGCGAAGGTGCAGGAGCAATTTGCGCGGGAGCTTGCCCAGTAT
GGGGACATTTTTGTTAATGATGCGTTCGGCACTGCGCACCGTGAACATGCATCTACGGTGGTACTCCCGAGACTTATGCG
TCGCAGAGTGGCCGGTCTTTTGATAGAGCGGGAAGTGAGGTACCTGGAACCTATGGTATGCAACCCGAAGGTGCCAATGG
TGGCGGTTGTCGGTGGTGCCAAGGTATCTTCTAAGATAGCAGTCTTGGAGAGTCTGCTTCGAACATCGACGGCGCTTATC
ATCGGGGGAGGGATGGCATACACCTTTTTGAAAGCGCAAGGTGTGGGTGTAGGTACCTCCCTAGTGGAGGATGACTTTAT
CGACACTGCACGTATGCTGTTGCAAAAGGCTCAAAGCGGTGGTGTGTCGGTGGTATTGCCGGTCGATCACGTATGTGCGT
CCACGTTCTGTGCGGATGCGCAGCCGGTTGCGGTGGACGATGTGCATATTCCTATGCACTTGATGGGTATGGATGTTGGT
CCGCGTACGCTTGAGCAGTATCGTGCGCATTTAAAGGGAGTGAGCTCCGTGTTGTGGAATGGTCCGGTAGGGGTTTTTGA
GTTCGATGCCTTTGCACACGGTACACGGGTGCTTGCGCAGTTGATAGCAGAGGCGACGGATGCGGGTGCTACGTCGGTAG
TTGGTGGAGGAGACTCTATTGCAGCAGTCAGCAAGTTTGGGCTTGCAAGTCGCATGAGCCATGTATCTACGGGGGGTGGC
GCATCGCTGAAGCTCTTTGAGGGTAAAGTTTTACCGGGCATTTCGTGTCTTGAAACATAG

Upstream 100 bases:

>100_bases
GCGTCCGACCGATGCGGATATTGAATCTTTTTTTGAAATATAGTGAGAATCAGCCGCAACGTGTGGCGGATGGGGTAACC
CCTGCGTCAAAAAGGAGGAG

Downstream 100 bases:

>100_bases
GATATAGTGGTGCCGTGAGATTTTCCCTTGGGTTGGGGCGAGATCTCCTGGCGAGGGTATGTCTCTGCGGTGGCTCTATA
GAACAGTTTCTCGTGGAGCT

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MMLRTCKDVTMRGERVVVRVDFNVPMRDGMVQDDTRVTAAVPTLRYIIEQGPRHVVLISHLGDPTRDADKAEGNAKKDGC
PFDRHAFINGKHRLKPVADCLAKKLGVPVHFAPSCVGQREFIEGLPDGSVVLLENVRFHPEETSGDAKVQEQFARELAQY
GDIFVNDAFGTAHREHASTVVLPRLMRRRVAGLLIEREVRYLEPMVCNPKVPMVAVVGGAKVSSKIAVLESLLRTSTALI
IGGGMAYTFLKAQGVGVGTSLVEDDFIDTARMLLQKAQSGGVSVVLPVDHVCASTFCADAQPVAVDDVHIPMHLMGMDVG
PRTLEQYRAHLKGVSSVLWNGPVGVFEFDAFAHGTRVLAQLIAEATDAGATSVVGGGDSIAAVSKFGLASRMSHVSTGGG
ASLKLFEGKVLPGISCLET

Sequences:

>Translated_419_residues
MMLRTCKDVTMRGERVVVRVDFNVPMRDGMVQDDTRVTAAVPTLRYIIEQGPRHVVLISHLGDPTRDADKAEGNAKKDGC
PFDRHAFINGKHRLKPVADCLAKKLGVPVHFAPSCVGQREFIEGLPDGSVVLLENVRFHPEETSGDAKVQEQFARELAQY
GDIFVNDAFGTAHREHASTVVLPRLMRRRVAGLLIEREVRYLEPMVCNPKVPMVAVVGGAKVSSKIAVLESLLRTSTALI
IGGGMAYTFLKAQGVGVGTSLVEDDFIDTARMLLQKAQSGGVSVVLPVDHVCASTFCADAQPVAVDDVHIPMHLMGMDVG
PRTLEQYRAHLKGVSSVLWNGPVGVFEFDAFAHGTRVLAQLIAEATDAGATSVVGGGDSIAAVSKFGLASRMSHVSTGGG
ASLKLFEGKVLPGISCLET
>Mature_419_residues
MMLRTCKDVTMRGERVVVRVDFNVPMRDGMVQDDTRVTAAVPTLRYIIEQGPRHVVLISHLGDPTRDADKAEGNAKKDGC
PFDRHAFINGKHRLKPVADCLAKKLGVPVHFAPSCVGQREFIEGLPDGSVVLLENVRFHPEETSGDAKVQEQFARELAQY
GDIFVNDAFGTAHREHASTVVLPRLMRRRVAGLLIEREVRYLEPMVCNPKVPMVAVVGGAKVSSKIAVLESLLRTSTALI
IGGGMAYTFLKAQGVGVGTSLVEDDFIDTARMLLQKAQSGGVSVVLPVDHVCASTFCADAQPVAVDDVHIPMHLMGMDVG
PRTLEQYRAHLKGVSSVLWNGPVGVFEFDAFAHGTRVLAQLIAEATDAGATSVVGGGDSIAAVSKFGLASRMSHVSTGGG
ASLKLFEGKVLPGISCLET

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=431, Percent_Identity=39.907192575406, Blast_Score=310, Evalue=2e-84,
Organism=Homo sapiens, GI31543397, Length=431, Percent_Identity=38.9791183294664, Blast_Score=298, Evalue=8e-81,
Organism=Escherichia coli, GI1789294, Length=422, Percent_Identity=39.0995260663507, Blast_Score=271, Evalue=8e-74,
Organism=Caenorhabditis elegans, GI17508823, Length=425, Percent_Identity=42.5882352941177, Blast_Score=326, Evalue=1e-89,
Organism=Saccharomyces cerevisiae, GI6319857, Length=428, Percent_Identity=39.7196261682243, Blast_Score=311, Evalue=1e-85,
Organism=Drosophila melanogaster, GI24581203, Length=432, Percent_Identity=39.8148148148148, Blast_Score=305, Evalue=3e-83,
Organism=Drosophila melanogaster, GI17136394, Length=432, Percent_Identity=40.2777777777778, Blast_Score=295, Evalue=3e-80,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_TREPA (O83549)

Other databases:

- EMBL:   AE000520
- PIR:   G71311
- RefSeq:   NP_218977.1
- ProteinModelPortal:   O83549
- SMR:   O83549
- IntAct:   O83549
- GeneID:   2611673
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0538
- NMPDR:   fig|243276.1.peg.536
- TIGR:   TP_0538
- HOGENOM:   HBG453500
- OMA:   DCIGDEV
- ProtClustDB:   PRK00073
- BioCyc:   TPAL243276:TP_0538-MONOMER
- BRENDA:   2.7.2.3
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 44944; Mature: 44944

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 36-36 BINDING 137-137 BINDING 174-174 BINDING 225-225 BINDING 316-316 BINDING 347-347

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLRTCKDVTMRGERVVVRVDFNVPMRDGMVQDDTRVTAAVPTLRYIIEQGPRHVVLISH
CCCCCCHHHHCCCCEEEEEEECCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCEEEEEEC
LGDPTRDADKAEGNAKKDGCPFDRHAFINGKHRLKPVADCLAKKLGVPVHFAPSCVGQRE
CCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHCCCEEECCHHCCCHH
FIEGLPDGSVVLLENVRFHPEETSGDAKVQEQFARELAQYGDIFVNDAFGTAHREHASTV
HHHCCCCCCEEEEECCEECCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHH
VLPRLMRRRVAGLLIEREVRYLEPMVCNPKVPMVAVVGGAKVSSKIAVLESLLRTSTALI
HHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCEEEEECCCCHHHHHHHHHHHHHHCCEEE
IGGGMAYTFLKAQGVGVGTSLVEDDFIDTARMLLQKAQSGGVSVVLPVDHVCASTFCADA
EECCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHCCCC
QPVAVDDVHIPMHLMGMDVGPRTLEQYRAHLKGVSSVLWNGPVGVFEFDAFAHGTRVLAQ
CCCEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHH
LIAEATDAGATSVVGGGDSIAAVSKFGLASRMSHVSTGGGASLKLFEGKVLPGISCLET
HHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHCC
>Mature Secondary Structure
MMLRTCKDVTMRGERVVVRVDFNVPMRDGMVQDDTRVTAAVPTLRYIIEQGPRHVVLISH
CCCCCCHHHHCCCCEEEEEEECCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCEEEEEEC
LGDPTRDADKAEGNAKKDGCPFDRHAFINGKHRLKPVADCLAKKLGVPVHFAPSCVGQRE
CCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHCCCEEECCHHCCCHH
FIEGLPDGSVVLLENVRFHPEETSGDAKVQEQFARELAQYGDIFVNDAFGTAHREHASTV
HHHCCCCCCEEEEECCEECCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHH
VLPRLMRRRVAGLLIEREVRYLEPMVCNPKVPMVAVVGGAKVSSKIAVLESLLRTSTALI
HHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCEEEEECCCCHHHHHHHHHHHHHHCCEEE
IGGGMAYTFLKAQGVGVGTSLVEDDFIDTARMLLQKAQSGGVSVVLPVDHVCASTFCADA
EECCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHCCCC
QPVAVDDVHIPMHLMGMDVGPRTLEQYRAHLKGVSSVLWNGPVGVFEFDAFAHGTRVLAQ
CCCEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHH
LIAEATDAGATSVVGGGDSIAAVSKFGLASRMSHVSTGGGASLKLFEGKVLPGISCLET
HHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876