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The map label for this gene is ispDF [H]

Identifier: 189025742

GI number: 189025742

Start: 549056

End: 550303

Strand: Direct

Name: ispDF [H]

Synonym: TPASS_0512

Alternate gene names: 189025742

Gene position: 549056-550303 (Clockwise)

Preceding gene: 189025741

Following gene: 189025743

Centisome position: 48.19

GC content: 57.69

Gene sequence:

>1248_bases
GTGACCCGCGCCGCGTTGCTTGTCACCGCTGCAGGCAGCTCTGCACGCATGCGCAGAGGTGGTGCGTGTGTGCAGAAAAA
GGAGTACTTGCCGTTAACTTCCAGGCAGCCTGGTGTCTGCCTCCTTTCTGAGATACTCGTGCGTGCCCTTGAGGCGCGGT
CGTTCTTCCTTGTCGTTGTTACCGTTCCTGCAGGTGAAGTTGCGTACGCAGAAAGTCAAGTGGCGTGTGATTCGCGTCTT
TCGGCGTTCCCGTCTCGTACGCGTCCAGTCATTCTTTACGTGCCGGGTGCACATACACGCAGTGCGTCTGTGCGCGCGGG
GCTTGACGCGATGGCTACGCATGCGCCCGATGTGGTCCTGGTGCACGACGGCGCACGTCCCTTTGTGAGTGTTGCGCTTA
TTCATTCTGTACTTGAGGCTACCTGTCGCTATGGAGCGGCAGTGCCGGTTATAGAGGCGACGGATACTCCCAAGGGTGTT
GCTGCCGATGGGAGTATCGAAACGCATCTTATACGGAGTCGAGTGCGCTTAGCACAAACCCCTCAGGGTTTTTGCTACGC
GTCGCTGTGCGCTGCCCATCACCGCGCTGCTACTGATGGGGAACAGTATACTGATGACAGCGAGCTGTACGCTCGCTACG
GAGGGACCGTACATGTCTGCGCCGGTGAACGCAGTAATGTAAAGATTACCTATCCGGAGGATCTGGAGCAGCGGGCATCG
GAGCCTGCTCTCACGAGGGGGATATCAGTCCTGCCGTGTACGGAAGAAGGTGCCCTGCGCGTTGGACTGGGGACTGATAT
GCACGCGCTGTGTGCAGGAAGACCGCTGATACTTGCAGGGATACATATTCCCAGTAAGAAGGGAGCGCAGGGTCATTCGG
ATGCAGACGTTCTTGCGCATGCGAGCATTGACGCCCTTTTGGGTGCAGCAGGCCTGGGGGATATTGGCACGTTTTTCCCC
TCTTGTGACGGACGATGGAAAGACGCACATTCTTGCGCGTTGCTCCGCCATACCTGGCAACTGGTGCGCGCAGCGTGCTG
GCGGCTGGTGAATCTCGATGCTGTTGTCTGTCTTGAGCAGCCTGCATTACACCCATTTCGGGAAGCGATGCGCGCATCGC
TCGCGCAGGCCCTGGACACGCACGTAACACGTGTTTTTGTTAAGGCAAAGACTGCCGAACGCCTCGGACCTGTTGGATCT
GGTGCGGCGGTTACCGCACAGGTAGTGGTACTTTTGAAAAAGATATGA

Upstream 100 bases:

>100_bases
CGCTGGGGCTGCGACCCCCTGAGGTTGAAACGCTCATCCATGGCTATTTGGAAAAGGCAAAGGAAAACCCAGTTGTGTCG
GGTACGGTGGATGTGCCCGG

Downstream 100 bases:

>100_bases
GTGGCAGGCGTTGTGCGCCGTGTTCTGCGCGCTGGCCATGCAAAAAAATTTCGTGCTACTATGGCCTTCGGACATGAGGG
TTATTGTTGTTGGATGCGGT

Product: hypothetical protein

Products: NA

Alternate protein names: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MTRAALLVTAAGSSARMRRGGACVQKKEYLPLTSRQPGVCLLSEILVRALEARSFFLVVVTVPAGEVAYAESQVACDSRL
SAFPSRTRPVILYVPGAHTRSASVRAGLDAMATHAPDVVLVHDGARPFVSVALIHSVLEATCRYGAAVPVIEATDTPKGV
AADGSIETHLIRSRVRLAQTPQGFCYASLCAAHHRAATDGEQYTDDSELYARYGGTVHVCAGERSNVKITYPEDLEQRAS
EPALTRGISVLPCTEEGALRVGLGTDMHALCAGRPLILAGIHIPSKKGAQGHSDADVLAHASIDALLGAAGLGDIGTFFP
SCDGRWKDAHSCALLRHTWQLVRAACWRLVNLDAVVCLEQPALHPFREAMRASLAQALDTHVTRVFVKAKTAERLGPVGS
GAAVTAQVVVLLKKI

Sequences:

>Translated_415_residues
MTRAALLVTAAGSSARMRRGGACVQKKEYLPLTSRQPGVCLLSEILVRALEARSFFLVVVTVPAGEVAYAESQVACDSRL
SAFPSRTRPVILYVPGAHTRSASVRAGLDAMATHAPDVVLVHDGARPFVSVALIHSVLEATCRYGAAVPVIEATDTPKGV
AADGSIETHLIRSRVRLAQTPQGFCYASLCAAHHRAATDGEQYTDDSELYARYGGTVHVCAGERSNVKITYPEDLEQRAS
EPALTRGISVLPCTEEGALRVGLGTDMHALCAGRPLILAGIHIPSKKGAQGHSDADVLAHASIDALLGAAGLGDIGTFFP
SCDGRWKDAHSCALLRHTWQLVRAACWRLVNLDAVVCLEQPALHPFREAMRASLAQALDTHVTRVFVKAKTAERLGPVGS
GAAVTAQVVVLLKKI
>Mature_414_residues
TRAALLVTAAGSSARMRRGGACVQKKEYLPLTSRQPGVCLLSEILVRALEARSFFLVVVTVPAGEVAYAESQVACDSRLS
AFPSRTRPVILYVPGAHTRSASVRAGLDAMATHAPDVVLVHDGARPFVSVALIHSVLEATCRYGAAVPVIEATDTPKGVA
ADGSIETHLIRSRVRLAQTPQGFCYASLCAAHHRAATDGEQYTDDSELYARYGGTVHVCAGERSNVKITYPEDLEQRASE
PALTRGISVLPCTEEGALRVGLGTDMHALCAGRPLILAGIHIPSKKGAQGHSDADVLAHASIDALLGAAGLGDIGTFFPS
CDGRWKDAHSCALLRHTWQLVRAACWRLVNLDAVVCLEQPALHPFREAMRASLAQALDTHVTRVFVKAKTAERLGPVGSG
AAVTAQVVVLLKKI

Specific function: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl- D-erythritol 4-phosphate (MEP) (ispD), and converts 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C- methyl-D-erythri

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the ispF family [H]

Homologues:

Organism=Escherichia coli, GI1789103, Length=156, Percent_Identity=42.9487179487179, Blast_Score=116, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294
- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF01128 IspD; PF02542 YgbB [H]

EC number: =2.7.7.60; =4.6.1.12 [H]

Molecular weight: Translated: 44014; Mature: 43883

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: PS01295 ISPD ; PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRAALLVTAAGSSARMRRGGACVQKKEYLPLTSRQPGVCLLSEILVRALEARSFFLVVV
CCCEEEEEEECCCCHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEE
TVPAGEVAYAESQVACDSRLSAFPSRTRPVILYVPGAHTRSASVRAGLDAMATHAPDVVL
ECCCCCEEECCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHCHHHHHCCCCCEEE
VHDGARPFVSVALIHSVLEATCRYGAAVPVIEATDTPKGVAADGSIETHLIRSRVRLAQT
EECCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCC
PQGFCYASLCAAHHRAATDGEQYTDDSELYARYGGTVHVCAGERSNVKITYPEDLEQRAS
CCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCEEEEECCCCCCEEEECCHHHHHHCC
EPALTRGISVLPCTEEGALRVGLGTDMHALCAGRPLILAGIHIPSKKGAQGHSDADVLAH
CCHHHHCCEEEEECCCCCEEEECCCCHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHH
ASIDALLGAAGLGDIGTFFPSCDGRWKDAHSCALLRHTWQLVRAACWRLVNLDAVVCLEQ
HHHHHHHHCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
PALHPFREAMRASLAQALDTHVTRVFVKAKTAERLGPVGSGAAVTAQVVVLLKKI
CCCHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TRAALLVTAAGSSARMRRGGACVQKKEYLPLTSRQPGVCLLSEILVRALEARSFFLVVV
CCEEEEEEECCCCHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEE
TVPAGEVAYAESQVACDSRLSAFPSRTRPVILYVPGAHTRSASVRAGLDAMATHAPDVVL
ECCCCCEEECCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHCHHHHHCCCCCEEE
VHDGARPFVSVALIHSVLEATCRYGAAVPVIEATDTPKGVAADGSIETHLIRSRVRLAQT
EECCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCC
PQGFCYASLCAAHHRAATDGEQYTDDSELYARYGGTVHVCAGERSNVKITYPEDLEQRAS
CCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCEEEEECCCCCCEEEECCHHHHHHCC
EPALTRGISVLPCTEEGALRVGLGTDMHALCAGRPLILAGIHIPSKKGAQGHSDADVLAH
CCHHHHCCEEEEECCCCCEEEECCCCHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHH
ASIDALLGAAGLGDIGTFFPSCDGRWKDAHSCALLRHTWQLVRAACWRLVNLDAVVCLEQ
HHHHHHHHCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
PALHPFREAMRASLAQALDTHVTRVFVKAKTAERLGPVGSGAAVTAQVVVLLKKI
CCCHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]