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The map label for this gene is ppnK

Identifier: 189025673

GI number: 189025673

Start: 468479

End: 469396

Strand: Direct

Name: ppnK

Synonym: TPASS_0441

Alternate gene names: 189025673

Gene position: 468479-469396 (Clockwise)

Preceding gene: 189025672

Following gene: 189025674

Centisome position: 41.11

GC content: 53.81

Gene sequence:

>918_bases
GTGAGCAGCCGCGTGTGTCCTCAGCGGCCTGTTGCAAAATCATCGGGGGACGCGAAGGTGTTGCTTATTGTCAGCACGTA
CAAACCGCGCGCTGCGGTGCTCGCTGCGGACGTTGTGAACTTTCTGAGCATACGTGGATTCCAGTGCCACACCATTGAGT
ATGATGGATTGAATAAAGAAAGCTGTGCTCGCGCAGGCTATATGTTTGCAGTCAGTATTGGGGGGGATGGTACTACACTG
TTTGCCGCGCGCTGTGCTTCTCCTTCTGGTATTCCCATACTTGCCATAAATTTAGGGCGTTTCGGCTTTATCGCTCCTAT
TGAGCCACGGTATTGGCAACAGGCGTTGAGCGATTATTTGGCAGGGGGGGTGCGCCCTGCTGAGCGTGCGCTCATATCGT
GCACCGTCACGCGTGCGGGTAAAGAGATTGCTTCGTGTCTGGCGTTAAACGATGTTGTCCTTTCAAGTGGACGCGTCGCG
CGTCTTACCCGGGCAGAGGTGTGCTTCAACGACATTTCTTTTGGCGTGTATGAAGCTGATGGCATTATTCTTGCGACGCC
TACAGGATCTACTGCGTACTCGGCGGCCTGTGGCGGTCCCATCCTCGATCCGGACCTTGATGCGTTTGTCCTCACTCCCA
TAAGCGCACTGTGCCTTTCTAATCGTCCCGTGGTAGTTCCCTCCTCAGGGGTGGTGCGTATCAAGGTGTTGTCTATGCGA
CACAAAGAAACGGTGCTGTCTGTGGACGGACATGAATTGTGCACGTTGCAGGAAGAAGATCAGCTGCTTGCAAGCAGGTC
ATCGTGCAGCGCACGATTGGTTTTCTGTACACCACACGTGTTCTACCATGCACTGTGCTCGAAACTGGCGTGGTCAGGGA
GTATTTTTTCTCGCAGGGGAAGACGTCACGATGATTGA

Upstream 100 bases:

>100_bases
ATGCATCTGCGCTTTTGTTGCGCTGCGTGCGCTTTCCGTTGTACCCGCGTCTTGCGCCTGCACACGCACAGGAAATTGCG
CGCATCCTTGGGACACTGCC

Downstream 100 bases:

>100_bases
GCAACTTTCGGTGCGCAACGTTGCGCTCATTCAATCTTTGGCGTTGGAGTTTGGTGCACAGTTTACTGCCCTCTCAGGGG
AGACGGGTGCGGGTAAGTCA

Product: hypothetical protein

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MSSRVCPQRPVAKSSGDAKVLLIVSTYKPRAAVLAADVVNFLSIRGFQCHTIEYDGLNKESCARAGYMFAVSIGGDGTTL
FAARCASPSGIPILAINLGRFGFIAPIEPRYWQQALSDYLAGGVRPAERALISCTVTRAGKEIASCLALNDVVLSSGRVA
RLTRAEVCFNDISFGVYEADGIILATPTGSTAYSAACGGPILDPDLDAFVLTPISALCLSNRPVVVPSSGVVRIKVLSMR
HKETVLSVDGHELCTLQEEDQLLASRSSCSARLVFCTPHVFYHALCSKLAWSGSIFSRRGRRHDD

Sequences:

>Translated_305_residues
MSSRVCPQRPVAKSSGDAKVLLIVSTYKPRAAVLAADVVNFLSIRGFQCHTIEYDGLNKESCARAGYMFAVSIGGDGTTL
FAARCASPSGIPILAINLGRFGFIAPIEPRYWQQALSDYLAGGVRPAERALISCTVTRAGKEIASCLALNDVVLSSGRVA
RLTRAEVCFNDISFGVYEADGIILATPTGSTAYSAACGGPILDPDLDAFVLTPISALCLSNRPVVVPSSGVVRIKVLSMR
HKETVLSVDGHELCTLQEEDQLLASRSSCSARLVFCTPHVFYHALCSKLAWSGSIFSRRGRRHDD
>Mature_304_residues
SSRVCPQRPVAKSSGDAKVLLIVSTYKPRAAVLAADVVNFLSIRGFQCHTIEYDGLNKESCARAGYMFAVSIGGDGTTLF
AARCASPSGIPILAINLGRFGFIAPIEPRYWQQALSDYLAGGVRPAERALISCTVTRAGKEIASCLALNDVVLSSGRVAR
LTRAEVCFNDISFGVYEADGIILATPTGSTAYSAACGGPILDPDLDAFVLTPISALCLSNRPVVVPSSGVVRIKVLSMRH
KETVLSVDGHELCTLQEEDQLLASRSSCSARLVFCTPHVFYHALCSKLAWSGSIFSRRGRRHDD

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Homo sapiens, GI55743112, Length=220, Percent_Identity=27.2727272727273, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1788968, Length=277, Percent_Identity=32.4909747292419, Blast_Score=151, Evalue=5e-38,
Organism=Saccharomyces cerevisiae, GI6320794, Length=241, Percent_Identity=28.2157676348548, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6322509, Length=241, Percent_Identity=28.6307053941909, Blast_Score=106, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6325068, Length=208, Percent_Identity=32.6923076923077, Blast_Score=104, Evalue=2e-23,
Organism=Drosophila melanogaster, GI28573830, Length=217, Percent_Identity=30.8755760368664, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28573828, Length=217, Percent_Identity=30.8755760368664, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28573832, Length=217, Percent_Identity=30.8755760368664, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28573826, Length=217, Percent_Identity=30.8755760368664, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI161077047, Length=217, Percent_Identity=30.8755760368664, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI20129957, Length=275, Percent_Identity=25.4545454545455, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24653422, Length=275, Percent_Identity=27.6363636363636, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI281363321, Length=275, Percent_Identity=25.4545454545455, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI281363323, Length=275, Percent_Identity=25.4545454545455, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24653424, Length=275, Percent_Identity=25.4545454545455, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK_TREPA (O83455)

Other databases:

- EMBL:   AE000520
- PIR:   A71324
- RefSeq:   NP_218881.1
- ProteinModelPortal:   O83455
- SMR:   O83455
- IntAct:   O83455
- GeneID:   2610985
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0441
- NMPDR:   fig|243276.1.peg.440
- TIGR:   TP_0441
- HOGENOM:   HBG713904
- OMA:   LNEMVLH
- ProtClustDB:   CLSK218701
- BioCyc:   TPAL243276:TP_0441-MONOMER
- BRENDA:   2.7.1.23
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 32634; Mature: 32503

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
4.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSRVCPQRPVAKSSGDAKVLLIVSTYKPRAAVLAADVVNFLSIRGFQCHTIEYDGLNKE
CCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCHH
SCARAGYMFAVSIGGDGTTLFAARCASPSGIPILAINLGRFGFIAPIEPRYWQQALSDYL
HHHCCCEEEEEEECCCCCEEEEEECCCCCCCEEEEEECCCCEEECCCCHHHHHHHHHHHH
AGGVRPAERALISCTVTRAGKEIASCLALNDVVLSSGRVARLTRAEVCFNDISFGVYEAD
HCCCCHHHHEEEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCEEECC
GIILATPTGSTAYSAACGGPILDPDLDAFVLTPISALCLSNRPVVVPSSGVVRIKVLSMR
CEEEECCCCCCCCHHCCCCCCCCCCCCEEHHHHHHHHHHCCCCEEECCCCEEEEEEEEEC
HKETVLSVDGHELCTLQEEDQLLASRSSCSARLVFCTPHVFYHALCSKLAWSGSIFSRRG
CCCEEEEECCCEEEEECCHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHCCCCHHHHCC
RRHDD
CCCCC
>Mature Secondary Structure 
SSRVCPQRPVAKSSGDAKVLLIVSTYKPRAAVLAADVVNFLSIRGFQCHTIEYDGLNKE
CCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCHH
SCARAGYMFAVSIGGDGTTLFAARCASPSGIPILAINLGRFGFIAPIEPRYWQQALSDYL
HHHCCCEEEEEEECCCCCEEEEEECCCCCCCEEEEEECCCCEEECCCCHHHHHHHHHHHH
AGGVRPAERALISCTVTRAGKEIASCLALNDVVLSSGRVARLTRAEVCFNDISFGVYEAD
HCCCCHHHHEEEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCEEECC
GIILATPTGSTAYSAACGGPILDPDLDAFVLTPISALCLSNRPVVVPSSGVVRIKVLSMR
CEEEECCCCCCCCHHCCCCCCCCCCCCEEHHHHHHHHHHCCCCEEECCCCEEEEEEEEEC
HKETVLSVDGHELCTLQEEDQLLASRSSCSARLVFCTPHVFYHALCSKLAWSGSIFSRRG
CCCEEEEECCCEEEEECCHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHCCCCHHHHCC
RRHDD
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9665876