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The map label for this gene is ispE

Identifier: 189025604

GI number: 189025604

Start: 395959

End: 396834

Strand: Direct

Name: ispE

Synonym: TPASS_0371

Alternate gene names: 189025604

Gene position: 395959-396834 (Clockwise)

Preceding gene: 189025602

Following gene: 189025605

Centisome position: 34.75

GC content: 58.17

Gene sequence:

>876_bases
GTGCAGTCGCTGAGTTTGCGCGCTCACGCAAAGGTGAACATGCATCTGTGGGTTGGTGCACGTCGGGCCGATGGTCTTCA
CTCGATTGAGAGTGTAATGCAGCGCATTACGCTTGCGGATTCTTTGTCTCTTTCGCGCCTGGATATCCCTGGCCGGTGTG
AGGTGTGTTCGCCTTATATGGCTCTGCCTCGAGAAAACACGCTGACCCGTGCGTATGCGCGGTTTTGTCAGGTAACTGGC
GTTCACGATGGTGTGCGCGTGCGCGTAGTGAAGCGTATCCCTGCAGGGTCTGGACTTGGTGGGGGGTCTGCTGATGCTGC
CGCGCTTTTGTGTGGCCTTGATACGTTGTTTGGGACGACCCTTTCTGCTCGGGTTCTACGAGAAGTGGCGTATAGTGTCG
GCAGTGATGTCCCTTTTTTCCTGGCTTCCCAGGCTGCGTGCGTGCTAGGTGGTGGTGAGCAGCTCGTTCCCCTTGTTCCA
AAGACTGGCTATCTGGGGCTTTTGGTCTGGCCGGGATTGCACAGCGGTTCTGCACAGGCCTATGAGGATTTGGACAGGTT
GCGTGCGTGCGGCGTACATGCTGCCGACGGCGAGCAGTACTCTCTGCGTGGGGCTACCGCGCTTTCTGCGCACTATGCAC
AGGATTGTGCGCGGTGGCGGTTTTTTAATAGCTTAGATGCCCCTGTACAGCGTCGGTACCCTGTAGTCGCCCTTGCGCGT
TGGGATCTGGCCCGTGCGGGTGCGTGCTTTACAGCGATGAGTGGAAGCGGGAGCSCGGTTTTTGGTCTGTATCGGGATGA
GGAGGAGCTTCGGCGCGCGCATAAGCTGCTTGCAAAGCGGTGGTGTTGGTGTGTGCGTGTCCGGTTATGTGGGTAG

Upstream 100 bases:

>100_bases
CTGTGCCTAATAGGCACAGGTATTTTTTCTCTTGTGTTTTGGGTGCAGGTAGAGAGAAGGGGGAATTGACACGCTGTGTC
CGCTTGTCATAGAATGGCGG

Downstream 100 bases:

>100_bases
GATATCTTTGGGACGTCGGCAAGTGGTAAGCCAACGGTTTTTGGTACCGTCATTCCGTAGGTTCGAATCCTACCGTCCCA
GTTTGCGGCGTGTTCGCTTT

Product: hypothetical protein

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MQSLSLRAHAKVNMHLWVGARRADGLHSIESVMQRITLADSLSLSRLDIPGRCEVCSPYMALPRENTLTRAYARFCQVTG
VHDGVRVRVVKRIPAGSGLGGGSADAAALLCGLDTLFGTTLSARVLREVAYSVGSDVPFFLASQAACVLGGGEQLVPLVP
KTGYLGLLVWPGLHSGSAQAYEDLDRLRACGVHAADGEQYSLRGATALSAHYAQDCARWRFFNSLDAPVQRRYPVVALAR
WDLARAGACFTAMSGSGSXVFGLYRDEEELRRAHKLLAKRWCWCVRVRLCG

Sequences:

>Translated_291_residues
MQSLSLRAHAKVNMHLWVGARRADGLHSIESVMQRITLADSLSLSRLDIPGRCEVCSPYMALPRENTLTRAYARFCQVTG
VHDGVRVRVVKRIPAGSGLGGGSADAAALLCGLDTLFGTTLSARVLREVAYSVGSDVPFFLASQAACVLGGGEQLVPLVP
KTGYLGLLVWPGLHSGSAQAYEDLDRLRACGVHAADGEQYSLRGATALSAHYAQDCARWRFFNSLDAPVQRRYPVVALAR
WDLARAGACFTAMSGSGSXVFGLYRDEEELRRAHKLLAKRWCWCVRVRLCG
>Mature_291_residues
MQSLSLRAHAKVNMHLWVGARRADGLHSIESVMQRITLADSLSLSRLDIPGRCEVCSPYMALPRENTLTRAYARFCQVTG
VHDGVRVRVVKRIPAGSGLGGGSADAAALLCGLDTLFGTTLSARVLREVAYSVGSDVPFFLASQAACVLGGGEQLVPLVP
KTGYLGLLVWPGLHSGSAQAYEDLDRLRACGVHAADGEQYSLRGATALSAHYAQDCARWRFFNSLDAPVQRRYPVVALAR
WDLARAGACFTAMSGSGSXVFGLYRDEEELRRAHKLLAKRWCWCVRVRLCG

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=273, Percent_Identity=28.9377289377289, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_TREPA (O83386)

Other databases:

- EMBL:   AE000520
- PIR:   B71333
- RefSeq:   NP_218811.1
- ProteinModelPortal:   O83386
- IntAct:   O83386
- GeneID:   2611620
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0371
- NMPDR:   fig|243276.1.peg.370
- TIGR:   TP_0371
- HOGENOM:   HBG734593
- OMA:   DGYHNID
- ProtClustDB:   CLSK218665
- BioCyc:   TPAL243276:TP_0371-MONOMER
- BRENDA:   2.7.1.148
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 31538; Mature: 31538

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 136-136

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSLSLRAHAKVNMHLWVGARRADGLHSIESVMQRITLADSLSLSRLDIPGRCEVCSPYM
CCCCCEEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCHH
ALPRENTLTRAYARFCQVTGVHDGVRVRVVKRIPAGSGLGGGSADAAALLCGLDTLFGTT
HCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHH
LSARVLREVAYSVGSDVPFFLASQAACVLGGGEQLVPLVPKTGYLGLLVWPGLHSGSAQA
HHHHHHHHHHHHHCCCCCHHHHCCCEEEECCCCEEEEECCCCCCEEEEEECCCCCCCHHH
YEDLDRLRACGVHAADGEQYSLRGATALSAHYAQDCARWRFFNSLDAPVQRRYPVVALAR
HHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEEEE
WDLARAGACFTAMSGSGSXVFGLYRDEEELRRAHKLLAKRWCWCVRVRLCG
HHHHHCCCEEEEECCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHEEEECC
>Mature Secondary Structure
MQSLSLRAHAKVNMHLWVGARRADGLHSIESVMQRITLADSLSLSRLDIPGRCEVCSPYM
CCCCCEEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCHH
ALPRENTLTRAYARFCQVTGVHDGVRVRVVKRIPAGSGLGGGSADAAALLCGLDTLFGTT
HCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHH
LSARVLREVAYSVGSDVPFFLASQAACVLGGGEQLVPLVPKTGYLGLLVWPGLHSGSAQA
HHHHHHHHHHHHHCCCCCHHHHCCCEEEECCCCEEEEECCCCCCEEEEEECCCCCCCHHH
YEDLDRLRACGVHAADGEQYSLRGATALSAHYAQDCARWRFFNSLDAPVQRRYPVVALAR
HHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEEEE
WDLARAGACFTAMSGSGSXVFGLYRDEEELRRAHKLLAKRWCWCVRVRLCG
HHHHHCCCEEEEECCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9665876