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The map label for this gene is rep [H]

Identifier: 189025336

GI number: 189025336

Start: 109747

End: 111720

Strand: Reverse

Name: rep [H]

Synonym: TPASS_0102

Alternate gene names: 189025336

Gene position: 111720-109747 (Counterclockwise)

Preceding gene: 189025340

Following gene: 189025333

Centisome position: 9.8

GC content: 45.09

Gene sequence:

>1974_bases
ATGCTCTCTACACTTAATCCTGAGCAACGTCGAGCCGTTACCACACTTGAGGGACCGCTTCTCATTATCGCCGGCGCAGG
CTCAGGGAAAACGCGCGTAATCACGGCGCGCATCGCGTATATGCTCGAATGCGGTATTCTGCAATCGCGCATTCTTGCAC
TGACCTTCACTAACAAGGCCGCACACGAGATGTCTGAACGTATTAAAGCTCTCACGGGCAAACCTTTACGCAACACGACC
GTCAGTACTTTTCACGCTTTTGGCGTTACGATTTTGCGAGAACATATCCATGTTCTCGGCTGGAGGAAGAACTTTAGCAT
TTATGATGAAAATGATAAACGTGCTCTCATTCGCGAAGCAGCAAAGGAAGTACATCTCCTGCCAGAAGTTCTTGACACAA
ACTGCGTGAGCACGCTCTTTTCCGCTATTAAAATGCAGCGTAAGAACTTAAAAGAGCTACAGCACAAGGAACGTGCTCTT
TGGCACGAATATCATACTGCGTTGAAATTATTTAATGCGGTAGACTTTGACGATTTGATCGTACTGCCAATCCAGATCTT
CAGTGAATATCCTGATATCCTTGCTTCATATAAGAGTCGCTATCATTACATTTTAGTGGATGAATTCCAAGACACCAGTG
CACAGCAGTACCGTTTAATGAAAATGCTCGCTACACAAAATATCTGTGTAGTAGGGGACGATGATCAATCAATTTATTCT
TGGCGTGGAGCACACCACGACAATATTCTCTCTTTTGAAAAAGACTTCCCCTACGCCACAGAAATTACCTTAGAGCAAAA
TTACCGTTCCACCGGAACTATTTTGGCTGCCGCAAATGGCGTTATCGCACACAATACACAACGCAAGGAAAAGGCACTGT
GGTCCGGTAACGATTCAGGAAAACCTATTGAAATCTTCAATCCCGAAACGGAACGTGATGAAGCAATCTTTATTGCAAAC
ACGATTCTCGCAGAACAAATACGCGCATCCTATTCTTTCAGTAGTTTTGGAGTCCTACTCCGCACGAATAGTTTTATGCG
CATAATTGAAGACGTATTTTTGCAAGAAAATATTCCATACCGTGTCTCAGGCGGCATGAGCTTTTTTCAGAGGAAAGAAA
TAAAGGATGTGCTCAGTTATCTGCGGGTAATTTCTAATCCTGACGACGATGTCAACCTATTACGCATCATTAATACCCCG
AGGCGAGGAATTGGAAAAAAAACACTGCATCTAGTCTCCGACATTGCTAATACACAACAATGTTCCGTGTTCAATGCACT
GAATCAAATTATCAATAAAACACATGCAGTCGATTTAAAAGAATCACATCGTACCGCTGTGGAAAATTTTCTCCAGCTCA
TTACCCAGGCGCGTACCCATCTACTTTCGGGGAAAAATCTTGCGTACAAGGTACGCAAATTTGTTGAAGACATTCAGTAT
TTTAATTACCTCATCCAAGAATTCCAAAAAAACGAACATGCCGCACGTTTTAAATTCTTGCAAATCGAACACTTAGTCGA
ATCCATCGAACACTGGGAACAGAGTTCAGAACACGGAAGCTTGTACGACTACCTAAACCGCGTTACGTTGCTAGCACGCG
ATAACGTCCAAAAAGAAACAGAGGGCGCTGTGTCGCTCATGACAATTCATGCGTCGAAGGGGCTGGAATTTCCCGTAGTG
TTTATCGCCGGCGTGGAAGCGGGAACCATTCCACACGAACGAAGTATAGAAGAGGTTCACAGCATCGAGGAAGAACGACG
CCTTTTCTACGTTGCAATTACCCGCGCAAAAGAGAAGTTGTACCTGACACACTGCCGCACAAGGAAACGCGGCGGAAAAA
GGGAAGAGCGCACCGCCTCCCCCTTTCTGGACGAAATTCCCAAACACCTGGTGCGCGCCCATGCACCGGAAGGAAAAATA
AGCGAGGAAGAGGTCACGCGGGCCTTCACACGCATCAAAAAACTATTTACGTGA

Upstream 100 bases:

>100_bases
TCCATCCATCTCTACCCAATCTGAAGTATTACCCACAGTCTATGCCCTTCCACATACGGATATTCTTTTTTCCGCCCTAT
TTTGCTACGCTTGTGCGTCT

Downstream 100 bases:

>100_bases
TGGCTACCCACCCGCACAGCCCCAAAGCCGCTCGCGCTCAAACCCCCTGGTACAAAAACCACTGCGCAAGAAGTGCCGCT
ATTTGCCGAAGAGGGGTTAT

Product: Rep helicase, single-stranded DNA-dependent ATPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 657; Mature: 657

Protein sequence:

>657_residues
MLSTLNPEQRRAVTTLEGPLLIIAGAGSGKTRVITARIAYMLECGILQSRILALTFTNKAAHEMSERIKALTGKPLRNTT
VSTFHAFGVTILREHIHVLGWRKNFSIYDENDKRALIREAAKEVHLLPEVLDTNCVSTLFSAIKMQRKNLKELQHKERAL
WHEYHTALKLFNAVDFDDLIVLPIQIFSEYPDILASYKSRYHYILVDEFQDTSAQQYRLMKMLATQNICVVGDDDQSIYS
WRGAHHDNILSFEKDFPYATEITLEQNYRSTGTILAAANGVIAHNTQRKEKALWSGNDSGKPIEIFNPETERDEAIFIAN
TILAEQIRASYSFSSFGVLLRTNSFMRIIEDVFLQENIPYRVSGGMSFFQRKEIKDVLSYLRVISNPDDDVNLLRIINTP
RRGIGKKTLHLVSDIANTQQCSVFNALNQIINKTHAVDLKESHRTAVENFLQLITQARTHLLSGKNLAYKVRKFVEDIQY
FNYLIQEFQKNEHAARFKFLQIEHLVESIEHWEQSSEHGSLYDYLNRVTLLARDNVQKETEGAVSLMTIHASKGLEFPVV
FIAGVEAGTIPHERSIEEVHSIEEERRLFYVAITRAKEKLYLTHCRTRKRGGKREERTASPFLDEIPKHLVRAHAPEGKI
SEEEVTRAFTRIKKLFT

Sequences:

>Translated_657_residues
MLSTLNPEQRRAVTTLEGPLLIIAGAGSGKTRVITARIAYMLECGILQSRILALTFTNKAAHEMSERIKALTGKPLRNTT
VSTFHAFGVTILREHIHVLGWRKNFSIYDENDKRALIREAAKEVHLLPEVLDTNCVSTLFSAIKMQRKNLKELQHKERAL
WHEYHTALKLFNAVDFDDLIVLPIQIFSEYPDILASYKSRYHYILVDEFQDTSAQQYRLMKMLATQNICVVGDDDQSIYS
WRGAHHDNILSFEKDFPYATEITLEQNYRSTGTILAAANGVIAHNTQRKEKALWSGNDSGKPIEIFNPETERDEAIFIAN
TILAEQIRASYSFSSFGVLLRTNSFMRIIEDVFLQENIPYRVSGGMSFFQRKEIKDVLSYLRVISNPDDDVNLLRIINTP
RRGIGKKTLHLVSDIANTQQCSVFNALNQIINKTHAVDLKESHRTAVENFLQLITQARTHLLSGKNLAYKVRKFVEDIQY
FNYLIQEFQKNEHAARFKFLQIEHLVESIEHWEQSSEHGSLYDYLNRVTLLARDNVQKETEGAVSLMTIHASKGLEFPVV
FIAGVEAGTIPHERSIEEVHSIEEERRLFYVAITRAKEKLYLTHCRTRKRGGKREERTASPFLDEIPKHLVRAHAPEGKI
SEEEVTRAFTRIKKLFT
>Mature_657_residues
MLSTLNPEQRRAVTTLEGPLLIIAGAGSGKTRVITARIAYMLECGILQSRILALTFTNKAAHEMSERIKALTGKPLRNTT
VSTFHAFGVTILREHIHVLGWRKNFSIYDENDKRALIREAAKEVHLLPEVLDTNCVSTLFSAIKMQRKNLKELQHKERAL
WHEYHTALKLFNAVDFDDLIVLPIQIFSEYPDILASYKSRYHYILVDEFQDTSAQQYRLMKMLATQNICVVGDDDQSIYS
WRGAHHDNILSFEKDFPYATEITLEQNYRSTGTILAAANGVIAHNTQRKEKALWSGNDSGKPIEIFNPETERDEAIFIAN
TILAEQIRASYSFSSFGVLLRTNSFMRIIEDVFLQENIPYRVSGGMSFFQRKEIKDVLSYLRVISNPDDDVNLLRIINTP
RRGIGKKTLHLVSDIANTQQCSVFNALNQIINKTHAVDLKESHRTAVENFLQLITQARTHLLSGKNLAYKVRKFVEDIQY
FNYLIQEFQKNEHAARFKFLQIEHLVESIEHWEQSSEHGSLYDYLNRVTLLARDNVQKETEGAVSLMTIHASKGLEFPVV
FIAGVEAGTIPHERSIEEVHSIEEERRLFYVAITRAKEKLYLTHCRTRKRGGKREERTASPFLDEIPKHLVRAHAPEGKI
SEEEVTRAFTRIKKLFT

Specific function: DNA helicase. Has a broad nucleotide specificity, even being able to hydrolyze ethenonucleotides, and is able to couple the hydrolysis to unwinding of DNA substrates. It is a 3'-5' helicase but at high protein concentrations it can also displace a substra

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=647, Percent_Identity=38.4853168469861, Blast_Score=393, Evalue=1e-110,
Organism=Escherichia coli, GI2367296, Length=644, Percent_Identity=36.6459627329193, Blast_Score=387, Evalue=1e-109,
Organism=Escherichia coli, GI1787196, Length=342, Percent_Identity=30.7017543859649, Blast_Score=112, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6322369, Length=785, Percent_Identity=27.2611464968153, Blast_Score=209, Evalue=1e-54,
Organism=Saccharomyces cerevisiae, GI6324477, Length=452, Percent_Identity=23.0088495575221, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 75463; Mature: 75463

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSTLNPEQRRAVTTLEGPLLIIAGAGSGKTRVITARIAYMLECGILQSRILALTFTNKA
CCCCCCHHHHHHHEEECCCEEEEEECCCCCEEEHHHHHHHHHHHHHHHHCEEEEEECCHH
AHEMSERIKALTGKPLRNTTVSTFHAFGVTILREHIHVLGWRKNFSIYDENDKRALIREA
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCEECCCCHHHHHHHH
AKEVHLLPEVLDTNCVSTLFSAIKMQRKNLKELQHKERALWHEYHTALKLFNAVDFDDLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
VLPIQIFSEYPDILASYKSRYHYILVDEFQDTSAQQYRLMKMLATQNICVVGDDDQSIYS
EHHHHHHHHCHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHCCEEEECCCCCHHHH
WRGAHHDNILSFEKDFPYATEITLEQNYRSTGTILAAANGVIAHNTQRKEKALWSGNDSG
HCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEECCCEEECCCCHHHHHHHCCCCCC
KPIEIFNPETERDEAIFIANTILAEQIRASYSFSSFGVLLRTNSFMRIIEDVFLQENIPY
CCEEEECCCCCCCCEEEEHHHHHHHHHHHHCCHHHCEEEEECCHHHHHHHHHHHCCCCCE
RVSGGMSFFQRKEIKDVLSYLRVISNPDDDVNLLRIINTPRRGIGKKTLHLVSDIANTQQ
EECCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCHH
CSVFNALNQIINKTHAVDLKESHRTAVENFLQLITQARTHLLSGKNLAYKVRKFVEDIQY
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
FNYLIQEFQKNEHAARFKFLQIEHLVESIEHWEQSSEHGSLYDYLNRVTLLARDNVQKET
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEECCCCCHHH
EGAVSLMTIHASKGLEFPVVFIAGVEAGTIPHERSIEEVHSIEEERRLFYVAITRAKEKL
CCCEEEEEEECCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCEEEEEEEEHHHHHH
YLTHCRTRKRGGKREERTASPFLDEIPKHLVRAHAPEGKISEEEVTRAFTRIKKLFT
HHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLSTLNPEQRRAVTTLEGPLLIIAGAGSGKTRVITARIAYMLECGILQSRILALTFTNKA
CCCCCCHHHHHHHEEECCCEEEEEECCCCCEEEHHHHHHHHHHHHHHHHCEEEEEECCHH
AHEMSERIKALTGKPLRNTTVSTFHAFGVTILREHIHVLGWRKNFSIYDENDKRALIREA
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCEECCCCHHHHHHHH
AKEVHLLPEVLDTNCVSTLFSAIKMQRKNLKELQHKERALWHEYHTALKLFNAVDFDDLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
VLPIQIFSEYPDILASYKSRYHYILVDEFQDTSAQQYRLMKMLATQNICVVGDDDQSIYS
EHHHHHHHHCHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHCCEEEECCCCCHHHH
WRGAHHDNILSFEKDFPYATEITLEQNYRSTGTILAAANGVIAHNTQRKEKALWSGNDSG
HCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEECCCEEECCCCHHHHHHHCCCCCC
KPIEIFNPETERDEAIFIANTILAEQIRASYSFSSFGVLLRTNSFMRIIEDVFLQENIPY
CCEEEECCCCCCCCEEEEHHHHHHHHHHHHCCHHHCEEEEECCHHHHHHHHHHHCCCCCE
RVSGGMSFFQRKEIKDVLSYLRVISNPDDDVNLLRIINTPRRGIGKKTLHLVSDIANTQQ
EECCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCHH
CSVFNALNQIINKTHAVDLKESHRTAVENFLQLITQARTHLLSGKNLAYKVRKFVEDIQY
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
FNYLIQEFQKNEHAARFKFLQIEHLVESIEHWEQSSEHGSLYDYLNRVTLLARDNVQKET
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEECCCCCHHH
EGAVSLMTIHASKGLEFPVVFIAGVEAGTIPHERSIEEVHSIEEERRLFYVAITRAKEKL
CCCEEEEEEECCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCEEEEEEEEHHHHHH
YLTHCRTRKRGGKREERTASPFLDEIPKHLVRAHAPEGKISEEEVTRAFTRIKKLFT
HHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9592155; 8934527; 10199404; 10388562; 10454638 [H]