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The map label for this gene is prfA [H]

Identifier: 189025285

GI number: 189025285

Start: 59575

End: 60630

Strand: Direct

Name: prfA [H]

Synonym: TPASS_0051

Alternate gene names: 189025285

Gene position: 59575-60630 (Clockwise)

Preceding gene: 189025278

Following gene: 189025286

Centisome position: 5.23

GC content: 49.81

Gene sequence:

>1056_bases
GTGATAGAAAAGTTGGAAGAACTGCGCGCTCAGTGGAGAAAACTACAGCAGGAAGTGGAGAATCCTTCGCTTTTCTCTTC
CACTCAGAGTTATCGTGAACGTATGCGCGATCACGCCTATCTTTCCAGACTGATGGAAGAGTATGATCGCTATTTGCTTA
CTGAGAAGCAGTTGGAAGACGCGCACGTTCTCATCCAAGATGAGTCGGATGCTGATTTTAAGGACGTTATTCGGCAAGAG
ATCCGTACACTTGAAGCTGCACTGCACACGAGTCAAAAGCGACTAAAGACGCTGCTTATTCCCCCCGACCCTTTGCAAGA
GAAGAATATTATCATGGAAATTCGCGGCGGTACCGGCGGTGATGAAGCAGCGCTCTTTGCTGCAGATCTATTTAGAATGT
ACACGCACTACGCTGAGTCAAAACAATGGCGCTATGAAGTCCTTGCAGTGAGCGAAACAGAGTTGGGAGGATTTAAGGAA
ATTACGTTCTCTATCTCGGGGCGCGATGTGTATGGCAGTTTACGTTATGAATCGGGTGTGCATCGCGTTCAACGTGTCCC
TAGCACTGAAGCGTCGGGGCGCATCCATACCAGTGCGGTTACCGTTGCAGTGCTGCCTGAGATGGAAGAGACTGAAGTGG
ACATTCGTGCTGAGGACGTGCGTGTTGATGTCATGCGTGCAAGTGGTCCTGGTGGGCAGTGTGTCAACACCACTGATTCT
GCGGTGCGTCTTACACATCTACCTACGGGCATTGTCGTTGTCTGTCAGGACGAGAAGAGTCAAATCAAAAACAAAGCCAA
GGCCATGCGTGTATTGCGCAGCAGAGTGTATGATTTAGAGGAATCGAAGCGCCAGGTTGCCCGTGCAAGGGAACGCAAAA
GTCAAGTTGGTTCAGGGGATCGTTCCGAGCGCATTCGCACGTATAATTTTCCTCAGAACCGTGTTACGGATCATCGCGTG
CGTGTTACGCTCTACAAGCTAGATGCAGTGATGCAGGGTGCGTTGGATGACATTATCGAGCCATTGTGTATTGCGTCTCG
AGAGAGTGTAATCTAG

Upstream 100 bases:

>100_bases
TAGCATCATGGCAAGCTCTTTTTCTATTCGTGTCGTGGCCTGGAACTGTCTTTGTTGAAAGCTTCGCCTGAATATTTTAT
GCTCCTGCGCGAGGGCCCCC

Downstream 100 bases:

>100_bases
TGCAAGAACTCTGTACGATTCGACAGGCGCGTATGTACGCGCGAGCGTTGTTTCAAGACGCCCCCTGTTTGCGCGGACAG
AACACACCGCTTTTAGATGC

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MIEKLEELRAQWRKLQQEVENPSLFSSTQSYRERMRDHAYLSRLMEEYDRYLLTEKQLEDAHVLIQDESDADFKDVIRQE
IRTLEAALHTSQKRLKTLLIPPDPLQEKNIIMEIRGGTGGDEAALFAADLFRMYTHYAESKQWRYEVLAVSETELGGFKE
ITFSISGRDVYGSLRYESGVHRVQRVPSTEASGRIHTSAVTVAVLPEMEETEVDIRAEDVRVDVMRASGPGGQCVNTTDS
AVRLTHLPTGIVVVCQDEKSQIKNKAKAMRVLRSRVYDLEESKRQVARARERKSQVGSGDRSERIRTYNFPQNRVTDHRV
RVTLYKLDAVMQGALDDIIEPLCIASRESVI

Sequences:

>Translated_351_residues
MIEKLEELRAQWRKLQQEVENPSLFSSTQSYRERMRDHAYLSRLMEEYDRYLLTEKQLEDAHVLIQDESDADFKDVIRQE
IRTLEAALHTSQKRLKTLLIPPDPLQEKNIIMEIRGGTGGDEAALFAADLFRMYTHYAESKQWRYEVLAVSETELGGFKE
ITFSISGRDVYGSLRYESGVHRVQRVPSTEASGRIHTSAVTVAVLPEMEETEVDIRAEDVRVDVMRASGPGGQCVNTTDS
AVRLTHLPTGIVVVCQDEKSQIKNKAKAMRVLRSRVYDLEESKRQVARARERKSQVGSGDRSERIRTYNFPQNRVTDHRV
RVTLYKLDAVMQGALDDIIEPLCIASRESVI
>Mature_351_residues
MIEKLEELRAQWRKLQQEVENPSLFSSTQSYRERMRDHAYLSRLMEEYDRYLLTEKQLEDAHVLIQDESDADFKDVIRQE
IRTLEAALHTSQKRLKTLLIPPDPLQEKNIIMEIRGGTGGDEAALFAADLFRMYTHYAESKQWRYEVLAVSETELGGFKE
ITFSISGRDVYGSLRYESGVHRVQRVPSTEASGRIHTSAVTVAVLPEMEETEVDIRAEDVRVDVMRASGPGGQCVNTTDS
AVRLTHLPTGIVVVCQDEKSQIKNKAKAMRVLRSRVYDLEESKRQVARARERKSQVGSGDRSERIRTYNFPQNRVTDHRV
RVTLYKLDAVMQGALDDIIEPLCIASRESVI

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=290, Percent_Identity=45.8620689655172, Blast_Score=274, Evalue=7e-74,
Organism=Homo sapiens, GI34577120, Length=359, Percent_Identity=37.883008356546, Blast_Score=232, Evalue=4e-61,
Organism=Homo sapiens, GI166795305, Length=195, Percent_Identity=43.5897435897436, Blast_Score=178, Evalue=6e-45,
Organism=Escherichia coli, GI1787462, Length=343, Percent_Identity=50.1457725947522, Blast_Score=342, Evalue=2e-95,
Organism=Escherichia coli, GI2367172, Length=341, Percent_Identity=34.6041055718475, Blast_Score=193, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI17542784, Length=246, Percent_Identity=39.8373983739837, Blast_Score=182, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6321295, Length=320, Percent_Identity=43.75, Blast_Score=251, Evalue=2e-67,
Organism=Drosophila melanogaster, GI19921226, Length=287, Percent_Identity=42.5087108013937, Blast_Score=238, Evalue=6e-63,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40205; Mature: 40205

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEKLEELRAQWRKLQQEVENPSLFSSTQSYRERMRDHAYLSRLMEEYDRYLLTEKQLED
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
AHVLIQDESDADFKDVIRQEIRTLEAALHTSQKRLKTLLIPPDPLQEKNIIMEIRGGTGG
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEECCCCCC
DEAALFAADLFRMYTHYAESKQWRYEVLAVSETELGGFKEITFSISGRDVYGSLRYESGV
CHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCEEEEEEEECCCEEEEECHHHHHH
HRVQRVPSTEASGRIHTSAVTVAVLPEMEETEVDIRAEDVRVDVMRASGPGGQCVNTTDS
HHHHHCCCCCCCCEEEEEEEEEEECCCCCCCCCCEEECCEEEEEEEECCCCCCEECCCCC
AVRLTHLPTGIVVVCQDEKSQIKNKAKAMRVLRSRVYDLEESKRQVARARERKSQVGSGD
EEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RSERIRTYNFPQNRVTDHRVRVTLYKLDAVMQGALDDIIEPLCIASRESVI
CCCCCEEECCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIEKLEELRAQWRKLQQEVENPSLFSSTQSYRERMRDHAYLSRLMEEYDRYLLTEKQLED
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
AHVLIQDESDADFKDVIRQEIRTLEAALHTSQKRLKTLLIPPDPLQEKNIIMEIRGGTGG
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEECCCCCC
DEAALFAADLFRMYTHYAESKQWRYEVLAVSETELGGFKEITFSISGRDVYGSLRYESGV
CHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCEEEEEEEECCCEEEEECHHHHHH
HRVQRVPSTEASGRIHTSAVTVAVLPEMEETEVDIRAEDVRVDVMRASGPGGQCVNTTDS
HHHHHCCCCCCCCEEEEEEEEEEECCCCCCCCCCEEECCEEEEEEEECCCCCCEECCCCC
AVRLTHLPTGIVVVCQDEKSQIKNKAKAMRVLRSRVYDLEESKRQVARARERKSQVGSGD
EEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RSERIRTYNFPQNRVTDHRVRVTLYKLDAVMQGALDDIIEPLCIASRESVI
CCCCCEEECCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]