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The map label for this gene is hemK [H]

Identifier: 189025286

GI number: 189025286

Start: 60600

End: 61646

Strand: Direct

Name: hemK [H]

Synonym: TPASS_0052

Alternate gene names: 189025286

Gene position: 60600-61646 (Clockwise)

Preceding gene: 189025285

Following gene: 189025288

Centisome position: 5.32

GC content: 55.21

Gene sequence:

>1047_bases
GTGTATTGCGTCTCGAGAGAGTGTAATCTAGTGCAAGAACTCTGTACGATTCGACAGGCGCGTATGTACGCGCGAGCGTT
GTTTCAAGACGCCCCCTGTTTGCGCGGACAGAACACACCGCTTTTAGATGCAGACCTTATTCTGTCGAAGTTGCTTGCGA
AGCCGCGTGCGTGGATTCTCGCCCACCAGCAGGATGAGATTGCCTCCGTTGCACACGAGTTTAAGCGTCTCGTGCATCTT
CGTTGTAGGGGACGTGCGTTGGCGTATCTGACTCGAGAAAAAGAGTTTTTTGGTCTGAGATTCCGTGTCACCCGTGCTAC
GCTTATCCCTAAACCGGATACCGAATTGCTTGTAGAAAGTGTCCTGGCGCACGTTGCGTCCCAAATGATGAAGCCGCGTT
CAGTATCTGTGCATAAAGACACAAGTGCACTGCCTGTCTTGAAGATATTCGAGGCGTGTACGGGATGCGGGTGTATTGCC
ATTGCACTTATGCATATGTTGCGTGCGCGTGGCACGCCACCTCTCTATGTCATTGCATCCGACATTTGCATGCGGGCCCT
TGCCGTAGCGCGGTATAACGCGCGCCGACTCTTGGATGTATCTGCAAATTCGCGCGTACGTTTCGTGCACGCAGATGTGC
GTGCTCCTATTCCGTTCTTTTCTCCTTCTGAAGGCACGGACGTGGTACAGGAGCGCGGGGTGTGCGTTCCGTATGATGTG
ATATGTGCAAATCCGCCTTACGTACCGAGTGCGCAAGCGCGCGCGCTGTTGCAGGACGGGAGAGGGGAGCCTCTCGGTGC
CTTAGATGGGGGTGCAGATGGGCTAGACTTGGTTCGCGCATTCGCACACCACAGTGCCGCAGCGCTAAAGGAAGGCGGGT
GCGTGTTTTGCGAGGTCGGCTCAAACCACGCACAACGTGCAGCGCGCATCTTCCAGGCAGCAGGGTTTGCCACGGTGAAA
ATTTCAAAAGATCTCTCCGGGAAAGAGCGCCTGATTAGCGGGATACTGCGCTCGCAGTCTAGAGCTGTAACAGCGCCGAG
TGGCTAG

Upstream 100 bases:

>100_bases
ATTTTCCTCAGAACCGTGTTACGGATCATCGCGTGCGTGTTACGCTCTACAAGCTAGATGCAGTGATGCAGGGTGCGTTG
GATGACATTATCGAGCCATT

Downstream 100 bases:

>100_bases
GGTGAAACACGGCGACTGAGTGGTTATCCTGGCGTTTGCAGGTGGATGTCCGCGCCGCGTTGGCCGATAGGCTGAGTACA
TGAAGGAGTTAGAGATCATC

Product: protoporphyrinogen oxidase

Products: NA

Alternate protein names: HemK protein homolog; tRNA (guanine-N(7)-)-methyltransferase; tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MYCVSRECNLVQELCTIRQARMYARALFQDAPCLRGQNTPLLDADLILSKLLAKPRAWILAHQQDEIASVAHEFKRLVHL
RCRGRALAYLTREKEFFGLRFRVTRATLIPKPDTELLVESVLAHVASQMMKPRSVSVHKDTSALPVLKIFEACTGCGCIA
IALMHMLRARGTPPLYVIASDICMRALAVARYNARRLLDVSANSRVRFVHADVRAPIPFFSPSEGTDVVQERGVCVPYDV
ICANPPYVPSAQARALLQDGRGEPLGALDGGADGLDLVRAFAHHSAAALKEGGCVFCEVGSNHAQRAARIFQAAGFATVK
ISKDLSGKERLISGILRSQSRAVTAPSG

Sequences:

>Translated_348_residues
MYCVSRECNLVQELCTIRQARMYARALFQDAPCLRGQNTPLLDADLILSKLLAKPRAWILAHQQDEIASVAHEFKRLVHL
RCRGRALAYLTREKEFFGLRFRVTRATLIPKPDTELLVESVLAHVASQMMKPRSVSVHKDTSALPVLKIFEACTGCGCIA
IALMHMLRARGTPPLYVIASDICMRALAVARYNARRLLDVSANSRVRFVHADVRAPIPFFSPSEGTDVVQERGVCVPYDV
ICANPPYVPSAQARALLQDGRGEPLGALDGGADGLDLVRAFAHHSAAALKEGGCVFCEVGSNHAQRAARIFQAAGFATVK
ISKDLSGKERLISGILRSQSRAVTAPSG
>Mature_348_residues
MYCVSRECNLVQELCTIRQARMYARALFQDAPCLRGQNTPLLDADLILSKLLAKPRAWILAHQQDEIASVAHEFKRLVHL
RCRGRALAYLTREKEFFGLRFRVTRATLIPKPDTELLVESVLAHVASQMMKPRSVSVHKDTSALPVLKIFEACTGCGCIA
IALMHMLRARGTPPLYVIASDICMRALAVARYNARRLLDVSANSRVRFVHADVRAPIPFFSPSEGTDVVQERGVCVPYDV
ICANPPYVPSAQARALLQDGRGEPLGALDGGADGLDLVRAFAHHSAAALKEGGCVFCEVGSNHAQRAARIFQAAGFATVK
ISKDLSGKERLISGILRSQSRAVTAPSG

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=263, Percent_Identity=28.8973384030418, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1787463, Length=313, Percent_Identity=27.7955271565495, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI87082085, Length=216, Percent_Identity=31.4814814814815, Blast_Score=78, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848
- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4; PF05175 MTS [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 38014; Mature: 38014

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYCVSRECNLVQELCTIRQARMYARALFQDAPCLRGQNTPLLDADLILSKLLAKPRAWIL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEE
AHQQDEIASVAHEFKRLVHLRCRGRALAYLTREKEFFGLRFRVTRATLIPKPDTELLVES
ECCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHEEEEEEEEEEECCCCCHHHHHHH
VLAHVASQMMKPRSVSVHKDTSALPVLKIFEACTGCGCIAIALMHMLRARGTPPLYVIAS
HHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEHHH
DICMRALAVARYNARRLLDVSANSRVRFVHADVRAPIPFFSPSEGTDVVQERGVCVPYDV
HHHHHHHHHHHHCCHHEEEECCCCEEEEEEECCCCCCCCCCCCCCCHHHHHCCCCCCCCE
ICANPPYVPSAQARALLQDGRGEPLGALDGGADGLDLVRAFAHHSAAALKEGGCVFCEVG
EECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEC
SNHAQRAARIFQAAGFATVKISKDLSGKERLISGILRSQSRAVTAPSG
CHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MYCVSRECNLVQELCTIRQARMYARALFQDAPCLRGQNTPLLDADLILSKLLAKPRAWIL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEE
AHQQDEIASVAHEFKRLVHLRCRGRALAYLTREKEFFGLRFRVTRATLIPKPDTELLVES
ECCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHEEEEEEEEEEECCCCCHHHHHHH
VLAHVASQMMKPRSVSVHKDTSALPVLKIFEACTGCGCIAIALMHMLRARGTPPLYVIAS
HHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEHHH
DICMRALAVARYNARRLLDVSANSRVRFVHADVRAPIPFFSPSEGTDVVQERGVCVPYDV
HHHHHHHHHHHHCCHHEEEECCCCEEEEEEECCCCCCCCCCCCCCCHHHHHCCCCCCCCE
ICANPPYVPSAQARALLQDGRGEPLGALDGGADGLDLVRAFAHHSAAALKEGGCVFCEVG
EECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEC
SNHAQRAARIFQAAGFATVKISKDLSGKERLISGILRSQSRAVTAPSG
CHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA