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The map label for this gene is gidA

Identifier: 189025278

GI number: 189025278

Start: 52587

End: 54479

Strand: Direct

Name: gidA

Synonym: TPASS_0044

Alternate gene names: 189025278

Gene position: 52587-54479 (Clockwise)

Preceding gene: 189025277

Following gene: 189025285

Centisome position: 4.62

GC content: 49.34

Gene sequence:

>1893_bases
ATGGGTTTCAGATTTTCTGACTATGACGTTATCGTCGTCGGTGGTGGACACGCAGGTGCCGAAGCGGCGCTGGCCGCTGC
CCGTATGGGGGAGCACACGTTACTCATCACTCAGACAATCGATAGTATCGGCAGGCTCTCATGTAATCCTTCCATTGGAG
GAATTTCCAAGGGGAATATTGTAAGAGAAATCGATGCACTCGGCGGAGAGATGGGAAAGTTTGCGGATGCATGCATGATT
CAGTATCGACTGCTCAACAAAAGCCGGGGCCCTGCAGTGCAGGCGCCGCGTATCCAAGCAGATAAGTTTTTGTATGCCCA
GAAGGTGAAGTATACGTTGGAATGTACGCAGCATCTTCACCTGTATCAGGACACAGTGGTAGACGTTGTGTGTTCCAATA
CCACTGATGCAGGATATGTAGCGTATGGGGCAGCGCATGCGGTAGTTACCGCACGCGGCAGACGCATCTCTGCACGTGCG
GTGGTACTGACTACGGGGACCTTTATGGAAGGGCGTGTGTATATTGGGGAATATGAGGCACCTGAAGGCCGATTAGGGGA
ACATGCCGCTGAAGGGCTGGGAGCTGCGCTGAGAAAAAAGGGGTTTCAGATGGGACGGCTGAAGACGGGTACGCCCGCGC
GCGTACTACGAAAATCTGTAGATCTTTCGGTAATGGAAAAGCAAGAAGCCGACGCTATCATGCGTCCTTTTTCCTTTGCT
CACGTGGAAATCAATCGTCCACATGCGGACTGTTATATTAATTACACCAATGAGAGAACGCATCAGCTCATCCGTGAGAA
TTTTCATCGTTCTCCATTCTTTTCTGGCAGAATAAAGGCGGTAGGGACACGATATTGTCCGTCTATCGAAGATAAAGTGA
GGAAGTTCCCCGACAGGATACGTCACCAGCTGTACATAGAACCGGAAGGATTAGATACGGAAGAGTTATATATAAACGGG
CTTTCATCCTGTTTGCCAGAGGATATACAGGATGAAATGATCCGCACTATCCCTGGTATGGAACGCGCGGTTATTACGCG
TCCTGCATACGCGGTGGATTATGCGGTGCTATTCCCTGTACAACTTGGTATTGATTTGCAAACAAAAAGGGTGAGCGGGC
TCTTTTCTGCAGGTCAGATTAACGGAACATCCGGCTATGAAGAAGCTGGAGGTCAGGGTATTATCGCCGGGATTAACGCT
GCGCTGTACGCGCGCAGTACTAAAACCAAAGAGGAGTATCATCCATTTGTTCTGAAACGCGACGAAGCATATATTGGCGT
CATGATAGATGATCTTGTAACACAAGGAATAGACGAACCCTATCGGATGTTTACCGCGCGTGCGGAGTATCGTTTGAAAC
TCCGTCACGATACTGCGGATGAACGTCTTACAGAAAAAGCTTACGCCATTGGGCTGCAGAAGAAATCTGCTGTAGAAACG
TTGCAAAAAAAGATGCGTACGAAGCACGAGATCTTGCATCTGCTTCAGACCAACAAAGTTAGTCTTACCCATGCAAACGC
ATATGTTCAGCTGAAGCCGCATATAGGTAAATCGTTTGCAGCTACGCTACGTGATCCGGTAATACCTCTTGGGCTTATCG
CTTCGCTGAACGAGCAGATAGCGCAGTTCCCTTTGGAAGTGTTCCAGTCGGTTGGGGTGGAGATACGCTACGAACACTAC
ATCGCTGCACAGGATCAAAGAATTGCACAAGTGGAGAAAATGGAAGGAATAAAGATACCAGCGCATTTTGATTACGCGCG
TATATCAGGTCTCTCTGTAGAATCCCGTACACGATTGGAACACGTTCGCCCGGACACTATCGGGCAGGTTGGGAGAATGC
GCGGAATCAGACCCTCTGACGTAATGCTGTTGCTCGCCCACTTAAAGCGGTAG

Upstream 100 bases:

>100_bases
TCAGAAACAGGCTTCAGATGTTGTGTGTGCGTTGCTTCCTGAATTCTGTCGTGCTTCCTGATTGAAGCCTTTTGCACCTT
TCTTCTATAATCCGCTGCCC

Downstream 100 bases:

>100_bases
CAGCTACCGCAGAGATAGAAGAACCGCCTTATCAGGCAGGTGTTTGTACGTACTTTTAACGCACAGCAAGGAGCGCTTCG
GCGTGAAGTTCGGTGATAAG

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A

Number of amino acids: Translated: 630; Mature: 629

Protein sequence:

>630_residues
MGFRFSDYDVIVVGGGHAGAEAALAAARMGEHTLLITQTIDSIGRLSCNPSIGGISKGNIVREIDALGGEMGKFADACMI
QYRLLNKSRGPAVQAPRIQADKFLYAQKVKYTLECTQHLHLYQDTVVDVVCSNTTDAGYVAYGAAHAVVTARGRRISARA
VVLTTGTFMEGRVYIGEYEAPEGRLGEHAAEGLGAALRKKGFQMGRLKTGTPARVLRKSVDLSVMEKQEADAIMRPFSFA
HVEINRPHADCYINYTNERTHQLIRENFHRSPFFSGRIKAVGTRYCPSIEDKVRKFPDRIRHQLYIEPEGLDTEELYING
LSSCLPEDIQDEMIRTIPGMERAVITRPAYAVDYAVLFPVQLGIDLQTKRVSGLFSAGQINGTSGYEEAGGQGIIAGINA
ALYARSTKTKEEYHPFVLKRDEAYIGVMIDDLVTQGIDEPYRMFTARAEYRLKLRHDTADERLTEKAYAIGLQKKSAVET
LQKKMRTKHEILHLLQTNKVSLTHANAYVQLKPHIGKSFAATLRDPVIPLGLIASLNEQIAQFPLEVFQSVGVEIRYEHY
IAAQDQRIAQVEKMEGIKIPAHFDYARISGLSVESRTRLEHVRPDTIGQVGRMRGIRPSDVMLLLAHLKR

Sequences:

>Translated_630_residues
MGFRFSDYDVIVVGGGHAGAEAALAAARMGEHTLLITQTIDSIGRLSCNPSIGGISKGNIVREIDALGGEMGKFADACMI
QYRLLNKSRGPAVQAPRIQADKFLYAQKVKYTLECTQHLHLYQDTVVDVVCSNTTDAGYVAYGAAHAVVTARGRRISARA
VVLTTGTFMEGRVYIGEYEAPEGRLGEHAAEGLGAALRKKGFQMGRLKTGTPARVLRKSVDLSVMEKQEADAIMRPFSFA
HVEINRPHADCYINYTNERTHQLIRENFHRSPFFSGRIKAVGTRYCPSIEDKVRKFPDRIRHQLYIEPEGLDTEELYING
LSSCLPEDIQDEMIRTIPGMERAVITRPAYAVDYAVLFPVQLGIDLQTKRVSGLFSAGQINGTSGYEEAGGQGIIAGINA
ALYARSTKTKEEYHPFVLKRDEAYIGVMIDDLVTQGIDEPYRMFTARAEYRLKLRHDTADERLTEKAYAIGLQKKSAVET
LQKKMRTKHEILHLLQTNKVSLTHANAYVQLKPHIGKSFAATLRDPVIPLGLIASLNEQIAQFPLEVFQSVGVEIRYEHY
IAAQDQRIAQVEKMEGIKIPAHFDYARISGLSVESRTRLEHVRPDTIGQVGRMRGIRPSDVMLLLAHLKR
>Mature_629_residues
GFRFSDYDVIVVGGGHAGAEAALAAARMGEHTLLITQTIDSIGRLSCNPSIGGISKGNIVREIDALGGEMGKFADACMIQ
YRLLNKSRGPAVQAPRIQADKFLYAQKVKYTLECTQHLHLYQDTVVDVVCSNTTDAGYVAYGAAHAVVTARGRRISARAV
VLTTGTFMEGRVYIGEYEAPEGRLGEHAAEGLGAALRKKGFQMGRLKTGTPARVLRKSVDLSVMEKQEADAIMRPFSFAH
VEINRPHADCYINYTNERTHQLIRENFHRSPFFSGRIKAVGTRYCPSIEDKVRKFPDRIRHQLYIEPEGLDTEELYINGL
SSCLPEDIQDEMIRTIPGMERAVITRPAYAVDYAVLFPVQLGIDLQTKRVSGLFSAGQINGTSGYEEAGGQGIIAGINAA
LYARSTKTKEEYHPFVLKRDEAYIGVMIDDLVTQGIDEPYRMFTARAEYRLKLRHDTADERLTEKAYAIGLQKKSAVETL
QKKMRTKHEILHLLQTNKVSLTHANAYVQLKPHIGKSFAATLRDPVIPLGLIASLNEQIAQFPLEVFQSVGVEIRYEHYI
AAQDQRIAQVEKMEGIKIPAHFDYARISGLSVESRTRLEHVRPDTIGQVGRMRGIRPSDVMLLLAHLKR

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family

Homologues:

Organism=Homo sapiens, GI74024895, Length=648, Percent_Identity=39.3518518518519, Blast_Score=424, Evalue=1e-118,
Organism=Homo sapiens, GI19882217, Length=673, Percent_Identity=37.890044576523, Blast_Score=410, Evalue=1e-114,
Organism=Homo sapiens, GI183227703, Length=688, Percent_Identity=37.0639534883721, Blast_Score=404, Evalue=1e-112,
Organism=Escherichia coli, GI2367273, Length=636, Percent_Identity=43.3962264150943, Blast_Score=506, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI17534255, Length=647, Percent_Identity=36.1669242658423, Blast_Score=397, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6321202, Length=622, Percent_Identity=41.1575562700965, Blast_Score=470, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24658174, Length=642, Percent_Identity=40.0311526479751, Blast_Score=437, Evalue=1e-122,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMG_TREPA (O83084)

Other databases:

- EMBL:   AE000520
- PIR:   C71374
- RefSeq:   NP_218484.1
- ProteinModelPortal:   O83084
- SMR:   O83084
- IntAct:   O83084
- GeneID:   2610776
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0044
- NMPDR:   fig|243276.1.peg.43
- TIGR:   TP_0044
- HOGENOM:   HBG284774
- OMA:   GIQFRVL
- ProtClustDB:   PRK05192
- BioCyc:   TPAL243276:TP_0044-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00129
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00136

Pfam domain/function: PF01134 GIDA

EC number: NA

Molecular weight: Translated: 70177; Mature: 70045

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFRFSDYDVIVVGGGHAGAEAALAAARMGEHTLLITQTIDSIGRLSCNPSIGGISKGNI
CCCEECCEEEEEEECCCCCHHHHHHHHHCCCCEEEEEEHHHHHHCEECCCCCCCCCCCCH
VREIDALGGEMGKFADACMIQYRLLNKSRGPAVQAPRIQADKFLYAQKVKYTLECTQHLH
HHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LYQDTVVDVVCSNTTDAGYVAYGAAHAVVTARGRRISARAVVLTTGTFMEGRVYIGEYEA
HHHHHHHHHEECCCCCCCEEEECHHEEEEEECCCEEEEEEEEEEECCEEECEEEEECCCC
PEGRLGEHAAEGLGAALRKKGFQMGRLKTGTPARVLRKSVDLSVMEKQEADAIMRPFSFA
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCEEE
HVEINRPHADCYINYTNERTHQLIRENFHRSPFFSGRIKAVGTRYCPSIEDKVRKFPDRI
EEEEECCCCEEEEEECCHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHH
RHQLYIEPEGLDTEELYINGLSSCLPEDIQDEMIRTIPGMERAVITRPAYAVDYAVLFPV
CCEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHEEECCCHHHHEEEEEEE
QLGIDLQTKRVSGLFSAGQINGTSGYEEAGGQGIIAGINAALYARSTKTKEEYHPFVLKR
EECCEEEHHHHHCCCCCCCCCCCCCCHHCCCCEEEEECCEEEEECCCCCHHHCCCEEEEC
DEAYIGVMIDDLVTQGIDEPYRMFTARAEYRLKLRHDTADERLTEKAYAIGLQKKSAVET
CCCEEEEEHHHHHHHCCCCHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHCCCHHHHHHH
LQKKMRTKHEILHLLQTNKVSLTHANAYVQLKPHIGKSFAATLRDPVIPLGLIASLNEQI
HHHHHHHHHHHHHHHHCCCEEEEECCEEEEECCCCCCHHHHHHHCCCCCHHHHHHHHHHH
AQFPLEVFQSVGVEIRYEHYIAAQDQRIAQVEKMEGIKIPAHFDYARISGLSVESRTRLE
HHHHHHHHHHCCCEEEEEEEEECCCHHHHHHHHHCCEECCCCCCHHHHCCCCCHHHHHHH
HVRPDTIGQVGRMRGIRPSDVMLLLAHLKR
HCCCCHHHHHHHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
GFRFSDYDVIVVGGGHAGAEAALAAARMGEHTLLITQTIDSIGRLSCNPSIGGISKGNI
CCEECCEEEEEEECCCCCHHHHHHHHHCCCCEEEEEEHHHHHHCEECCCCCCCCCCCCH
VREIDALGGEMGKFADACMIQYRLLNKSRGPAVQAPRIQADKFLYAQKVKYTLECTQHLH
HHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LYQDTVVDVVCSNTTDAGYVAYGAAHAVVTARGRRISARAVVLTTGTFMEGRVYIGEYEA
HHHHHHHHHEECCCCCCCEEEECHHEEEEEECCCEEEEEEEEEEECCEEECEEEEECCCC
PEGRLGEHAAEGLGAALRKKGFQMGRLKTGTPARVLRKSVDLSVMEKQEADAIMRPFSFA
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCEEE
HVEINRPHADCYINYTNERTHQLIRENFHRSPFFSGRIKAVGTRYCPSIEDKVRKFPDRI
EEEEECCCCEEEEEECCHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHH
RHQLYIEPEGLDTEELYINGLSSCLPEDIQDEMIRTIPGMERAVITRPAYAVDYAVLFPV
CCEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHEEECCCHHHHEEEEEEE
QLGIDLQTKRVSGLFSAGQINGTSGYEEAGGQGIIAGINAALYARSTKTKEEYHPFVLKR
EECCEEEHHHHHCCCCCCCCCCCCCCHHCCCCEEEEECCEEEEECCCCCHHHCCCEEEEC
DEAYIGVMIDDLVTQGIDEPYRMFTARAEYRLKLRHDTADERLTEKAYAIGLQKKSAVET
CCCEEEEEHHHHHHHCCCCHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHCCCHHHHHHH
LQKKMRTKHEILHLLQTNKVSLTHANAYVQLKPHIGKSFAATLRDPVIPLGLIASLNEQI
HHHHHHHHHHHHHHHHCCCEEEEECCEEEEECCCCCCHHHHHHHCCCCCHHHHHHHHHHH
AQFPLEVFQSVGVEIRYEHYIAAQDQRIAQVEKMEGIKIPAHFDYARISGLSVESRTRLE
HHHHHHHHHHCCCEEEEEEEEECCCHHHHHHHHHCCEECCCCCCHHHHCCCCCHHHHHHH
HVRPDTIGQVGRMRGIRPSDVMLLLAHLKR
HCCCCHHHHHHHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876