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The map label for this gene is dnaN

Identifier: 189025238

GI number: 189025238

Start: 1641

End: 2756

Strand: Direct

Name: dnaN

Synonym: TPASS_0002

Alternate gene names: 189025238

Gene position: 1641-2756 (Clockwise)

Preceding gene: 189025237

Following gene: 189025239

Centisome position: 0.14

GC content: 41.31

Gene sequence:

>1116_bases
GTGAAAATCCTATGCGAGAAAGAAGCCTTTCTGAAGGAAATAAGCACAGCACAAGAGGTTATTTCAAATAAAAAAAACAC
GTCTATTTTTTCGAACGTCCTATTAGCTGCTCAAGGAGCCCTGCTTACCATCAGAGCAACCGACACAAAAGTTACCTTTG
AAACTAGCATTCCCGTCAATGTTCTCGCCGAAGGCACAACGACAGTTTTTTGCGACAAACTTGTGAATGTTGTTTCTGCC
CTTCCAACAAAAGAAATCGAATTAACGTTATGTGAAGAACAACTTGTCATTACCCCTCCAAACAAAAAGATAAGCTTTCA
GCTCAGAACCCTCTCGCATGAGAGTTTTCCATGTTTCCCTCAAAATGAAGGAGGCGTCTCTCTTGCTGTGCCTACCTCCG
ATCTTAGAAACATGATTAACCATACCGTTTTTGCAGTTTCAGAAGACAGTACGCGCCATTTTATCAATGGCGTACACGTT
GATTTTCAGTATGGAAATATTATTTGTGTTTCAACAGATGGAAAGCGGCTCGCCTATATAGAAAAAAAGGGAGAATCCTC
TCCCCAATCCTTTTCGGGTGTTATTGTGCCAACTAAGATCTTAGGCATAGTAAACCGTAAGCTTACCCCTGAAGGATCAG
TGACGCTATGCATTACGTCGCAGCACGTTTACTTTTTTTTCGGTGGATATAAGTTTTCTTCTGTGCTTATTGAGGGGCAA
TTTCCTAATTACAAAAGAGTAATCCCTGATCATCAGGAGCGTTCTTTTTGTGTTGGACGTGTGGAGCTAATGGAGGCACT
TAAACGAGTCTCGTTGTTGGTAGAACAAAAATCTCACAGGATATTTATTACCATACAGCAGGGTTTGTTGACTTTAAGCT
CAAAAGCTCACACTCAAGAAAATGAAATAGGTGATGCTCAGGAAGAAATAGCCTGTGCTTATACAGGAGAAAGTGAGGTC
ATAGCTCTTAACTATCTATACCTTGAAGAACCGCTTAAGGTTTTTACTTCGAAGGAGGTTCAAGTGGAATTTACCGATCC
TGCAAAAGCACTCACGCTTCGTGCTGTACCAAACACGGACTGCTTTCACATCATTATGCCTATGCAAACGGAGTGA

Upstream 100 bases:

>100_bases
ATTCTCGAGACATAAGCACTTATCCAGATATTCACAGTTACTATTATGTGATACGACTACATTCTTTATACTTATAAGAT
TAATAAGGAGGAAACTAACT

Downstream 100 bases:

>100_bases
TTCTTTGCCTTTTCTCACAGTGACTGCAATAAATTTCAGAAATCTTGCACATCACACGATTGATATATCCTCTCCTGAGG
TTTTTTTTGTGGGAAATAAC

Product: DNA-directed DNA polymerase beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MKILCEKEAFLKEISTAQEVISNKKNTSIFSNVLLAAQGALLTIRATDTKVTFETSIPVNVLAEGTTTVFCDKLVNVVSA
LPTKEIELTLCEEQLVITPPNKKISFQLRTLSHESFPCFPQNEGGVSLAVPTSDLRNMINHTVFAVSEDSTRHFINGVHV
DFQYGNIICVSTDGKRLAYIEKKGESSPQSFSGVIVPTKILGIVNRKLTPEGSVTLCITSQHVYFFFGGYKFSSVLIEGQ
FPNYKRVIPDHQERSFCVGRVELMEALKRVSLLVEQKSHRIFITIQQGLLTLSSKAHTQENEIGDAQEEIACAYTGESEV
IALNYLYLEEPLKVFTSKEVQVEFTDPAKALTLRAVPNTDCFHIIMPMQTE

Sequences:

>Translated_371_residues
MKILCEKEAFLKEISTAQEVISNKKNTSIFSNVLLAAQGALLTIRATDTKVTFETSIPVNVLAEGTTTVFCDKLVNVVSA
LPTKEIELTLCEEQLVITPPNKKISFQLRTLSHESFPCFPQNEGGVSLAVPTSDLRNMINHTVFAVSEDSTRHFINGVHV
DFQYGNIICVSTDGKRLAYIEKKGESSPQSFSGVIVPTKILGIVNRKLTPEGSVTLCITSQHVYFFFGGYKFSSVLIEGQ
FPNYKRVIPDHQERSFCVGRVELMEALKRVSLLVEQKSHRIFITIQQGLLTLSSKAHTQENEIGDAQEEIACAYTGESEV
IALNYLYLEEPLKVFTSKEVQVEFTDPAKALTLRAVPNTDCFHIIMPMQTE
>Mature_371_residues
MKILCEKEAFLKEISTAQEVISNKKNTSIFSNVLLAAQGALLTIRATDTKVTFETSIPVNVLAEGTTTVFCDKLVNVVSA
LPTKEIELTLCEEQLVITPPNKKISFQLRTLSHESFPCFPQNEGGVSLAVPTSDLRNMINHTVFAVSEDSTRHFINGVHV
DFQYGNIICVSTDGKRLAYIEKKGESSPQSFSGVIVPTKILGIVNRKLTPEGSVTLCITSQHVYFFFGGYKFSSVLIEGQ
FPNYKRVIPDHQERSFCVGRVELMEALKRVSLLVEQKSHRIFITIQQGLLTLSSKAHTQENEIGDAQEEIACAYTGESEV
IALNYLYLEEPLKVFTSKEVQVEFTDPAKALTLRAVPNTDCFHIIMPMQTE

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=375, Percent_Identity=24.5333333333333, Blast_Score=127, Evalue=1e-30,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): DPO3B_TREPA (O83048)

Other databases:

- EMBL:   AE000520
- PIR:   A71378
- RefSeq:   NP_218443.1
- ProteinModelPortal:   O83048
- IntAct:   O83048
- MINT:   MINT-6479952
- GeneID:   2611815
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0002
- NMPDR:   fig|243276.1.peg.2
- TIGR:   TP_0002
- HOGENOM:   HBG731928
- OMA:   DYNRVIP
- ProtClustDB:   PRK14940
- BioCyc:   TPAL243276:TP_0002-MONOMER
- BRENDA:   2.7.7.7
- GO:   GO:0005737
- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634
- Gene3D:   G3DSA:3.10.150.10
- SMART:   SM00480
- TIGRFAMs:   TIGR00663

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3

EC number: =2.7.7.7

Molecular weight: Translated: 41291; Mature: 41291

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILCEKEAFLKEISTAQEVISNKKNTSIFSNVLLAAQGALLTIRATDTKVTFETSIPVN
CCEEECHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEECCEEEEEEECCCEE
VLAEGTTTVFCDKLVNVVSALPTKEIELTLCEEQLVITPPNKKISFQLRTLSHESFPCFP
EEECCCHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEECCCCEEEEEEEEECCCCCCCCC
QNEGGVSLAVPTSDLRNMINHTVFAVSEDSTRHFINGVHVDFQYGNIICVSTDGKRLAYI
CCCCCEEEEECHHHHHHHHHCEEEEEECCCCCHHHCEEEEEEEECCEEEEECCCCEEEEE
EKKGESSPQSFSGVIVPTKILGIVNRKLTPEGSVTLCITSQHVYFFFGGYKFSSVLIEGQ
EECCCCCCCCCCCEEEHHHHHHHHCCCCCCCCCEEEEEECCEEEEEEECEEEEEEEEECC
FPNYKRVIPDHQERSFCVGRVELMEALKRVSLLVEQKSHRIFITIQQGLLTLSSKAHTQE
CCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCEEEECCCCCCCC
NEIGDAQEEIACAYTGESEVIALNYLYLEEPLKVFTSKEVQVEFTDPAKALTLRAVPNTD
CCCCCCHHHEEEEECCCCCEEEEEEEEECCHHHHHCCCEEEEEECCCCCEEEEEECCCCC
CFHIIMPMQTE
EEEEEEEECCC
>Mature Secondary Structure
MKILCEKEAFLKEISTAQEVISNKKNTSIFSNVLLAAQGALLTIRATDTKVTFETSIPVN
CCEEECHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEECCEEEEEEECCCEE
VLAEGTTTVFCDKLVNVVSALPTKEIELTLCEEQLVITPPNKKISFQLRTLSHESFPCFP
EEECCCHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEECCCCEEEEEEEEECCCCCCCCC
QNEGGVSLAVPTSDLRNMINHTVFAVSEDSTRHFINGVHVDFQYGNIICVSTDGKRLAYI
CCCCCEEEEECHHHHHHHHHCEEEEEECCCCCHHHCEEEEEEEECCEEEEECCCCEEEEE
EKKGESSPQSFSGVIVPTKILGIVNRKLTPEGSVTLCITSQHVYFFFGGYKFSSVLIEGQ
EECCCCCCCCCCCEEEHHHHHHHHCCCCCCCCCEEEEEECCEEEEEEECEEEEEEEEECC
FPNYKRVIPDHQERSFCVGRVELMEALKRVSLLVEQKSHRIFITIQQGLLTLSSKAHTQE
CCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCEEEECCCCCCCC
NEIGDAQEEIACAYTGESEVIALNYLYLEEPLKVFTSKEVQVEFTDPAKALTLRAVPNTD
CCCCCCHHHEEEEECCCCCEEEEEEEEECCHHHHHCCCEEEEEECCCCCEEEEEECCCCC
CFHIIMPMQTE
EEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876