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The map label for this gene is recF [H]

Identifier: 189025239

GI number: 189025239

Start: 2623

End: 3834

Strand: Direct

Name: recF [H]

Synonym: TPASS_0003

Alternate gene names: 189025239

Gene position: 2623-3834 (Clockwise)

Preceding gene: 189025238

Following gene: 189025240

Centisome position: 0.23

GC content: 43.56

Gene sequence:

>1212_bases
TTGAAGAACCGCTTAAGGTTTTTACTTCGAAGGAGGTTCAAGTGGAATTTACCGATCCTGCAAAAGCACTCACGCTTCGT
GCTGTACCAAACACGGACTGCTTTCACATCATTATGCCTATGCAAACGGAGTGATTCTTTGCCTTTTCTCACAGTGACTG
CAATAAATTTCAGAAATCTTGCACATCACACGATTGATATATCCTCTCCTGAGGTTTTTTTTGTGGGAAATAACGGACAG
GGAAAAACCAATATACTTGAGGTTCTATATCTTGCTGCGTACGGAAATTCGTTTCGAACACGCACCGAAAGCGAACTGTA
TGCAACTCACGCGCGTTCGAATGAGTATCGGGTAAAAGTTATGTACCGCGGGGAGTATACCCACACAGTGCAGATTTTCT
CCAAAAATGGAAAAAAGCGCATTGAGAAAAACTTGAAAAAAATAAGGACAAAAAAAGAACTTATCAGCAGTATTCCCTGT
ATTTTGTTTTTTCATAACGATTTGGACTTCGTAGTTGGTACGCCAGAACGCAGACGCTTCTTTTTGGATCAATCCCTTTC
GATGTGTAATCCTCTGTATTTGGAATACTTGCAAAAATATCACGCACTAACAAAAACAAAGAACAGAGAGATAAAAGAGA
AACGCGTTCAGTTACTCGATGCACTGGATACGCAAATTGCAACCGTGGGTTTTGATCTCGTGCAGTGGAGAACTCAGCTT
GTCCGTGACTTTAACGTGATTTTTACTAAGTATTATGAGCGCCTTGGAGACCTTGCGCAGGTGCGCATTGAGTATAAGCC
TTCATGGTCTGACTCCTCAGTTGAGGAGATCGTACATTCTCTTTACAAGAGACGTAAGCACGATCTTGCGATGGGGATGA
GTATGTCAGGTCCTCATAGAGATAAGATTCACTTTACTCGGTCGCAGGCGCTTTTCATTCCTCAGGCTTCTACCGGACAG
AGGCGGTTGGTTTCGTTGGTACTGAGGATGTCGCAGGCTGTGTTCTACACAGGAGTAACGGGAAAACTGCCCGTACTCTT
AATGGATGATGTCTTGTTAGAGCTTGATCCTGAGAAGCGGGAAAGGTTCATGATGAGTTTGCCTCCGTATGATCAGCTGT
TTTGTACATTTTTGCCAGGGGAAGCGTACAGGCGATACGGGCGTGAAAAAACGCGGGTATATTTTGTTTCTGAAGGGGCG
TGTCATGAATAA

Upstream 100 bases:

>100_bases
AAAGCTCACACTCAAGAAAATGAAATAGGTGATGCTCAGGAAGAAATAGCCTGTGCTTATACAGGAGAAAGTGAGGTCAT
AGCTCTTAACTATCTATACC

Downstream 100 bases:

>100_bases
TGGTGTGAATAAGCTATCGGACTTACTCGTGTTGACCACTGAATATATCCAAGCTTCCTATGAAACGGAGGCGTTTGATG
CGCATCGAGAATGGGTGTGT

Product: recombination protein RecF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MKNRLRFLLRRRFKWNLPILQKHSRFVLYQTRTAFTSLCLCKRSDSLPFLTVTAINFRNLAHHTIDISSPEVFFVGNNGQ
GKTNILEVLYLAAYGNSFRTRTESELYATHARSNEYRVKVMYRGEYTHTVQIFSKNGKKRIEKNLKKIRTKKELISSIPC
ILFFHNDLDFVVGTPERRRFFLDQSLSMCNPLYLEYLQKYHALTKTKNREIKEKRVQLLDALDTQIATVGFDLVQWRTQL
VRDFNVIFTKYYERLGDLAQVRIEYKPSWSDSSVEEIVHSLYKRRKHDLAMGMSMSGPHRDKIHFTRSQALFIPQASTGQ
RRLVSLVLRMSQAVFYTGVTGKLPVLLMDDVLLELDPEKRERFMMSLPPYDQLFCTFLPGEAYRRYGREKTRVYFVSEGA
CHE

Sequences:

>Translated_403_residues
MKNRLRFLLRRRFKWNLPILQKHSRFVLYQTRTAFTSLCLCKRSDSLPFLTVTAINFRNLAHHTIDISSPEVFFVGNNGQ
GKTNILEVLYLAAYGNSFRTRTESELYATHARSNEYRVKVMYRGEYTHTVQIFSKNGKKRIEKNLKKIRTKKELISSIPC
ILFFHNDLDFVVGTPERRRFFLDQSLSMCNPLYLEYLQKYHALTKTKNREIKEKRVQLLDALDTQIATVGFDLVQWRTQL
VRDFNVIFTKYYERLGDLAQVRIEYKPSWSDSSVEEIVHSLYKRRKHDLAMGMSMSGPHRDKIHFTRSQALFIPQASTGQ
RRLVSLVLRMSQAVFYTGVTGKLPVLLMDDVLLELDPEKRERFMMSLPPYDQLFCTFLPGEAYRRYGREKTRVYFVSEGA
CHE
>Mature_403_residues
MKNRLRFLLRRRFKWNLPILQKHSRFVLYQTRTAFTSLCLCKRSDSLPFLTVTAINFRNLAHHTIDISSPEVFFVGNNGQ
GKTNILEVLYLAAYGNSFRTRTESELYATHARSNEYRVKVMYRGEYTHTVQIFSKNGKKRIEKNLKKIRTKKELISSIPC
ILFFHNDLDFVVGTPERRRFFLDQSLSMCNPLYLEYLQKYHALTKTKNREIKEKRVQLLDALDTQIATVGFDLVQWRTQL
VRDFNVIFTKYYERLGDLAQVRIEYKPSWSDSSVEEIVHSLYKRRKHDLAMGMSMSGPHRDKIHFTRSQALFIPQASTGQ
RRLVSLVLRMSQAVFYTGVTGKLPVLLMDDVLLELDPEKRERFMMSLPPYDQLFCTFLPGEAYRRYGREKTRVYFVSEGA
CHE

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=355, Percent_Identity=22.8169014084507, Blast_Score=92, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 47468; Mature: 47468

Theoretical pI: Translated: 10.29; Mature: 10.29

Prosite motif: PS00617 RECF_1 ; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNRLRFLLRRRFKWNLPILQKHSRFVLYQTRTAFTSLCLCKRSDSLPFLTVTAINFRNL
CCHHHHHHHHHHHCCCCCHHHCCCEEEEEEEHHHHHHHHHHCCCCCCCEEEEEEECHHHH
AHHTIDISSPEVFFVGNNGQGKTNILEVLYLAAYGNSFRTRTESELYATHARSNEYRVKV
HEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCEEEEE
MYRGEYTHTVQIFSKNGKKRIEKNLKKIRTKKELISSIPCILFFHNDLDFVVGTPERRRF
EEECCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEECCCHHHHH
FLDQSLSMCNPLYLEYLQKYHALTKTKNREIKEKRVQLLDALDTQIATVGFDLVQWRTQL
HHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VRDFNVIFTKYYERLGDLAQVRIEYKPSWSDSSVEEIVHSLYKRRKHDLAMGMSMSGPHR
HHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
DKIHFTRSQALFIPQASTGQRRLVSLVLRMSQAVFYTGVTGKLPVLLMDDVLLELDPEKR
CCEEEECCCEEEECCCCCCHHHHHHHHHHHHHHHEEECCCCCCCCEEECCHHHCCCCHHH
ERFMMSLPPYDQLFCTFLPGEAYRRYGREKTRVYFVSEGACHE
HHHEECCCCHHHHEEEECCCHHHHHCCCCCEEEEEEECCCCCC
>Mature Secondary Structure
MKNRLRFLLRRRFKWNLPILQKHSRFVLYQTRTAFTSLCLCKRSDSLPFLTVTAINFRNL
CCHHHHHHHHHHHCCCCCHHHCCCEEEEEEEHHHHHHHHHHCCCCCCCEEEEEEECHHHH
AHHTIDISSPEVFFVGNNGQGKTNILEVLYLAAYGNSFRTRTESELYATHARSNEYRVKV
HEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCEEEEE
MYRGEYTHTVQIFSKNGKKRIEKNLKKIRTKKELISSIPCILFFHNDLDFVVGTPERRRF
EEECCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEECCCHHHHH
FLDQSLSMCNPLYLEYLQKYHALTKTKNREIKEKRVQLLDALDTQIATVGFDLVQWRTQL
HHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VRDFNVIFTKYYERLGDLAQVRIEYKPSWSDSSVEEIVHSLYKRRKHDLAMGMSMSGPHR
HHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
DKIHFTRSQALFIPQASTGQRRLVSLVLRMSQAVFYTGVTGKLPVLLMDDVLLELDPEKR
CCEEEECCCEEEECCCCCCHHHHHHHHHHHHHHHEEECCCCCCCCEEECCHHHCCCCHHH
ERFMMSLPPYDQLFCTFLPGEAYRRYGREKTRVYFVSEGACHE
HHHEECCCCHHHHEEEECCCHHHHHCCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]